FI113785B - Transformering av Phaffia rhodozyma - Google Patents

Transformering av Phaffia rhodozyma Download PDF

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Publication number
FI113785B
FI113785B FI933993A FI933993A FI113785B FI 113785 B FI113785 B FI 113785B FI 933993 A FI933993 A FI 933993A FI 933993 A FI933993 A FI 933993A FI 113785 B FI113785 B FI 113785B
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FI
Finland
Prior art keywords
phaffia
dna
cells
gene
promoter
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Application number
FI933993A
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English (en)
Finnish (fi)
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FI933993A0 (fi
FI933993A (fi
Inventor
Ooyen Albert Johannes Jose Van
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Dsm Ip Assets Bv
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Application filed by Dsm Ip Assets Bv filed Critical Dsm Ip Assets Bv
Publication of FI933993A0 publication Critical patent/FI933993A0/fi
Publication of FI933993A publication Critical patent/FI933993A/fi
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Publication of FI113785B publication Critical patent/FI113785B/sv

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C07K14/39—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Mycology (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Coils Or Transformers For Communication (AREA)
  • Magnetic Ceramics (AREA)

Claims (12)

1. Phaffia-cell transformerad med DNA, kännetecnad därav, att nämnda DNA omfattar 5 a) en i nämnda Phaffia-cell uttryckande önskad gen, b) en Phaffia-promotor heterologisk för nämnda önskade uttryckande gen och operabelt kopplad darmed. 10
2. Phaffia-cell enligt patentkravet 1, kännetecknad därav, att Phaffia-promotorn är en aktinpromotor.
3. Phaffia-cell enligt patentkravet 1 eller 2, kännetecknad 15 därav, att nämnda DNA ytterligare omfattar en selektiv markör.
4. Phaffia rhodozyma CBS 303.93.
20 S. E. coli CBS 359.93.
6. E. coli CBS 442.92. * 7. Förfarande för att transformera Phaffia, kännetecknat 25 därav, att k * » * " Phaffia-celler odlas tili det eksponentiella steget, » ’ I - protoplaster tillverkas av dessa celler, 30. en vektor tillsätts, som innehäller en önskad gen klonad nedströms frän en Phaffia-promotor ! * heterologisk för den, :·’ · - protoplasterna plattas pä ett selektivt regenerationsmedium, 113785 - de transformerade Phaffia-cellerna väljs.
8. Förfarande för att transformera Phaffia, kännetecknat därav, att 5 - Phaffia-celler odlas tili önskad densitet, - cellerna tillvaratas, - cellerna inkuberas i närvaro av litiumacetat med transformerande DNA, som omfattar en önskad gen 10 klonad nedströms frän en Phaffia-promotor, - blandningen breds pä selektiva plattor, de transformerade Phaffia-cellerna väljs.
9. Förfarande för att producera Phaffia-celler, som 15 innehäller en mängd av ett önskat protein eller karotenoid, kännetecknat därav, att - Phaffia-celler transformeras med en vektor, som innehäller en önskad gen, som kan vara en protein 20 kodande gen eller regleringsgen klonad nedströms frän en Phaf f ia-promotor, - de transf ormerade cellerna väljs, • - de transformerade cellerna odlas under lämpliga förhällanden för att producera det önskade • : 25 proteinet eller karotenoiden.
10. Förfarande för att framställa en önskad produkt, kännetecknat därav, att transformerade Phaffia-celler enligt nägot av patentkraven 1-3 odlas under lämpliga förhällanden : 30 för att framställa nämnda önskade produkt.
11. Förfarande enligt patentkravet 10, kännetecknat därav, att den önskade produkten är ett protein. t 57 1 13785
12. Förfarande enligt patentkravet 10, kännetecknat därav, att den önskade produkten är karotenoid. 1 · i
FI933993A 1992-09-11 1993-09-10 Transformering av Phaffia rhodozyma FI113785B (sv)

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
EP92202789 1992-09-11
EP92202789 1992-09-11
EP92203313 1992-10-28
EP92203313 1992-10-28
EP93201848 1993-06-25
EP93201848 1993-06-25

Publications (3)

Publication Number Publication Date
FI933993A0 FI933993A0 (fi) 1993-09-10
FI933993A FI933993A (fi) 1994-03-12
FI113785B true FI113785B (sv) 2004-06-15

Family

ID=27234645

Family Applications (1)

Application Number Title Priority Date Filing Date
FI933993A FI113785B (sv) 1992-09-11 1993-09-10 Transformering av Phaffia rhodozyma

Country Status (12)

Country Link
US (1) US5840528A (sv)
EP (1) EP0590707B1 (sv)
JP (1) JPH07501225A (sv)
AT (1) ATE228567T1 (sv)
AU (1) AU673847B2 (sv)
CA (1) CA2105957C (sv)
DE (1) DE69332514T2 (sv)
DK (1) DK0590707T3 (sv)
FI (1) FI113785B (sv)
NO (1) NO313840B1 (sv)
NZ (1) NZ248628A (sv)
WO (1) WO1994006918A2 (sv)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1203818A3 (en) * 1993-12-27 2002-06-12 Kirin Beer Kabushiki Kaisha DNA Strands useful for the synthesis of xanthophylls and the process for producing the xanthophylls
KR0178871B1 (ko) * 1994-08-23 1999-04-01 게이사쿠 마나베 케토기 도입 효소, 그를 암호화하는 dna 및 케토카로테노이드를제조하는방법
US5910433A (en) * 1994-08-23 1999-06-08 Kirin Beer Kabushiki Kaisha Keto group-introducing enzyme, DNA coding therefor and method for producing ketocarotenoids
JP2000507087A (ja) 1995-12-22 2000-06-13 ギスト ブロカデス ベスローテン ファンノートシャップ ファフィア株を形質転換する改良法、そのようにして得られた形質転換したファフィア株及び前記方法における組換えｄｎａ
TWI250210B (en) 1998-05-06 2006-03-01 Dsm Ip Assets Bv An isolated DNA sequence coding for an enzyme involved in the mevalonate pathway or the pathway from isopentenyl pyrophosphate to farnesyl pyrophosphate
KR100316741B1 (ko) 1998-10-31 2002-09-04 한국생명공학연구원 파피아로도지마형질전환용벡터및그형질전환방법
US6924359B1 (en) * 1999-07-01 2005-08-02 Yale University Neovascular-targeted immunoconjugates
CA2324625C (en) 1999-12-01 2011-09-27 F. Hoffmann-La Roche Ag Recombinant production of carotenoids, particularly astaxanthin
ATE312928T1 (de) * 2000-05-24 2005-12-15 Dsm Ip Assets Bv Verfahren zur herstellung von astaxanthin
WO2004029275A1 (en) * 2002-09-27 2004-04-08 Dsm Ip Assets B.V. Production of zeaxanthin by phaffia
EP1546314A1 (en) * 2002-09-27 2005-06-29 DSM IP Assets B.V. Bhyd gene
NO319624B1 (no) 2003-09-15 2005-09-05 Trouw Internat Bv Fiskefôr for laksefisk i ferskvann og anvendelse av slikt fôr.
WO2006102342A2 (en) 2005-03-18 2006-09-28 Microbia, Inc. Production of carotenoids in oleaginous yeast and fungi
EP2078092A2 (en) 2006-09-28 2009-07-15 Microbia, Inc. Production of carotenoids in oleaginous yeast and fungi

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK199887D0 (da) * 1987-04-15 1987-04-15 Danisco Bioteknologi As Gaerstamme
CA2047000A1 (en) * 1990-07-20 1992-01-21 John W. Chapman Astaxanthin-generating yeast cells
US5212088A (en) * 1990-10-23 1993-05-18 Phillips Petroleum Company Spheroplast fusions of phaffia rhodozyma cells
JPH04330288A (ja) * 1991-04-11 1992-11-18 Lion Corp 微生物の育種方法

Also Published As

Publication number Publication date
JPH07501225A (ja) 1995-02-09
US5840528A (en) 1998-11-24
CA2105957A1 (en) 1994-03-12
NZ248628A (en) 1996-02-27
DE69332514D1 (de) 2003-01-09
NO933250L (no) 1994-03-14
ATE228567T1 (de) 2002-12-15
DE69332514T2 (de) 2003-09-25
WO1994006918A3 (en) 1994-07-07
AU673847B2 (en) 1996-11-28
EP0590707B1 (en) 2002-11-27
NO933250D0 (no) 1993-09-10
WO1994006918A2 (en) 1994-03-31
CA2105957C (en) 2007-04-24
DK0590707T3 (da) 2003-03-17
FI933993A0 (fi) 1993-09-10
NO313840B1 (no) 2002-12-09
FI933993A (fi) 1994-03-12
EP0590707A1 (en) 1994-04-06
AU4624293A (en) 1994-03-17

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