ES2627050T3 - Métodos para realizar hibridaciones con desnaturalización separada de la muestra y la sonda - Google Patents
Métodos para realizar hibridaciones con desnaturalización separada de la muestra y la sonda Download PDFInfo
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- ES2627050T3 ES2627050T3 ES10713513.9T ES10713513T ES2627050T3 ES 2627050 T3 ES2627050 T3 ES 2627050T3 ES 10713513 T ES10713513 T ES 10713513T ES 2627050 T3 ES2627050 T3 ES 2627050T3
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- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
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- C12Q1/6841—In situ hybridisation
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6832—Enhancement of hybridisation reaction
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- C12Q2527/00—Reactions demanding special reaction conditions
- C12Q2527/125—Specific component of sample, medium or buffer
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
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| BRPI0912046B1 (pt) | 2008-05-27 | 2020-11-24 | Agilent Technologies, Inc | Composições de hibridizaqao e seu uso, bem como metodo de hibridizaqao de sequencias de acido nucleico |
| EP2401396B1 (en) * | 2009-02-26 | 2016-11-23 | Dako Denmark A/S | Methods for performing a stringent wash step in hybridization applications |
| US20110117554A1 (en) * | 2009-11-16 | 2011-05-19 | Steven Albert Benner | Formamide-containing mixtures for detecting nucleic acids |
| WO2011067678A2 (en) * | 2009-12-02 | 2011-06-09 | Matthiesen Steen H | Compositions and methods for performing hybridizations with no denaturation |
| WO2013046033A1 (en) * | 2011-09-30 | 2013-04-04 | Dako Denmark A/S | Hybridization compositions and methods using formamide |
| EP3252173A1 (en) | 2011-10-21 | 2017-12-06 | Dako Denmark A/S | Hybridization compositions and methods |
| US10837879B2 (en) | 2011-11-02 | 2020-11-17 | Complete Genomics, Inc. | Treatment for stabilizing nucleic acid arrays |
| RU2498821C1 (ru) * | 2012-04-10 | 2013-11-20 | Федеральное государственное бюджетное образовательное учреждение высшего профессионального образования "Новосибирский национальный исследовательский государственный университет" (Новосибирский государственный университет, НГУ) | Способ стимуляции эндогенной продукции цитокинов и гемопоэтических факторов |
| US20130296173A1 (en) * | 2012-04-23 | 2013-11-07 | Complete Genomics, Inc. | Pre-anchor wash |
| US9783841B2 (en) * | 2012-10-04 | 2017-10-10 | The Board Of Trustees Of The Leland Stanford Junior University | Detection of target nucleic acids in a cellular sample |
| US20140179520A1 (en) * | 2012-12-21 | 2014-06-26 | Brandt Consolidated, Inc. | Humic Acid Composition |
| US9273349B2 (en) * | 2013-03-14 | 2016-03-01 | Affymetrix, Inc. | Detection of nucleic acids |
| WO2015045741A1 (ja) * | 2013-09-26 | 2015-04-02 | 東洋鋼鈑株式会社 | ハイブリダイゼーション用バッファー組成物及びハイブリダイゼーション方法 |
| ES2796701T3 (es) * | 2015-02-04 | 2020-11-30 | Univ Bologna Alma Mater Studiorum | Aditivo para acelerar la hibridación |
| US20180246090A1 (en) * | 2015-02-25 | 2018-08-30 | Sekisui Medical Co., Ltd. | Immunoassay method and assay reagent used in said method |
| KR20170007181A (ko) | 2015-07-10 | 2017-01-18 | 3스캔 인크. | 조직학적 염색제의 공간 다중화 |
| ES2753274T5 (es) * | 2015-08-21 | 2023-05-26 | 42 Life Sciences Gmbh & Co Kg | Composición y procedimiento de hibridación |
| KR20180135476A (ko) | 2016-04-22 | 2018-12-20 | 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 | 세포 구성성분을 매트릭스에 부착시키는 방법 |
| WO2018160907A1 (en) * | 2017-03-03 | 2018-09-07 | Counsyl, Inc. | Extraction of nucleic acids for reduced probe count variability |
| WO2019071262A1 (en) * | 2017-10-06 | 2019-04-11 | The Regents Of The University Of California | RAPID SITU DETECTION OF DNA AND RNA |
| CN107904283A (zh) * | 2017-11-09 | 2018-04-13 | 武汉康录生物技术股份有限公司 | 一种即用型多探针杂交玻片及其制备方法和应用 |
| CN112534061A (zh) * | 2018-07-27 | 2021-03-19 | 豪夫迈·罗氏有限公司 | 用于下一代测序应用的无甲酰胺靶标富集组合物 |
| SG11202101934SA (en) | 2018-07-30 | 2021-03-30 | Readcoor Llc | Methods and systems for sample processing or analysis |
| US10704094B1 (en) | 2018-11-14 | 2020-07-07 | Element Biosciences, Inc. | Multipart reagents having increased avidity for polymerase binding |
| EP3953489A1 (en) | 2019-04-12 | 2022-02-16 | Miltenyi Biotec B.V. & Co. KG | Conjugates having an enzymatically releasable detection moiety and a barcode moiety |
| US12404543B2 (en) | 2019-04-25 | 2025-09-02 | Agilent Technologies, Inc. | Hybridization compositions and methods for making and using compositions |
| US12313627B2 (en) | 2019-05-01 | 2025-05-27 | Element Biosciences, Inc. | Multivalent binding composition for nucleic acid analysis |
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| EP4058596A1 (en) | 2019-11-15 | 2022-09-21 | Miltenyi Biotec B.V. & Co. KG | Color and bardcoded beads for single cell indexing |
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| CN115997126B (zh) | 2020-09-07 | 2025-11-28 | 美天施生物科技有限两合公司 | 具有可酶促释放的检测部分和条形码部分的缀合物 |
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| CN107574230B (zh) | 2022-01-11 |
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| WO2010097655A1 (en) | 2010-09-02 |
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| CN107574230A (zh) | 2018-01-12 |
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| WO2010097707A1 (en) | 2010-09-02 |
| US11795499B2 (en) | 2023-10-24 |
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| US20220154263A1 (en) | 2022-05-19 |
| JP2012518430A (ja) | 2012-08-16 |
| EP2401396A1 (en) | 2012-01-04 |
| CN102369295B (zh) | 2017-11-24 |
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