ES2621547T3 - Sondas de ácido nucleico y métodos para detectar patógenos fúngicos clínicamente importantes - Google Patents
Sondas de ácido nucleico y métodos para detectar patógenos fúngicos clínicamente importantes Download PDFInfo
- Publication number
- ES2621547T3 ES2621547T3 ES00940266.0T ES00940266T ES2621547T3 ES 2621547 T3 ES2621547 T3 ES 2621547T3 ES 00940266 T ES00940266 T ES 00940266T ES 2621547 T3 ES2621547 T3 ES 2621547T3
- Authority
- ES
- Spain
- Prior art keywords
- seq
- probes
- candida
- aspergillus
- species
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 238000000034 method Methods 0.000 title claims abstract description 108
- 244000053095 fungal pathogen Species 0.000 title claims abstract description 25
- 108020004711 Nucleic Acid Probes Proteins 0.000 title description 6
- 239000002853 nucleic acid probe Substances 0.000 title description 6
- 239000000523 sample Substances 0.000 claims abstract description 245
- 108091023242 Internal transcribed spacer Proteins 0.000 claims abstract description 132
- 238000009396 hybridization Methods 0.000 claims abstract description 109
- 241000894007 species Species 0.000 claims abstract description 94
- 238000001514 detection method Methods 0.000 claims abstract description 70
- 230000002538 fungal effect Effects 0.000 claims abstract description 61
- 150000007523 nucleic acids Chemical class 0.000 claims abstract description 61
- 108020004707 nucleic acids Proteins 0.000 claims abstract description 59
- 102000039446 nucleic acids Human genes 0.000 claims abstract description 59
- 241000222122 Candida albicans Species 0.000 claims abstract description 47
- 125000003729 nucleotide group Chemical group 0.000 claims abstract description 34
- 239000002773 nucleotide Substances 0.000 claims abstract description 33
- 241001225321 Aspergillus fumigatus Species 0.000 claims abstract description 29
- 241000221204 Cryptococcus neoformans Species 0.000 claims abstract description 28
- 201000007336 Cryptococcosis Diseases 0.000 claims abstract description 26
- 241000228197 Aspergillus flavus Species 0.000 claims abstract description 24
- 241000235645 Pichia kudriavzevii Species 0.000 claims abstract description 23
- 229940095731 candida albicans Drugs 0.000 claims abstract description 23
- 241000351920 Aspergillus nidulans Species 0.000 claims abstract description 22
- 241000203233 Aspergillus versicolor Species 0.000 claims abstract description 21
- 241000222173 Candida parapsilosis Species 0.000 claims abstract description 21
- 241000222178 Candida tropicalis Species 0.000 claims abstract description 21
- 230000004069 differentiation Effects 0.000 claims abstract description 16
- 229940091771 aspergillus fumigatus Drugs 0.000 claims abstract description 14
- 229940055022 candida parapsilosis Drugs 0.000 claims abstract description 14
- 230000000295 complement effect Effects 0.000 claims abstract description 14
- 230000001717 pathogenic effect Effects 0.000 claims abstract description 14
- 108020005187 Oligonucleotide Probes Proteins 0.000 claims abstract description 13
- 241000222126 [Candida] glabrata Species 0.000 claims abstract description 13
- 208000032343 candida glabrata infection Diseases 0.000 claims abstract description 13
- 238000012217 deletion Methods 0.000 claims abstract description 13
- 230000037430 deletion Effects 0.000 claims abstract description 13
- 239000002751 oligonucleotide probe Substances 0.000 claims abstract description 13
- 239000012141 concentrate Substances 0.000 claims abstract description 5
- 210000004369 blood Anatomy 0.000 claims description 32
- 239000008280 blood Substances 0.000 claims description 32
- 241000228212 Aspergillus Species 0.000 claims description 25
- 108091034117 Oligonucleotide Proteins 0.000 claims description 18
- 239000007787 solid Substances 0.000 claims description 17
- 101100213970 Schizosaccharomyces pombe (strain 972 / ATCC 24843) ypt3 gene Proteins 0.000 claims description 15
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 7
- 101100073099 Schizosaccharomyces pombe (strain 972 / ATCC 24843) its3 gene Proteins 0.000 claims description 5
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 claims description 4
- 239000011324 bead Substances 0.000 claims description 4
- 239000011521 glass Substances 0.000 claims description 4
- 239000012139 lysis buffer Substances 0.000 claims description 4
- 239000008188 pellet Substances 0.000 claims description 4
- 239000011780 sodium chloride Substances 0.000 claims description 4
- 239000006228 supernatant Substances 0.000 claims description 4
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 claims description 3
- 208000006379 syphilis Diseases 0.000 claims 1
- 241000233866 Fungi Species 0.000 abstract description 15
- 230000003321 amplification Effects 0.000 description 35
- 238000003199 nucleic acid amplification method Methods 0.000 description 35
- 241000222120 Candida <Saccharomycetales> Species 0.000 description 33
- 238000007792 addition Methods 0.000 description 29
- 241000233872 Pneumocystis carinii Species 0.000 description 23
- 208000031888 Mycoses Diseases 0.000 description 22
- 238000012360 testing method Methods 0.000 description 22
- 238000006243 chemical reaction Methods 0.000 description 17
- 230000035945 sensitivity Effects 0.000 description 17
- 206010017533 Fungal infection Diseases 0.000 description 16
- 241000144583 Candida dubliniensis Species 0.000 description 14
- 238000003556 assay Methods 0.000 description 14
- 238000003745 diagnosis Methods 0.000 description 14
- 210000004027 cell Anatomy 0.000 description 13
- 208000030507 AIDS Diseases 0.000 description 12
- 206010036790 Productive cough Diseases 0.000 description 12
- 210000003802 sputum Anatomy 0.000 description 12
- 208000024794 sputum Diseases 0.000 description 12
- 241001527609 Cryptococcus Species 0.000 description 11
- 108091093088 Amplicon Proteins 0.000 description 10
- 244000197813 Camelina sativa Species 0.000 description 10
- 244000285963 Kluyveromyces fragilis Species 0.000 description 10
- 238000012408 PCR amplification Methods 0.000 description 9
- 201000003984 candidiasis Diseases 0.000 description 9
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 8
- 206010007134 Candida infections Diseases 0.000 description 8
- 201000010099 disease Diseases 0.000 description 8
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 8
- 238000012986 modification Methods 0.000 description 8
- 230000004048 modification Effects 0.000 description 8
- 230000002441 reversible effect Effects 0.000 description 8
- 239000000243 solution Substances 0.000 description 8
- 238000011282 treatment Methods 0.000 description 8
- 201000002909 Aspergillosis Diseases 0.000 description 7
- 208000036641 Aspergillus infections Diseases 0.000 description 7
- 101150041258 CALB2 gene Proteins 0.000 description 7
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 7
- 239000000427 antigen Substances 0.000 description 7
- 102000036639 antigens Human genes 0.000 description 7
- 108091007433 antigens Proteins 0.000 description 7
- 238000013459 approach Methods 0.000 description 7
- 238000000605 extraction Methods 0.000 description 7
- 208000015181 infectious disease Diseases 0.000 description 7
- 210000004379 membrane Anatomy 0.000 description 7
- 239000012528 membrane Substances 0.000 description 7
- 108700022487 rRNA Genes Proteins 0.000 description 7
- 210000001519 tissue Anatomy 0.000 description 7
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 description 6
- 206010061598 Immunodeficiency Diseases 0.000 description 6
- 101150036851 S100g gene Proteins 0.000 description 6
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 6
- 238000001574 biopsy Methods 0.000 description 6
- 239000000872 buffer Substances 0.000 description 6
- 210000001175 cerebrospinal fluid Anatomy 0.000 description 6
- 101100311260 Caenorhabditis elegans sti-1 gene Proteins 0.000 description 5
- 108020000949 Fungal DNA Proteins 0.000 description 5
- 235000014663 Kluyveromyces fragilis Nutrition 0.000 description 5
- 210000004072 lung Anatomy 0.000 description 5
- 210000002966 serum Anatomy 0.000 description 5
- 238000003786 synthesis reaction Methods 0.000 description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 5
- 101150092532 CALB1 gene Proteins 0.000 description 4
- 108010090804 Streptavidin Proteins 0.000 description 4
- 238000004458 analytical method Methods 0.000 description 4
- 230000015572 biosynthetic process Effects 0.000 description 4
- 238000010790 dilution Methods 0.000 description 4
- 239000012895 dilution Substances 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 4
- 239000003292 glue Substances 0.000 description 4
- 239000003550 marker Substances 0.000 description 4
- 108090000623 proteins and genes Proteins 0.000 description 4
- 210000002700 urine Anatomy 0.000 description 4
- 239000003298 DNA probe Substances 0.000 description 3
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 3
- 208000031886 HIV Infections Diseases 0.000 description 3
- 108091028043 Nucleic acid sequence Proteins 0.000 description 3
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
- 230000000843 anti-fungal effect Effects 0.000 description 3
- 229940121375 antifungal agent Drugs 0.000 description 3
- 229960002685 biotin Drugs 0.000 description 3
- 235000020958 biotin Nutrition 0.000 description 3
- 239000011616 biotin Substances 0.000 description 3
- 230000001684 chronic effect Effects 0.000 description 3
- 238000003759 clinical diagnosis Methods 0.000 description 3
- 230000008878 coupling Effects 0.000 description 3
- 238000010168 coupling process Methods 0.000 description 3
- 238000005859 coupling reaction Methods 0.000 description 3
- 229940079593 drug Drugs 0.000 description 3
- 239000003814 drug Substances 0.000 description 3
- 230000002255 enzymatic effect Effects 0.000 description 3
- 238000011156 evaluation Methods 0.000 description 3
- 229920001519 homopolymer Polymers 0.000 description 3
- 238000002372 labelling Methods 0.000 description 3
- 238000012544 monitoring process Methods 0.000 description 3
- 230000000877 morphologic effect Effects 0.000 description 3
- 210000000056 organ Anatomy 0.000 description 3
- 238000002360 preparation method Methods 0.000 description 3
- 239000000047 product Substances 0.000 description 3
- 210000002345 respiratory system Anatomy 0.000 description 3
- 238000011895 specific detection Methods 0.000 description 3
- 208000024891 symptom Diseases 0.000 description 3
- 108020004463 18S ribosomal RNA Proteins 0.000 description 2
- 108020004565 5.8S Ribosomal RNA Proteins 0.000 description 2
- 102000002260 Alkaline Phosphatase Human genes 0.000 description 2
- 108020004774 Alkaline Phosphatase Proteins 0.000 description 2
- 241000228254 Aspergillus restrictus Species 0.000 description 2
- 208000006081 Cryptococcal meningitis Diseases 0.000 description 2
- 238000007400 DNA extraction Methods 0.000 description 2
- 108010008286 DNA nucleotidylexotransferase Proteins 0.000 description 2
- 102100033215 DNA nucleotidylexotransferase Human genes 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 description 2
- 206010027209 Meningitis cryptococcal Diseases 0.000 description 2
- 241000581002 Murex Species 0.000 description 2
- 206010028980 Neoplasm Diseases 0.000 description 2
- 208000001388 Opportunistic Infections Diseases 0.000 description 2
- 208000005384 Pneumocystis Pneumonia Diseases 0.000 description 2
- 206010035664 Pneumonia Diseases 0.000 description 2
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 2
- 108010006785 Taq Polymerase Proteins 0.000 description 2
- ISAKRJDGNUQOIC-UHFFFAOYSA-N Uracil Chemical compound O=C1C=CNC(=O)N1 ISAKRJDGNUQOIC-UHFFFAOYSA-N 0.000 description 2
- 239000011543 agarose gel Substances 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 238000009640 blood culture Methods 0.000 description 2
- 210000004556 brain Anatomy 0.000 description 2
- 201000011510 cancer Diseases 0.000 description 2
- 238000012512 characterization method Methods 0.000 description 2
- OJYGBLRPYBAHRT-IPQSZEQASA-N chloralose Chemical compound O1[C@H](C(Cl)(Cl)Cl)O[C@@H]2[C@@H](O)[C@@H]([C@H](O)CO)O[C@@H]21 OJYGBLRPYBAHRT-IPQSZEQASA-N 0.000 description 2
- 238000010367 cloning Methods 0.000 description 2
- 230000003247 decreasing effect Effects 0.000 description 2
- 238000004925 denaturation Methods 0.000 description 2
- 230000036425 denaturation Effects 0.000 description 2
- ZMMJGEGLRURXTF-UHFFFAOYSA-N ethidium bromide Chemical compound [Br-].C12=CC(N)=CC=C2C2=CC=C(N)C=C2[N+](CC)=C1C1=CC=CC=C1 ZMMJGEGLRURXTF-UHFFFAOYSA-N 0.000 description 2
- 229960005542 ethidium bromide Drugs 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 239000012530 fluid Substances 0.000 description 2
- 239000012634 fragment Substances 0.000 description 2
- 208000024386 fungal infectious disease Diseases 0.000 description 2
- 210000001035 gastrointestinal tract Anatomy 0.000 description 2
- 238000010562 histological examination Methods 0.000 description 2
- 206010020718 hyperplasia Diseases 0.000 description 2
- 230000002390 hyperplastic effect Effects 0.000 description 2
- 230000003100 immobilizing effect Effects 0.000 description 2
- 238000003018 immunoassay Methods 0.000 description 2
- 239000012678 infectious agent Substances 0.000 description 2
- 239000000138 intercalating agent Substances 0.000 description 2
- 238000002955 isolation Methods 0.000 description 2
- 230000000155 isotopic effect Effects 0.000 description 2
- 230000003902 lesion Effects 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 239000002609 medium Substances 0.000 description 2
- 244000052769 pathogen Species 0.000 description 2
- 239000013612 plasmid Substances 0.000 description 2
- 201000000317 pneumocystosis Diseases 0.000 description 2
- 239000013641 positive control Substances 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 239000011535 reaction buffer Substances 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 230000000241 respiratory effect Effects 0.000 description 2
- 230000004044 response Effects 0.000 description 2
- 238000011896 sensitive detection Methods 0.000 description 2
- 238000012163 sequencing technique Methods 0.000 description 2
- 238000009589 serological test Methods 0.000 description 2
- 239000000758 substrate Substances 0.000 description 2
- 238000002560 therapeutic procedure Methods 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- PLIKAWJENQZMHA-UHFFFAOYSA-N 4-aminophenol Chemical compound NC1=CC=C(O)C=C1 PLIKAWJENQZMHA-UHFFFAOYSA-N 0.000 description 1
- QRXMUCSWCMTJGU-UHFFFAOYSA-N 5-bromo-4-chloro-3-indolyl phosphate Chemical compound C1=C(Br)C(Cl)=C2C(OP(O)(=O)O)=CNC2=C1 QRXMUCSWCMTJGU-UHFFFAOYSA-N 0.000 description 1
- 241000272525 Anas platyrhynchos Species 0.000 description 1
- 101100186130 Arabidopsis thaliana NAC052 gene Proteins 0.000 description 1
- 101100301219 Arabidopsis thaliana RDR6 gene Proteins 0.000 description 1
- 101100529509 Arabidopsis thaliana RECQL4A gene Proteins 0.000 description 1
- 241000131314 Aspergillus candidus Species 0.000 description 1
- 241000228245 Aspergillus niger Species 0.000 description 1
- 241000122824 Aspergillus ochraceus Species 0.000 description 1
- 241001465318 Aspergillus terreus Species 0.000 description 1
- 241000335423 Blastomyces Species 0.000 description 1
- 240000004439 Cistus albidus Species 0.000 description 1
- 241001508813 Clavispora lusitaniae Species 0.000 description 1
- 241000223203 Coccidioides Species 0.000 description 1
- 208000003322 Coinfection Diseases 0.000 description 1
- 241001522864 Cryptococcus gattii VGI Species 0.000 description 1
- 208000014311 Cushing syndrome Diseases 0.000 description 1
- 108020004414 DNA Proteins 0.000 description 1
- 102000053602 DNA Human genes 0.000 description 1
- 238000009007 Diagnostic Kit Methods 0.000 description 1
- SHIBSTMRCDJXLN-UHFFFAOYSA-N Digoxigenin Natural products C1CC(C2C(C3(C)CCC(O)CC3CC2)CC2O)(O)C2(C)C1C1=CC(=O)OC1 SHIBSTMRCDJXLN-UHFFFAOYSA-N 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 241001480035 Epidermophyton Species 0.000 description 1
- 241001136487 Eurotium Species 0.000 description 1
- 241000223218 Fusarium Species 0.000 description 1
- 208000037357 HIV infectious disease Diseases 0.000 description 1
- 206010019233 Headaches Diseases 0.000 description 1
- 208000002250 Hematologic Neoplasms Diseases 0.000 description 1
- 241000711549 Hepacivirus C Species 0.000 description 1
- 241000228402 Histoplasma Species 0.000 description 1
- 241000228404 Histoplasma capsulatum Species 0.000 description 1
- 241000282412 Homo Species 0.000 description 1
- 238000002768 Kirby-Bauer method Methods 0.000 description 1
- 206010025323 Lymphomas Diseases 0.000 description 1
- 241001480037 Microsporum Species 0.000 description 1
- 239000000020 Nitrocellulose Substances 0.000 description 1
- 108091093105 Nuclear DNA Proteins 0.000 description 1
- 101710163270 Nuclease Proteins 0.000 description 1
- 239000004677 Nylon Substances 0.000 description 1
- 206010030216 Oesophagitis Diseases 0.000 description 1
- 208000007027 Oral Candidiasis Diseases 0.000 description 1
- 238000002944 PCR assay Methods 0.000 description 1
- 241000228143 Penicillium Species 0.000 description 1
- 241001507683 Penicillium aurantiogriseum Species 0.000 description 1
- 108091093037 Peptide nucleic acid Proteins 0.000 description 1
- 102000003992 Peroxidases Human genes 0.000 description 1
- 241000233870 Pneumocystis Species 0.000 description 1
- 206010073755 Pneumocystis jirovecii pneumonia Diseases 0.000 description 1
- 206010067565 Pneumonia cryptococcal Diseases 0.000 description 1
- 206010037660 Pyrexia Diseases 0.000 description 1
- 244000088415 Raphanus sativus Species 0.000 description 1
- 235000006140 Raphanus sativus var sativus Nutrition 0.000 description 1
- 108020001027 Ribosomal DNA Proteins 0.000 description 1
- 101100203168 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) SGS1 gene Proteins 0.000 description 1
- 108020004682 Single-Stranded DNA Proteins 0.000 description 1
- 241001149962 Sporothrix Species 0.000 description 1
- 206010042938 Systemic candida Diseases 0.000 description 1
- RYYWUUFWQRZTIU-UHFFFAOYSA-N Thiophosphoric acid Chemical class OP(O)(S)=O RYYWUUFWQRZTIU-UHFFFAOYSA-N 0.000 description 1
- 241000223238 Trichophyton Species 0.000 description 1
- 241000223231 Trichosporon beigelii Species 0.000 description 1
- 102000006943 Uracil-DNA Glycosidase Human genes 0.000 description 1
- 108010072685 Uracil-DNA Glycosidase Proteins 0.000 description 1
- QCWXUUIWCKQGHC-UHFFFAOYSA-N Zirconium Chemical compound [Zr] QCWXUUIWCKQGHC-UHFFFAOYSA-N 0.000 description 1
- 230000001154 acute effect Effects 0.000 description 1
- 230000006978 adaptation Effects 0.000 description 1
- 125000001931 aliphatic group Chemical group 0.000 description 1
- -1 alkyl phosphorothiates Chemical class 0.000 description 1
- 239000012491 analyte Substances 0.000 description 1
- 230000005875 antibody response Effects 0.000 description 1
- 238000011888 autopsy Methods 0.000 description 1
- 238000012742 biochemical analysis Methods 0.000 description 1
- 210000001124 body fluid Anatomy 0.000 description 1
- 239000010839 body fluid Substances 0.000 description 1
- 210000000988 bone and bone Anatomy 0.000 description 1
- 238000010322 bone marrow transplantation Methods 0.000 description 1
- 230000001364 causal effect Effects 0.000 description 1
- 230000034303 cell budding Effects 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 210000003756 cervix mucus Anatomy 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000002512 chemotherapy Methods 0.000 description 1
- 230000002860 competitive effect Effects 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000012790 confirmation Methods 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 238000010411 cooking Methods 0.000 description 1
- 239000003246 corticosteroid Substances 0.000 description 1
- 229960001334 corticosteroids Drugs 0.000 description 1
- 230000001351 cycling effect Effects 0.000 description 1
- 230000009089 cytolysis Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- QONQRTHLHBTMGP-UHFFFAOYSA-N digitoxigenin Natural products CC12CCC(C3(CCC(O)CC3CC3)C)C3C11OC1CC2C1=CC(=O)OC1 QONQRTHLHBTMGP-UHFFFAOYSA-N 0.000 description 1
- SHIBSTMRCDJXLN-KCZCNTNESA-N digoxigenin Chemical compound C1([C@@H]2[C@@]3([C@@](CC2)(O)[C@H]2[C@@H]([C@@]4(C)CC[C@H](O)C[C@H]4CC2)C[C@H]3O)C)=CC(=O)OC1 SHIBSTMRCDJXLN-KCZCNTNESA-N 0.000 description 1
- 238000006073 displacement reaction Methods 0.000 description 1
- 208000019164 disseminated candidiasis Diseases 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 238000013399 early diagnosis Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 206010014665 endocarditis Diseases 0.000 description 1
- 210000003743 erythrocyte Anatomy 0.000 description 1
- 208000006881 esophagitis Diseases 0.000 description 1
- 239000004744 fabric Substances 0.000 description 1
- 210000003608 fece Anatomy 0.000 description 1
- GNBHRKFJIUUOQI-UHFFFAOYSA-N fluorescein Chemical compound O1C(=O)C2=CC=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 GNBHRKFJIUUOQI-UHFFFAOYSA-N 0.000 description 1
- 238000005194 fractionation Methods 0.000 description 1
- 239000000499 gel Substances 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 231100000869 headache Toxicity 0.000 description 1
- 208000013210 hematogenous Diseases 0.000 description 1
- 230000000652 homosexual effect Effects 0.000 description 1
- 208000033519 human immunodeficiency virus infectious disease Diseases 0.000 description 1
- 244000052637 human pathogen Species 0.000 description 1
- 230000007124 immune defense Effects 0.000 description 1
- 230000028993 immune response Effects 0.000 description 1
- 210000000987 immune system Anatomy 0.000 description 1
- 238000003119 immunoblot Methods 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 238000010348 incorporation Methods 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 239000003112 inhibitor Substances 0.000 description 1
- 201000009085 invasive aspergillosis Diseases 0.000 description 1
- 238000003771 laboratory diagnosis Methods 0.000 description 1
- 238000012092 latex agglutination test Methods 0.000 description 1
- 208000032839 leukemia Diseases 0.000 description 1
- 238000007834 ligase chain reaction Methods 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 206010025135 lupus erythematosus Diseases 0.000 description 1
- 108010026228 mRNA guanylyltransferase Proteins 0.000 description 1
- 229910001629 magnesium chloride Inorganic materials 0.000 description 1
- 206010025482 malaise Diseases 0.000 description 1
- 230000013011 mating Effects 0.000 description 1
- 210000002418 meninge Anatomy 0.000 description 1
- 239000002207 metabolite Substances 0.000 description 1
- 239000004005 microsphere Substances 0.000 description 1
- 238000005497 microtitration Methods 0.000 description 1
- 239000003068 molecular probe Substances 0.000 description 1
- 210000002200 mouth mucosa Anatomy 0.000 description 1
- 239000013642 negative control Substances 0.000 description 1
- 229920001220 nitrocellulos Polymers 0.000 description 1
- 229920001778 nylon Polymers 0.000 description 1
- 229940046166 oligodeoxynucleotide Drugs 0.000 description 1
- 238000000424 optical density measurement Methods 0.000 description 1
- 230000001575 pathological effect Effects 0.000 description 1
- 230000037361 pathway Effects 0.000 description 1
- 108040007629 peroxidase activity proteins Proteins 0.000 description 1
- 238000003752 polymerase chain reaction Methods 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 239000002243 precursor Substances 0.000 description 1
- 201000009395 primary hyperaldosteronism Diseases 0.000 description 1
- 230000037452 priming Effects 0.000 description 1
- 235000018102 proteins Nutrition 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 230000002285 radioactive effect Effects 0.000 description 1
- 238000003127 radioimmunoassay Methods 0.000 description 1
- 230000000306 recurrent effect Effects 0.000 description 1
- 230000002829 reductive effect Effects 0.000 description 1
- 238000007894 restriction fragment length polymorphism technique Methods 0.000 description 1
- 238000012552 review Methods 0.000 description 1
- 108020004418 ribosomal RNA Proteins 0.000 description 1
- 210000003296 saliva Anatomy 0.000 description 1
- 238000005464 sample preparation method Methods 0.000 description 1
- 201000000306 sarcoidosis Diseases 0.000 description 1
- 239000013049 sediment Substances 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 230000000405 serological effect Effects 0.000 description 1
- 239000000377 silicon dioxide Substances 0.000 description 1
- 210000003491 skin Anatomy 0.000 description 1
- 239000001509 sodium citrate Substances 0.000 description 1
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 description 1
- 206010041823 squamous cell carcinoma Diseases 0.000 description 1
- 238000010186 staining Methods 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 239000011550 stock solution Substances 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 230000009885 systemic effect Effects 0.000 description 1
- 239000013077 target material Substances 0.000 description 1
- 230000001225 therapeutic effect Effects 0.000 description 1
- 238000013518 transcription Methods 0.000 description 1
- 230000035897 transcription Effects 0.000 description 1
- 230000001052 transient effect Effects 0.000 description 1
- 238000011269 treatment regimen Methods 0.000 description 1
- 239000001226 triphosphate Substances 0.000 description 1
- 235000011178 triphosphate Nutrition 0.000 description 1
- UNXRWKVEANCORM-UHFFFAOYSA-N triphosphoric acid Chemical compound OP(O)(=O)OP(O)(=O)OP(O)(O)=O UNXRWKVEANCORM-UHFFFAOYSA-N 0.000 description 1
- 229940035893 uracil Drugs 0.000 description 1
- 210000002229 urogenital system Anatomy 0.000 description 1
- 210000001215 vagina Anatomy 0.000 description 1
- 210000001835 viscera Anatomy 0.000 description 1
- 210000005253 yeast cell Anatomy 0.000 description 1
- 229910052726 zirconium Inorganic materials 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Immunology (AREA)
- Mycology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Botany (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP99870109 | 1999-05-28 | ||
| EP99870109 | 1999-05-28 | ||
| US13862199P | 1999-06-11 | 1999-06-11 | |
| US138621P | 1999-06-11 | ||
| PCT/EP2000/004714 WO2000073499A2 (en) | 1999-05-28 | 2000-05-24 | Nucleic acid probes and methods for detecting clinically important fungal pathogens |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ES2621547T3 true ES2621547T3 (es) | 2017-07-04 |
Family
ID=46506639
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES00940266.0T Expired - Lifetime ES2621547T3 (es) | 1999-05-28 | 2000-05-24 | Sondas de ácido nucleico y métodos para detectar patógenos fúngicos clínicamente importantes |
Country Status (7)
| Country | Link |
|---|---|
| US (2) | US6858387B1 (enExample) |
| EP (1) | EP1185702B1 (enExample) |
| JP (2) | JP4963765B2 (enExample) |
| AU (1) | AU782305B2 (enExample) |
| CA (1) | CA2374878C (enExample) |
| ES (1) | ES2621547T3 (enExample) |
| WO (1) | WO2000073499A2 (enExample) |
Families Citing this family (45)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP4963765B2 (ja) * | 1999-05-28 | 2012-06-27 | イノジェネティックス・ナムローゼ・フェンノートシャップ | 臨床的に重要な真菌病原体を検出するための核酸プローブおよび方法 |
| WO2001083821A2 (en) * | 2000-05-01 | 2001-11-08 | Gen-Probe Incorporated | Polynucleotide probes for detection and quantitation of candida species |
| WO2002079512A2 (en) * | 2001-03-29 | 2002-10-10 | University Of Nevada - Las Vegas | Method for detection and quantification of the fungus aspergillus fumigatus using quantitative pcr |
| WO2003080866A1 (en) * | 2002-03-26 | 2003-10-02 | Council Of Scientific And Industrial Research | Novel primers for identifying aflatoxinogenic aspergilli and an improved use thereof |
| JP4377810B2 (ja) | 2002-05-17 | 2009-12-02 | アメリカ合衆国 | アスペルギルス種の分子的同定方法 |
| JP2004290171A (ja) * | 2003-02-07 | 2004-10-21 | Akira Hiraishi | 微生物の分子生物学的同定技術 |
| EP1690938A1 (de) * | 2005-02-11 | 2006-08-16 | Qiagen GmbH | Verfahren zur Isolierung von Nukleinsäuren, wobei die Nukleinsäuren bei erhöhter Temperatur an einer Matrix immobilisiert werden |
| US20100311041A1 (en) | 2005-06-14 | 2010-12-09 | Statens Serum Institut | Pcr diagnostics of dermatophytes and other pathogenic fungi |
| US20070050311A1 (en) * | 2005-08-30 | 2007-03-01 | Caterpillar Inc. | Assembly should cost application |
| EP1945810B1 (en) * | 2005-09-20 | 2015-07-01 | AdvanDx, Inc. | Reagents, methods and kits for classification of fungi and direction of anti-fungal therapy |
| JP2007159411A (ja) | 2005-12-09 | 2007-06-28 | Canon Inc | プローブセット、プローブ固定担体及び遺伝子検査方法 |
| WO2007101664A2 (de) * | 2006-03-08 | 2007-09-13 | Fungus Bioscience Gmbh | Verbesserter aspergillosetest in klinischen proben |
| RU2348695C2 (ru) | 2006-05-23 | 2009-03-10 | Закрытое акционерное общество "Молекулярно-медицинские технологии" | Дифференцирующий и специфический олигонуклеотиды для идентификации последовательностей днк инфекционных агентов в биологических материалах, способ видовой идентификации инфекционных агентов, биочип и набор для осуществления этого способа |
| JP5015518B2 (ja) * | 2006-08-04 | 2012-08-29 | 東洋鋼鈑株式会社 | ハウスダストアレルギーの原因となるダニ・カビ類の検出・識別用マイクロアレイ |
| PT103729A (pt) * | 2007-05-04 | 2008-11-04 | Stab Vida Investigacao E Servi | Estojo e processo para a detecção e identificação de leveduras de interesse clínico, através de uma amplificação isotérmica de adn seguida de hibridação com oligonucleótidos específicos, e respectivas utilizações. |
| JP5196847B2 (ja) * | 2007-05-14 | 2013-05-15 | キヤノン株式会社 | プローブセット、プローブ担体及び検査方法 |
| JP5196850B2 (ja) * | 2007-05-14 | 2013-05-15 | キヤノン株式会社 | プローブセット、プローブ担体及び検査方法 |
| JP5196843B2 (ja) | 2007-05-14 | 2013-05-15 | キヤノン株式会社 | プローブセット、プローブ担体及び検査方法 |
| JP5317430B2 (ja) * | 2007-05-14 | 2013-10-16 | キヤノン株式会社 | プローブセット、プローブ担体、及び真菌の判別同定方法 |
| JP5196866B2 (ja) * | 2007-05-14 | 2013-05-15 | キヤノン株式会社 | プローブセット、プローブ担体及び検査方法 |
| JP5196845B2 (ja) * | 2007-05-14 | 2013-05-15 | キヤノン株式会社 | プローブセット、プローブ担体及び検査方法 |
| JP5196846B2 (ja) * | 2007-05-14 | 2013-05-15 | キヤノン株式会社 | プローブセット、プローブ担体、検査方法及びｄｎａ検出用キット |
| JP5196856B2 (ja) * | 2007-05-14 | 2013-05-15 | キヤノン株式会社 | プローブセット、プローブ担体及び検査方法 |
| JP5196861B2 (ja) | 2007-05-14 | 2013-05-15 | キヤノン株式会社 | プローブセット、プローブ担体及び検査方法 |
| JP5196853B2 (ja) * | 2007-05-14 | 2013-05-15 | キヤノン株式会社 | プローブセット、プローブ担体及び検査方法 |
| JP5196859B2 (ja) * | 2007-05-14 | 2013-05-15 | キヤノン株式会社 | プローブセット、プローブ担体、検査方法及びｄｎａ検出用キット |
| JP5196865B2 (ja) * | 2007-05-14 | 2013-05-15 | キヤノン株式会社 | プローブセット、プローブ担体及び検査方法 |
| JP5196860B2 (ja) * | 2007-05-14 | 2013-05-15 | キヤノン株式会社 | プローブセット、プローブ担体及び検査方法 |
| JP5196858B2 (ja) * | 2007-05-14 | 2013-05-15 | キヤノン株式会社 | プローブセット、プローブ担体、検査方法及びｄｎａ検出用キット |
| JP5196854B2 (ja) * | 2007-05-14 | 2013-05-15 | キヤノン株式会社 | プローブセット、プローブ担体及び検査方法 |
| US7829735B2 (en) * | 2007-10-26 | 2010-11-09 | Northwestern University | Universal phosphoramidite for preparation of modified biomolecules and surfaces |
| CA2715991A1 (en) | 2007-12-26 | 2009-07-09 | Gen-Probe Incorporated | Amplification oligomers and methods to detect candida albicans 26s rrna or encoding dna |
| CA2720292A1 (en) * | 2008-04-01 | 2009-10-08 | Metametrix Clinical Laboratory | Process and method for monitoring gastrointestinal microbiota |
| ES2335179B1 (es) * | 2008-09-19 | 2011-09-14 | Instituto De Salud Carlos Iii | Metodo para la deteccion simultanea de histoplasma capsulatum y paracoccidioides brasiliensis. |
| MX352246B (es) | 2009-03-18 | 2017-10-25 | Instituto Potosino De Investig Cientifica Y Tecnologica A C | Metodo in vitro para la deteccion de candida glabrata, kit de diagnostico y usos de los mismos. |
| PL2536850T3 (pl) | 2010-02-15 | 2017-09-29 | Council Of Scientific & Industrial Research | Sposób wykrywania patogenów grzybiczych |
| US10214780B2 (en) | 2012-03-09 | 2019-02-26 | City University Of Hong Kong | Method and means for identification of animal species |
| EP2636753B1 (en) * | 2012-03-09 | 2018-07-25 | City University of Hong Kong | Method and means for identification of animal species |
| US10253353B2 (en) * | 2013-12-06 | 2019-04-09 | The Broad Institute, Inc. | Enhanced methods of ribonucleic acid hybridization |
| CN105420372A (zh) * | 2015-12-17 | 2016-03-23 | 安徽出入境检验检疫局检验检疫技术中心 | 检测梨黑斑病菌的pcr引物以及使用该引物的检测方法 |
| CN107130015B (zh) * | 2016-02-29 | 2023-07-11 | 上海翔琼生物技术有限公司 | 一种检测烟曲霉菌的荧光定量pcr试剂盒 |
| CN106497796A (zh) * | 2016-07-20 | 2017-03-15 | 辽宁省微生物科学研究院 | 一种食用菌菌种的单克隆高效选育方法 |
| CN112626263B (zh) * | 2021-01-20 | 2022-08-05 | 中国人民解放军总医院 | 酶切探针恒温检测呼吸道感染真菌病原体的试剂盒 |
| CN112941231A (zh) * | 2021-04-26 | 2021-06-11 | 南方海洋科学与工程广东省实验室(湛江) | 一种用于白色念珠菌检测的探针、方法及应用 |
| EP4577673A1 (en) * | 2022-08-26 | 2025-07-02 | Denka Company Limited | Methods for the detection and identification of fungal species |
Family Cites Families (21)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US546027A (en) * | 1895-09-10 | Edgar charles parker | ||
| US4617102A (en) * | 1985-03-21 | 1986-10-14 | Lifecodes Corp. | Process and apparatus for purifying and concentrating DNA from crude mixtures containing DNA |
| US5541308A (en) * | 1986-11-24 | 1996-07-30 | Gen-Probe Incorporated | Nucleic acid probes for detection and/or quantitation of non-viral organisms |
| AU7429591A (en) * | 1990-04-18 | 1991-10-24 | Gene-Trak Systems | Nucleic acid probes for the detection of giardia lamblia |
| DE69332202T2 (de) | 1992-05-18 | 2003-05-28 | Richard David Cannon | Methoden zur diagnose von pilzinfektionen |
| US5426027A (en) | 1993-05-20 | 1995-06-20 | The Government Of The United States Of America As Represented By The Secretary | Nucleic acid probes and methods for detecting Candida DNA cells in blood |
| US6017699A (en) * | 1993-09-15 | 2000-01-25 | The University Of Pittsburgh | PCR identification and quantification of important Candida species |
| US5585238A (en) * | 1994-04-25 | 1996-12-17 | Ciba-Geigy Corporation | Detection of fungal pathogens using the polymerase chain reaction |
| US5763169A (en) * | 1995-01-13 | 1998-06-09 | Chiron Diagnostics Corporation | Nucleic acid probes for the detection and identification of fungi |
| US5693501A (en) | 1995-03-08 | 1997-12-02 | Indiana University Advanced Research & Technology Institute | Compounds and methods to determine presence of Histoplasma capsulatum |
| EP0846186B1 (de) | 1995-08-17 | 2008-02-20 | Myconostica Limited | Extraktion, amplifikation und sequentielle hybridisierung von pilzzellen-dna sowie verfahren zum nachweisen von pilzzellen in klinischem material |
| FR2749011B1 (fr) | 1996-03-22 | 1998-08-28 | Univ Claude Bernard Lyon | Sonde, sequence nucleiques et procede notamment, de detection et d'identification de levures, de meme que les outils genetiques correspondants |
| US5874221A (en) | 1996-08-28 | 1999-02-23 | The United States Of America As Represented By The Secretary Of Agriculture | Species specific method for the PCR detection of phythophthora |
| US6235890B1 (en) * | 1996-09-16 | 2001-05-22 | The United States Of America As Represented By The Department Of Health And Human Services | Methods and compositions for the detection of Candida spp. |
| US5814453A (en) | 1996-10-15 | 1998-09-29 | Novartis Finance Corporation | Detection of fungal pathogens using the polymerase chain reaction |
| US5800997A (en) | 1996-11-01 | 1998-09-01 | Novartis Finance Corporation | Detection of maize fungal pathogens using the polymerase chain reaction |
| ATE320506T1 (de) * | 1997-05-02 | 2006-04-15 | Us Gov Health & Human Serv | Nukleinsäuren zur erkennung von aspergillus spezien |
| US6242178B1 (en) * | 1997-07-30 | 2001-06-05 | Centers For Disease Control And Prevention | Nucleic acid probes for detecting Candida species |
| US5827695A (en) | 1997-08-04 | 1998-10-27 | Novartis Finance Corporation | Detection of wheat fungal pathogens using the polymerase chain reaction |
| US6080543A (en) | 1997-12-08 | 2000-06-27 | E. & J. Gallo Winery | Detection of fungal pathogens |
| JP4963765B2 (ja) * | 1999-05-28 | 2012-06-27 | イノジェネティックス・ナムローゼ・フェンノートシャップ | 臨床的に重要な真菌病原体を検出するための核酸プローブおよび方法 |
-
2000
- 2000-05-24 JP JP2001500809A patent/JP4963765B2/ja not_active Expired - Lifetime
- 2000-05-24 WO PCT/EP2000/004714 patent/WO2000073499A2/en not_active Ceased
- 2000-05-24 AU AU55255/00A patent/AU782305B2/en not_active Ceased
- 2000-05-24 CA CA2374878A patent/CA2374878C/en not_active Expired - Lifetime
- 2000-05-24 EP EP00940266.0A patent/EP1185702B1/en not_active Expired - Lifetime
- 2000-05-24 ES ES00940266.0T patent/ES2621547T3/es not_active Expired - Lifetime
- 2000-09-15 US US09/662,462 patent/US6858387B1/en not_active Expired - Lifetime
-
2004
- 2004-10-19 US US10/967,254 patent/US7741461B2/en not_active Expired - Fee Related
-
2012
- 2012-01-26 JP JP2012014151A patent/JP2012120535A/ja active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| EP1185702B1 (en) | 2017-02-08 |
| US20050164243A1 (en) | 2005-07-28 |
| CA2374878C (en) | 2014-10-14 |
| JP4963765B2 (ja) | 2012-06-27 |
| JP2012120535A (ja) | 2012-06-28 |
| WO2000073499A2 (en) | 2000-12-07 |
| WO2000073499A3 (en) | 2001-08-09 |
| US6858387B1 (en) | 2005-02-22 |
| EP1185702A2 (en) | 2002-03-13 |
| JP2003501049A (ja) | 2003-01-14 |
| AU5525500A (en) | 2000-12-18 |
| AU782305B2 (en) | 2005-07-21 |
| CA2374878A1 (en) | 2000-12-07 |
| US7741461B2 (en) | 2010-06-22 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| ES2621547T3 (es) | Sondas de ácido nucleico y métodos para detectar patógenos fúngicos clínicamente importantes | |
| US12378613B2 (en) | Assays for fungal infection | |
| US7052836B2 (en) | Nucleic acids for detecting fusarium species | |
| US6469156B1 (en) | Rapid and sensitive method for detecting histoplasma capsulatum | |
| JP2002504817A (ja) | 真菌の検出および同定のための核酸プローブ | |
| JP4886784B2 (ja) | カンジダ種の検出のための組成物および方法 | |
| EP2529034B1 (en) | Methods for the detection of fungus | |
| CN113151557A (zh) | 用于检测荚膜组织胞浆菌的lamp引物组、试剂盒及方法 | |
| Meletiadis et al. | Evaluation of a polymerase chain reaction reverse hybridization line probe assay for the detection and identification of medically important fungi in bronchoalveolar lavage fluids | |
| Arancia et al. | Use of 65 kDa mannoprotein gene primers in real time PCR identification of Candida albicans in biological samples | |
| Arancia et al. | Construction and use of PCR primers from a 65 kDa mannoprotein gene for identification of C. albicans | |
| JP5621992B2 (ja) | カンジダ種を検出および同定するための方法 | |
| AU3658899A (en) | Rapid and sensitive method for detecting (histoplasma capsulatum) | |
| Rodriguez-Villalobos et al. | Cross reaction between a pan-Candida genus probe and Fusarium spp. in a fatal case of Fusarium oxysporum pneumonia | |
| Rossau et al. | Development of a PCR-Based Line Probe |