FI106241B - Biomassa virus/virusantigeenin tuottamiseksi - Google Patents
Biomassa virus/virusantigeenin tuottamiseksi Download PDFInfo
- Publication number
- FI106241B FI106241B FI923099A FI923099A FI106241B FI 106241 B FI106241 B FI 106241B FI 923099 A FI923099 A FI 923099A FI 923099 A FI923099 A FI 923099A FI 106241 B FI106241 B FI 106241B
- Authority
- FI
- Finland
- Prior art keywords
- virus
- biomass
- cell
- mmol
- antigen
- Prior art date
Links
- 241000700605 Viruses Species 0.000 title claims description 54
- 239000000427 antigen Substances 0.000 title claims description 46
- 102000036639 antigens Human genes 0.000 title claims description 46
- 108091007433 antigens Proteins 0.000 title claims description 46
- 239000002028 Biomass Substances 0.000 title claims description 34
- 210000004027 cell Anatomy 0.000 claims description 52
- 238000004519 manufacturing process Methods 0.000 claims description 23
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 16
- 239000001301 oxygen Substances 0.000 claims description 16
- 229910052760 oxygen Inorganic materials 0.000 claims description 16
- 238000000034 method Methods 0.000 claims description 14
- 239000001963 growth medium Substances 0.000 claims description 10
- 241000710771 Tick-borne encephalitis virus Species 0.000 claims description 5
- 238000012546 transfer Methods 0.000 claims description 5
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 4
- 241001465754 Metazoa Species 0.000 claims description 4
- 210000002308 embryonic cell Anatomy 0.000 claims description 4
- 239000008103 glucose Substances 0.000 claims description 4
- 230000002503 metabolic effect Effects 0.000 claims description 4
- 241000287828 Gallus gallus Species 0.000 claims description 3
- 206010014599 encephalitis Diseases 0.000 claims description 3
- 241000700663 Avipoxvirus Species 0.000 claims description 2
- 241000700618 Vaccinia virus Species 0.000 claims description 2
- 206010046865 Vaccinia virus infection Diseases 0.000 claims description 2
- 230000004931 aggregating effect Effects 0.000 claims description 2
- 239000003795 chemical substances by application Substances 0.000 claims description 2
- 206010022000 influenza Diseases 0.000 claims description 2
- 201000011475 meningoencephalitis Diseases 0.000 claims description 2
- 241000712461 unidentified influenza virus Species 0.000 claims description 2
- 208000007089 vaccinia Diseases 0.000 claims description 2
- 239000000758 substrate Substances 0.000 claims 3
- 230000000712 assembly Effects 0.000 claims 1
- 238000000429 assembly Methods 0.000 claims 1
- 210000005167 vascular cell Anatomy 0.000 claims 1
- 230000003612 virological effect Effects 0.000 description 37
- 230000001413 cellular effect Effects 0.000 description 12
- 239000000725 suspension Substances 0.000 description 8
- 238000004062 sedimentation Methods 0.000 description 6
- 238000004113 cell culture Methods 0.000 description 5
- 230000000694 effects Effects 0.000 description 5
- 238000002156 mixing Methods 0.000 description 5
- 238000002360 preparation method Methods 0.000 description 5
- 108090000623 proteins and genes Proteins 0.000 description 4
- 102000004169 proteins and genes Human genes 0.000 description 4
- 210000003754 fetus Anatomy 0.000 description 3
- 241000271566 Aves Species 0.000 description 2
- 238000002965 ELISA Methods 0.000 description 2
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 description 2
- 108091005804 Peptidases Proteins 0.000 description 2
- 210000004556 brain Anatomy 0.000 description 2
- 238000011109 contamination Methods 0.000 description 2
- 239000011148 porous material Substances 0.000 description 2
- 238000012216 screening Methods 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 101150096316 5 gene Proteins 0.000 description 1
- LHEJDBBHZGISGW-UHFFFAOYSA-N 5-fluoro-3-(3-oxo-1h-2-benzofuran-1-yl)-1h-pyrimidine-2,4-dione Chemical compound O=C1C(F)=CNC(=O)N1C1C2=CC=CC=C2C(=O)O1 LHEJDBBHZGISGW-UHFFFAOYSA-N 0.000 description 1
- 101710186708 Agglutinin Proteins 0.000 description 1
- 108090000317 Chymotrypsin Proteins 0.000 description 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
- 101710146024 Horcolin Proteins 0.000 description 1
- 101710189395 Lectin Proteins 0.000 description 1
- 101710179758 Mannose-specific lectin Proteins 0.000 description 1
- 101710150763 Mannose-specific lectin 1 Proteins 0.000 description 1
- 101710150745 Mannose-specific lectin 2 Proteins 0.000 description 1
- 108010067372 Pancreatic elastase Proteins 0.000 description 1
- 102000016387 Pancreatic elastase Human genes 0.000 description 1
- 102000035195 Peptidases Human genes 0.000 description 1
- 239000004365 Protease Substances 0.000 description 1
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 1
- 108090000631 Trypsin Proteins 0.000 description 1
- 102000004142 Trypsin Human genes 0.000 description 1
- 239000000910 agglutinin Substances 0.000 description 1
- 210000004102 animal cell Anatomy 0.000 description 1
- VEZXCJBBBCKRPI-UHFFFAOYSA-N beta-propiolactone Chemical compound O=C1CCO1 VEZXCJBBBCKRPI-UHFFFAOYSA-N 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 239000000969 carrier Substances 0.000 description 1
- 239000006143 cell culture medium Substances 0.000 description 1
- 230000007910 cell fusion Effects 0.000 description 1
- 230000006037 cell lysis Effects 0.000 description 1
- 239000006285 cell suspension Substances 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 210000003837 chick embryo Anatomy 0.000 description 1
- 229960002376 chymotrypsin Drugs 0.000 description 1
- 239000012531 culture fluid Substances 0.000 description 1
- 239000012228 culture supernatant Substances 0.000 description 1
- 238000005520 cutting process Methods 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 230000034994 death Effects 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 235000013601 eggs Nutrition 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 230000001605 fetal effect Effects 0.000 description 1
- 230000004578 fetal growth Effects 0.000 description 1
- 239000012634 fragment Substances 0.000 description 1
- 230000004927 fusion Effects 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- 210000005260 human cell Anatomy 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 230000002779 inactivation Effects 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 229960000380 propiolactone Drugs 0.000 description 1
- 229940024999 proteolytic enzymes for treatment of wounds and ulcers Drugs 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 239000013049 sediment Substances 0.000 description 1
- 239000007858 starting material Substances 0.000 description 1
- 230000003068 static effect Effects 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 238000010257 thawing Methods 0.000 description 1
- 239000012588 trypsin Substances 0.000 description 1
- 238000000108 ultra-filtration Methods 0.000 description 1
- 229960005486 vaccine Drugs 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2770/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
- C12N2770/00011—Details
- C12N2770/24011—Flaviviridae
- C12N2770/24051—Methods of production or purification of viral material
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2770/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
- C12N2770/00011—Details
- C12N2770/24011—Flaviviridae
- C12N2770/24111—Flavivirus, e.g. yellow fever virus, dengue, JEV
- C12N2770/24151—Methods of production or purification of viral material
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Virology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Medicinal Chemistry (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Immunology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Semiconductor Memories (AREA)
- Non-Volatile Memory (AREA)
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| AT18/90A AT393277B (de) | 1990-01-04 | 1990-01-04 | Verfahren zur herstellung von fruehsommer- meningoenzephalitis-virus (fsme-virus)-antigen |
| AT1890 | 1990-01-04 | ||
| PCT/AT1991/000003 WO1991009937A1 (de) | 1990-01-04 | 1991-01-03 | Biomasse zur produktion von virus/virusantigen |
| AT9100003 | 1991-01-03 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| FI923099A0 FI923099A0 (fi) | 1992-07-03 |
| FI923099A FI923099A (fi) | 1992-07-03 |
| FI106241B true FI106241B (fi) | 2000-12-29 |
Family
ID=3479351
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| FI923099A FI106241B (fi) | 1990-01-04 | 1992-07-03 | Biomassa virus/virusantigeenin tuottamiseksi |
Country Status (17)
| Country | Link |
|---|---|
| US (2) | US5391491A (no) |
| EP (1) | EP0509008B1 (no) |
| JP (1) | JP2633392B2 (no) |
| AT (2) | AT393277B (no) |
| CA (1) | CA2073168C (no) |
| CZ (1) | CZ279725B6 (no) |
| DE (1) | DE59103696D1 (no) |
| DK (1) | DK0509008T3 (no) |
| ES (1) | ES2067921T3 (no) |
| FI (1) | FI106241B (no) |
| HR (1) | HRP921353A2 (no) |
| HU (1) | HU212597B (no) |
| NO (1) | NO310472B1 (no) |
| RU (1) | RU2099419C1 (no) |
| SK (1) | SK279200B6 (no) |
| WO (1) | WO1991009937A1 (no) |
| YU (1) | YU391A (no) |
Families Citing this family (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5719051A (en) * | 1989-12-22 | 1998-02-17 | Immuno Aktiengesellschaft | Perfusion system and a method for the large scale production of virus or virus antigen |
| AT393277B (de) * | 1990-01-04 | 1991-09-25 | Immuno Ag | Verfahren zur herstellung von fruehsommer- meningoenzephalitis-virus (fsme-virus)-antigen |
| US5753489A (en) * | 1994-11-10 | 1998-05-19 | Immuno Ag | Method for producing viruses and vaccines in serum-free culture |
| US5698433A (en) * | 1994-11-10 | 1997-12-16 | Immuno Ag | Method for producing influenza virus and vaccine |
| US5756341A (en) * | 1994-11-10 | 1998-05-26 | Immuno Ag | Method for controlling the infectivity of viruses |
| DE69535940D1 (de) | 1994-11-10 | 2009-06-04 | Baxter Healthcare Sa | Verfahren zur Herstellung von biologischen Produkten in Protein-freiem Kultur |
| US6146873A (en) * | 1994-11-10 | 2000-11-14 | Baxter Aktiengesellschaft | Production of orthomyxoviruses in monkey kidney cells using protein-free media |
| DE19612966B4 (de) | 1996-04-01 | 2009-12-10 | Novartis Vaccines And Diagnostics Gmbh & Co. Kg | MDCK-Zellen und Verfahren zur Vermehrung von Influenzaviren |
| BR9804283B1 (pt) * | 1998-03-18 | 2010-11-30 | processo para a produção de flavivìrus em baixa densidade de células em cultura e processo para a produção de flavivìrus recombinante em baixa densidade de células em cultura. | |
| US7445924B2 (en) * | 2000-11-23 | 2008-11-04 | Bavarian Nordic A/S | Modified Vaccinia Ankara virus variant and cultivation method |
| DE10144906B4 (de) | 2001-09-12 | 2013-11-28 | Novartis Vaccines And Diagnostics Gmbh | Verfahren zur großtechnischen Herstellung von Impfstoffen |
| US20040023358A1 (en) * | 2001-12-21 | 2004-02-05 | Wyeth | Avian embryo particulate biomass for the production of virus antigens |
| PT1434858E (pt) * | 2002-09-05 | 2008-07-28 | Bavarian Nordic As | Método de amplificação de um poxvírus em condições sem soro |
| US7998488B2 (en) | 2008-11-14 | 2011-08-16 | Baxter International Inc. | Vaccine formulations and uses thereof |
| AU2011305348A1 (en) | 2010-09-23 | 2013-04-18 | Baxter Healthcare Sa | Recombinant viral vectors and methods for inducing an immune response to yellow fever virus |
| CN103458922B (zh) | 2011-01-31 | 2017-10-03 | 纳米医疗公司 | 诱导对甲型流感病毒的异源亚型免疫反应的重组病毒载体和方法 |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4059485A (en) * | 1976-11-03 | 1977-11-22 | Monsanto Company | Adaptation of cell lines to suspension culture |
| US4195130A (en) * | 1978-04-20 | 1980-03-25 | Cornell Research Foundation, Inc. | Propagation of feline infectious peritonitis virus in tissue cultures |
| AT358167B (de) * | 1978-12-22 | 1980-08-25 | Immuno Ag | Verfahren zur herstellung von fruehsommer- -meningoenzephalitis-virus (fsme-virus)- -vakzinen |
| US4335215A (en) * | 1980-08-27 | 1982-06-15 | Monsanto Company | Method of growing anchorage-dependent cells |
| AT393277B (de) * | 1990-01-04 | 1991-09-25 | Immuno Ag | Verfahren zur herstellung von fruehsommer- meningoenzephalitis-virus (fsme-virus)-antigen |
| US5114855A (en) * | 1990-04-19 | 1992-05-19 | Regents Of The University Of Minnesota | Method for aggregating cells with small microspheres |
-
1990
- 1990-01-04 AT AT18/90A patent/AT393277B/de not_active IP Right Cessation
-
1991
- 1991-01-03 HU HU9202127A patent/HU212597B/hu unknown
- 1991-01-03 WO PCT/AT1991/000003 patent/WO1991009937A1/de active IP Right Grant
- 1991-01-03 EP EP19910901647 patent/EP0509008B1/de not_active Expired - Lifetime
- 1991-01-03 CA CA 2073168 patent/CA2073168C/en not_active Expired - Lifetime
- 1991-01-03 JP JP50199791A patent/JP2633392B2/ja not_active Expired - Fee Related
- 1991-01-03 RU SU925052917A patent/RU2099419C1/ru not_active IP Right Cessation
- 1991-01-03 ES ES91901647T patent/ES2067921T3/es not_active Expired - Lifetime
- 1991-01-03 US US07/854,630 patent/US5391491A/en not_active Expired - Lifetime
- 1991-01-03 YU YU391A patent/YU391A/sh unknown
- 1991-01-03 DE DE59103696T patent/DE59103696D1/de not_active Expired - Fee Related
- 1991-01-03 AT AT91901647T patent/ATE114713T1/de not_active IP Right Cessation
- 1991-01-03 DK DK91901647T patent/DK0509008T3/da active
- 1991-01-04 CZ CS9112A patent/CZ279725B6/cs not_active IP Right Cessation
- 1991-01-04 SK SK12-91A patent/SK279200B6/sk not_active IP Right Cessation
-
1992
- 1992-07-02 NO NO19922622A patent/NO310472B1/no not_active IP Right Cessation
- 1992-07-03 FI FI923099A patent/FI106241B/fi active
- 1992-11-25 HR HRP921353 patent/HRP921353A2/xx not_active Application Discontinuation
-
1994
- 1994-12-01 US US08/352,077 patent/US5550051A/en not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| HUT64583A (en) | 1994-01-28 |
| HU212597B (en) | 1996-08-29 |
| JP2633392B2 (ja) | 1997-07-23 |
| WO1991009937A1 (de) | 1991-07-11 |
| NO922622D0 (no) | 1992-07-02 |
| CZ279725B6 (cs) | 1995-06-14 |
| EP0509008A1 (de) | 1992-10-21 |
| CS9100012A2 (en) | 1991-08-13 |
| YU391A (sh) | 1994-04-05 |
| HRP921353A2 (en) | 1995-10-31 |
| RU2099419C1 (ru) | 1997-12-20 |
| EP0509008B1 (de) | 1994-11-30 |
| FI923099A0 (fi) | 1992-07-03 |
| US5391491A (en) | 1995-02-21 |
| AT393277B (de) | 1991-09-25 |
| CA2073168C (en) | 1999-04-27 |
| US5550051A (en) | 1996-08-27 |
| ATE114713T1 (de) | 1994-12-15 |
| DK0509008T3 (da) | 1995-04-18 |
| NO922622L (no) | 1992-07-23 |
| SK279200B6 (sk) | 1998-07-08 |
| FI923099A (fi) | 1992-07-03 |
| ES2067921T3 (es) | 1995-04-01 |
| DE59103696D1 (de) | 1995-01-12 |
| JPH05503843A (ja) | 1993-06-24 |
| NO310472B1 (no) | 2001-07-09 |
| ATA1890A (de) | 1991-02-15 |
| CA2073168A1 (en) | 1991-07-05 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| FI106241B (fi) | Biomassa virus/virusantigeenin tuottamiseksi | |
| Bratbak et al. | Viral control of Emiliania huxleyi blooms? | |
| EP1427817B1 (de) | Vermehrung von viren in zellkultur | |
| JP6067560B2 (ja) | 無血清培養及び浮遊培養に適応されたｍｄｃｋ細胞株及び前記細胞を使用してワクチン用ウイルスを製造する方法 | |
| CN101821381A (zh) | 处理用于生物反应器的细胞培养基的方法 | |
| KR20160096215A (ko) | 바이러스 항원 및 백신의 제조 방법 | |
| US6146891A (en) | Methods for cultivating cells and propagating viruses | |
| US7666654B2 (en) | Method for preparing viral material | |
| CN108546670A (zh) | 一种高粱原生质体的制备方法及制备的原生质体的转化方法 | |
| WO1998033886A1 (en) | Methods for cultivating cells and propagating viruses | |
| FI98377C (fi) | Adherentisti sitoutuneita soluja sisältävä matriisi ja menetelmä virus/virusantigeenin tuottamiseksi | |
| CN107858322B (zh) | 一种海马原代细胞培养体系的建立方法 | |
| Barreau et al. | An efficient and easy method of infection of mosquito larvae from virus-contaminated cell cultures | |
| KR101591729B1 (ko) | 박쥐나방 동충하초 균사체의 배양방법 및 그 배양방법으로 배양된 박쥐나방동충하초 균사체 | |
| CN103160477B (zh) | 一种提高乙型脑炎病毒效价的方法 | |
| Nikolay | Intensified yellow fever and Zika virus production in animal cell culture | |
| RU2082432C1 (ru) | Способ получения вакцины против клещевого энцефалита | |
| CN102327608A (zh) | 一种乙型脑炎疫苗的纯化方法 | |
| CN1513553B (zh) | Vero细胞森林脑炎灭活疫苗 | |
| RU2142816C1 (ru) | Способ получения антигерпетической вакцины и лекарственная форма на ее основе | |
| Rafajko | Routine establishment of serial lines of hamster embryo cells transformed by adenovirus type 12 | |
| WO2012129872A1 (zh) | 一种生物制品及其制备方法 | |
| Vazeille-Falcoz et al. | Unusual morphology of a virus which produces carbon dioxide sensitivity in mosquitoes | |
| RU2221041C1 (ru) | ШТАММ Bacillus licheniformis БСТ-1-ПРОДУЦЕНТ ПЕНИЦИЛЛИНАЗЫ И СПОСОБ ПОЛУЧЕНИЯ ПЕНИЦИЛЛИНАЗЫ | |
| RU2353649C2 (ru) | Способ культивирования лептоспир |