FI107803B - Förfarande för producering asialoglykoproteiner av hepatit-C-virus - Google Patents
Förfarande för producering asialoglykoproteiner av hepatit-C-virus Download PDFInfo
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- FI107803B FI107803B FI932025A FI932025A FI107803B FI 107803 B FI107803 B FI 107803B FI 932025 A FI932025 A FI 932025A FI 932025 A FI932025 A FI 932025A FI 107803 B FI107803 B FI 107803B
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- hcv
- mannose
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- A—HUMAN NECESSITIES
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
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- C12N2770/24211—Hepacivirus, e.g. hepatitis C virus, hepatitis G virus
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Claims (8)
1. Menetelmä HCV-proteiinien tuottamiseksi, mitkä ovat sopivia käytettäväksi rokotteessa tai immunologisessa analyysissä, 5 mikä menetelmä käsittää sen, että: kasvatetaan nisäkäsisäntäsolua, mikä on transformoitu HCV-proteiinia koodaavalla rakennegeenillä, sopivassa viljely-väliaineessa; aiheutetaan rakennegeenin ilmentyminen; ja ίο eristetään HCV-asialoglykoproteiini soluviljelmästä tunnettu siitä että mainittu HCV-proteiini on asialoglykoproteiini, johon N-liitetystä hiilihydraatista vähemmän kuin noin 10 % on sialihappoa; asialoglykoproteiini on valittu ryhmästä mikä käsittää El:n ja E2:n ja näiden is aggregaatit; ilmentyminen tapahtuu sialylaatiota estävissä olosuhteissa, ja eristys tapahtuu saattamalla soluviljelmä kosketukseen mannoosia sitovan proteiinin kanssa joka proteiini on mannoosipäätteisille glykoproteiineille spesifinen sekä eristämällä mannoosia sitovaan proteiiniin 20 sitoutuva soluviljelmän osa. ♦ · * · · ·«· · : 2. Patenttivaatimuksen 1 mukainen menetelmä, tunnettu siitä, e «e ♦ · »***. että sialylaatiota estävät olosuhteet käsittävät «·· ·1·,· asialoglykoproteiinin ilmentämisen nopeudella, joka on • · ·2·1: 25 riittävä estämään glykoproteiinien kuljettamisen • e ·2·1: endoplasmaattisesta verkostosta golgin laitteeseen. • *:·2: 3. Patenttivaatimuksen 1 mukainen menetelmä, tunnettu siitä, että sialylaatiota estävät olosuhteet käsittävät riittävän : 30 määrän kalsiummodulaattoria aiheuttamaan proteiinien • · · e·· · .***. vapautumisen isäntäsolun endoplasmaattisessa verkostossa. • 1· . 4. Patenttivaatimuksen 3 mukainen menetelmä, tunnettu siitä, 2 • · että kalsiummodulaattori on valittu ryhmästä joka koostuu 107803 tapsigariinista ja EGTArsta (etyleeniglykolibis[beeta-aminoetyylieetteri]N, N, Ν', N'-tetraetikkahappo).
5. Jonkin patenttivaatimuksista 1-4 mukainen menetelmä, 5 tunnettu siitä, että mannoosia sitova proteiini on lektiini.
6. Patenttivaatimuksen 5 mukainen menetelmä, tunnettu siitä, että mannoosia sitova proteiini on GNA. ίο 7. Jonkin patenttivaatimuksista 1-6 mukainen menetelmä, tunnettu siitä, että mannoosia sitova proteiini on kiinnitettynä kantajaan.
8. Jonkin patenttivaatimuksista 1-7 mukainen menetelmä, 15 tunnettu siitä, että saattaminen kosketukseen käsittää HCV-asialoglykoproteiinit sisältävän soluviljelmän inkuboinnin kolonnissa, joka käsittää mannoosia sitovaa proteiinia kiinnitettynä kantajaan, ainakin tunnin ajan, ja että asialoglykoproteiini eristetään uuttamalla sitoutunut osa 20 mannoosiliuoksella. .·. Patentkrav • · · I * · • · · • · · • · J”; 1. Förfarande för producering av HCV-proteiner, vilka är 1 2 3 4 5 6 • · lämpliga för användning i en vaccin eller i en immunologisk 2 • · 3 :*·*: analys, vilket förfarande omfattar, att man 4 odlar en däggdjursvärdcell som är transformerad med en 5 strukturgen, som kodar för HCV-proteinet, i ett lämpligt ·:**: odlingsmedium; 6 förorsakar expression av strukturgenen; ooh j isolerar HCV-asialoglykoproteinet fran cellodlingen, IM · .**·. kännetecknat därav, att nämnda HCV-protein är ett IM * . asialoglykoprotein, tili vilket är N-bundet ett kolhydrat av • · .vilket mindre än cirka 10 % är sialsyra; asialoglykoproteinet 107803 är valt ur gruppen som omfattar El och E2 och aggregat av dessa; expressionen sker under förhällanden som förhindrar sialylering, och isoleringen sker genom att försätta cellodlingen i kontakt med ett mannosbindande protein, vilket 5 protein är specifikt för glykoproteiner med mannosända, samt genom att isolera den del av cellodlingen som binder sig tili det mannosbindande proteinet.
2. Förfarande enligt patentkravet 1, kännetecknat därav, att ίο de förhällanden som förhindrar sialylering omfattar expression av asialoglykoproteinet med en hastighet som är tillräcklig för att hindra transport av glykoproteiner frän det endoplasmatiska nätverket till Golgikoraplexet. is 3. Förfarande enligt patentkravet 1, kännetecknat därav, att de sialylering förhindrande förhällandena omfattar en tillräcklig mängd av en kalsiummodulator för att förorsaka frigörelsen av proteiner i det endoplasmatiska nätverket av värdcellen. 20
4. Förfarande enligt patentkravet 3, kännetecknat därav, att . kalsiummodulatorn är vald ur gruppen som bestär av tapsigarin ··» · och EGTA (etylenglykolbis [betaaminoetyleter] N, N, Ν', N'- :***: tetraättiksyra) . : 25 • · • · · j 1 : 5. Förfarande enligt nägot av patentkraven 1-4, kännetecknat ··· !.· · därav, att det mannosbindande proteinet är lektin. , 6. Förfarande enligt patentkravet 5, kännetecknat därav, att • · · 30 det mannosbindande proteinet är GNA. « ► · · • · · • · ♦ ··« ·
7. Förfarande enligt nägot av patentkraven 1-6, kännetecknat • · ♦ därav, att det mannnosbindande proteinet är bundet tili en • · • · bärare. 107803
8. Förfarande enligt nägot av patentkraven 1-7, kännetecknat därav, att försättningen i kontakt med omfattar, att cellodlingen, som innehäller HCV-asialoglykoproteiner, s inkuberas i en kolonn, som omfattar ett mannosbindande protein bundet tili en bärare, i ätminstone en timme, och att asialoglykoproteinet isoleras genom att extrahera den bundna delen med en mannoslösning. # · · M» · * · • · · • »· • · • · · • · » · • · · • · • · · • ·# » · • I · • · · • · • · • · · « · · «tl (··!» • · • * · • · «I» • · • » · • · · « · · · • · « • · • · • · · « · · « · • * *··!· • ·
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| FI971702A FI107804B (sv) | 1990-11-08 | 1997-04-21 | Asialoglykoproteiner av hepatit-C-virus som används i immunobestämningar |
Applications Claiming Priority (8)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US61196590A | 1990-11-08 | 1990-11-08 | |
| US61141990A | 1990-11-08 | 1990-11-08 | |
| US61196590 | 1990-11-08 | ||
| US61141990 | 1990-11-08 | ||
| US75888091A | 1991-09-13 | 1991-09-13 | |
| US75888091 | 1991-09-13 | ||
| PCT/US1991/008272 WO1992008734A1 (en) | 1990-11-08 | 1991-11-07 | Hepatitis c virus asialoglycoproteins |
| US9108272 | 1991-11-07 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| FI932025A0 FI932025A0 (fi) | 1993-05-05 |
| FI932025A FI932025A (fi) | 1993-06-07 |
| FI107803B true FI107803B (sv) | 2001-10-15 |
Family
ID=27417050
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| FI932025A FI107803B (sv) | 1990-11-08 | 1993-05-05 | Förfarande för producering asialoglykoproteiner av hepatit-C-virus |
Country Status (17)
| Country | Link |
|---|---|
| EP (2) | EP0556292B2 (sv) |
| JP (8) | JPH06504431A (sv) |
| AT (1) | ATE188220T1 (sv) |
| AU (1) | AU668078B2 (sv) |
| CA (2) | CA2203443C (sv) |
| CZ (1) | CZ289006B6 (sv) |
| DE (1) | DE69131882T3 (sv) |
| DK (2) | DK0842947T4 (sv) |
| ES (1) | ES2139591T5 (sv) |
| FI (1) | FI107803B (sv) |
| GR (1) | GR3032771T3 (sv) |
| HU (2) | HUT66063A (sv) |
| NO (2) | NO304380B1 (sv) |
| PT (2) | PT99466B (sv) |
| RO (1) | RO115446B1 (sv) |
| SK (3) | SK285624B6 (sv) |
| WO (1) | WO1992008734A1 (sv) |
Families Citing this family (32)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6274148B1 (en) | 1990-11-08 | 2001-08-14 | Chiron Corporation | Hepatitis C virus asialoglycoproteins |
| JP3158177B2 (ja) * | 1991-10-08 | 2001-04-23 | 国立感染症研究所長 | Ｃ型肝炎診断薬 |
| AU666176B2 (en) * | 1992-01-31 | 1996-02-01 | Abbott Laboratories | Mammalian expression systems for HCV proteins |
| UA39944C2 (uk) * | 1992-07-07 | 2001-07-16 | Чірон Корпорейшн | Спосіб визначення ранньої сероконверсії у ссавця-хазяїна до вірусу гепатиту с і набір для використання в способі |
| EP1421951A3 (en) | 1993-05-12 | 2005-10-05 | Chiron Corporation | Conserved motif of hepatitis C virus E2/NS1 region |
| US5514539A (en) * | 1993-06-29 | 1996-05-07 | The United States Of America As Represented By The Department Of Health And Human Services | Nucleotide and deduced amino acid sequences of the envelope 1 gene of 51 isolates of hepatitis C virus and the use of reagents derived from these sequences in diagnostic methods and vaccines |
| US5610009A (en) * | 1994-01-28 | 1997-03-11 | Abbott Laboratories | Mammalian expression systems for hepatitis C virus envelope genes |
| PT773957E (pt) * | 1994-07-29 | 2005-11-30 | Chiron Corp | Polipeptidos truncados e1 e e2 de hepatite c inovadores, e metodos de obtencao dos mesmos |
| ES2316920T3 (es) | 1994-07-29 | 2009-04-16 | Novartis Vaccines And Diagnostics, Inc. | Popipeptido truncado e2 de hepatitis c y procedimientos para obtener el mismo. |
| CA2172273A1 (en) * | 1994-07-29 | 1996-02-15 | Geert Maertens | Purified hepatitis c virus envelope proteins for diagnostic and therapeutic use |
| IT1271593B (it) * | 1994-11-30 | 1997-06-04 | Sanitaria Scaligera Spa | Procedimento ed apparecchiatura per l'immunoadsorbimento specifico di virus hiv, di antigene gp120 e complesso cd4-gp120. |
| US6127116A (en) | 1995-08-29 | 2000-10-03 | Washington University | Functional DNA clone for hepatitis C virus (HCV) and uses thereof |
| FR2744725B1 (fr) * | 1996-02-09 | 1998-04-30 | Pasteur Institut | Anticorps specifiques des complexes formes par les glycoproteines e1 et e2 du virus de l'hepatite c, procedes de determination de la presence, et d'isolement de ces complexes |
| US6514731B1 (en) | 1996-05-24 | 2003-02-04 | Chiron Corporation | Methods for the preparation of hepatitis C virus multiple copy epitope fusion antigens |
| KR100209095B1 (ko) * | 1996-06-28 | 1999-07-15 | 성재갑 | C형 간염 바이러스의 프로테아제의 활성을 측정할 수 있는 c형 간염 대체 바이러스, 그 재조합 유전자 및 그 용도 |
| AU738585B2 (en) | 1996-11-08 | 2001-09-20 | Government Of The United States Of America, As Represented By The Secretary Of The Department Of Health And Human Services, The | Synthesis and purification of hepatitis C virus-like particles |
| US7338759B1 (en) | 1997-03-04 | 2008-03-04 | Washington University | HCV variants |
| US7049428B1 (en) | 1998-03-04 | 2006-05-23 | Washington University | HCV variants |
| JP4458556B2 (ja) | 1997-05-06 | 2010-04-28 | ノバルティス バクシンズ アンド ダイアグノスティックス，インコーポレーテッド | Ｃ型肝炎のｅ２短縮型ポリペプチドの細胞内生成 |
| EP0947525A1 (en) | 1998-03-27 | 1999-10-06 | Innogenetics N.V. | Epitopes in viral envelope proteins and specific antibodies directed against these epitopes: use for detection of HCV viral antigen in host tissue |
| US7108855B2 (en) | 1998-06-24 | 2006-09-19 | Innogenetics N.V. | Purified hepatitis C virus envelope proteins for diagnostic and therapeutic use |
| WO2002014362A2 (en) | 2000-08-17 | 2002-02-21 | Tripep Ab | A hepatitis c virus non-structural ns3/4a fusion gene |
| US6680059B2 (en) | 2000-08-29 | 2004-01-20 | Tripep Ab | Vaccines containing ribavirin and methods of use thereof |
| US7022830B2 (en) | 2000-08-17 | 2006-04-04 | Tripep Ab | Hepatitis C virus codon optimized non-structural NS3/4A fusion gene |
| RU2286172C2 (ru) | 2000-08-17 | 2006-10-27 | Трипеп Аб | Вакцины, содержащие рибавирин, и способы их использования |
| US7101561B2 (en) | 2000-12-01 | 2006-09-05 | Innogenetics N.V. | Purified hepatitis C virus envelope proteins for diagnostic and therapeutic use |
| US20030232745A1 (en) * | 2001-06-26 | 2003-12-18 | Olson William C. | Uses of DC-sign and DC-Signr for inhibiting hepatitis C virus infection |
| US7022323B2 (en) | 2001-06-26 | 2006-04-04 | Progenics Pharmaceuticals, Inc. | Uses of DC-SIGN and DC-SIGNR for inhibiting hepatitis C virus infection |
| AU2003265454A1 (en) * | 2002-08-16 | 2004-03-03 | Ohio University | Hepatitis c viral-like particle purification |
| WO2007031867A2 (en) | 2005-05-25 | 2007-03-22 | Tripep Ab | A hepatitis c virus non-stru tural ns3/4a fusion gene |
| EP2185195A2 (en) | 2007-08-16 | 2010-05-19 | Tripep Ab | Immunogen platform |
| WO2009130588A2 (en) | 2008-04-22 | 2009-10-29 | Tripep Ab | Immunogen platform |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1049686C (zh) * | 1987-11-18 | 2000-02-23 | 希龙股份有限公司 | 非ａ和非ｂ肝炎病毒的诊断及疫苗 |
| IL88599A0 (en) * | 1987-12-10 | 1989-07-31 | Cell Bio Group Ltd | Glycoprotein cell growth modulating materials,their preparation and compositions containing them |
| JP2656995B2 (ja) * | 1989-03-17 | 1997-09-24 | カイロン コーポレイション | Ｎａｎｂｖの診断用薬 |
| JP2733138B2 (ja) * | 1990-04-04 | 1998-03-30 | カイロン コーポレイション | 抗ｈｃｖ抗体の免疫アッセイに使用するｃ型肝炎ウイルス（ｈｃｖ）抗原の組合せ |
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1991
- 1991-11-07 SK SK690-97A patent/SK285624B6/sk not_active IP Right Cessation
- 1991-11-07 DK DK97120661T patent/DK0842947T4/da active
- 1991-11-07 EP EP92900091A patent/EP0556292B2/en not_active Expired - Lifetime
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- 1991-11-07 SK SK442-93A patent/SK285623B6/sk not_active IP Right Cessation
- 1991-11-07 AT AT92900091T patent/ATE188220T1/de not_active IP Right Cessation
- 1991-11-07 WO PCT/US1991/008272 patent/WO1992008734A1/en active IP Right Grant
- 1991-11-07 RO RO93-00626A patent/RO115446B1/ro unknown
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1993
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- 1993-05-07 NO NO931680A patent/NO304380B1/no not_active IP Right Cessation
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1997
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1998
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2000
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2001
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2002
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2005
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2006
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| PC | Transfer of assignment of patent |
Owner name: NOVARTIS VACCINES AND DIAGNOSTICS, INC. Free format text: NOVARTIS VACCINES AND DIAGNOSTICS, INC. |
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| MA | Patent expired |