ES2519042T3 - Sistema móvil de test rápido para análisis de ácido nucleico - Google Patents

Sistema móvil de test rápido para análisis de ácido nucleico Download PDF

Info

Publication number
ES2519042T3
ES2519042T3 ES08864313.5T ES08864313T ES2519042T3 ES 2519042 T3 ES2519042 T3 ES 2519042T3 ES 08864313 T ES08864313 T ES 08864313T ES 2519042 T3 ES2519042 T3 ES 2519042T3
Authority
ES
Spain
Prior art keywords
nucleic acid
amplification
test system
acid analysis
mobile
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
ES08864313.5T
Other languages
English (en)
Inventor
Timo Hillebrand
Claus Knippschild
Benjamin Jaschinsky
Elmara Graser
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
AJ Innuscreen GmbH
Original Assignee
AJ Innuscreen GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by AJ Innuscreen GmbH filed Critical AJ Innuscreen GmbH
Application granted granted Critical
Publication of ES2519042T3 publication Critical patent/ES2519042T3/es
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5023—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures with a sample being transported to, and subsequently stored in an absorbent for analysis
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/02—Adapting objects or devices to another
    • B01L2200/026—Fluid interfacing between devices or objects, e.g. connectors, inlet details
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/04—Exchange or ejection of cartridges, containers or reservoirs
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/10—Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0627—Sensor or part of a sensor is integrated
    • B01L2300/0636—Integrated biosensor, microarrays
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0825—Test strips
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/18—Means for temperature control
    • B01L2300/1805—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks
    • B01L2300/1822—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks using Peltier elements

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Clinical Laboratory Science (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Hematology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

Sistema móvil de test rápido para análisis de ácido nucleico en forma de un equipo manual a hacer funcionar de forma móvil, que durante el funcionamiento no necesita de ninguna fuente de tensión externa, que comprende un dispositivo para la amplificación e hibridización de ácidos nucleicos así como un kit de ensayo para la comprobación del suceso de amplificación, en donde el dispositivo comprende una cavidad de reacción para la realización de una amplificación de ácidos nucleicos mediante la tecnología de PCR rápido, uno o varios orificios de entrada o de salida para la cavidad de reacción, uno o varios bloques de muestra calefactables que están unidos con cuerpos miniaturizados de refrigeración y una posibilidad para la lectura del resultado, caracterizado porque: a) Los bloques de muestra calefactables están realizados de forma convexa y las superficies no calentadas son entre 1,4 y 1,9 veces más grandes que las superficies calentadas; b) La cavidad de reacción contiene una lámina de plástico con un grosor de lámina que es menor que 300 μm, y c) La comprobación del suceso de amplificación se realiza mediante una tira de ensayo de flujo lateral.

Description

imagen1
imagen2
imagen3
imagen4
imagen5
imagen6
imagen7
imagen8
imagen9

Claims (1)

  1. imagen1
    imagen2
ES08864313.5T 2007-12-20 2008-12-22 Sistema móvil de test rápido para análisis de ácido nucleico Active ES2519042T3 (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DE102007062441A DE102007062441A1 (de) 2007-12-20 2007-12-20 Mobiles Schnelltestsystem für die Nukleinsäureanalytik
DE102007062441 2007-12-22
PCT/EP2008/068197 WO2009080817A2 (de) 2007-12-20 2008-12-22 Mobiles schnelltestsystem für die nukleinsäureanalytik

Publications (1)

Publication Number Publication Date
ES2519042T3 true ES2519042T3 (es) 2014-11-06

Family

ID=40445408

Family Applications (1)

Application Number Title Priority Date Filing Date
ES08864313.5T Active ES2519042T3 (es) 2007-12-20 2008-12-22 Sistema móvil de test rápido para análisis de ácido nucleico

Country Status (7)

Country Link
US (2) US20110039261A1 (es)
EP (1) EP2227330B1 (es)
DE (1) DE102007062441A1 (es)
DK (1) DK2227330T3 (es)
ES (1) ES2519042T3 (es)
PL (1) PL2227330T3 (es)
WO (1) WO2009080817A2 (es)

Families Citing this family (40)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20090047673A1 (en) 2006-08-22 2009-02-19 Cary Robert B Miniaturized lateral flow device for rapid and sensitive detection of proteins or nucleic acids
US8980561B1 (en) 2006-08-22 2015-03-17 Los Alamos National Security, Llc. Nucleic acid detection system and method for detecting influenza
CA2723536C (en) 2008-05-05 2017-06-13 Los Alamos National Security, Llc Highly simplified lateral flow-based nucleic acid sample preparation and passive fluid flow control
GB0919159D0 (en) * 2009-11-02 2009-12-16 Sec Dep For Environment Food A Device and apparatus
DE102010003782B4 (de) 2010-04-08 2023-09-28 Ist Innuscreen Gmbh Vorrichtung zum Nachweis von Nukleinsäuren
CA2814423C (en) 2010-10-15 2019-01-15 Atomo Diagnostics Pty Limited Sampling assembly
CN102087295B (zh) * 2011-01-24 2013-04-03 杭州安旭科技有限公司 一种快速检测装置
CA2853615C (en) 2011-04-20 2016-07-05 Mesa Tech International, Inc. Integrated device for nucleic acid detection and identification
WO2013113072A1 (en) 2012-02-03 2013-08-08 Axxin Pty Ltd Nucleic acid amplification and detection apparatus and method
WO2014000037A1 (en) * 2012-06-26 2014-01-03 Axxin Pty Ltd Nucleic acid amplification and detection kit
BE1020816A5 (fr) * 2012-07-04 2014-05-06 Coris Bioconcept Sprl Procede et dispositif pour la detection rapide de sequences nucleotidiques amplifiees.
JP6272895B2 (ja) 2012-12-03 2018-01-31 ザ セクレタリー オブ ステイト フォー エンバイロンメント，フード アンド ルーラル アフェアーズ デバイスおよび装置
US9714447B2 (en) * 2013-08-19 2017-07-25 General Electric Company Detection of nucleic acid amplification in a porous substrate
AU2014351425B2 (en) * 2013-11-21 2018-11-15 Atomo Diagnostics Limited Fluid control in integrated testing devices
US10195610B2 (en) 2014-03-10 2019-02-05 Click Diagnostics, Inc. Cartridge-based thermocycler
US9506908B2 (en) 2014-10-06 2016-11-29 Alveo Technologies, Inc. System for detection of analytes
US10627358B2 (en) 2014-10-06 2020-04-21 Alveo Technologies, Inc. Method for detection of analytes
US9921182B2 (en) 2014-10-06 2018-03-20 ALVEO Technologies Inc. System and method for detection of mercury
US10196678B2 (en) 2014-10-06 2019-02-05 ALVEO Technologies Inc. System and method for detection of nucleic acids
US10352899B2 (en) 2014-10-06 2019-07-16 ALVEO Technologies Inc. System and method for detection of silver
ES2809600T3 (es) 2014-11-14 2021-03-04 Axxin Pty Ltd Conjunto de recogida y almacenamiento de muestras biológicas
WO2016109691A1 (en) 2014-12-31 2016-07-07 Boris Andreyev Devices and methods for molecular diagnostic testing
EP3325156A4 (en) 2015-07-17 2019-01-16 Axxin Pty Ltd DIAGNOSTIC TEST ASSEMBLY, APPARATUS, METHOD
GB201513128D0 (en) * 2015-07-24 2015-09-09 Sense Biodetection Ltd Nucleic acid detection method
DE102015217785A1 (de) 2015-09-17 2017-03-23 Robert Bosch Gmbh Trockenchemisch gesteuerte Vorrichtung zur Analyse einer Probe biologischen Materials
WO2017185067A1 (en) 2016-04-22 2017-10-26 Click Diagnostics, Inc. Printed circuit board heater for an amplification module
WO2017197040A1 (en) 2016-05-11 2017-11-16 Click Diagnostics, Inc. Devices and methods for nucleic acid extraction
CN110325652A (zh) 2016-06-29 2019-10-11 易捷仪器诊断股份有限公司 使用流动池检测分子的装置和方法
PL3293748T3 (pl) 2016-09-09 2020-11-16 Microelettrica Scientifica S.P.A. Ulepszony stycznik do zastosowań przy przełączeniach wysokoprądowych
JP7146743B2 (ja) 2016-09-23 2022-10-04 アルヴェオ テクノロジーズ インコーポレイテッド 分析種を検出するための方法および組成物
CN107513494A (zh) * 2017-08-17 2017-12-26 湖北伽诺美生物科技有限公司 一种核酸检测卡及其使用方法
US11709175B2 (en) 2017-09-27 2023-07-25 Axxin Pty Ltd Diagnostic test system and method utilizing a closure/sample dispensing mechanism to dispense a sample subvolume for testing
AU2018364741B2 (en) 2017-11-09 2021-03-25 Visby Medical, Inc. Portable molecular diagnostic device and methods for the detection of target viruses
CN211955507U (zh) * 2019-02-25 2020-11-17 上海快灵生物科技有限公司 一种生化反应试纸条管及试剂盒
WO2021138544A1 (en) 2020-01-03 2021-07-08 Visby Medical, Inc. Devices and methods for antibiotic susceptibility testing
US11494571B2 (en) 2020-07-22 2022-11-08 Donald Channing Cooper Computer vision method for improved automated image capture and analysis of rapid diagnostic test devices
CN112891986B (zh) * 2021-01-30 2022-05-03 哈尔滨工业大学 一种集成封闭式核酸自动提取装置
DE102021110828A1 (de) * 2021-04-28 2022-11-03 Innovative Sensor Technology Ist Ag System, Verfahren und Verbrauchsprodukt für ein chemisches, bio- oder immunchemisches Nachweisverfahren
DE102021125054A1 (de) 2021-09-28 2023-03-30 Alfred-Wegener-Institut, Helmholtz-Zentrum für Polar- und Meeresforschung, Stiftung des öffentlichen Rechts Trockenfutter zur Ernährung von sessilen Filtrierern und Anwendung
CN114015552B (zh) * 2021-11-04 2022-06-07 贵州医科大学附属医院 一种用于鉴定人骨髓间充质干细胞的试剂盒

Family Cites Families (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4622822A (en) * 1984-05-07 1986-11-18 Shlomo Beitner Peltier thermoelectric element mounting
US6524532B1 (en) * 1995-06-20 2003-02-25 The Regents Of The University Of California Microfabricated sleeve devices for chemical reactions
US5589136A (en) * 1995-06-20 1996-12-31 Regents Of The University Of California Silicon-based sleeve devices for chemical reactions
EP1000661A1 (en) 1998-10-29 2000-05-17 Hans-Knöll-Institut für Naturstoff-Forschung e.v. Ultrathin-walled multiwell plate for heat block thermocycling
US6692700B2 (en) * 2001-02-14 2004-02-17 Handylab, Inc. Heat-reduction methods and systems related to microfluidic devices
US20030207273A1 (en) * 2002-03-20 2003-11-06 Betty Wu Polynucleotide primers and probes for rapid detection of Group B streptococcal (GBS)
CA2513880A1 (en) * 2003-01-21 2004-08-05 Micronics Inc. Method and system for microfluidic manipulation, amplification and analysis of fluids, for example, bacteria assays and antiglobulin testing
ES2363501T3 (es) * 2003-05-07 2011-08-05 Coris Bioconcept Sprl Dispositivo oligocromático de un paso y procedimiento de uso.
JP2008500820A (ja) * 2004-04-07 2008-01-17 アクセス バイオ， アイエヌシー． 核酸検出システム
WO2007096702A2 (en) * 2005-07-25 2007-08-30 Asm Scientific, Inc. Methods for multiplexing recombinase polymerase amplification
US7569382B2 (en) * 2005-10-05 2009-08-04 Instantlabs Medical Diagnostic Corp. Disposable reactor module and detection system
JP5178528B2 (ja) * 2005-12-29 2013-04-10 アボット ポイント オブ ケア インコーポレイテッド 分子診断用増幅システムおよび方法
WO2007092713A2 (en) * 2006-02-02 2007-08-16 Trustees Of The University Of Pennsylvania Microfluidic system and method for analysis of gene expression in cell-containing samples and detection of disease
FR2897930B1 (fr) 2006-02-28 2008-05-16 Commissariat Energie Atomique Echangeur thermique a plaques incluant un dispositif d'evaluation de son etat d'encrassement
AU2007235649A1 (en) * 2006-04-10 2007-10-18 3M Innovative Properties Company Nucleic acid detection using lateral flow methods
WO2008076395A2 (en) * 2006-12-14 2008-06-26 The Trustees Of The University Of Pennsylvania Mechanically actuated diagnostic device
DE102007029772B4 (de) * 2007-06-22 2011-12-08 Aj Innuscreen Gmbh Verfahren und Schnelltest zum Nachweis spezifischer Nukleinsäuresequenzen

Also Published As

Publication number Publication date
US20130130257A1 (en) 2013-05-23
WO2009080817A2 (de) 2009-07-02
EP2227330A2 (de) 2010-09-15
US9260750B2 (en) 2016-02-16
EP2227330B1 (de) 2014-07-30
US20110039261A1 (en) 2011-02-17
DK2227330T3 (da) 2014-11-10
PL2227330T3 (pl) 2015-02-27
WO2009080817A3 (de) 2009-10-15
WO2009080817A8 (de) 2010-11-04
DE102007062441A1 (de) 2009-06-25

Similar Documents

Publication Publication Date Title
ES2519042T3 (es) Sistema móvil de test rápido para análisis de ácido nucleico
ES2650950T3 (es) Un método para filtrar una muestra biológica
WO2017181135A3 (en) METHODS, SYSTEMS AND KITS FOR IN PEN ASSAYS
ES2495994T3 (es) Accionadores de placas paralelas electrostáticas cuyos elementos móviles se accionan solamente mediante fuerza electrostática y procedimientos útiles en conjunción con los mismos
ES2547320T3 (es) Cinta y método para indicar impactos contundentes
ATE555216T1 (de) Umgebungstemperaturstabile kits für die molekulare diagnostik
CL2013000813A1 (es) Dispositivo inmunocromatográfico que comprende una membrana que tiene un anticuerpo capaz de unirse a una proteina z-aat presente en una muestra de un portador del gen piz; metodo para determinar un portador del gen piz; metodo para diagnosticar una afección o enfermedad asociada con la deficiencia de aat; kits de inmunocromatografia
ATE544063T1 (de) Nanoporenvorrichtung und verfahren zur nukleinsäureanalyse
BR112012019396A2 (pt) método e kit para amplificar um ácido nucleico.
CL2017002101A1 (es) Dispositivo, plataforma y ensayo para evaluación de células
CO6640223A2 (es) Sistemas y dispositivos para análisis de muestras
Li et al. The construction of an interfacial valve-based microfluidic chip for thermotaxis evaluation of human sperm
HN2012001269A (es) Dispositivo portatil de monitorizacion de la coagulacion y metodo de evaluacion de la respuesta de coagulacion.
NO20084083L (no) Anordning og fremgangsmate for detektering av fluorescensmerkede biologiske komponenter
WO2019075476A3 (en) Methods, systems and kits for in-pen assays
CL2016001226A1 (es) Método de reparación o modificación de una superficie de una membrana osmótica, poniendo en contacto la membrana con una sustancia seleccionada según el defecto o característica que se desea modificar, y que comprende biguanida polimérica; y sistema para mejorar el rendimiento de una membrana usando dicho método.
CR20130657A (es) Ensayo de lisiloxidasa-homólogo 2 y métodos de uso del mismo
ITUB20161187A1 (it) Sistema e procedimento per l’associazione dei risultati di analisi eseguite su campioni biologici, in particolare campioni biologici sottoposti ad indagini cliniche, con variabili pre-analitiche a cui detti campioni sono esposti.
AR055758A1 (es) Tanque para dispositivo optico para analisis de sangre, aparato de analisis equipado con tal tanque
FR2911353B1 (fr) Procede de cloisonnement et/ou contre-cloisonnement avec chauffage et/ou refroidissement integre
CY1120220T1 (el) Μεθοδοι και κυτια για προσδιορισμο της καταστασης λοιμωξης απο φυματιωση
AR105379A1 (es) Dispositivo para detectar proteínas mal plegadas y sus métodos de uso
BR112013029786A2 (pt) processo e disposição para detecção de células em uma amostra de líquido
EA201300009A1 (ru) Точные интегрированные электродные чипы для микроанализа и способ использования в системах с возбуждением электрохемилюминесценции горячими электронами
AR091150A1 (es) Proceso para preparar una pluralidad de pellets de adhesivo