ES2634428T3 - Amplificación de ácido nucleico con supresión específica de alelo de variantes de secuencia - Google Patents

Amplificación de ácido nucleico con supresión específica de alelo de variantes de secuencia Download PDF

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Publication number
ES2634428T3
ES2634428T3 ES10700205.7T ES10700205T ES2634428T3 ES 2634428 T3 ES2634428 T3 ES 2634428T3 ES 10700205 T ES10700205 T ES 10700205T ES 2634428 T3 ES2634428 T3 ES 2634428T3
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Spain
Prior art keywords
oligonucleotide
target sequence
variant
nucleic acid
amplification
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ES10700205.7T
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English (en)
Spanish (es)
Inventor
Michael Grow
Victoria Brophy
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
F Hoffmann La Roche AG
Roche Diagnostics GmbH
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F Hoffmann La Roche AG
Roche Diagnostics GmbH
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
ES10700205.7T 2009-01-07 2010-01-07 Amplificación de ácido nucleico con supresión específica de alelo de variantes de secuencia Active ES2634428T3 (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US14311309P 2009-01-07 2009-01-07
US143113P 2009-01-07
PCT/EP2010/000030 WO2010079127A1 (en) 2009-01-07 2010-01-07 Nucleic acid amplification with allele-specific suppression of sequence variants

Publications (1)

Publication Number Publication Date
ES2634428T3 true ES2634428T3 (es) 2017-09-27

Family

ID=41682254

Family Applications (1)

Application Number Title Priority Date Filing Date
ES10700205.7T Active ES2634428T3 (es) 2009-01-07 2010-01-07 Amplificación de ácido nucleico con supresión específica de alelo de variantes de secuencia

Country Status (7)

Country Link
US (1) US8206929B2 (https=)
EP (1) EP2385991B1 (https=)
JP (1) JP5785095B2 (https=)
CN (2) CN102272333A (https=)
CA (1) CA2747068C (https=)
ES (1) ES2634428T3 (https=)
WO (1) WO2010079127A1 (https=)

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US10913977B2 (en) 2013-07-24 2021-02-09 Dana-Farber Cancer Institute, Inc. Methods and compositions to enable enrichment of minor DNA alleles by limiting denaturation time in PCR or simply enable enrichment of minor DNA alleles by limiting the denaturation time in PCR
KR101757473B1 (ko) 2013-10-18 2017-07-13 주식회사 씨젠 ｈＣＴＯ를 이용하는 ＰＴＯ 절단 및 연장 분석에 의한 고상에서의 타겟 핵산 서열 검출
CN105283555A (zh) 2013-10-20 2016-01-27 特罗瓦基因公司 核酸序列的合成和富集
WO2015179339A1 (en) * 2014-05-19 2015-11-26 William Marsh Rice University Allele-specific amplification using a composition of overlapping non-allele-specific primer and allele-specific blocker oligonucleotides
JP5813263B1 (ja) * 2015-03-31 2015-11-17 日鉄住金環境株式会社 遺伝子変異の検出方法及びそれに用いる蛍光標識オリゴヌクレオチド
WO2018111835A1 (en) 2016-12-12 2018-06-21 Dana-Farber Cancer Institute, Inc. Compositions and methods for molecular barcoding of dna molecules prior to mutation enrichment and/or mutation detection
US11174511B2 (en) 2017-07-24 2021-11-16 Dana-Farber Cancer Institute, Inc. Methods and compositions for selecting and amplifying DNA targets in a single reaction mixture
CN117858956A (zh) * 2021-08-25 2024-04-09 南京金斯瑞生物科技有限公司 一种用于检测单碱基突变的引物组和基因芯片方法
JP2024531713A (ja) * 2021-09-13 2024-08-29 ネクスジェン キャンサー ディテクション エルエルシー 試料中の標的ポリヌクレオチドを検出するための方法

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Also Published As

Publication number Publication date
CA2747068C (en) 2014-04-08
US8206929B2 (en) 2012-06-26
CN106702000A (zh) 2017-05-24
JP5785095B2 (ja) 2015-09-24
CN102272333A (zh) 2011-12-07
JP2012514451A (ja) 2012-06-28
EP2385991B1 (en) 2017-05-17
EP2385991A1 (en) 2011-11-16
CA2747068A1 (en) 2010-07-15
WO2010079127A1 (en) 2010-07-15
US20100173311A1 (en) 2010-07-08

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