FI112091B - Genom DNA-rekombinantteknik konstruerade mikroorganismer som producerar amylaser och deras användning vid jäsningsprocesser - Google Patents

Genom DNA-rekombinantteknik konstruerade mikroorganismer som producerar amylaser och deras användning vid jäsningsprocesser Download PDF

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FI112091B
FI112091B FI873637A FI873637A FI112091B FI 112091 B FI112091 B FI 112091B FI 873637 A FI873637 A FI 873637A FI 873637 A FI873637 A FI 873637A FI 112091 B FI112091 B FI 112091B
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amylase
yeast
schwanniomyces
dna
glucoamylase
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FI873637A
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English (en)
Finnish (fi)
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FI873637A (fi
FI873637A0 (fi
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Alexander Strasser
Juergen Dohmen
Cornelius P Hollenberg
Feodor Bernard Martens
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Heineken Tech Services
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2414—Alpha-amylase (3.2.1.1.)
    • C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source
    • C12N9/242—Fungal source
    • A—HUMAN NECESSITIES
    • A21—BAKING; EDIBLE DOUGHS
    • A21D—TREATMENT, e.g. PRESERVATION, OF FLOUR OR DOUGH, e.g. BY ADDITION OF MATERIALS; BAKING; BAKERY PRODUCTS; PRESERVATION THEREOF
    • A21D8/00—Methods for preparing or baking dough
    • A21D8/02—Methods for preparing dough; Treating dough prior to baking
    • A21D8/04—Methods for preparing dough; Treating dough prior to baking treating dough with microorganisms or enzymes
    • A21D8/047—Methods for preparing dough; Treating dough prior to baking treating dough with microorganisms or enzymes with yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12C—BEER; PREPARATION OF BEER BY FERMENTATION; PREPARATION OF MALT FOR MAKING BEER; PREPARATION OF HOPS FOR MAKING BEER
    • C12C12/00—Processes specially adapted for making special kinds of beer
    • C12C12/002—Processes specially adapted for making special kinds of beer using special microorganisms
    • C12C12/004—Genetically modified microorganisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12C—BEER; PREPARATION OF BEER BY FERMENTATION; PREPARATION OF MALT FOR MAKING BEER; PREPARATION OF HOPS FOR MAKING BEER
    • C12C12/00—Processes specially adapted for making special kinds of beer
    • C12C12/002—Processes specially adapted for making special kinds of beer using special microorganisms
    • C12C12/006—Yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12C—BEER; PREPARATION OF BEER BY FERMENTATION; PREPARATION OF MALT FOR MAKING BEER; PREPARATION OF HOPS FOR MAKING BEER
    • C12C12/00—Processes specially adapted for making special kinds of beer
    • C12C12/02—Beer with low calorie content
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2428—Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

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  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Mycology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Distillation Of Fermentation Liquor, Processing Of Alcohols, Vinegar And Beer (AREA)
  • Bakery Products And Manufacturing Methods Therefor (AREA)

Claims (20)

1. Jästcell som former uttrycka termolabila amylo-lytiska enzym som ett resultat av att den medelst rekom- 5 binant-DNA-teknologi erhallit DNA-sekvenser fran en donor- jästcell, kännetecknad av att jästcellen förmar uttrycka Schwanniomyces οί-amylas och glukoamylas som ett resultat av att den erhällit DNA-sekvenser fran en Schwannio-myces-donorjäst, vilka DNA-sekvenser omfattar sekvenser som 10 kodar nämnda a-amylas och glukoamylas.
2. Jästcell enligt patentkrav 2, kännetecknad av att den valts bland släktena Saccharomyces, Schizo-saccharomyces, Schwanniomyces och Kluyveromyces.
3. Jästcell enligt patentkrav 2, kännetecknad 15 av att den är Saccharomyces cerevisiae.
4. Jästcell enligt patentkrav 2, kännetecknad av att den är Schwanniomyces alluvius.
5. Jästcell enligt nägot av patentkraven 1-4, kännetecknad av att donor j ästen är Schwanniomyces 20 castellii.
6. Jästcell enligt patentkrav 5, kännetecknad *· av att donorjästen är Schwanniomyces castellii ATCC 26076.
7. Jästcell enligt nägot av patentkraven 1-6, kännetecknad av att a-amylaset som uttrycks av cellen 25 har ett temperaturoptimum pä ca 37 °C och inaktiveras vid ;·, ca 60 °C. (-t·,
8. Jästcell enligt nägot av patentkraven 1-7, kännetecknad av att α-amylaset som uttrycks av cellen . kodas av DNA-sekvensen som presenteras i figur 2 eller en 30 modifikation av denna, som bibehäller funktionen hos det kodade a-amylaset.
: : : 9. Jästcell enligt nägot av patentkraven 1-8, kännetecknad av att glukoamylaset som uttrycks av . cellen har ett temperaturoptimum pä ca 50 °C, inaktiveras , 35 vid ca 60 °C och förmär hydrolysera kornstärkelse fullstän- » y digt tili glukos. 68 112091
10. Jästcell enligt nägot av patentkraven 1-9, kännetecknad av att glukoamylaset som uttrycks av cellen kodas av DNA-sekvensen som presenteras i figur 17 eller en modifikation av denna, som bibehäller funktionen 5 hos det kodade glukoamylaset.
11. Jästcell som förmär uttrycka ett termolabilt amylolytiskt enzym som ett resultat av att den medelst rekombinant-DNA-teknologi erhällit en DNA-sekvens frän en donorjäst, kännetecknad av att jästcellen förmär ut- 10 trycka Schwanniomyces α-amylas som ett resultat av att den erhällit en DNA-sekvens frän en Schwanniomyces-donorjäst, vilken DNA-sekvens omfattar en nukleotidsekvens som kodar nämnda α-amylas vilken har den aminosyrasekvens som presenteras i figur 2 eller en modifikation av denna, som bibe- 15 häller funktionen hos det kodade α-amylaset.
12. Jästcell enligt nägot av patentkraven 1 - 11, kännetecknad av att den är användbar inom livsmedels-industrin och/eller i fermenteringsprocesser, säsom en bryggeriprocess för framställning av öl, en process för 20 framställning av specialöl vilken omfattar fermentering av dextriner, bageriprocesser, eller processer för framställ- : ’ ning av etanol genom fermentering av stärkelse.
: » ; • ' · 13. Rekombinant-DNA , kännetecknad av att den : : ; omfattar de sekvenser som kodar Schwanniomyces α-amylas och •’•h 25 glukoamylas. j*.__
14. Rekombinant-DNA, kännetecknad av att den omfattar en DNA-sekvens som presenteras i figur 2 och kodar ett Schwanniomyces α-amylas, vilkens aminosyrasekvens ocksä . presenteras i figur 2. 30
15. Förfarande för f ramställning av ett termolabilt α-amylas, kännetecknat av att en jästcell enligt ;Y: nägot av patentkraven 1-12 odlas i ett lämpligt medium i » och att a-amylaset tillvaratas frän nämnda medium.
’ , 16. Förfarande för att utföra en fermenteringspro- im 35 cess som använder stärkelse som utgängsmaterial, kännetecknat av att en jästcell enligt nägot av patentkraven 69 112091 1-12 används i fermenteringsprocessen eller i förfarandet enligt patentkrav 15 för framställning av a-amylas som sedan tillvaratas och används i fermen-teringsprocessen.
17. Förfarande för framställning av biomassa, 5 kännetecknat av att man använder en fermenterings- process enligt patentkrav 16.
18. Förfarande för framställning av öl, kännetecknat av att man använder en fermenteringsprocess enligt patentkrav 16. 10
19. Förfarande för framställning av bröd, känne tecknat av att man använder en fermenteringsprocess enligt patentkrav 16.
20. Förfarande för framställning av etanol, kännetecknat av att man använder en fermenteringsprocess 15 enligt patentkrav 16. • · • * i
FI873637A 1986-08-21 1987-08-21 Genom DNA-rekombinantteknik konstruerade mikroorganismer som producerar amylaser och deras användning vid jäsningsprocesser FI112091B (sv)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
EP86111586A EP0257115A1 (en) 1986-08-21 1986-08-21 Amylolytic enzyme producing microorganisms, constructed by recombinant DNA technology, and their use in fermentation processes
EP86111586 1986-08-21
EP87110370 1987-07-17
EP87110370A EP0260404B1 (en) 1986-08-21 1987-07-17 Amylolytic enzymes producing microorganisms constructed by recombinant DNA technology and their use for fermentation processes

Publications (3)

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FI873637A0 FI873637A0 (fi) 1987-08-21
FI873637A FI873637A (fi) 1988-02-22
FI112091B true FI112091B (sv) 2003-10-31

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EP (1) EP0260404B1 (sv)
JP (3) JP2873003B2 (sv)
AU (1) AU621810B2 (sv)
CA (1) CA1340101C (sv)
DK (1) DK434987A (sv)
FI (1) FI112091B (sv)
IE (1) IE69658B1 (sv)
NO (1) NO304192B1 (sv)
NZ (1) NZ221529A (sv)
PT (1) PT85565B (sv)

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* Cited by examiner, † Cited by third party
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US5151354A (en) * 1986-08-21 1992-09-29 Cornelius Hollenberg Fermentation processes using amylolytic enzyme producing microorganisms
ATE144281T1 (de) * 1989-04-28 1996-11-15 Rhein Biotech Proz & Prod Gmbh Hefezellen der gattung-schwanniomyces
US5665585A (en) * 1992-09-03 1997-09-09 Alko-Yhiot Oy Recombinant production of glucoamylase P in trichoderma
DE4425058C2 (de) * 1994-07-15 1999-02-18 Inst Pflanzengenetik & Kultur Verfahren zur Herstellung einer thermostabilen Glukoamylase
GB9526613D0 (en) * 1995-12-28 1996-02-28 Scottish Crop Research Inst Sequence
US6900305B2 (en) 2000-08-04 2005-05-31 Battelle Memorial Institute Isolated yeast promoter sequence and a method of regulated heterologous expression
US20070248718A1 (en) * 2004-05-21 2007-10-25 Atsuki Kawamura Process for Producing Effervescent Brewage Using None of Barley, Wheat and Malt and Effervescent Brewage Produced by the Process
JP4690959B2 (ja) * 2005-07-25 2011-06-01 サントリーホールディングス株式会社 加水分取酵母を用いた発酵飲料の製造方法
JP5141133B2 (ja) * 2007-08-10 2013-02-13 東レ株式会社 連続発酵によるタンパク質の製造方法
CN103060216B (zh) 2008-05-06 2016-07-06 阿彻丹尼尔斯米德兰德公司 通过淀粉和木聚糖的降解酶的表达开发能进行淀粉和木聚糖的醇发酵的耐热酵母多形汉逊酵母菌株
JP5303046B2 (ja) * 2012-02-29 2013-10-02 サントリーホールディングス株式会社 良好な醸造香を有する低糖質発酵飲料の製造方法
JP6170206B1 (ja) * 2016-05-26 2017-07-26 アサヒビール株式会社 ビールテイスト飲料及びその製造方法
JP7206031B2 (ja) * 2016-07-12 2023-01-17 サッポロビール株式会社 ビールテイスト飲料の香味向上方法
US11274310B2 (en) 2017-09-29 2022-03-15 Dsm Ip Assets B.V. Yeast cells for glycerol free ethanol production
KR102529251B1 (ko) * 2022-10-21 2023-05-12 주식회사 바이오크래프트 향미관련 관능성이 우수한 고에스터 함유 수제 맥주 제조에 활용 가능한 신규한 토종 발효 효모 사카로마이세스 세레비지애 bc-4900

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EP0096430B2 (en) * 1982-05-19 1998-04-01 Gist-Brocades N.V. Cloning system for Kluyveromyces species
NO840200L (no) * 1983-01-28 1984-07-30 Cefus Corp Glukoamylase cdna.
CA1203765A (en) * 1983-05-11 1986-04-29 Alexander M. Sills Schwanniomyces castellii strains and brewing process
IL75210A0 (en) * 1984-05-22 1985-09-29 Bioteknika International Yeast vector
JPS60262593A (ja) * 1984-06-08 1985-12-25 Mitsui Toatsu Chem Inc 酵母グルコアミラ−ゼ遺伝子
IL76190A0 (en) * 1984-08-31 1985-12-31 Smithkline Beckman Corp Recombinant saccharomyces
JPS61141890A (ja) * 1984-12-15 1986-06-28 Suntory Ltd アルコ−ルの製造法
GB8432483D0 (en) * 1984-12-21 1985-02-06 Tubb R S Enzymes
WO1986007091A1 (en) * 1985-05-21 1986-12-04 Biotechnica International, Inc. Yeast expressing glucoamylase

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Publication number Publication date
AU7685087A (en) 1988-06-16
FI873637A (fi) 1988-02-22
JPS63133984A (ja) 1988-06-06
JP2000308493A (ja) 2000-11-07
IE872233L (en) 1988-02-21
FI873637A0 (fi) 1987-08-21
NO304192B1 (no) 1998-11-09
EP0260404B1 (en) 1995-05-31
JP3065987B2 (ja) 2000-07-17
PT85565B (pt) 1990-05-31
NO873525L (no) 1988-02-22
CA1340101C (en) 1998-10-27
PT85565A (en) 1987-09-01
DK434987D0 (da) 1987-08-20
EP0260404A2 (en) 1988-03-23
JP2873003B2 (ja) 1999-03-24
NO873525D0 (no) 1987-08-20
JPH1118783A (ja) 1999-01-26
EP0260404A3 (en) 1988-07-06
NZ221529A (en) 1992-11-25
IE69658B1 (en) 1996-10-02
AU621810B2 (en) 1992-03-26
DK434987A (da) 1988-02-22
JP3162043B2 (ja) 2001-04-25

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