ES2664227T3 - Análisis celular de fluidos corporales - Google Patents

Análisis celular de fluidos corporales Download PDF

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Publication number
ES2664227T3
ES2664227T3 ES12862848.4T ES12862848T ES2664227T3 ES 2664227 T3 ES2664227 T3 ES 2664227T3 ES 12862848 T ES12862848 T ES 12862848T ES 2664227 T3 ES2664227 T3 ES 2664227T3
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Prior art keywords
fluid
cells
body fluid
fluorescent dye
dye
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ES12862848.4T
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English (en)
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Jiong Wu
Emily H. LIN
Jihping YANG
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Abbott Laboratories
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Abbott Laboratories
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5094—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for blood cell populations
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/01—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials specially adapted for biological cells, e.g. blood cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N2015/1006—Investigating individual particles for cytology
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/487—Physical analysis of biological material of liquid biological material
    • G01N33/49—Blood

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Organic Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Ecology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biophysics (AREA)
  • Toxicology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Dispersion Chemistry (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)

Abstract

Un método in vitro para analizar un fluido corporal que contiene células, en que dicho método comprende: teñir un fluido corporal que comprende menos de 100 células/μl y seleccionado entre líquido cefalorraquídeo, líquido peritoneal, líquido pericárdico, líquido pleural, líquido sinovial, orina, saliva, lágrimas, semen, líquido amniótico, esputo y líquido ascítico, con un colorante fluorescente, en el que el colorante fluorescente está presente en una cantidad entre 0,000001 y 0,5 % (p/v), penetra en una membrana celular y se une a un ácido nucleico para formar un complejo colorante en el interior de la célula; irradiar el fluido corporal teñido con energía procedente de una fuente de energía; medir una señal de fluorescencia emitida por el complejo colorante en el fluido corporal teñido; y diferenciar células con núcleos de células sin núcleos, basándose en la presencia o ausencia del colorante fluorescente.

Description

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Claims (1)

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ES12862848.4T 2011-12-27 2012-12-19 Análisis celular de fluidos corporales Active ES2664227T3 (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201161580623P 2011-12-27 2011-12-27
US201161580623P 2011-12-27
PCT/US2012/070696 WO2013101603A1 (en) 2011-12-27 2012-12-19 Cellular analysis of body fluids

Publications (1)

Publication Number Publication Date
ES2664227T3 true ES2664227T3 (es) 2018-04-18

Family

ID=48654915

Family Applications (2)

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ES18153846T Active ES2895391T3 (es) 2011-12-27 2012-12-19 Análisis celular de fluidos corporales
ES12862848.4T Active ES2664227T3 (es) 2011-12-27 2012-12-19 Análisis celular de fluidos corporales

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Application Number Title Priority Date Filing Date
ES18153846T Active ES2895391T3 (es) 2011-12-27 2012-12-19 Análisis celular de fluidos corporales

Country Status (5)

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US (3) US9970045B2 (es)
EP (2) EP3382035B1 (es)
CN (2) CN108279301A (es)
ES (2) ES2895391T3 (es)
WO (1) WO2013101603A1 (es)

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US8986944B2 (en) * 2001-10-11 2015-03-24 Aviva Biosciences Corporation Methods and compositions for separating rare cells from fluid samples
US8980568B2 (en) * 2001-10-11 2015-03-17 Aviva Biosciences Corporation Methods and compositions for detecting non-hematopoietic cells from a blood sample
CN101583722A (zh) * 2006-07-14 2009-11-18 阿维瓦生物科学股份有限公司 从生物学样品检测稀有细胞的方法和组合物
ES2895391T3 (es) * 2011-12-27 2022-02-21 Abbott Lab Análisis celular de fluidos corporales
WO2015168393A1 (en) * 2014-04-30 2015-11-05 Northwestern University Nanostructures for modulating intercellular communication and uses thereof
US10413565B2 (en) 2014-04-30 2019-09-17 Northwestern University Nanostructures for modulating intercellular communication and uses thereof
CN109963936A (zh) * 2016-09-08 2019-07-02 雅培实验室 自动体液分析
CN107686859B (zh) * 2017-07-18 2021-07-30 天津师范大学 一种适用于鱼类血细胞快速分类计数的方法及应用
JP7144511B2 (ja) * 2017-09-05 2022-09-29 ベックマン コールター， インコーポレイテッド 診療現場診断のための血液サンプルの収集および調製
KR20200127996A (ko) * 2018-03-01 2020-11-11 에코랍 유에스에이 인코퍼레이티드 수 중의 벤즈이미다졸-계 화합물을 측정하는 방법
CN114585895A (zh) * 2019-08-22 2022-06-03 迈克尔·伊安诺蒂 高通量分析和分选以及用于高通量分析和分选的采样接口和组件
WO2021077327A1 (zh) * 2019-10-23 2021-04-29 深圳迈瑞生物医疗电子股份有限公司 分析血液样本中红细胞方法及血液分析系统
CN113670696A (zh) * 2021-07-28 2021-11-19 上海睿钰生物科技有限公司 一种用于细胞染色的染色液及其配制方法
CN116067742B (zh) * 2023-03-29 2023-09-01 北京汉氏联合生物技术股份有限公司 CTCs染色试剂盒和染色方法

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Also Published As

Publication number Publication date
US10774359B2 (en) 2020-09-15
EP3382035B1 (en) 2021-08-11
US20210130866A1 (en) 2021-05-06
EP2798087A1 (en) 2014-11-05
US20130164740A1 (en) 2013-06-27
EP2798087B1 (en) 2018-03-07
EP2798087A4 (en) 2015-06-24
EP3382035A1 (en) 2018-10-03
WO2013101603A1 (en) 2013-07-04
ES2895391T3 (es) 2022-02-21
CN108279301A (zh) 2018-07-13
US20180334699A1 (en) 2018-11-22
CN104024437A (zh) 2014-09-03
US9970045B2 (en) 2018-05-15

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