ES2651483T3 - Método para preparar la forma activa de la proteína de fusión TNRF-Fc - Google Patents
Método para preparar la forma activa de la proteína de fusión TNRF-Fc Download PDFInfo
- Publication number
- ES2651483T3 ES2651483T3 ES12823797.1T ES12823797T ES2651483T3 ES 2651483 T3 ES2651483 T3 ES 2651483T3 ES 12823797 T ES12823797 T ES 12823797T ES 2651483 T3 ES2651483 T3 ES 2651483T3
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- Prior art keywords
- tnfr
- buffer
- fusion protein
- column
- protein
- Prior art date
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Links
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- 102000037865 fusion proteins Human genes 0.000 title claims abstract description 40
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- 102000003298 tumor necrosis factor receptor Human genes 0.000 claims abstract description 39
- PMZURENOXWZQFD-UHFFFAOYSA-L Sodium Sulfate Chemical compound [Na+].[Na+].[O-]S([O-])(=O)=O PMZURENOXWZQFD-UHFFFAOYSA-L 0.000 claims abstract description 34
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- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 claims abstract description 32
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- 229910000162 sodium phosphate Inorganic materials 0.000 claims abstract description 30
- RYFMWSXOAZQYPI-UHFFFAOYSA-K trisodium phosphate Chemical compound [Na+].[Na+].[Na+].[O-]P([O-])([O-])=O RYFMWSXOAZQYPI-UHFFFAOYSA-K 0.000 claims abstract description 30
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
- C07K14/70578—NGF-receptor/TNF-receptor superfamily, e.g. CD27, CD30, CD40, CD95
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K19/00—Hybrid peptides, i.e. peptides covalently bound to nucleic acids, or non-covalently bound protein-protein complexes
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
- C07K1/18—Ion-exchange chromatography
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
- C07K1/20—Partition-, reverse-phase or hydrophobic interaction chromatography
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
- C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
- C07K14/715—Receptors; Cell surface antigens; Cell surface determinants for cytokines; for lymphokines; for interferons
- C07K14/7151—Receptors; Cell surface antigens; Cell surface determinants for cytokines; for lymphokines; for interferons for tumor necrosis factor [TNF], for lymphotoxin [LT]
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/30—Non-immunoglobulin-derived peptide or protein having an immunoglobulin constant or Fc region, or a fragment thereof, attached thereto
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Biochemistry (AREA)
- Medicinal Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Biophysics (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Cell Biology (AREA)
- Immunology (AREA)
- Toxicology (AREA)
- Zoology (AREA)
- Gastroenterology & Hepatology (AREA)
- Analytical Chemistry (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR20110081854 | 2011-08-17 | ||
| KR20110081854 | 2011-08-17 | ||
| PCT/KR2012/006564 WO2013025079A1 (en) | 2011-08-17 | 2012-08-17 | Method for preparing active form of tnfr-fc fusion protein |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ES2651483T3 true ES2651483T3 (es) | 2018-01-26 |
Family
ID=47715279
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES12823797.1T Active ES2651483T3 (es) | 2011-08-17 | 2012-08-17 | Método para preparar la forma activa de la proteína de fusión TNRF-Fc |
Country Status (6)
| Country | Link |
|---|---|
| US (1) | US9279014B2 (https=) |
| EP (1) | EP2753634B1 (https=) |
| JP (2) | JP6223338B2 (https=) |
| KR (1) | KR101454316B1 (https=) |
| ES (1) | ES2651483T3 (https=) |
| WO (1) | WO2013025079A1 (https=) |
Families Citing this family (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| BR112014009146A8 (pt) | 2011-10-18 | 2017-06-20 | Coherus Biosciences Inc | formulações de etanercepte estabilizadas com aminoácidos |
| US9567577B2 (en) * | 2012-03-12 | 2017-02-14 | Ares Trading S.A. | Expression vector comprising a polynucleotide encoding a modified glutamine synthetase and a method for preparing a target protein employing the same |
| SI2895188T1 (en) | 2012-09-11 | 2018-05-31 | Coherus Biosciences, Inc. | CORRECTED QUALITY ETHANERCEPT WITH HIGH QUALITY AND EXCELLENT EXPERIENCE |
| EP3594230A1 (en) | 2013-02-13 | 2020-01-15 | Laboratoire Français du Fractionnement et des Biotechnologies | Highly galactosylated anti-tnf-alpha antibodies and uses thereof |
| MX2016016318A (es) * | 2014-06-13 | 2017-06-12 | Lupin Atlantis Holdings Sa | Proceso para la purificacion de la proteina de fusion. |
| ES2733298T3 (es) | 2014-07-18 | 2019-11-28 | Sandoz Ag | Cuantificación de TNFR2:Fc plegado erróneamente |
| EP3241849A4 (en) | 2014-12-31 | 2018-06-20 | LG Chem, Ltd. | Method for producing tnfr-fc fusion protein containing target content of impurities |
| EP3268042B1 (en) * | 2015-03-13 | 2024-11-27 | Samsung Bioepis Co., Ltd. | Anti-tnf-alpha polypeptide composition and use thereof |
| US11952399B2 (en) * | 2018-12-18 | 2024-04-09 | Amgen Inc. | Methods for purifying proteins |
| CN115552215A (zh) | 2020-05-08 | 2022-12-30 | 沃特世科技公司 | 用于热辅助酶消化的方法 |
| US12411064B2 (en) | 2020-05-08 | 2025-09-09 | Waters Technologies Corporation | Methods for heat-assisted enzyme digestion |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| TR200504220T2 (tr) | 1998-12-17 | 2007-04-24 | Biogen Idec Ma Inc. | Aktif limfotoksin-beta reseptör imunoglobülin şimeAktif limfotoksin-beta reseptör imunoglobülin şimerik proteinlerinin yüksek düzey ifadesi ve saflaştrik proteinlerinin yüksek düzey ifadesi ve saflaştırılması için bir yöntem.ırılması için bir yöntem. |
| US7294481B1 (en) | 1999-01-05 | 2007-11-13 | Immunex Corporation | Method for producing recombinant proteins |
| SI1478394T1 (sl) | 2002-02-27 | 2008-12-31 | Immunex Corp | STABILIZIRAN TNFR-Fc SESTAVEK Z ARGININOM |
| US7427659B2 (en) * | 2003-10-24 | 2008-09-23 | Amgen Inc. | Process for purifying proteins in a hydrophobic interaction chromatography flow-through fraction |
| EP1711512B1 (en) | 2004-01-30 | 2012-01-11 | Amgen Inc. | Process for purifying proteins |
| BRPI0908270A2 (pt) | 2008-02-29 | 2019-09-10 | Biogen Idec Inc | proteínas de fusão de imunoglobulinas purificadas e método para a sua purificação |
| CN102675465A (zh) * | 2011-03-09 | 2012-09-19 | 上海赛金生物医药有限公司 | 重组人ⅱ型肿瘤坏死因子受体-抗体融合蛋白的纯化方法 |
| US20140128577A1 (en) * | 2011-06-24 | 2014-05-08 | Dr. Reddy's Laboratories Limited | Purification of chimeric protein |
-
2012
- 2012-08-17 KR KR1020120090341A patent/KR101454316B1/ko not_active Expired - Fee Related
- 2012-08-17 EP EP12823797.1A patent/EP2753634B1/en active Active
- 2012-08-17 WO PCT/KR2012/006564 patent/WO2013025079A1/en not_active Ceased
- 2012-08-17 JP JP2014525943A patent/JP6223338B2/ja not_active Expired - Fee Related
- 2012-08-17 US US14/239,216 patent/US9279014B2/en active Active
- 2012-08-17 ES ES12823797.1T patent/ES2651483T3/es active Active
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2016
- 2016-05-26 JP JP2016105574A patent/JP2016168049A/ja active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| US20140316114A1 (en) | 2014-10-23 |
| WO2013025079A1 (en) | 2013-02-21 |
| EP2753634A1 (en) | 2014-07-16 |
| JP2016168049A (ja) | 2016-09-23 |
| EP2753634A4 (en) | 2015-05-20 |
| KR101454316B1 (ko) | 2014-10-27 |
| JP2014521364A (ja) | 2014-08-28 |
| JP6223338B2 (ja) | 2017-11-01 |
| US9279014B2 (en) | 2016-03-08 |
| KR20130020644A (ko) | 2013-02-27 |
| EP2753634B1 (en) | 2017-11-01 |
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