ES2855182T3 - Método de cromatografía - Google Patents

Método de cromatografía Download PDF

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Publication number
ES2855182T3
ES2855182T3 ES13713084T ES13713084T ES2855182T3 ES 2855182 T3 ES2855182 T3 ES 2855182T3 ES 13713084 T ES13713084 T ES 13713084T ES 13713084 T ES13713084 T ES 13713084T ES 2855182 T3 ES2855182 T3 ES 2855182T3
Authority
ES
Spain
Prior art keywords
separation unit
column
separation
sample
chromatography
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
ES13713084T
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English (en)
Spanish (es)
Inventor
Romas Skudas
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Merck Patent GmbH
Original Assignee
Merck Patent GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Merck Patent GmbH filed Critical Merck Patent GmbH
Application granted granted Critical
Publication of ES2855182T3 publication Critical patent/ES2855182T3/es
Active legal-status Critical Current
Anticipated expiration legal-status Critical

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 and B01D15/30 - B01D15/36, e.g. affinity, ligand exchange or chiral chromatography
    • B01D15/3804—Affinity chromatography
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
    • B01D15/18—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to flow patterns
    • B01D15/1814—Recycling of the fraction to be distributed
    • B01D15/1821—Simulated moving beds
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
    • B01D15/18—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to flow patterns
    • B01D15/1864—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to flow patterns using two or more columns
    • B01D15/1871—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to flow patterns using two or more columns placed in series
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/26—Conditioning of the fluid carrier; Flow patterns
    • G01N30/38—Flow patterns
    • G01N30/46—Flow patterns using more than one column
    • G01N30/461—Flow patterns using more than one column with serial coupling of separation columns
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/26—Conditioning of the fluid carrier; Flow patterns
    • G01N30/38—Flow patterns
    • G01N30/46—Flow patterns using more than one column
    • G01N30/468—Flow patterns using more than one column involving switching between different column configurations
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/26—Conditioning of the fluid carrier; Flow patterns
    • G01N30/38—Flow patterns
    • G01N30/44—Flow patterns using recycling of the fraction to be distributed

Landscapes

  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Sustainable Development (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
  • Peptides Or Proteins (AREA)
ES13713084T 2012-04-23 2013-03-26 Método de cromatografía Active ES2855182T3 (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP12002828.7A EP2656892A1 (en) 2012-04-23 2012-04-23 Chromatography method
US201261666453P 2012-06-29 2012-06-29
PCT/EP2013/000910 WO2013159858A1 (en) 2012-04-23 2013-03-26 Chromatography method

Publications (1)

Publication Number Publication Date
ES2855182T3 true ES2855182T3 (es) 2021-09-23

Family

ID=46052507

Family Applications (1)

Application Number Title Priority Date Filing Date
ES13713084T Active ES2855182T3 (es) 2012-04-23 2013-03-26 Método de cromatografía

Country Status (6)

Country Link
US (1) US9149738B2 (enExample)
EP (2) EP2656892A1 (enExample)
JP (1) JP2015520667A (enExample)
KR (1) KR102070566B1 (enExample)
ES (1) ES2855182T3 (enExample)
WO (1) WO2013159858A1 (enExample)

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TWI709570B (zh) 2014-01-17 2020-11-11 美商健臻公司 無菌層析法及製法
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GB201401010D0 (en) * 2014-01-21 2014-03-05 Nat Nuclear Lab Ltd Improved separation apparatus and method
WO2015117883A1 (en) * 2014-02-07 2015-08-13 Cmc Biologics A/S A bioreactor arrangement and continuous process for producing and capturing a biopolymer
FR3018450B1 (fr) 2014-03-11 2016-04-15 Lab Francais Du Fractionnement Procede de preparation de proteines plasmatiques humaines
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SG11201610431WA (en) 2014-06-25 2017-01-27 Jhl Biotech Inc Methods and reagents for purification of proteins
US11566082B2 (en) 2014-11-17 2023-01-31 Cytiva Bioprocess R&D Ab Mutated immunoglobulin-binding polypeptides
US10926191B2 (en) 2014-12-12 2021-02-23 Cytiva Sweden Ab System for preparing solutions for chromatography
EP3037513A1 (de) * 2015-05-13 2016-06-29 Bayer Technology Services GmbH Verfahren zur kontinuierlichen elution eines produktes von chromatographiesäulen
US11835501B2 (en) 2015-07-13 2023-12-05 Sartorius Stedim Chromatography Systems Ltd. Optimizing operating binding capacity for a multiple column chromatography process
GB201515339D0 (en) 2015-08-28 2015-10-14 Ge Healthcare Bio Sciences Ab A seperation matrix and a method of seperating antibodies
US10703774B2 (en) 2016-09-30 2020-07-07 Ge Healthcare Bioprocess R&D Ab Separation method
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US10889615B2 (en) 2016-05-11 2021-01-12 Cytiva Bioprocess R&D Ab Mutated immunoglobulin-binding polypeptides
JP7031934B2 (ja) 2016-05-11 2022-03-08 サイティバ・バイオプロセス・アールアンドディ・アクチボラグ 分離マトリックス
EP3455243B1 (en) 2016-05-11 2021-03-24 Cytiva BioProcess R&D AB Separation matrix
US10654887B2 (en) 2016-05-11 2020-05-19 Ge Healthcare Bio-Process R&D Ab Separation matrix
CN109311949B (zh) 2016-05-11 2022-09-16 思拓凡生物工艺研发有限公司 储存分离基质的方法
US10730908B2 (en) 2016-05-11 2020-08-04 Ge Healthcare Bioprocess R&D Ab Separation method
EP3513170A4 (en) * 2016-09-17 2020-07-01 C Technologies MONITORING OF COMPOUNDS
US12448411B2 (en) 2016-09-30 2025-10-21 Cytiva Bioprocess R&D Ab Separation method
GB201622343D0 (en) * 2016-12-29 2017-02-15 Ge Healthcare Bio Sciences Ab Method in bioprocess purification system
SG11201905510XA (en) * 2017-01-30 2019-08-27 Regeneron Pharma Compositions and methods for reducing bioburden in chromatography
GB201703116D0 (en) 2017-02-27 2017-04-12 Ge Healthcare Bioprocess R&D Ab A seperation matrix and a method of seperating antibodies
EP3381529A1 (fr) * 2017-03-29 2018-10-03 EMD Millipore Corporation Installation de traitement de liquide biologique
EP3381530A1 (fr) * 2017-03-29 2018-10-03 EMD Millipore Corporation Installation de traitement de liquide biologique
GB201710130D0 (en) 2017-06-26 2017-08-09 Ge Healthcare Bioprocess R & D Ab A Method of seperation
CN107793469B (zh) * 2017-10-16 2021-07-30 上海药明生物技术有限公司 一种去除宿主细胞蛋白含量的亲和纯化工艺
GB201806752D0 (en) * 2018-04-25 2018-06-06 Ge Healthcare Bioprocess R&D Ab Method in bioprocess system
GB201810772D0 (en) 2018-06-29 2018-08-15 Ge Healthcare Bio Sciences Ab Method in bioprocess purification system
TWI853823B (zh) * 2018-07-02 2024-09-01 美商里珍納龍藥品有限公司 自混合物製備多肽之系統及方法
JP7527273B2 (ja) * 2018-08-17 2024-08-02 リジェネロン・ファーマシューティカルズ・インコーポレイテッド 多量体タンパク質の量および純度を決定するための方法およびクロマトグラフィーシステム
HK40046210A (en) 2018-08-31 2021-10-29 Genzyme Corporation Sterile chromatography resin and use thereof in manufacturing processes
US10981950B2 (en) * 2019-01-31 2021-04-20 Joanna Pezzini Therapeutic protein flow kit for a continuous purification system
TWI870412B (zh) * 2019-06-05 2025-01-21 美商建南德克公司 過載層析管柱之再生方法
FR3099066B1 (fr) 2019-07-26 2021-08-13 Novasep Process Procédé de purification d’une substance cible avec inactivation virale
US11906510B2 (en) * 2020-03-20 2024-02-20 Stanford Junior University Metabolic profiling by reverse-phase/ion-exchange mass spectrometry
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EP4247832A1 (en) * 2020-11-20 2023-09-27 Ares Trading S.A. Buffers and methods for purifying proteins
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JP2024535429A (ja) 2021-09-28 2024-09-30 メルク パテント ゲーエムベーハー 連続クロマトグラフィ動作中のクロマトグラフィ樹脂をモニタリングするための方法
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Also Published As

Publication number Publication date
US20130280788A1 (en) 2013-10-24
KR102070566B1 (ko) 2020-01-29
WO2013159858A1 (en) 2013-10-31
EP2841176A1 (en) 2015-03-04
KR20150005996A (ko) 2015-01-15
EP2656892A1 (en) 2013-10-30
JP2015520667A (ja) 2015-07-23
EP2841176B1 (en) 2020-12-23
US9149738B2 (en) 2015-10-06

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