ES2855182T3 - Método de cromatografía - Google Patents

Método de cromatografía Download PDF

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Publication number
ES2855182T3
ES2855182T3 ES13713084T ES13713084T ES2855182T3 ES 2855182 T3 ES2855182 T3 ES 2855182T3 ES 13713084 T ES13713084 T ES 13713084T ES 13713084 T ES13713084 T ES 13713084T ES 2855182 T3 ES2855182 T3 ES 2855182T3
Authority
ES
Spain
Prior art keywords
separation unit
column
separation
sample
chromatography
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
ES13713084T
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English (en)
Spanish (es)
Inventor
Romas Skudas
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Merck Patent GmbH
Original Assignee
Merck Patent GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Merck Patent GmbH filed Critical Merck Patent GmbH
Application granted granted Critical
Publication of ES2855182T3 publication Critical patent/ES2855182T3/es
Active legal-status Critical Current
Anticipated expiration legal-status Critical

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 and B01D15/30 - B01D15/36, e.g. affinity, ligand exchange or chiral chromatography
    • B01D15/3804—Affinity chromatography
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
    • B01D15/18—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to flow patterns
    • B01D15/1814—Recycling of the fraction to be distributed
    • B01D15/1821—Simulated moving beds
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
    • B01D15/18—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to flow patterns
    • B01D15/1864—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to flow patterns using two or more columns
    • B01D15/1871—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to flow patterns using two or more columns placed in series
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/26—Conditioning of the fluid carrier; Flow patterns
    • G01N30/38—Flow patterns
    • G01N30/46—Flow patterns using more than one column
    • G01N30/461—Flow patterns using more than one column with serial coupling of separation columns
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/26—Conditioning of the fluid carrier; Flow patterns
    • G01N30/38—Flow patterns
    • G01N30/46—Flow patterns using more than one column
    • G01N30/468—Flow patterns using more than one column involving switching between different column configurations
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/26—Conditioning of the fluid carrier; Flow patterns
    • G01N30/38—Flow patterns
    • G01N30/44—Flow patterns using recycling of the fraction to be distributed

Landscapes

  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Health & Medical Sciences (AREA)
  • Sustainable Development (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
  • Peptides Or Proteins (AREA)
ES13713084T 2012-04-23 2013-03-26 Método de cromatografía Active ES2855182T3 (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP12002828.7A EP2656892A1 (en) 2012-04-23 2012-04-23 Chromatography method
US201261666453P 2012-06-29 2012-06-29
PCT/EP2013/000910 WO2013159858A1 (en) 2012-04-23 2013-03-26 Chromatography method

Publications (1)

Publication Number Publication Date
ES2855182T3 true ES2855182T3 (es) 2021-09-23

Family

ID=46052507

Family Applications (1)

Application Number Title Priority Date Filing Date
ES13713084T Active ES2855182T3 (es) 2012-04-23 2013-03-26 Método de cromatografía

Country Status (6)

Country Link
US (1) US9149738B2 (enExample)
EP (2) EP2656892A1 (enExample)
JP (1) JP2015520667A (enExample)
KR (1) KR102070566B1 (enExample)
ES (1) ES2855182T3 (enExample)
WO (1) WO2013159858A1 (enExample)

Families Citing this family (47)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2682168A1 (en) 2012-07-02 2014-01-08 Millipore Corporation Purification of biological molecules
RU2768003C2 (ru) 2013-03-08 2022-03-22 Джензим Корпорейшн Интегрированное непрерывное производство терапевтических белковых лекарственных веществ
TWI709569B (zh) 2014-01-17 2020-11-11 美商健臻公司 無菌層析樹脂及其用於製造方法的用途
TWI671312B (zh) * 2014-01-17 2019-09-11 美商健臻公司 無菌層析法及製法
GB201401010D0 (en) * 2014-01-21 2014-03-05 Nat Nuclear Lab Ltd Improved separation apparatus and method
DK3102935T3 (da) * 2014-02-07 2021-04-06 Cmc Biologics As Bioreaktoranordning og fremgangsmåde til fremstilling af en biopolymer
FR3018450B1 (fr) 2014-03-11 2016-04-15 Lab Francais Du Fractionnement Procede de preparation de proteines plasmatiques humaines
NZ724826A (en) 2014-04-16 2022-07-29 Cmc Biologics As A high cell density fill and draw fermentation process
CA2951408A1 (en) * 2014-06-25 2015-12-30 Jhl Biotech, Inc. Methods and reagents for purification of proteins
US11566082B2 (en) 2014-11-17 2023-01-31 Cytiva Bioprocess R&D Ab Mutated immunoglobulin-binding polypeptides
US10926191B2 (en) * 2014-12-12 2021-02-23 Cytiva Sweden Ab System for preparing solutions for chromatography
EP3037513A1 (de) * 2015-05-13 2016-06-29 Bayer Technology Services GmbH Verfahren zur kontinuierlichen elution eines produktes von chromatographiesäulen
US11835501B2 (en) 2015-07-13 2023-12-05 Sartorius Stedim Chromatography Systems Ltd. Optimizing operating binding capacity for a multiple column chromatography process
GB201515339D0 (en) * 2015-08-28 2015-10-14 Ge Healthcare Bio Sciences Ab A seperation matrix and a method of seperating antibodies
US10730908B2 (en) 2016-05-11 2020-08-04 Ge Healthcare Bioprocess R&D Ab Separation method
US10703774B2 (en) 2016-09-30 2020-07-07 Ge Healthcare Bioprocess R&D Ab Separation method
JP7031934B2 (ja) 2016-05-11 2022-03-08 サイティバ・バイオプロセス・アールアンドディ・アクチボラグ 分離マトリックス
US10889615B2 (en) 2016-05-11 2021-01-12 Cytiva Bioprocess R&D Ab Mutated immunoglobulin-binding polypeptides
WO2017194592A1 (en) 2016-05-11 2017-11-16 Ge Healthcare Bioprocess R&D Ab Method of storing a separation matrix
JP6987424B2 (ja) 2016-05-11 2022-01-05 サイティバ・バイオプロセス・アールアンドディ・アクチボラグ 分離マトリックスを洗浄および／または消毒する方法
EP3455243B1 (en) 2016-05-11 2021-03-24 Cytiva BioProcess R&D AB Separation matrix
US10654887B2 (en) 2016-05-11 2020-05-19 Ge Healthcare Bio-Process R&D Ab Separation matrix
EP3513170A4 (en) * 2016-09-17 2020-07-01 C Technologies MONITORING OF COMPOUNDS
US12448411B2 (en) 2016-09-30 2025-10-21 Cytiva Bioprocess R&D Ab Separation method
GB201622343D0 (en) * 2016-12-29 2017-02-15 Ge Healthcare Bio Sciences Ab Method in bioprocess purification system
EP3573734B1 (en) * 2017-01-30 2025-03-12 Regeneron Pharmaceuticals, Inc. Method for reducing bioburden in chromatography
GB201703116D0 (en) * 2017-02-27 2017-04-12 Ge Healthcare Bioprocess R&D Ab A seperation matrix and a method of seperating antibodies
EP3381529A1 (fr) 2017-03-29 2018-10-03 EMD Millipore Corporation Installation de traitement de liquide biologique
EP3381530A1 (fr) 2017-03-29 2018-10-03 EMD Millipore Corporation Installation de traitement de liquide biologique
GB201710130D0 (en) 2017-06-26 2017-08-09 Ge Healthcare Bioprocess R & D Ab A Method of seperation
CN107793469B (zh) * 2017-10-16 2021-07-30 上海药明生物技术有限公司 一种去除宿主细胞蛋白含量的亲和纯化工艺
GB201806752D0 (en) * 2018-04-25 2018-06-06 Ge Healthcare Bioprocess R&D Ab Method in bioprocess system
GB201810772D0 (en) 2018-06-29 2018-08-15 Ge Healthcare Bio Sciences Ab Method in bioprocess purification system
TW202448568A (zh) * 2018-07-02 2024-12-16 美商里珍納龍藥品有限公司 自混合物製備多肽之系統及方法
BR112021002724A2 (pt) * 2018-08-17 2021-07-20 Regeneron Pharmaceuticals, Inc. método para quantificar uma quantidade de um anticorpo heterodimérico, e, sistema de cromatografia
CN112638488A (zh) 2018-08-31 2021-04-09 建新公司 灭菌的层析树脂及其在制造工艺中的用途
US10981950B2 (en) * 2019-01-31 2021-04-20 Joanna Pezzini Therapeutic protein flow kit for a continuous purification system
TWI870412B (zh) * 2019-06-05 2025-01-21 美商建南德克公司 過載層析管柱之再生方法
FR3099066B1 (fr) 2019-07-26 2021-08-13 Novasep Process Procédé de purification d’une substance cible avec inactivation virale
US11906510B2 (en) * 2020-03-20 2024-02-20 Stanford Junior University Metabolic profiling by reverse-phase/ion-exchange mass spectrometry
KR20220158004A (ko) 2020-03-25 2022-11-29 메르크 파텐트 게엠베하 단백질 정제를 위한 인테인-결합 수지의 재사용
US20230406881A1 (en) * 2020-11-20 2023-12-21 Ares Trading S.A. Buffers and methods for purifying proteins
KR20240046607A (ko) 2021-09-28 2024-04-09 메르크 파텐트 게엠베하 연속 크로마토그래피 작업 동안의 크로마토그래피 수지의 모니터링 방법
KR20240054316A (ko) 2021-09-28 2024-04-25 메르크 파텐트 게엠베하 인라인 지속적 가압 탱크： “ｉｃｐｔ”를 사용한 공정 강화를 위한 통합 솔루션
CN115006884B (zh) * 2022-06-28 2023-08-15 江南大学 一种在线解耦式模拟移动床分离系统及其分离木糖和阿拉伯糖的方法
US20250041760A1 (en) * 2023-07-31 2025-02-06 Agilent Technologies, Inc. Column comprising particles
WO2025031868A1 (en) 2023-08-08 2025-02-13 Cytiva Sweden Ab Multi modular chromatography system

Family Cites Families (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP3478325B2 (ja) * 1997-12-25 2003-12-15 オルガノ株式会社 クロマト分離方法
JP4603114B2 (ja) * 1999-09-07 2010-12-22 オルガノ株式会社 液体に含まれる複数成分を分離する方法及び装置
US7691645B2 (en) * 2001-01-09 2010-04-06 Agilent Technologies, Inc. Immunosubtraction method
US20080312425A1 (en) * 2004-08-30 2008-12-18 Lonza Biologics Plc. Ion Exchange Chromatography and Purification of Antibodies
CN102417557B (zh) 2005-08-03 2015-03-11 默克专利股份公司 亲水交联聚合物
EP1775001A1 (en) * 2005-10-13 2007-04-18 Xendo Holding B.V. Device for chromatographic separations
WO2007144476A1 (fr) 2006-06-16 2007-12-21 Groupe Novasep Procede de separation sequence multicolonnes
US8569464B2 (en) 2006-12-21 2013-10-29 Emd Millipore Corporation Purification of proteins
US8362217B2 (en) 2006-12-21 2013-01-29 Emd Millipore Corporation Purification of proteins
WO2008079280A1 (en) 2006-12-21 2008-07-03 Millipore Corporation Purification of proteins
NZ577933A (en) 2007-01-22 2011-12-22 Genentech Inc Polyelectrolyte precipitation and purification of antibodies
WO2008153472A1 (en) * 2007-06-15 2008-12-18 Ge Healthcare Bio-Sciences Ab Chromatography method
US7618539B2 (en) 2008-03-31 2009-11-17 Ampac Fine Chemicals Llc Simulated moving bed chromatography for strongly retained compounds
WO2010035757A1 (ja) * 2008-09-25 2010-04-01 Jsr株式会社 アフィニティークロマトグラフィー用充填剤
CN102256993A (zh) 2008-12-16 2011-11-23 米利波尔公司 蛋白的纯化
JP5354094B2 (ja) * 2010-03-31 2013-11-27 Ｊｓｒ株式会社 アフィニティークロマトグラフィー用充填剤
CN101948399A (zh) * 2010-10-11 2011-01-19 江南大学 一种使用模拟移动床色谱连续分离纯化发酵液中缬氨酸的方法

Also Published As

Publication number Publication date
KR20150005996A (ko) 2015-01-15
WO2013159858A1 (en) 2013-10-31
KR102070566B1 (ko) 2020-01-29
US20130280788A1 (en) 2013-10-24
JP2015520667A (ja) 2015-07-23
US9149738B2 (en) 2015-10-06
EP2841176B1 (en) 2020-12-23
EP2656892A1 (en) 2013-10-30
EP2841176A1 (en) 2015-03-04

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