FI106720B - Fuusioproteiinien valmistus in vitro - Google Patents

Fuusioproteiinien valmistus in vitro Download PDF

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Publication number
FI106720B
FI106720B FI913405A FI913405A FI106720B FI 106720 B FI106720 B FI 106720B FI 913405 A FI913405 A FI 913405A FI 913405 A FI913405 A FI 913405A FI 106720 B FI106720 B FI 106720B
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FI
Finland
Prior art keywords
protein
amino acid
lys
ser
asp
Prior art date
Application number
FI913405A
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English (en)
Finnish (fi)
Swedish (sv)
Other versions
FI913405A (fi
FI913405A0 (fi
Inventor
Jutta Heim
Kenji Takabayashi
Peter Seeboth
Original Assignee
Novartis Ag
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Publication date
Application filed by Novartis Ag filed Critical Novartis Ag
Publication of FI913405A0 publication Critical patent/FI913405A0/fi
Publication of FI913405A publication Critical patent/FI913405A/fi
Application granted granted Critical
Publication of FI106720B publication Critical patent/FI106720B/fi

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00Preparation of peptides or proteins
    • C12P21/06Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575Hormones
    • C07K14/585Calcitonins
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575Hormones
    • C07K14/65Insulin-like growth factors, i.e. somatomedins, e.g. IGF-1, IGF-2
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/81Protease inhibitors
    • C07K14/815Protease inhibitors from leeches, e.g. hirudin, eglin
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62DNA sequences coding for fusion proteins
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/48Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/58Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi
    • C12N9/60Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi from yeast
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • C07K2319/01Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • C07K2319/20Fusion polypeptide containing a tag with affinity for a non-protein ligand
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • C07K2319/50Fusion polypeptide containing protease site
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • C07K2319/70Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/74Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
    • C07K2319/75Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Microbiology (AREA)
  • Endocrinology (AREA)
  • Toxicology (AREA)
  • Mycology (AREA)
  • Diabetes (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
FI913405A 1990-07-18 1991-07-15 Fuusioproteiinien valmistus in vitro FI106720B (fi)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB909015825A GB9015825D0 (en) 1990-07-18 1990-07-18 In vitro processing of fusion proteins
GB9015825 1990-07-18

Publications (3)

Publication Number Publication Date
FI913405A0 FI913405A0 (fi) 1991-07-15
FI913405A FI913405A (fi) 1992-01-19
FI106720B true FI106720B (fi) 2001-03-30

Family

ID=10679295

Family Applications (1)

Application Number Title Priority Date Filing Date
FI913405A FI106720B (fi) 1990-07-18 1991-07-15 Fuusioproteiinien valmistus in vitro

Country Status (19)

Country Link
EP (1) EP0467839B1 (no)
JP (1) JP3140488B2 (no)
AT (1) ATE141645T1 (no)
AU (1) AU651830B2 (no)
CA (1) CA2047119C (no)
DE (1) DE69121489T2 (no)
DK (1) DK0467839T3 (no)
ES (1) ES2091897T3 (no)
FI (1) FI106720B (no)
GB (1) GB9015825D0 (no)
GR (1) GR3020840T3 (no)
HU (1) HU216104B (no)
IE (1) IE73209B1 (no)
NO (1) NO303132B1 (no)
NZ (1) NZ238989A (no)
PT (1) PT98332B (no)
SG (1) SG49863A1 (no)
TW (1) TW209247B (no)
ZA (1) ZA915579B (no)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5525491A (en) * 1991-02-27 1996-06-11 Creative Biomolecules, Inc. Serine-rich peptide linkers
CA2213512A1 (en) * 1995-12-22 1997-07-03 Toray Industries, Inc. Method for producing bioactive fusion protein
US6800726B1 (en) 1996-11-01 2004-10-05 Pioneer Hi-Bred International, Inc. Proteins with increased levels of essential amino acids
US6265204B1 (en) 1997-01-17 2001-07-24 Genencor International, Inc. DNA sequences, vectors, and fusion polypeptides for secretion of polypeptides in filamentous fungi
GB9716790D0 (en) 1997-08-07 1997-10-15 Creative Peptides Sweden Ab Recombinant DNA molecules comprising multimeric copies of a gene sequence and expression thereof
ES2843679T3 (es) 2013-03-06 2021-07-20 Glaxosmithkline Llc Células hospedadoras y métodos de uso

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
MC1834A1 (fr) * 1986-07-10 1988-06-03 Transgene Sa Bloc fonctionnel d'adn et plasmide codant pour l'hirudine,levure transformee,procede de preparation de l'hirudine,hirudine obtenue et son utilisation
US5010182A (en) * 1987-07-28 1991-04-23 Chiron Corporation DNA constructs containing a Kluyveromyces alpha factor leader sequence for directing secretion of heterologous polypeptides
DK105489D0 (da) * 1989-03-03 1989-03-03 Novo Nordisk As Polypeptid

Also Published As

Publication number Publication date
AU8046391A (en) 1992-01-23
PT98332B (pt) 1999-01-29
DE69121489D1 (de) 1996-09-26
ATE141645T1 (de) 1996-09-15
IE912501A1 (en) 1992-01-29
NZ238989A (en) 1992-09-25
FI913405A (fi) 1992-01-19
FI913405A0 (fi) 1991-07-15
CA2047119C (en) 2002-10-08
EP0467839A1 (en) 1992-01-22
JPH04229188A (ja) 1992-08-18
SG49863A1 (en) 2002-03-19
EP0467839B1 (en) 1996-08-21
ES2091897T3 (es) 1996-11-16
HU912398D0 (en) 1991-12-30
NO912803D0 (no) 1991-07-17
TW209247B (no) 1993-07-11
GB9015825D0 (en) 1990-09-05
HUT58369A (en) 1992-02-28
NO912803L (no) 1992-01-20
CA2047119A1 (en) 1992-01-19
AU651830B2 (en) 1994-08-04
JP3140488B2 (ja) 2001-03-05
IE73209B1 (en) 1997-05-07
NO303132B1 (no) 1998-06-02
PT98332A (pt) 1992-05-29
DK0467839T3 (da) 1996-09-16
DE69121489T2 (de) 1997-02-27
HU216104B (hu) 1999-04-28
ZA915579B (en) 1992-03-25
GR3020840T3 (en) 1996-11-30

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Owner name: NOVARTIS AG