ES2734807T3 - Inmunoensayo basado en partículas que usa un agente de unión específica a un analito pegilado - Google Patents
Inmunoensayo basado en partículas que usa un agente de unión específica a un analito pegilado Download PDFInfo
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- ES2734807T3 ES2734807T3 ES16754484T ES16754484T ES2734807T3 ES 2734807 T3 ES2734807 T3 ES 2734807T3 ES 16754484 T ES16754484 T ES 16754484T ES 16754484 T ES16754484 T ES 16754484T ES 2734807 T3 ES2734807 T3 ES 2734807T3
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- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
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- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
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- G01N33/54326—Magnetic particles
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Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
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EP15181763 | 2015-08-20 | ||
PCT/EP2016/069563 WO2017029346A1 (en) | 2015-08-20 | 2016-08-18 | Particle-based immunoassay using a pegylated analyte-specific binding agent |
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EP (1) | EP3338094B1 (zh) |
JP (2) | JP6979941B2 (zh) |
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CN (2) | CN117491623A (zh) |
BR (1) | BR112018001956A2 (zh) |
ES (1) | ES2734807T3 (zh) |
HK (1) | HK1248315A1 (zh) |
PL (1) | PL3338094T3 (zh) |
TR (1) | TR201909273T4 (zh) |
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Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP7068323B2 (ja) | 2017-02-02 | 2022-05-16 | エフ.ホフマン-ラ ロシュ アーゲー | 少なくとも2種のペグ化された分析物特異的結合剤を使用する免疫アッセイ |
EP3505933A1 (en) * | 2017-12-29 | 2019-07-03 | Blink AG | A microcapsule for detecting and/or quantitating an analyte in a sample |
US20220011301A1 (en) * | 2018-11-16 | 2022-01-13 | Roche Diagnostics Operations, Inc. | Streptavidin-coated solid phases with a member of a binding pair |
US20200199567A1 (en) * | 2018-12-20 | 2020-06-25 | Medimmune, Llc | Methods for selection and expansion of t cells expressing pd-1 |
US20200232979A1 (en) * | 2019-01-22 | 2020-07-23 | Mayo Foundation For Medical Education And Research | Microcapsules and Methods for Analyte Detection |
CN110018142B (zh) * | 2019-03-20 | 2021-10-22 | 西南交通大学 | 复合荧光基底、复合荧光基底的制备方法以及应用 |
CN113125740B (zh) * | 2021-05-17 | 2022-03-01 | 郑州安图生物工程股份有限公司 | 化学发光免疫分析技术中磁微粒活化效率的鉴定方法 |
Family Cites Families (52)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4275149A (en) | 1978-11-24 | 1981-06-23 | Syva Company | Macromolecular environment control in specific receptor assays |
US4318980A (en) | 1978-04-10 | 1982-03-09 | Miles Laboratories, Inc. | Heterogenous specific binding assay employing a cycling reactant as label |
US4816567A (en) | 1983-04-08 | 1989-03-28 | Genentech, Inc. | Recombinant immunoglobin preparations |
US5221605A (en) | 1984-10-31 | 1993-06-22 | Igen, Inc. | Luminescent metal chelate labels and means for detection |
US4737456A (en) | 1985-05-09 | 1988-04-12 | Syntex (U.S.A.) Inc. | Reducing interference in ligand-receptor binding assays |
US6548640B1 (en) | 1986-03-27 | 2003-04-15 | Btg International Limited | Altered antibodies |
EP0265519B1 (en) | 1986-04-30 | 1995-09-13 | IGEN, INC. (a California corporation) | Electrochemiluminescent assays |
US5591581A (en) | 1986-04-30 | 1997-01-07 | Igen, Inc. | Electrochemiluminescent rhenium moieties and methods for their use |
GB8823869D0 (en) | 1988-10-12 | 1988-11-16 | Medical Res Council | Production of antibodies |
DE68928492T2 (de) | 1988-11-03 | 1998-08-20 | Igen Inc | Elektrochemilumineszente reaktion unter verwendung eines von einem amin abgeleiteten reduktionsmittels |
EP0757252A3 (en) | 1988-11-03 | 1997-04-16 | Igen Inc | Electrochemiluminescent test procedure |
DE3915135A1 (de) | 1989-05-09 | 1990-11-15 | Boehringer Mannheim Gmbh | Verfahren zum nachweis spezifisch bindefaehiger substanzen in koerperfluessigkeiten |
DE3920358A1 (de) | 1989-06-22 | 1991-01-17 | Behringwerke Ag | Bispezifische und oligospezifische, mono- und oligovalente antikoerperkonstrukte, ihre herstellung und verwendung |
EP0463151B1 (en) | 1990-01-12 | 1996-06-12 | Cell Genesys, Inc. | Generation of xenogeneic antibodies |
US6150584A (en) | 1990-01-12 | 2000-11-21 | Abgenix, Inc. | Human antibodies derived from immunized xenomice |
US6075181A (en) | 1990-01-12 | 2000-06-13 | Abgenix, Inc. | Human antibodies derived from immunized xenomice |
US5625126A (en) | 1990-08-29 | 1997-04-29 | Genpharm International, Inc. | Transgenic non-human animals for producing heterologous antibodies |
US5545806A (en) | 1990-08-29 | 1996-08-13 | Genpharm International, Inc. | Ransgenic non-human animals for producing heterologous antibodies |
US5633425A (en) | 1990-08-29 | 1997-05-27 | Genpharm International, Inc. | Transgenic non-human animals capable of producing heterologous antibodies |
ES2108048T3 (es) | 1990-08-29 | 1997-12-16 | Genpharm Int | Produccion y utilizacion de animales inferiores transgenicos capaces de producir anticuerpos heterologos. |
US5661016A (en) | 1990-08-29 | 1997-08-26 | Genpharm International Inc. | Transgenic non-human animals capable of producing heterologous antibodies of various isotypes |
IL100867A (en) | 1991-02-06 | 1995-12-08 | Igen Inc | Method and device for improved luminescence testing |
GB9114948D0 (en) | 1991-07-11 | 1991-08-28 | Pfizer Ltd | Process for preparing sertraline intermediates |
ZA929351B (en) | 1991-12-11 | 1993-06-04 | Igen Inc | Electrochemiluminescent label for DNA assays. |
CA2172248C (en) | 1993-09-22 | 2003-12-30 | John Kenten | Self-sustained sequence replication electrochemiluminescent nucleic acid assay |
US5491968A (en) | 1994-03-21 | 1996-02-20 | Shouman; Ahmad R. | Combustion system and method for power generation |
US5786141A (en) | 1994-08-26 | 1998-07-28 | Bard; Allen J. | Electrogenerated chemiluminescence labels for analysis and/or referencing |
WO1996006946A1 (en) | 1994-08-26 | 1996-03-07 | Igen, Inc. | Biosensor for and method of electrogenerated chemiluminescent detection of nucleic acid adsorbed to a solid surface |
US5866434A (en) | 1994-12-08 | 1999-02-02 | Meso Scale Technology | Graphitic nanotubes in luminescence assays |
US5643713A (en) | 1995-06-07 | 1997-07-01 | Liang; Pam | Electrochemiluminescent monitoring of compounds |
US6852502B1 (en) | 1995-06-06 | 2005-02-08 | Bioveris Corporation | Electrochemiluminescent enzyme biosensors |
US6207369B1 (en) | 1995-03-10 | 2001-03-27 | Meso Scale Technologies, Llc | Multi-array, multi-specific electrochemiluminescence testing |
AU5665196A (en) | 1995-04-18 | 1996-11-07 | Igen, Inc. | Electrochemiluminescence of rare earth metal chelates |
CA2219361C (en) | 1995-04-27 | 2012-02-28 | Abgenix, Inc. | Human antibodies derived from immunized xenomice |
WO1996034096A1 (en) | 1995-04-28 | 1996-10-31 | Abgenix, Inc. | Human antibodies derived from immunized xenomice |
US5679519A (en) | 1995-05-09 | 1997-10-21 | Oprandy; John J. | Multi-label complex for enhanced sensitivity in electrochemiluminescence assay |
JP3784072B2 (ja) | 1995-06-07 | 2006-06-07 | イゲン,インコーポレイテッド | 電気化学発光酵素イムノアッセイ |
DE19637718A1 (de) | 1996-04-01 | 1997-10-02 | Boehringer Mannheim Gmbh | Rekombinante inaktive Core-Streptavidin Mutanten |
EP1500329B1 (en) | 1996-12-03 | 2012-03-21 | Amgen Fremont Inc. | Human antibodies that specifically bind human TNF alpha |
US6391571B1 (en) | 1997-04-01 | 2002-05-21 | Roche Diagnostics Gmbh | Recombinant inactive avidin mutants |
EP1034298B1 (en) | 1997-12-05 | 2011-11-02 | The Scripps Research Institute | Humanization of murine antibody |
WO2005075997A1 (ja) * | 2004-02-03 | 2005-08-18 | Asahi Kasei Kabushiki Kaisha | 磁気ビーズを用いた被検物質の検出方法 |
WO2010078376A2 (en) * | 2008-12-30 | 2010-07-08 | Ventana Medical Systems, Inc. | Fc-specific polymer-conjugated antibodies and their diagnostic use |
WO2011100669A2 (en) * | 2010-02-15 | 2011-08-18 | North Carolina State University Office Of Technology Transfer | Lable-free multiplexing bioassays using fluorescent conjugated polymers and barcoded nanoparticles |
WO2012028697A1 (en) | 2010-09-01 | 2012-03-08 | Eth Zürich, Institute Of Molecular Biology And Biophysics | Affinity purification system based on donor strand complementation |
US20140051070A1 (en) * | 2011-02-15 | 2014-02-20 | Kyowa Medex Co., Ltd. | Streptavidin-coupled magnetic particles and manufacturing method for same |
EP2541250A1 (en) | 2011-06-30 | 2013-01-02 | Koninklijke Philips Electronics N.V. | Molecular architecture on magnetic particles for affinity assays with low non-specific binding |
EP2594942A1 (en) * | 2011-11-16 | 2013-05-22 | Koninklijke Philips Electronics N.V. | Long rigid spacers to enhance binding kinetics in immunoassays |
JP6020914B2 (ja) * | 2012-04-10 | 2016-11-02 | パナソニックIpマネジメント株式会社 | ロジウムに結合できるペプチド |
JP6231263B2 (ja) * | 2012-07-17 | 2017-11-15 | 株式会社島津製作所 | アフィニティ支持体及びそれを用いた物質の捕捉方法 |
EP2816345A1 (en) * | 2013-06-19 | 2014-12-24 | Roche Diagniostics GmbH | Electrochemiluminescence method of detecting an analyte in a liquid sample and analysis system |
JP7068323B2 (ja) * | 2017-02-02 | 2022-05-16 | エフ.ホフマン-ラ ロシュ アーゲー | 少なくとも2種のペグ化された分析物特異的結合剤を使用する免疫アッセイ |
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CN117491623A (zh) | 2024-02-02 |
JP2018525637A (ja) | 2018-09-06 |
KR20180039163A (ko) | 2018-04-17 |
US20180172680A1 (en) | 2018-06-21 |
US11391733B2 (en) | 2022-07-19 |
KR102601323B1 (ko) | 2023-11-10 |
PL3338094T3 (pl) | 2019-09-30 |
EP3338094A1 (en) | 2018-06-27 |
CN107923911A (zh) | 2018-04-17 |
TR201909273T4 (tr) | 2019-07-22 |
JP6979941B2 (ja) | 2021-12-15 |
JP2021182010A (ja) | 2021-11-25 |
EP3338094B1 (en) | 2019-05-08 |
HK1248315A1 (zh) | 2018-10-12 |
WO2017029346A1 (en) | 2017-02-23 |
US20220317119A1 (en) | 2022-10-06 |
BR112018001956A2 (pt) | 2018-09-11 |
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