KR101454316B1 - 활성형 ＴＮＦＲ-Ｆｃ 융합 단백질을 제조하는 방법 - Google Patents

활성형 ＴＮＦＲ-Ｆｃ 융합 단백질을 제조하는 방법 Download PDF

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KR101454316B1
KR101454316B1 KR1020120090341A KR20120090341A KR101454316B1 KR 101454316 B1 KR101454316 B1 KR 101454316B1 KR 1020120090341 A KR1020120090341 A KR 1020120090341A KR 20120090341 A KR20120090341 A KR 20120090341A KR 101454316 B1 KR101454316 B1 KR 101454316B1
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South Korea
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tnfr
fusion protein
buffer
protein
column
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KR1020120090341A
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English (en)
Korean (ko)
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KR20130020644A (ko
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원혜순
성병제
안용호
박상경
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한화케미칼 주식회사
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Assigned to 아레스 트레이딩 에스.에이. reassignment 아레스 트레이딩 에스.에이. 권리의 전부이전등록 Assignors: 한화케미칼 주식회사
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/715—Receptors; Cell surface antigens; Cell surface determinants for cytokines; for lymphokines; for interferons
    • C07K14/7151—Receptors; Cell surface antigens; Cell surface determinants for cytokines; for lymphokines; for interferons for tumor necrosis factor [TNF], for lymphotoxin [LT]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/70578—NGF-receptor/TNF-receptor superfamily, e.g. CD27, CD30, CD40, CD95
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K19/00—Hybrid peptides, i.e. peptides covalently bound to nucleic acids, or non-covalently bound protein-protein complexes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/18—Ion-exchange chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/20—Partition-, reverse-phase or hydrophobic interaction chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/30—Non-immunoglobulin-derived peptide or protein having an immunoglobulin constant or Fc region, or a fragment thereof, attached thereto

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Zoology (AREA)
  • Cell Biology (AREA)
  • Analytical Chemistry (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
KR1020120090341A 2011-08-17 2012-08-17 활성형 ＴＮＦＲ-Ｆｃ 융합 단백질을 제조하는 방법 Expired - Fee Related KR101454316B1 (ko)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR1020110081854 2011-08-17
KR20110081854 2011-08-17

Publications (2)

Publication Number Publication Date
KR20130020644A KR20130020644A (ko) 2013-02-27
KR101454316B1 true KR101454316B1 (ko) 2014-10-27

Family

ID=47715279

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KR1020120090341A Expired - Fee Related KR101454316B1 (ko) 2011-08-17 2012-08-17 활성형 ＴＮＦＲ-Ｆｃ 융합 단백질을 제조하는 방법

Country Status (6)

Country Link
US (1) US9279014B2 (https=)
EP (1) EP2753634B1 (https=)
JP (2) JP6223338B2 (https=)
KR (1) KR101454316B1 (https=)
ES (1) ES2651483T3 (https=)
WO (1) WO2013025079A1 (https=)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
HK1200718A1 (en) 2011-10-18 2015-08-14 科荣生生物科学公司 Etanercept formulations stabilized with sodium chloride
JP2015513406A (ja) * 2012-03-12 2015-05-14 ハンファ ケミカル コーポレーション 改変グルタミン合成酵素をコードするポリヌクレオチドを含む発現ベクターおよびこれを用いた標的タンパク質の製造方法
EA031324B1 (ru) * 2012-09-11 2018-12-28 Кохерус Байосайенсис, Инк. Правильно свернутый этанерцепт с высокой чистотой и высоким уровнем выхода
EP3594231A1 (en) 2013-02-13 2020-01-15 Laboratoire Français du Fractionnement et des Biotechnologies Highly galactosylated anti-tnf-alpha antibodies and uses thereof
AU2015273049B2 (en) * 2014-06-13 2019-11-14 Lupin Limited Process for the purification of TNFR:Fc fusion protein
ES2733298T3 (es) 2014-07-18 2019-11-28 Sandoz Ag Cuantificación de TNFR2:Fc plegado erróneamente
EP3241849A4 (en) * 2014-12-31 2018-06-20 LG Chem, Ltd. Method for producing tnfr-fc fusion protein containing target content of impurities
KR20170138426A (ko) * 2015-03-13 2017-12-15 삼성바이오에피스 주식회사 항-tnf-알파 폴리펩티드 조성물 및 그 용도
US11952399B2 (en) * 2018-12-18 2024-04-09 Amgen Inc. Methods for purifying proteins
US12411064B2 (en) 2020-05-08 2025-09-09 Waters Technologies Corporation Methods for heat-assisted enzyme digestion
CN115552215A (zh) 2020-05-08 2022-12-30 沃特世科技公司 用于热辅助酶消化的方法

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
TR200504220T2 (tr) * 1998-12-17 2007-04-24 Biogen Idec Ma Inc. Aktif limfotoksin-beta reseptör imunoglobülin şimeAktif limfotoksin-beta reseptör imunoglobülin şimerik proteinlerinin yüksek düzey ifadesi ve saflaştrik proteinlerinin yüksek düzey ifadesi ve saflaştırılması için bir yöntem.ırılması için bir yöntem.
US7294481B1 (en) 1999-01-05 2007-11-13 Immunex Corporation Method for producing recombinant proteins
PT1946776T (pt) * 2002-02-27 2017-03-17 Immunex Corp Composição de tnfr-fc estabilizada compreendendo arginina
DE602004026343D1 (de) * 2003-10-24 2010-05-12 Amgen Inc Verfahren zur aufreinigung von proteinen in einer durchflussfraktion aus der chromatographie mit hydrophoben wechselwirkungen
US7781395B2 (en) * 2004-01-30 2010-08-24 Immunex Corporation Process for purifying proteins
US20110129468A1 (en) * 2008-02-29 2011-06-02 Biogen Idec Ma Inc. Purified immunoglobulin fusion proteins and methods of their purification
CN102675465A (zh) * 2011-03-09 2012-09-19 上海赛金生物医药有限公司 重组人ⅱ型肿瘤坏死因子受体-抗体融合蛋白的纯化方法
WO2012176158A1 (en) * 2011-06-24 2012-12-27 Dr. Reddy's Laboratories Limited Purification of chimeric protein

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
JOURNAL OF BIOSCIENCE AND BIOENGINEERING (2008) VOL.105, NO.5, pp.454-459 *
JOURNAL OF BIOSCIENCE AND BIOENGINEERING (2008) VOL.105, NO.5, pp.454-459*

Also Published As

Publication number Publication date
EP2753634A4 (en) 2015-05-20
JP2014521364A (ja) 2014-08-28
JP2016168049A (ja) 2016-09-23
EP2753634B1 (en) 2017-11-01
US9279014B2 (en) 2016-03-08
WO2013025079A1 (en) 2013-02-21
US20140316114A1 (en) 2014-10-23
KR20130020644A (ko) 2013-02-27
EP2753634A1 (en) 2014-07-16
ES2651483T3 (es) 2018-01-26
JP6223338B2 (ja) 2017-11-01

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