ES2793249T3 - Método para aislar moléculas de ARN pequeño - Google Patents

Método para aislar moléculas de ARN pequeño Download PDF

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Publication number
ES2793249T3
ES2793249T3 ES18166823T ES18166823T ES2793249T3 ES 2793249 T3 ES2793249 T3 ES 2793249T3 ES 18166823 T ES18166823 T ES 18166823T ES 18166823 T ES18166823 T ES 18166823T ES 2793249 T3 ES2793249 T3 ES 2793249T3
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Prior art keywords
lysate
rna
rna molecules
alcohol
solution
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ES18166823T
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English (en)
Spanish (es)
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Richard Conrad
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Life Technologies Corp
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Life Technologies Corp
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H1/00—Processes for the preparation of sugar derivatives
    • C07H1/06—Separation; Purification
    • C07H1/08—Separation; Purification from natural products
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor

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  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
ES18166823T 2003-07-25 2004-07-26 Método para aislar moléculas de ARN pequeño Expired - Lifetime ES2793249T3 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US49032503P 2003-07-25 2003-07-25
US10/667,126 US20050059024A1 (en) 2003-07-25 2003-09-19 Methods and compositions for isolating small RNA molecules

Publications (1)

Publication Number Publication Date
ES2793249T3 true ES2793249T3 (es) 2020-11-13

Family

ID=34118826

Family Applications (2)

Application Number Title Priority Date Filing Date
ES18166823T Expired - Lifetime ES2793249T3 (es) 2003-07-25 2004-07-26 Método para aislar moléculas de ARN pequeño
ES14183292T Expired - Lifetime ES2721631T3 (es) 2003-07-25 2004-07-26 Método para aislar pequeñas moléculas de ARN

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ES14183292T Expired - Lifetime ES2721631T3 (es) 2003-07-25 2004-07-26 Método para aislar pequeñas moléculas de ARN

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US (5) US20050059024A1 (enExample)
EP (4) EP2298891B2 (enExample)
JP (1) JP2007500008A (enExample)
ES (2) ES2793249T3 (enExample)
WO (1) WO2005012523A1 (enExample)

Families Citing this family (114)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7078224B1 (en) * 1999-05-14 2006-07-18 Promega Corporation Cell concentration and lysate clearance using paramagnetic particles
US7745180B2 (en) * 2002-04-24 2010-06-29 Hitachi Chemical Co., Ltd. Device and method for high-throughput quantification of mRNA from whole blood
US20050059024A1 (en) 2003-07-25 2005-03-17 Ambion, Inc. Methods and compositions for isolating small RNA molecules
WO2005012487A2 (en) * 2003-08-01 2005-02-10 Invitrogen Corporation Compositions and methods for preparing short rna molecules and other nucleic acids
IL179285A (en) 2004-05-14 2011-04-28 Rosetta Genomics Ltd Micrornas and uses thereof
US7795419B2 (en) 2004-05-26 2010-09-14 Rosetta Genomics Ltd. Viral and viral associated miRNAs and uses thereof
AU2005250432B2 (en) 2004-05-28 2011-09-15 Asuragen, Inc. Methods and compositions involving microRNA
WO2008073922A2 (en) * 2006-12-08 2008-06-19 Asuragen, Inc. Functions and targets of let-7 micro rnas
CA2857881A1 (en) 2004-11-12 2006-12-28 Asuragen, Inc. Methods and compositions involving mirna and mirna inhibitor molecules
WO2006088601A2 (en) * 2005-01-21 2006-08-24 New York Blood Center, Inc., The Method for extraction and identification of nucleic acids
JP2006292367A (ja) * 2005-04-05 2006-10-26 Mitsubishi Rayon Co Ltd ｍｉＲＮＡ検出用マイクロアレイ
WO2006130632A2 (en) 2005-05-31 2006-12-07 Invitrogen Corporation Separation and purification of nucleic acid from paraffin-containing samples
JP2009501531A (ja) * 2005-07-15 2009-01-22 アプレラ コーポレイション Ｒｎａプロファイリングのための方法
EP1924708A4 (en) * 2005-09-12 2010-03-10 Applera Corp PREPARATION METHOD FOR PURE MIRNA SAMPLE
EP1941058A4 (en) * 2005-10-27 2010-01-20 Rosetta Inpharmatics Llc NUCLEIC ACID REINFORCEMENT FROM NON-INCIDENTIAL PRIMERS
EP1963526A4 (en) 2005-12-09 2009-11-18 Promega Corp PURIFICATION OF NUCLEIC ACID WITH A BINDING MATRIX
KR100658605B1 (ko) 2006-01-31 2006-12-19 한국생명공학연구원 시아노박테리아로부터 고효율로 ｒｎａ를 추출하는 방법
US20070202511A1 (en) 2006-02-28 2007-08-30 Sigma-Aldrich Co. Methods and compositions for the rapid isolation of small RNA molecules
US20070207483A1 (en) * 2006-03-02 2007-09-06 Bio-Rad Laboratories, Inc. BUFFERS FOR DETECTION OF mRNA SEPARATED IN A MICROFLUIDIC DEVICE
WO2007103485A2 (en) * 2006-03-08 2007-09-13 Promega Corporation Small rna purification
US8679741B2 (en) * 2006-05-31 2014-03-25 Sequenom, Inc. Methods and compositions for the extraction and amplification of nucleic acid from a sample
WO2008036776A2 (en) * 2006-09-19 2008-03-27 Asuragen, Inc. Mir-15, mir-26, mir -31,mir -145, mir-147, mir-188, mir-215, mir-216 mir-331, mmu-mir-292-3p regulated genes and pathways as targets for therapeutic intervention
US20080081330A1 (en) * 2006-09-28 2008-04-03 Helicos Biosciences Corporation Method and devices for analyzing small RNA molecules
US20080131878A1 (en) * 2006-12-05 2008-06-05 Asuragen, Inc. Compositions and Methods for the Detection of Small RNA
CN101622349A (zh) * 2006-12-08 2010-01-06 奥斯瑞根公司 作为治疗性干预靶标的miR-21调节的基因和途径
WO2008073920A2 (en) * 2006-12-08 2008-06-19 Asuragen, Inc. Mir-21 regulated genes and pathways as targets for therapeutic intervention
AU2007333106A1 (en) * 2006-12-08 2008-06-19 Asuragen, Inc. miR-20 regulated genes and pathways as targets for therapeutic intervention
EP1932913B1 (en) 2006-12-11 2013-01-16 Roche Diagnostics GmbH Nucleic acid isolation using polidocanol and derivatives
CN102776173B (zh) * 2006-12-11 2014-10-29 霍夫曼-拉罗奇有限公司 使用聚多卡醇和衍生物的核酸分离
WO2008071384A1 (en) * 2006-12-13 2008-06-19 Roche Diagnostics Gmbh Use of tde for the isolation of nucleic acids
US20090175827A1 (en) * 2006-12-29 2009-07-09 Byrom Mike W miR-16 REGULATED GENES AND PATHWAYS AS TARGETS FOR THERAPEUTIC INTERVENTION
US20090232893A1 (en) * 2007-05-22 2009-09-17 Bader Andreas G miR-143 REGULATED GENES AND PATHWAYS AS TARGETS FOR THERAPEUTIC INTERVENTION
JP2010529966A (ja) * 2007-06-08 2010-09-02 アシュラジェン インコーポレイテッド 治療的介入の標的としてｍｉＲ−３４によって調節される遺伝子および経路
MY157651A (en) * 2007-07-23 2016-07-15 Yiap Beow Chin A method, kit and apparatus for extracting biological materials
US20090053704A1 (en) * 2007-08-24 2009-02-26 Natalia Novoradovskaya Stabilization of nucleic acids on solid supports
WO2009036332A1 (en) 2007-09-14 2009-03-19 Asuragen, Inc. Micrornas differentially expressed in cervical cancer and uses thereof
US20090186015A1 (en) * 2007-10-18 2009-07-23 Latham Gary J Micrornas differentially expressed in lung diseases and uses thereof
KR101432034B1 (ko) * 2007-11-16 2014-08-21 삼성전자주식회사 코스모트로픽 염을 이용한 고체 지지체에서의 작은 ｒｎａ분리 방법
AU2008329833B2 (en) * 2007-11-30 2014-04-17 Global Life Sciences Solutions Usa Llc Method for isolation of genomic DNA, RNA and proteins from a single sample
WO2009070805A2 (en) * 2007-12-01 2009-06-04 Asuragen, Inc. Mir-124 regulated genes and pathways as targets for therapeutic intervention
WO2009086156A2 (en) * 2007-12-21 2009-07-09 Asuragen, Inc. Mir-10 regulated genes and pathways as targets for therapeutic intervention
US20090263803A1 (en) * 2008-02-08 2009-10-22 Sylvie Beaudenon Mirnas differentially expressed in lymph nodes from cancer patients
WO2009154835A2 (en) * 2008-03-26 2009-12-23 Asuragen, Inc. Compositions and methods related to mir-16 and therapy of prostate cancer
US20090258928A1 (en) * 2008-04-08 2009-10-15 Asuragen, Inc. Methods and compositions for diagnosing and modulating human papillomavirus (hpv)
EP2990487A1 (en) * 2008-05-08 2016-03-02 Asuragen, INC. Compositions and methods related to mirna modulation of neovascularization or angiogenesis
AU2009246363B2 (en) 2008-05-13 2013-10-24 Gen-Probe Incorporated Inactivatable target capture oligomers for use in the selective hybridization and capture of target nucleic acid sequences
EP2128169A1 (de) * 2008-05-30 2009-12-02 Qiagen GmbH Verfahren zur Isolierung von kurzkettigen Nukleinsäuren
EP2315850B1 (en) 2008-07-18 2015-02-25 TrovaGene, Inc. Methods for pcr-based detection of "ultra short" nucleic acid sequences
DE102008045705A1 (de) 2008-09-04 2010-04-22 Macherey, Nagel Gmbh & Co. Kg Handelsgesellschaft Verfahren zur Gewinnung von kurzer RNA sowie Kit hierfür
EP2324131A4 (en) * 2008-09-17 2013-02-27 Ge Healthcare Bio Sciences PROCESS FOR ISOLATING SMALL RNA
WO2010056737A2 (en) * 2008-11-11 2010-05-20 Mirna Therapeutics, Inc. Methods and compositions involving mirnas in cancer stem cells
BRPI0921043A2 (pt) 2008-11-12 2018-08-07 Caris Life Sciences Luxembourg Holdings métodos e sistemas para usar exossomas para determinar fenótipos
EP2193831A1 (de) 2008-12-05 2010-06-09 Qiagen GmbH Parallel-Extraktion von unterschiedlichen Biomolekülen aus Formalin-fixiertem Gewebe
EP2414545B1 (en) 2009-04-03 2017-01-11 Sequenom, Inc. Nucleic acid preparation compositions and methods
NZ619527A (en) 2009-04-14 2015-06-26 Nestec Sa Inflammatory bowel disease prognostics
EP2264168B1 (de) * 2009-06-18 2014-12-17 Qiagen GmbH Verfahren zur Isolierung von Nukleinsäuren
WO2011008553A1 (en) * 2009-06-29 2011-01-20 Life Technologies Corporation Rna sample stabilization in the presence of a transitional metal
US8222397B2 (en) * 2009-08-28 2012-07-17 Promega Corporation Methods of optimal purification of nucleic acids and kit for use in performing such methods
US8039613B2 (en) 2009-08-28 2011-10-18 Promega Corporation Methods of purifying a nucleic acid and formulation and kit for use in performing such methods
WO2011060098A1 (en) 2009-11-10 2011-05-19 Prometheus Laboratories Inc. Methods for predicting post-surgery risk associated with ileal pouch-anal anastomosis
CA2782284A1 (en) 2009-11-30 2011-06-03 Caris Life Sciences Luxembourg Holdings, S.A.R.L. Methods and systems for isolating, storing, and analyzing vesicles
US20140148348A1 (en) 2010-01-13 2014-05-29 Christine Kuslich Dectection of gastrointestinal disorders
US8846631B2 (en) 2010-01-14 2014-09-30 Regulus Therapeutics Inc. MicroRNA compositions and methods
CN102884191B (zh) 2010-02-26 2018-04-27 凯杰有限公司 用于平行分离和/或纯化rna和dna的方法
AU2011223789A1 (en) 2010-03-01 2012-09-20 Caris Life Sciences Switzerland Holdings Gmbh Biomarkers for theranostics
KR20130043104A (ko) 2010-04-06 2013-04-29 카리스 라이프 사이언스 룩셈부르크 홀딩스 질병용 순환 생물학적 지표들
US20120077198A1 (en) * 2010-07-30 2012-03-29 Ambergen, Inc Compositions And Methods For Cancer Testing
JP5867402B2 (ja) * 2010-09-24 2016-02-24 和光純薬工業株式会社 ｓｍａｌｌＲＮＡの取得方法
ES2629890T3 (es) 2010-11-17 2017-08-16 Interpace Diagnostics, Llc miARN como biomarcadores para distinguir entre neoplasias de tiroides benignas y malignas
US20120149088A1 (en) 2010-11-30 2012-06-14 Life Technologies Corporation Alkylene glycols and polymers and copolymers thereof for direct isolation of nucleic acid from embedded samples
US9051563B2 (en) 2011-01-14 2015-06-09 Zymo Research Corporation Nucleic acid purification
EP3733870A3 (en) * 2011-01-14 2021-01-27 Life Technologies Corporation Methods for identification and quantification of mirnas
JP2014506791A (ja) 2011-02-03 2014-03-20 マーナ セラピューティクス インコーポレイテッド ｍｉＲ−３４の合成模倣体
WO2012174293A2 (en) 2011-06-14 2012-12-20 Nestec Sa Methods for identifying inflammatory bowel disease patients with dysplasia or cancer
WO2013040251A2 (en) 2011-09-13 2013-03-21 Asurgen, Inc. Methods and compositions involving mir-135b for distinguishing pancreatic cancer from benign pancreatic disease
GB2501238B (en) 2012-03-16 2018-10-24 Cambridge Entpr Ltd Methods for obtaining liquid from a solid phase
US9206469B2 (en) 2012-07-18 2015-12-08 Zymo Research Corporation Nucleic acid purification
US9695416B2 (en) 2012-07-18 2017-07-04 Siemens Healthcare Diagnostics Inc. Method of normalizing biological samples
CA2880136C (en) 2012-09-03 2023-08-15 Qiagen Gmbh Method for isolating rna including small rna with high yield
US20140272967A1 (en) * 2013-03-13 2014-09-18 Abbott Molecular Inc. Systems and methods for isolating nucleic acids
US20140287408A1 (en) * 2013-03-13 2014-09-25 Abbott Molecular Inc. Target sequence enrichment
EP2970955B1 (en) 2013-03-14 2018-11-14 Translate Bio, Inc. Methods for purification of messenger rna
JP6431522B2 (ja) * 2013-03-15 2018-11-28 アボツト・モレキユラー・インコーポレイテツド 核酸の精製のための１工程法
US9828600B2 (en) 2013-09-20 2017-11-28 University Of Massachusetts Compositions and methods for constructing cDNA libraries that allow for mapping the 5′ and 3′ ends of RNAs
SG11201608725YA (en) * 2014-04-25 2016-11-29 Shire Human Genetic Therapies Methods for purification of messenger rna
WO2016009059A1 (en) 2014-07-17 2016-01-21 Qiagen Gmbh Method for isolating rna with high yield
IL234246A0 (en) * 2014-08-21 2014-11-30 Omrix Biopharmaceuticals Ltd Stabilized thrombin
US10000750B2 (en) 2014-10-09 2018-06-19 Promega Corporation Method of isolating nucleic acid
EP3034619B1 (de) * 2014-12-16 2018-10-17 microBIOMix GmbH Modifizierte Oligonukleotide und Verfahren zum Einschleusen von Primern oder Kontrollmolekülen oder Sonden in geschlossene PCR-Systeme
US10968443B2 (en) * 2014-12-31 2021-04-06 The Rockefeller University Method of RNA isolation from clinical samples
EP3368669B1 (en) 2015-10-28 2021-03-10 Mirnagreen S.r.l. Methods for extracting bioactive small rnas from plants and mushrooms
US10214739B2 (en) * 2015-10-30 2019-02-26 Axagarius Gmbh & Co. Kg RNA binding solution
WO2017136482A1 (en) 2016-02-01 2017-08-10 The Board Of Regents Of The University Of Nebraska Method of identifying important methylome features and use thereof
US10287625B2 (en) * 2016-03-18 2019-05-14 Norgen Biotek Corp. Methods and kits for separating nucleic acids by size
CN107267497B (zh) * 2016-04-08 2018-06-22 北京爱普拜生物技术有限公司 一种通用型总rna提取试剂盒及方法
SMT202100681T1 (it) * 2016-04-22 2022-01-10 BioNTech SE Procedimenti per fornire rna a singolo filamento
HRP20230345T1 (hr) 2017-01-16 2023-06-09 Spectrum Solutions L.L.C. Otopina za očuvanje nukleinske kiseline i načini uporabe
KR101885038B1 (ko) * 2017-09-27 2018-08-03 전남대학교산학협력단 바이러스 rna를 추출하는 방법
US11434480B2 (en) 2017-09-27 2022-09-06 Industry Foundation Of Chonnam National University Viral RNA extracting composition and viral RNA extracting method
US11725200B2 (en) 2017-09-27 2023-08-15 Qiagen Gmbh Method for isolating RNA with high yield
WO2019116234A1 (en) 2017-12-12 2019-06-20 Nestec S.A. Methods for determining microbiome ribosomal rna composition changes
CA3086996A1 (en) * 2017-12-27 2019-07-04 Toray Industries, Inc. Method for collecting nucleic acid
KR102094079B1 (ko) * 2018-07-27 2020-03-27 전남대학교산학협력단 실리카 코팅 자성 비드를 이용한 바이러스 rna 추출용 조성물 및 이를 이용한 바이러스 rna 추출 방법
CN112930396B (zh) 2018-08-24 2024-05-24 川斯勒佰尔公司 用于纯化信使rna的方法
CN109569023B (zh) * 2018-11-29 2021-03-02 杭州立昂科技有限公司 参杂去污剂的干燥水凝胶颗粒及大分子浓缩和比活性提升
SI3896159T1 (sl) * 2020-04-17 2025-08-29 Sartorius Bia Separations D.O.O. Metoda čiščenja enoverižne rnk z uporabo anionskega izmenjevalca
CN111254141B (zh) 2020-04-28 2020-08-04 博奥生物集团有限公司 核酸提取组合物及其应用，含有该核酸提取组合物的试剂、试剂盒
CA3184255A1 (en) 2020-07-07 2022-01-13 Markus Mueller Method for isolating nucleic acid
KR102415720B1 (ko) * 2020-10-08 2022-07-01 주식회사 인바이러스테크 폴리페놀 및/또는 다당류가 풍부하게 함유된 시료로부터 핵산을 추출하는 방법
KR102421648B1 (ko) * 2020-10-08 2022-07-18 주식회사 인바이러스테크 Rna 분해효소가 풍부하게 함유된 시료로부터 핵산을 추출하는 방법
CN112746055A (zh) * 2020-12-30 2021-05-04 华南农业大学 一种牛奶体细胞分离过程优化工艺
EP4134432A1 (en) * 2021-08-11 2023-02-15 Mirnagreen S.r.l. Improved method for small rna purification
CN114517196A (zh) * 2022-02-25 2022-05-20 杭州诺辉健康科技有限公司 一种血浆游离miRNA提取方法及其应用
CN114426970A (zh) * 2022-03-17 2022-05-03 黑龙江省科学院高技术研究院 一种适合杨树rna的提取方法

Family Cites Families (94)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4283489A (en) * 1977-09-23 1981-08-11 The Regents Of The University Of California Purification of nucleotide sequences suitable for expression in bacteria
US4883750A (en) 1984-12-13 1989-11-28 Applied Biosystems, Inc. Detection of specific sequences in nucleic acids
GB8507706D0 (en) 1985-03-25 1985-05-01 Genetics Int Inc Magnetic nucleic acid sequences
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4800159A (en) 1986-02-07 1989-01-24 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences
DE8615833U1 (de) 1986-04-17 1987-02-12 Preuss, Herbert, 6070 Langen Florfaserbürste zur Reinigung von Textilgeweben
AU622104B2 (en) 1987-03-11 1992-04-02 Sangtec Molecular Diagnostics Ab Method of assaying of nucleic acids, a reagent combination and kit therefore
IL86724A (en) 1987-06-19 1995-01-24 Siska Diagnostics Inc Methods and kits for amplification and testing of nucleic acid sequences
US5115018A (en) * 1987-08-24 1992-05-19 Allied-Signal Inc. High-impact, styrenic polymer/thermoplastic polymer grafted blends
US4843155A (en) * 1987-11-19 1989-06-27 Piotr Chomczynski Product and process for isolating RNA
CA1323293C (en) 1987-12-11 1993-10-19 Keith C. Backman Assay using template-dependent nucleic acid probe reorganization
JP2846018B2 (ja) 1988-01-21 1999-01-13 ジェネンテク，インコーポレイテッド 核酸配列の増幅および検出
CA1340807C (en) 1988-02-24 1999-11-02 Lawrence T. Malek Nucleic acid amplification process
US5858652A (en) 1988-08-30 1999-01-12 Abbott Laboratories Detection and amplification of target nucleic acid sequences
WO1990006045A2 (en) 1988-11-21 1990-06-14 Dynal As Nucleic acid probes
NL8900725A (nl) 1989-03-23 1990-10-16 Az Univ Amsterdam Werkwijze en combinatie van middelen voor het isoleren van nucleinezuur.
US5234809A (en) * 1989-03-23 1993-08-10 Akzo N.V. Process for isolating nucleic acid
US5143854A (en) 1989-06-07 1992-09-01 Affymax Technologies N.V. Large scale photolithographic solid phase synthesis of polypeptides and receptor binding screening thereof
US5744101A (en) 1989-06-07 1998-04-28 Affymax Technologies N.V. Photolabile nucleoside protecting groups
US5128247A (en) 1989-08-14 1992-07-07 Board Of Regents, The University Of Texas System Methods for isolation of nucleic acids from eukaryotic and prokaryotic sources
US5545522A (en) 1989-09-22 1996-08-13 Van Gelder; Russell N. Process for amplifying a target polynucleotide sequence using a single primer-promoter complex
MD1652G2 (ro) * 1991-03-29 2001-12-31 Sanofi-Synthelabo Proteină cu activitate de tip citochină, ADN recombinant, codificator pentru această proteină, celule şi microorganisme transformate, vector de expresie, procedeu de obţinere a proteinei şi remediu care conţine această proteină
CA2067711C (en) 1991-05-03 2000-08-08 Daniel Lee Woodard Solid phase extraction purification of dna
US5260048A (en) 1991-05-08 1993-11-09 Streck Laboratories, Inc. Tissue fixative solution and method
US5196182A (en) 1991-05-08 1993-03-23 Streck Laboratories, Inc. Tissue fixative
US5155018A (en) 1991-07-10 1992-10-13 Hahnemann University Process and kit for isolating and purifying RNA from biological sources
US5412087A (en) 1992-04-24 1995-05-02 Affymax Technologies N.V. Spatially-addressable immobilization of oligonucleotides and other biological polymers on surfaces
DE69333650T2 (de) 1992-02-19 2006-01-12 The Public Health Research Institute Of The City Of New York, Inc. Neue anordnungn von oligonukleotiden und ihr nutzen zum sortieren, isolieren, sequenzieren und manipulieren von nukleinsäuren
US5514545A (en) 1992-06-11 1996-05-07 Trustees Of The University Of Pennsylvania Method for characterizing single cells based on RNA amplification for diagnostics and therapeutics
US5480972A (en) 1992-10-30 1996-01-02 The University Of Melbourne Allergenic proteins from Johnson grass pollen
US5846709A (en) 1993-06-15 1998-12-08 Imclone Systems Incorporated Chemical process for amplifying and detecting nucleic acid sequences
DE4321904B4 (de) * 1993-07-01 2013-05-16 Qiagen Gmbh Verfahren zur chromatographischen Reinigung und Trennung von Nucleinsäuregemischen
FR2708288B1 (fr) 1993-07-26 1995-09-01 Bio Merieux Procédé d'amplification d'acides nucléiques par transcription utilisant le déplacement, réactifs et nécessaire pour la mise en Óoeuvre de ce procédé.
CA2170604C (en) 1993-08-30 2007-03-13 Vikas V. Padhye Nucleic acid purification compositions and methods
US6309823B1 (en) 1993-10-26 2001-10-30 Affymetrix, Inc. Arrays of nucleic acid probes for analyzing biotransformation genes and methods of using the same
DK0743950T3 (da) * 1994-02-11 2002-03-25 Qiagen Gmbh Fremgangsmåde til adskillelse af dobbeltstrengede/enkeltstrengede nukleinsyre-strukturer
US5928905A (en) 1995-04-18 1999-07-27 Glaxo Group Limited End-complementary polymerase reaction
NZ287118A (en) 1994-05-28 1999-01-28 Tepnel Medical Ltd Method of detecting and amplifying oligonucleotides
US5942391A (en) 1994-06-22 1999-08-24 Mount Sinai School Of Medicine Nucleic acid amplification method: ramification-extension amplification method (RAM)
US5876924A (en) 1994-06-22 1999-03-02 Mount Sinai School Of Medicine Nucleic acid amplification method hybridization signal amplification method (HSAM)
CA2195562A1 (en) 1994-08-19 1996-02-29 Pe Corporation (Ny) Coupled amplification and ligation method
US5935825A (en) 1994-11-18 1999-08-10 Shimadzu Corporation Process and reagent for amplifying nucleic acid sequences
US5843650A (en) 1995-05-01 1998-12-01 Segev; David Nucleic acid detection and amplification by chemical linkage of oligonucleotides
JP2965131B2 (ja) 1995-07-07 1999-10-18 東洋紡績株式会社 核酸結合用磁性担体およびそれを用いる核酸単離方法
US5916779A (en) 1995-09-21 1999-06-29 Becton, Dickinson And Company Strand displacement amplification of RNA targets
US5612473A (en) 1996-01-16 1997-03-18 Gull Laboratories Methods, kits and solutions for preparing sample material for nucleic acid amplification
US6168918B1 (en) 1996-01-31 2001-01-02 American Home Products Corp. Method of detecting foreign DNA integrated in eukaryotic chromosomes
DE29601618U1 (de) * 1996-01-31 1996-07-04 InViTek GmbH, 13125 Berlin Vorrichtung zur gleichzeitigen multiplen Isolierung
CA2188816C (en) * 1996-04-23 2009-09-22 Takeda Chemical Industries, Ltd. Composition containing .alpha.-fodrin or .alpha.- fodrin fragment protein
US5928906A (en) 1996-05-09 1999-07-27 Sequenom, Inc. Process for direct sequencing during template amplification
US5939291A (en) 1996-06-14 1999-08-17 Sarnoff Corporation Microfluidic method for nucleic acid amplification
US5990302A (en) * 1996-07-12 1999-11-23 Toyo Boseki Kabushiki Kaisha Method for isolating ribonucleic acid
US5849546A (en) 1996-09-13 1998-12-15 Epicentre Technologies Corporation Methods for using mutant RNA polymerases with reduced discrimination between non-canonical and canonical nucleoside triphosphates
JP2001500741A (ja) 1996-09-19 2001-01-23 アフィメトリックス，インコーポレイテッド 分子配列サインの同定およびそれに関する方法
US5849497A (en) 1997-04-03 1998-12-15 The Research Foundation Of State University Of New York Specific inhibition of the polymerase chain reaction using a non-extendable oligonucleotide blocker
EP0975807B1 (en) * 1997-05-02 2006-09-27 Gen-Probe Incorporated Two-step hybridization and capture of a polynucleotide
US6218531B1 (en) * 1997-06-25 2001-04-17 Promega Corporation Method of isolating RNA
US5866366A (en) 1997-07-01 1999-02-02 Smithkline Beecham Corporation gidB
US5916776A (en) 1997-08-27 1999-06-29 Sarnoff Corporation Amplification method for a polynucleotide
US6120985A (en) * 1997-10-31 2000-09-19 Bbi Bioseq, Inc. Pressure-enhanced extraction and purification
US6111096A (en) * 1997-10-31 2000-08-29 Bbi Bioseq, Inc. Nucleic acid isolation and purification
US5932451A (en) 1997-11-19 1999-08-03 Incyte Pharmaceuticals, Inc. Method for unbiased mRNA amplification
ES2287989T3 (es) * 1997-12-22 2007-12-16 Dsm Ip Assets B.V. Clonacion de expresion en hongos filamentosos.
US6387695B1 (en) 1997-12-23 2002-05-14 Merck & Co., Inc. DNA pharmaceutical formulations comprising citrate or triethanolamine and combinations thereof
US6265168B1 (en) 1998-10-06 2001-07-24 Transgenomic, Inc. Apparatus and method for separating and purifying polynucleotides
US6324479B1 (en) 1998-05-08 2001-11-27 Rosetta Impharmatics, Inc. Methods of determining protein activity levels using gene expression profiles
US6406921B1 (en) 1998-07-14 2002-06-18 Zyomyx, Incorporated Protein arrays for high-throughput screening
US6316193B1 (en) 1998-10-06 2001-11-13 Origene Technologies, Inc. Rapid-screen cDNA library panels
US6248535B1 (en) 1999-12-20 2001-06-19 University Of Southern California Method for isolation of RNA from formalin-fixed paraffin-embedded tissue specimens
WO2002081628A2 (en) 2001-04-05 2002-10-17 Ribozyme Pharmaceuticals, Incorporated Modulation of gene expression associated with inflammation proliferation and neurite outgrowth, using nucleic acid based technologies
WO2001071732A2 (en) 2000-03-24 2001-09-27 Qiagen Gmbh Porous ferro- or ferrimagnetic glass particles for isolating molecules
DE10031236A1 (de) * 2000-06-27 2002-01-10 Qiagen Gmbh Verwendung von Carbonsäuren und anderen Additiven in Kombination mit kationischen Verbindungen zur Stabilisierung von Nukleinsäuren in biologischen Materialien
JP3692395B2 (ja) * 2000-09-01 2005-09-07 独立行政法人農業・生物系特定産業技術研究機構 ベクターモノカリオンを用いた紫紋羽病菌に対するゲノムが２本鎖ｒｎａである糸状菌に寄生するウイルスを導入する新規方法
US20020086291A1 (en) 2000-11-29 2002-07-04 Hornby David P. Methods and reagents for analysis of rna structure and function
US20020139751A1 (en) * 2001-02-20 2002-10-03 Sheng Zhang Microchip electrospray device and column with affinity adsorbents and use of the same
US6509175B2 (en) 2001-04-11 2003-01-21 Incyte Genomics, Inc. cDNA libraries and methods for their production
JP2002345462A (ja) * 2001-05-24 2002-12-03 Fuji Photo Film Co Ltd 吸着バッファ及び核酸精製方法
US20020197611A1 (en) 2001-06-21 2002-12-26 Chagovetz Alexander Michael Method for real-time detection and quantification of nucleic acid sequences using fluorescent primers
DE60234464D1 (de) * 2001-11-28 2009-12-31 Applied Biosystems Llc Zusammensetzungen und Verfahren zur selektiven Nukleinsäureisolierung
ES2294287T5 (es) 2002-01-08 2015-10-27 F. Hoffmann-La Roche Ag Empleo de un material de sílice en una reacción de amplificación
WO2003100000A2 (en) * 2002-05-24 2003-12-04 Tularik Inc. Amplification and overexpression of oncogenes
EP1433858A1 (en) 2002-12-27 2004-06-30 Rengo Co., Ltd. Preparation method of insect cell extract solution for cell-free protein synthesis, the insect cell extract solution and cell-free synthesis method of protein using the insect cell extract solution
KR100501526B1 (ko) 2003-03-28 2005-07-18 한국기초과학지원연구원 어피니티 칼럼과 디엔에이자임을 이용한 고효율 알엔에이 제조방법
EP2295569A1 (en) * 2003-07-25 2011-03-16 Ambion, Inc. Methods and compositions for preparing rna from a fixed sample
US20050059024A1 (en) 2003-07-25 2005-03-17 Ambion, Inc. Methods and compositions for isolating small RNA molecules
US20050026153A1 (en) * 2003-07-31 2005-02-03 Iannotti Claudia A. Devices and methods for isolating RNA
WO2005012487A2 (en) 2003-08-01 2005-02-10 Invitrogen Corporation Compositions and methods for preparing short rna molecules and other nucleic acids
JP2007502420A (ja) * 2003-08-12 2007-02-08 マサチューセッツ インスティテュート オブ テクノロジー サンプル調製方法および装置
US20060233671A1 (en) 2003-09-19 2006-10-19 Beard Nigel P High density plate filler
AU2005250432B2 (en) * 2004-05-28 2011-09-15 Asuragen, Inc. Methods and compositions involving microRNA
US8312249B1 (en) 2008-10-10 2012-11-13 Apple Inc. Dynamic trampoline and structured code generation in a signed code environment
FR2957821B1 (fr) 2010-03-24 2014-08-29 Inst Francais Du Petrole Nouvelle zone de regeneration du catalyseur divisee en secteurs pour unites catalytiques regeneratives
CA2880136C (en) * 2012-09-03 2023-08-15 Qiagen Gmbh Method for isolating rna including small rna with high yield

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