CN102272333A - 利用等位基因特异性抑制序列变体的核酸扩增 - Google Patents

利用等位基因特异性抑制序列变体的核酸扩增 Download PDF

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Publication number
CN102272333A
CN102272333A CN2010800043562A CN201080004356A CN102272333A CN 102272333 A CN102272333 A CN 102272333A CN 2010800043562 A CN2010800043562 A CN 2010800043562A CN 201080004356 A CN201080004356 A CN 201080004356A CN 102272333 A CN102272333 A CN 102272333A
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Prior art keywords
oligonucleotide
target sequence
nucleic acid
amplification
variants
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CN2010800043562A
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Chinese (zh)
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M·格罗
V·布罗菲
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F Hoffmann La Roche AG
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F Hoffmann La Roche AG
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Priority to CN201710109955.4A priority Critical patent/CN106702000A/zh
Publication of CN102272333A publication Critical patent/CN102272333A/zh
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CN2010800043562A 2009-01-07 2010-01-07 利用等位基因特异性抑制序列变体的核酸扩增 Pending CN102272333A (zh)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201710109955.4A CN106702000A (zh) 2009-01-07 2010-01-07 利用等位基因特异性抑制序列变体的核酸扩增

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US14311309P 2009-01-07 2009-01-07
US61/143113 2009-01-07
PCT/EP2010/000030 WO2010079127A1 (en) 2009-01-07 2010-01-07 Nucleic acid amplification with allele-specific suppression of sequence variants

Related Child Applications (1)

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CN201710109955.4A Division CN106702000A (zh) 2009-01-07 2010-01-07 利用等位基因特异性抑制序列变体的核酸扩增

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CN102272333A true CN102272333A (zh) 2011-12-07

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CN2010800043562A Pending CN102272333A (zh) 2009-01-07 2010-01-07 利用等位基因特异性抑制序列变体的核酸扩增
CN201710109955.4A Pending CN106702000A (zh) 2009-01-07 2010-01-07 利用等位基因特异性抑制序列变体的核酸扩增

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Country Link
US (1) US8206929B2 (https=)
EP (1) EP2385991B1 (https=)
JP (1) JP5785095B2 (https=)
CN (2) CN102272333A (https=)
CA (1) CA2747068C (https=)
ES (1) ES2634428T3 (https=)
WO (1) WO2010079127A1 (https=)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2023025218A1 (zh) * 2021-08-25 2023-03-02 南京金斯瑞生物科技有限公司 一种用于检测单碱基突变的引物组和基因芯片方法

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2011224534B2 (en) * 2010-03-08 2014-10-09 Dana-Farber Cancer Institute, Inc. Full COLD-PCR enrichment with reference blocking sequence
EP2691541B1 (en) 2011-03-31 2017-10-18 Dana-Farber Cancer Institute, Inc. Method for enriching in single-stranded mutant sequences from mixture of wild-type and mutant sequences
WO2013010074A1 (en) 2011-07-13 2013-01-17 Primeradx, Inc. Multimodal methods for simultaneous detection and quantification of multiple nucleic acids in a sample
GB201116876D0 (en) 2011-09-30 2011-11-16 Epistem Ltd Mutational analysis of JAK2
AU2014200958B2 (en) 2013-02-25 2016-01-14 Seegene, Inc. Detection of nucleotide variation on target nucleic acid sequence
JP6050555B2 (ja) 2013-07-15 2016-12-21 シージーン アイエヌシー Ｐｔｏの切断及び延長−依存的固定化オリゴヌクレオチドハイブリダイゼーションを用いたターゲット核酸配列の検出
US10913977B2 (en) 2013-07-24 2021-02-09 Dana-Farber Cancer Institute, Inc. Methods and compositions to enable enrichment of minor DNA alleles by limiting denaturation time in PCR or simply enable enrichment of minor DNA alleles by limiting the denaturation time in PCR
KR101757473B1 (ko) 2013-10-18 2017-07-13 주식회사 씨젠 ｈＣＴＯ를 이용하는 ＰＴＯ 절단 및 연장 분석에 의한 고상에서의 타겟 핵산 서열 검출
CN105283555A (zh) 2013-10-20 2016-01-27 特罗瓦基因公司 核酸序列的合成和富集
WO2015179339A1 (en) * 2014-05-19 2015-11-26 William Marsh Rice University Allele-specific amplification using a composition of overlapping non-allele-specific primer and allele-specific blocker oligonucleotides
JP5813263B1 (ja) * 2015-03-31 2015-11-17 日鉄住金環境株式会社 遺伝子変異の検出方法及びそれに用いる蛍光標識オリゴヌクレオチド
WO2018111835A1 (en) 2016-12-12 2018-06-21 Dana-Farber Cancer Institute, Inc. Compositions and methods for molecular barcoding of dna molecules prior to mutation enrichment and/or mutation detection
US11174511B2 (en) 2017-07-24 2021-11-16 Dana-Farber Cancer Institute, Inc. Methods and compositions for selecting and amplifying DNA targets in a single reaction mixture
JP2024531713A (ja) * 2021-09-13 2024-08-29 ネクスジェン キャンサー ディテクション エルエルシー 試料中の標的ポリヌクレオチドを検出するための方法

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20080176226A1 (en) * 2007-01-19 2008-07-24 Chang Gung University Methods and kits for the detection of nucleotide mutations using peptide nucleic acid as both pcr clamp and sensor probe

Family Cites Families (39)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4469863A (en) 1980-11-12 1984-09-04 Ts O Paul O P Nonionic nucleic acid alkyl and aryl phosphonates and processes for manufacture and use thereof
US5235033A (en) 1985-03-15 1993-08-10 Anti-Gene Development Group Alpha-morpholino ribonucleoside derivatives and polymers thereof
US5034506A (en) 1985-03-15 1991-07-23 Anti-Gene Development Group Uncharged morpholino-based polymers having achiral intersubunit linkages
DE3529478A1 (de) 1985-08-16 1987-02-19 Boehringer Mannheim Gmbh 7-desaza-2'desoxyguanosin-nukleotide, verfahren zu deren herstellung und deren verwendung zur nukleinsaeure-sequenzierung
US5374553A (en) 1986-08-22 1994-12-20 Hoffmann-La Roche Inc. DNA encoding a thermostable nucleic acid polymerase enzyme from thermotoga maritima
US5795762A (en) 1986-08-22 1998-08-18 Roche Molecular Systems, Inc. 5' to 3' exonuclease mutations of thermostable DNA polymerases
US5455170A (en) 1986-08-22 1995-10-03 Hoffmann-La Roche Inc. Mutated thermostable nucleic acid polymerase enzyme from Thermus species Z05
US5405774A (en) 1986-08-22 1995-04-11 Hoffmann-La Roche Inc. DNA encoding a mutated thermostable nucleic acid polymerase enzyme from thermus species sps17
US5466591A (en) 1986-08-22 1995-11-14 Hoffmann-La Roche Inc. 5' to 3' exonuclease mutations of thermostable DNA polymerases
US5216141A (en) 1988-06-06 1993-06-01 Benner Steven A Oligonucleotide analogs containing sulfur linkages
US5602240A (en) 1990-07-27 1997-02-11 Ciba Geigy Ag. Backbone modified oligonucleotide analogs
US5386023A (en) 1990-07-27 1995-01-31 Isis Pharmaceuticals Backbone modified oligonucleotide analogs and preparation thereof through reductive coupling
US5210015A (en) 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
CA2092317A1 (en) 1990-09-28 1992-03-29 David H. Gelfand Purified thermostable nucleic acid polymerase enzyme from thermosipho africanus
JPH04234422A (ja) 1990-10-31 1992-08-24 Internatl Business Mach Corp <Ibm> 二重硬化エポキシバックシール処方物
TW393513B (en) 1991-11-26 2000-06-11 Isis Pharmaceuticals Inc Enhanced triple-helix and double-helix formation with oligomers containing modified pyrimidines
US5484908A (en) 1991-11-26 1996-01-16 Gilead Sciences, Inc. Oligonucleotides containing 5-propynyl pyrimidines
AU3222793A (en) 1991-11-26 1993-06-28 Gilead Sciences, Inc. Enhanced triple-helix and double-helix formation with oligomers containing modified pyrimidines
US5644048A (en) 1992-01-10 1997-07-01 Isis Pharmaceuticals, Inc. Process for preparing phosphorothioate oligonucleotides
DE4234086A1 (de) 1992-02-05 1993-08-12 Diagen Inst Molekularbio Verfahren zur bestimmung von in vitro amplifizierten nukleinsaeuresequenzen
US5637684A (en) 1994-02-23 1997-06-10 Isis Pharmaceuticals, Inc. Phosphoramidate and phosphorothioamidate oligomeric compounds
GB9513271D0 (en) 1995-06-29 1995-09-06 Johnson James G Stress free fish harvester
US5773258A (en) 1995-08-25 1998-06-30 Roche Molecular Systems, Inc. Nucleic acid amplification using a reversibly inactivated thermostable enzyme
JP4540754B2 (ja) 1996-06-04 2010-09-08 ユニバーシティ オブ ユタ リサーチ ファウンデーション Ｐｃｒ中のハイブリダイゼーションのモニタリング
EP0866071B1 (en) * 1997-03-20 2004-10-20 F. Hoffmann-La Roche Ag Modified primers
US5849497A (en) * 1997-04-03 1998-12-15 The Research Foundation Of State University Of New York Specific inhibition of the polymerase chain reaction using a non-extendable oligonucleotide blocker
US6228628B1 (en) 1997-07-09 2001-05-08 Roche Molecular Systems Mutant chimeric DNA polymerase
JP3274396B2 (ja) 1997-11-07 2002-04-15 株式会社東芝 パターン測定方法
CN100432237C (zh) * 1999-03-19 2008-11-12 宝生物工程株式会社 用于扩增核酸序列的方法
DE60029314T2 (de) 1999-03-24 2007-07-12 Exiqon A/S Verbesserte Synthese für -2.2.1. I Bicyclo-Nukleoside
WO2000066783A2 (en) 1999-05-04 2000-11-09 Ortho-Clinical Diagnostics, Inc. Rapid and efficient capture of dna from sample without using cell lysing reagent
EA005739B1 (ru) * 2000-08-23 2005-06-30 Такара Био Инк. Способ амплификации нуклеиновых кислот
US6479242B1 (en) * 2000-10-26 2002-11-12 Cleveland State University Method for genotyping of single nucleotide polymorphism
GB0101397D0 (en) * 2001-01-19 2001-03-07 Amersham Pharm Biotech Uk Ltd Suppression of non-specific nucleic acid amplication
EP1253205A1 (en) 2001-04-24 2002-10-30 LION Bioscience AG Method of blocking amplification of selected sequences
US7198897B2 (en) * 2001-12-19 2007-04-03 Brandeis University Late-PCR
ES2371088T3 (es) * 2005-10-05 2011-12-27 F. Hoffmann-La Roche Ag Transferencia de energía no fluorescente.
US8071338B2 (en) * 2007-08-08 2011-12-06 Roche Molecular Systems, Inc. Suppression of amplification using an oligonucleotide and a polymerase significantly lacking 5′-3′ nuclease activity
CA2729649A1 (en) 2008-07-02 2010-01-07 Avexa Limited Thiazopyrimidinones and uses thereof

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20080176226A1 (en) * 2007-01-19 2008-07-24 Chang Gung University Methods and kits for the detection of nucleotide mutations using peptide nucleic acid as both pcr clamp and sensor probe

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
YU D, MUKAI M, LIU Q, STEINMAN CR: "Specific inhibition of PCR by non-extendable oligonucleotides using a 5" to 3" exonuclease-deficient DNA polymerase", 《BIOTECHNIQUES》 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2023025218A1 (zh) * 2021-08-25 2023-03-02 南京金斯瑞生物科技有限公司 一种用于检测单碱基突变的引物组和基因芯片方法

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CA2747068C (en) 2014-04-08
US8206929B2 (en) 2012-06-26
CN106702000A (zh) 2017-05-24
JP5785095B2 (ja) 2015-09-24
JP2012514451A (ja) 2012-06-28
ES2634428T3 (es) 2017-09-27
EP2385991B1 (en) 2017-05-17
EP2385991A1 (en) 2011-11-16
CA2747068A1 (en) 2010-07-15
WO2010079127A1 (en) 2010-07-15
US20100173311A1 (en) 2010-07-08

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