WO2008123180A1 - 標的物質の検出方法、並びに、これに用いるタグ、ｄｎａ、ベクター、プローブ及び検出キット - Google Patents

標的物質の検出方法、並びに、これに用いるタグ、ｄｎａ、ベクター、プローブ及び検出キット Download PDF

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Publication number
WO2008123180A1
WO2008123180A1 PCT/JP2008/055399 JP2008055399W WO2008123180A1 WO 2008123180 A1 WO2008123180 A1 WO 2008123180A1 JP 2008055399 W JP2008055399 W JP 2008055399W WO 2008123180 A1 WO2008123180 A1 WO 2008123180A1
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WO
WIPO (PCT)
Prior art keywords
target substance
tag
probe
detection
dna
Prior art date
Application number
PCT/JP2008/055399
Other languages
English (en)
French (fr)
Inventor
Hirokazu Tamamura
Hiroshi Tsutsumi
Original Assignee
National University Corporation Tokyo Medical And Dental University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by National University Corporation Tokyo Medical And Dental University filed Critical National University Corporation Tokyo Medical And Dental University
Priority to JP2009509091A priority Critical patent/JP5306995B2/ja
Publication of WO2008123180A1 publication Critical patent/WO2008123180A1/ja
Priority to US12/586,425 priority patent/US8445291B2/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/13—Tracers or tags

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Pathology (AREA)
  • Hematology (AREA)
  • Organic Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Biomedical Technology (AREA)
  • Analytical Chemistry (AREA)
  • Urology & Nephrology (AREA)
  • Food Science & Technology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Cell Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Optics & Photonics (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

　標的物質の機能への影響を低減しつつ、標的物質の発現を任意の時間で可視化することができ、様々な励起・蛍光波長を有する蛍光色素を利用することができ、染色作業が容易な、標的物質の検出方法を提供すること。また、この検出方法に好適に用いることができるタグ、ＤＮＡ、ベクター、プローブ及び検出キットを提供することを目的とする。 　（ａ）αヘリックス構造を形成するポリペプチドからなり、標的物質に結合されたタグ、及び、（ｂ）蛍光色素が結合された化合物からなるプローブ、を接触させる工程と、蛍光色素が発する蛍光を測定する工程と、を含み、蛍光色素は、前記タグのαヘリックス構造及びプローブの化合物の結合によって、蛍光スペクトルの変化が誘起されることを特徴とする標的物質の検出方法、並びに、これに用いるタグ、ＤＮＡ、ベクター、プローブ及び検出キットである。  
PCT/JP2008/055399 2007-03-23 2008-03-24 標的物質の検出方法、並びに、これに用いるタグ、ｄｎａ、ベクター、プローブ及び検出キット WO2008123180A1 (ja)

Priority Applications (2)

Application Number Priority Date Filing Date Title
JP2009509091A JP5306995B2 (ja) 2007-03-23 2008-03-24 標的物質の検出方法、並びに、これに用いるタグ、ｄｎａ、ベクター、プローブ及び検出キット
US12/586,425 US8445291B2 (en) 2007-03-23 2009-09-22 Method for detecting target substance, and tag, DNA, vector, probe and detection kit for use therewith

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2007-077916 2007-03-23
JP2007077916 2007-03-23

Related Child Applications (1)

Application Number Title Priority Date Filing Date
US12/586,425 Continuation US8445291B2 (en) 2007-03-23 2009-09-22 Method for detecting target substance, and tag, DNA, vector, probe and detection kit for use therewith

Publications (1)

Publication Number Publication Date
WO2008123180A1 true WO2008123180A1 (ja) 2008-10-16

Family

ID=39830677

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2008/055399 WO2008123180A1 (ja) 2007-03-23 2008-03-24 標的物質の検出方法、並びに、これに用いるタグ、ｄｎａ、ベクター、プローブ及び検出キット

Country Status (3)

Country Link
US (1) US8445291B2 (ja)
JP (1) JP5306995B2 (ja)
WO (1) WO2008123180A1 (ja)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2011099844A (ja) * 2009-06-10 2011-05-19 Fujifilm Corp 抗原検出方法及び抗原検出装置
CN106896093A (zh) * 2017-03-01 2017-06-27 中南大学 一种蛋白质结构域与线性生物大分子相互作用的测定方法
JP2019526055A (ja) * 2016-08-01 2019-09-12 コグノプティックス， インコーポレイテッド 眼組織におけるタウタンパク質を検出するためのシステムおよび方法
WO2020027234A1 (ja) * 2018-08-02 2020-02-06 キヤノンメディカルシステムズ株式会社 分析方法、試薬キット及び分析装置

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB201714478D0 (en) 2017-09-08 2017-10-25 Univ Bristol Sensor
US20220128469A1 (en) * 2020-10-27 2022-04-28 Quantum-Si Incorporated Single molecule detection system using photobleaching information

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
CHEN I. ET AL.: "Site-specific labeling of proteins with small molecules in live cells", CURRENT OPINION IN BIOTECHNOLOGY, vol. 16, no. 1, 2005, pages 35 - 40, XP004756194 *
OBATAYA I. ET AL.: "Design and Synthesis of 3alpha-Helix Peptides Forming a Cavity for a Fluorescent Ligand", BIOPOLYMERES, vol. 59, 2001, pages 65 - 71 *
OJIDA A. ET AL.: "Oligo-Asp Tag/An(II) Complex Probe as a New Pair for Labeling and Fluorescence Imaging of Proteins", J. AM. CHEM. SOC., vol. 128, 2006, pages 10452 - 10459 *

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2011099844A (ja) * 2009-06-10 2011-05-19 Fujifilm Corp 抗原検出方法及び抗原検出装置
JP2019526055A (ja) * 2016-08-01 2019-09-12 コグノプティックス， インコーポレイテッド 眼組織におけるタウタンパク質を検出するためのシステムおよび方法
CN106896093A (zh) * 2017-03-01 2017-06-27 中南大学 一种蛋白质结构域与线性生物大分子相互作用的测定方法
WO2020027234A1 (ja) * 2018-08-02 2020-02-06 キヤノンメディカルシステムズ株式会社 分析方法、試薬キット及び分析装置

Also Published As

Publication number Publication date
US20100075434A1 (en) 2010-03-25
JPWO2008123180A1 (ja) 2010-07-15
JP5306995B2 (ja) 2013-10-02
US8445291B2 (en) 2013-05-21

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