ES2651483T3 - Método para preparar la forma activa de la proteína de fusión TNRF-Fc - Google Patents
Método para preparar la forma activa de la proteína de fusión TNRF-Fc Download PDFInfo
- Publication number
- ES2651483T3 ES2651483T3 ES12823797.1T ES12823797T ES2651483T3 ES 2651483 T3 ES2651483 T3 ES 2651483T3 ES 12823797 T ES12823797 T ES 12823797T ES 2651483 T3 ES2651483 T3 ES 2651483T3
- Authority
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- Prior art keywords
- tnfr
- buffer
- fusion protein
- column
- protein
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Links
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- PMZURENOXWZQFD-UHFFFAOYSA-L Sodium Sulfate Chemical compound [Na+].[Na+].[O-]S([O-])(=O)=O PMZURENOXWZQFD-UHFFFAOYSA-L 0.000 claims abstract description 34
- 229910052938 sodium sulfate Inorganic materials 0.000 claims abstract description 34
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- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 claims abstract description 32
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- RYFMWSXOAZQYPI-UHFFFAOYSA-K trisodium phosphate Chemical compound [Na+].[Na+].[Na+].[O-]P([O-])([O-])=O RYFMWSXOAZQYPI-UHFFFAOYSA-K 0.000 claims abstract description 30
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
- C07K14/70578—NGF-receptor/TNF-receptor superfamily, e.g. CD27, CD30, CD40, CD95
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K19/00—Hybrid peptides, i.e. peptides covalently bound to nucleic acids, or non-covalently bound protein-protein complexes
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
- C07K1/18—Ion-exchange chromatography
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
- C07K1/20—Partition-, reverse-phase or hydrophobic interaction chromatography
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
- C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
- C07K14/715—Receptors; Cell surface antigens; Cell surface determinants for cytokines; for lymphokines; for interferons
- C07K14/7151—Receptors; Cell surface antigens; Cell surface determinants for cytokines; for lymphokines; for interferons for tumor necrosis factor [TNF], for lymphotoxin [LT]
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/30—Non-immunoglobulin-derived peptide or protein having an immunoglobulin constant or Fc region, or a fragment thereof, attached thereto
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Biochemistry (AREA)
- Medicinal Chemistry (AREA)
- Genetics & Genomics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Immunology (AREA)
- Gastroenterology & Hepatology (AREA)
- Toxicology (AREA)
- Zoology (AREA)
- Cell Biology (AREA)
- Analytical Chemistry (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR20110081854 | 2011-08-17 | ||
| KR20110081854 | 2011-08-17 | ||
| PCT/KR2012/006564 WO2013025079A1 (en) | 2011-08-17 | 2012-08-17 | Method for preparing active form of tnfr-fc fusion protein |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ES2651483T3 true ES2651483T3 (es) | 2018-01-26 |
Family
ID=47715279
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES12823797.1T Active ES2651483T3 (es) | 2011-08-17 | 2012-08-17 | Método para preparar la forma activa de la proteína de fusión TNRF-Fc |
Country Status (6)
| Country | Link |
|---|---|
| US (1) | US9279014B2 (https=) |
| EP (1) | EP2753634B1 (https=) |
| JP (2) | JP6223338B2 (https=) |
| KR (1) | KR101454316B1 (https=) |
| ES (1) | ES2651483T3 (https=) |
| WO (1) | WO2013025079A1 (https=) |
Families Citing this family (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| HK1200718A1 (en) | 2011-10-18 | 2015-08-14 | 科荣生生物科学公司 | Etanercept formulations stabilized with sodium chloride |
| JP2015513406A (ja) * | 2012-03-12 | 2015-05-14 | ハンファ ケミカル コーポレーション | 改変グルタミン合成酵素をコードするポリヌクレオチドを含む発現ベクターおよびこれを用いた標的タンパク質の製造方法 |
| EA031324B1 (ru) * | 2012-09-11 | 2018-12-28 | Кохерус Байосайенсис, Инк. | Правильно свернутый этанерцепт с высокой чистотой и высоким уровнем выхода |
| EP3594231A1 (en) | 2013-02-13 | 2020-01-15 | Laboratoire Français du Fractionnement et des Biotechnologies | Highly galactosylated anti-tnf-alpha antibodies and uses thereof |
| AU2015273049B2 (en) * | 2014-06-13 | 2019-11-14 | Lupin Limited | Process for the purification of TNFR:Fc fusion protein |
| ES2733298T3 (es) | 2014-07-18 | 2019-11-28 | Sandoz Ag | Cuantificación de TNFR2:Fc plegado erróneamente |
| EP3241849A4 (en) * | 2014-12-31 | 2018-06-20 | LG Chem, Ltd. | Method for producing tnfr-fc fusion protein containing target content of impurities |
| KR20170138426A (ko) * | 2015-03-13 | 2017-12-15 | 삼성바이오에피스 주식회사 | 항-tnf-알파 폴리펩티드 조성물 및 그 용도 |
| US11952399B2 (en) * | 2018-12-18 | 2024-04-09 | Amgen Inc. | Methods for purifying proteins |
| US12411064B2 (en) | 2020-05-08 | 2025-09-09 | Waters Technologies Corporation | Methods for heat-assisted enzyme digestion |
| CN115552215A (zh) | 2020-05-08 | 2022-12-30 | 沃特世科技公司 | 用于热辅助酶消化的方法 |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| TR200504220T2 (tr) * | 1998-12-17 | 2007-04-24 | Biogen Idec Ma Inc. | Aktif limfotoksin-beta reseptör imunoglobülin şimeAktif limfotoksin-beta reseptör imunoglobülin şimerik proteinlerinin yüksek düzey ifadesi ve saflaştrik proteinlerinin yüksek düzey ifadesi ve saflaştırılması için bir yöntem.ırılması için bir yöntem. |
| US7294481B1 (en) | 1999-01-05 | 2007-11-13 | Immunex Corporation | Method for producing recombinant proteins |
| PT1946776T (pt) * | 2002-02-27 | 2017-03-17 | Immunex Corp | Composição de tnfr-fc estabilizada compreendendo arginina |
| DE602004026343D1 (de) * | 2003-10-24 | 2010-05-12 | Amgen Inc | Verfahren zur aufreinigung von proteinen in einer durchflussfraktion aus der chromatographie mit hydrophoben wechselwirkungen |
| US7781395B2 (en) * | 2004-01-30 | 2010-08-24 | Immunex Corporation | Process for purifying proteins |
| US20110129468A1 (en) * | 2008-02-29 | 2011-06-02 | Biogen Idec Ma Inc. | Purified immunoglobulin fusion proteins and methods of their purification |
| CN102675465A (zh) * | 2011-03-09 | 2012-09-19 | 上海赛金生物医药有限公司 | 重组人ⅱ型肿瘤坏死因子受体-抗体融合蛋白的纯化方法 |
| WO2012176158A1 (en) * | 2011-06-24 | 2012-12-27 | Dr. Reddy's Laboratories Limited | Purification of chimeric protein |
-
2012
- 2012-08-17 KR KR1020120090341A patent/KR101454316B1/ko not_active Expired - Fee Related
- 2012-08-17 JP JP2014525943A patent/JP6223338B2/ja not_active Expired - Fee Related
- 2012-08-17 EP EP12823797.1A patent/EP2753634B1/en active Active
- 2012-08-17 ES ES12823797.1T patent/ES2651483T3/es active Active
- 2012-08-17 WO PCT/KR2012/006564 patent/WO2013025079A1/en not_active Ceased
- 2012-08-17 US US14/239,216 patent/US9279014B2/en active Active
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2016
- 2016-05-26 JP JP2016105574A patent/JP2016168049A/ja active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| EP2753634A4 (en) | 2015-05-20 |
| JP2014521364A (ja) | 2014-08-28 |
| JP2016168049A (ja) | 2016-09-23 |
| EP2753634B1 (en) | 2017-11-01 |
| KR101454316B1 (ko) | 2014-10-27 |
| US9279014B2 (en) | 2016-03-08 |
| WO2013025079A1 (en) | 2013-02-21 |
| US20140316114A1 (en) | 2014-10-23 |
| KR20130020644A (ko) | 2013-02-27 |
| EP2753634A1 (en) | 2014-07-16 |
| JP6223338B2 (ja) | 2017-11-01 |
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