CA2374878C - Nucleic acid probes and methods for detecting clinically important fungal pathogens - Google Patents
Nucleic acid probes and methods for detecting clinically important fungal pathogens Download PDFInfo
- Publication number
- CA2374878C CA2374878C CA2374878A CA2374878A CA2374878C CA 2374878 C CA2374878 C CA 2374878C CA 2374878 A CA2374878 A CA 2374878A CA 2374878 A CA2374878 A CA 2374878A CA 2374878 C CA2374878 C CA 2374878C
- Authority
- CA
- Canada
- Prior art keywords
- sample
- seq
- candida
- probes
- hybridization complexes
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 238000000034 method Methods 0.000 title claims abstract 46
- 244000053095 fungal pathogen Species 0.000 title claims 9
- 108020004711 Nucleic Acid Probes Proteins 0.000 title 1
- 239000002853 nucleic acid probe Substances 0.000 title 1
- 239000000523 sample Substances 0.000 claims abstract 73
- 241000894007 species Species 0.000 claims abstract 11
- 108091023242 Internal transcribed spacer Proteins 0.000 claims abstract 7
- 241000228197 Aspergillus flavus Species 0.000 claims abstract 6
- 241001225321 Aspergillus fumigatus Species 0.000 claims abstract 6
- 241000351920 Aspergillus nidulans Species 0.000 claims abstract 6
- 241000203233 Aspergillus versicolor Species 0.000 claims abstract 6
- 241000222122 Candida albicans Species 0.000 claims abstract 6
- 241000144583 Candida dubliniensis Species 0.000 claims abstract 6
- 241000222173 Candida parapsilosis Species 0.000 claims abstract 6
- 241000222178 Candida tropicalis Species 0.000 claims abstract 6
- 241000235645 Pichia kudriavzevii Species 0.000 claims abstract 6
- 241000222126 [Candida] glabrata Species 0.000 claims abstract 6
- 230000002538 fungal effect Effects 0.000 claims abstract 6
- 201000007336 Cryptococcosis Diseases 0.000 claims abstract 4
- 241000221204 Cryptococcus neoformans Species 0.000 claims abstract 4
- 235000014663 Kluyveromyces fragilis Nutrition 0.000 claims abstract 4
- 241000233872 Pneumocystis carinii Species 0.000 claims abstract 4
- 229940091771 aspergillus fumigatus Drugs 0.000 claims abstract 4
- 229940095731 candida albicans Drugs 0.000 claims abstract 4
- 208000032343 candida glabrata infection Diseases 0.000 claims abstract 4
- 229940055022 candida parapsilosis Drugs 0.000 claims abstract 4
- 238000001514 detection method Methods 0.000 claims abstract 4
- 238000009396 hybridization Methods 0.000 claims 31
- 108020004707 nucleic acids Proteins 0.000 claims 18
- 150000007523 nucleic acids Chemical class 0.000 claims 18
- 102000039446 nucleic acids Human genes 0.000 claims 18
- 239000002773 nucleotide Substances 0.000 claims 9
- 125000003729 nucleotide group Chemical group 0.000 claims 9
- 238000007792 addition Methods 0.000 claims 8
- 238000012217 deletion Methods 0.000 claims 8
- 230000037430 deletion Effects 0.000 claims 8
- 244000285963 Kluyveromyces fragilis Species 0.000 claims 5
- 108020005187 Oligonucleotide Probes Proteins 0.000 claims 3
- 230000000295 complement effect Effects 0.000 claims 3
- 239000002751 oligonucleotide probe Substances 0.000 claims 3
- 239000007787 solid Substances 0.000 claims 3
- 108091034117 Oligonucleotide Proteins 0.000 claims 2
- 101100213970 Schizosaccharomyces pombe (strain 972 / ATCC 24843) ypt3 gene Proteins 0.000 claims 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims 2
- 239000008280 blood Substances 0.000 claims 2
- 210000004369 blood Anatomy 0.000 claims 2
- 244000052769 pathogen Species 0.000 claims 2
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 claims 1
- 241000228212 Aspergillus Species 0.000 claims 1
- 241000222120 Candida <Saccharomycetales> Species 0.000 claims 1
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 claims 1
- 101100073099 Schizosaccharomyces pombe (strain 972 / ATCC 24843) its3 gene Proteins 0.000 claims 1
- 238000003556 assay Methods 0.000 claims 1
- 239000011324 bead Substances 0.000 claims 1
- 238000005119 centrifugation Methods 0.000 claims 1
- 230000004069 differentiation Effects 0.000 claims 1
- 239000011521 glass Substances 0.000 claims 1
- 238000011534 incubation Methods 0.000 claims 1
- 239000012139 lysis buffer Substances 0.000 claims 1
- 239000008188 pellet Substances 0.000 claims 1
- 239000011780 sodium chloride Substances 0.000 claims 1
- 239000006228 supernatant Substances 0.000 claims 1
- 238000003260 vortexing Methods 0.000 claims 1
- 241000233866 Fungi Species 0.000 abstract 1
- 241000235650 Kluyveromyces marxianus Species 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Immunology (AREA)
- Mycology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Botany (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP99870109.8 | 1999-05-28 | ||
| EP99870109 | 1999-05-28 | ||
| US13862199P | 1999-06-11 | 1999-06-11 | |
| US60/138,621 | 1999-06-11 | ||
| PCT/EP2000/004714 WO2000073499A2 (en) | 1999-05-28 | 2000-05-24 | Nucleic acid probes and methods for detecting clinically important fungal pathogens |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2374878A1 CA2374878A1 (en) | 2000-12-07 |
| CA2374878C true CA2374878C (en) | 2014-10-14 |
Family
ID=46506639
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2374878A Expired - Lifetime CA2374878C (en) | 1999-05-28 | 2000-05-24 | Nucleic acid probes and methods for detecting clinically important fungal pathogens |
Country Status (7)
| Country | Link |
|---|---|
| US (2) | US6858387B1 (enExample) |
| EP (1) | EP1185702B1 (enExample) |
| JP (2) | JP4963765B2 (enExample) |
| AU (1) | AU782305B2 (enExample) |
| CA (1) | CA2374878C (enExample) |
| ES (1) | ES2621547T3 (enExample) |
| WO (1) | WO2000073499A2 (enExample) |
Families Citing this family (45)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP4963765B2 (ja) * | 1999-05-28 | 2012-06-27 | イノジェネティックス・ナムローゼ・フェンノートシャップ | 臨床的に重要な真菌病原体を検出するための核酸プローブおよび方法 |
| WO2001083821A2 (en) * | 2000-05-01 | 2001-11-08 | Gen-Probe Incorporated | Polynucleotide probes for detection and quantitation of candida species |
| WO2002079512A2 (en) * | 2001-03-29 | 2002-10-10 | University Of Nevada - Las Vegas | Method for detection and quantification of the fungus aspergillus fumigatus using quantitative pcr |
| WO2003080866A1 (en) * | 2002-03-26 | 2003-10-02 | Council Of Scientific And Industrial Research | Novel primers for identifying aflatoxinogenic aspergilli and an improved use thereof |
| JP4377810B2 (ja) | 2002-05-17 | 2009-12-02 | アメリカ合衆国 | アスペルギルス種の分子的同定方法 |
| JP2004290171A (ja) * | 2003-02-07 | 2004-10-21 | Akira Hiraishi | 微生物の分子生物学的同定技術 |
| EP1690938A1 (de) * | 2005-02-11 | 2006-08-16 | Qiagen GmbH | Verfahren zur Isolierung von Nukleinsäuren, wobei die Nukleinsäuren bei erhöhter Temperatur an einer Matrix immobilisiert werden |
| US20100311041A1 (en) | 2005-06-14 | 2010-12-09 | Statens Serum Institut | Pcr diagnostics of dermatophytes and other pathogenic fungi |
| US20070050311A1 (en) * | 2005-08-30 | 2007-03-01 | Caterpillar Inc. | Assembly should cost application |
| EP1945810B1 (en) * | 2005-09-20 | 2015-07-01 | AdvanDx, Inc. | Reagents, methods and kits for classification of fungi and direction of anti-fungal therapy |
| JP2007159411A (ja) | 2005-12-09 | 2007-06-28 | Canon Inc | プローブセット、プローブ固定担体及び遺伝子検査方法 |
| WO2007101664A2 (de) * | 2006-03-08 | 2007-09-13 | Fungus Bioscience Gmbh | Verbesserter aspergillosetest in klinischen proben |
| RU2348695C2 (ru) | 2006-05-23 | 2009-03-10 | Закрытое акционерное общество "Молекулярно-медицинские технологии" | Дифференцирующий и специфический олигонуклеотиды для идентификации последовательностей днк инфекционных агентов в биологических материалах, способ видовой идентификации инфекционных агентов, биочип и набор для осуществления этого способа |
| JP5015518B2 (ja) * | 2006-08-04 | 2012-08-29 | 東洋鋼鈑株式会社 | ハウスダストアレルギーの原因となるダニ・カビ類の検出・識別用マイクロアレイ |
| PT103729A (pt) * | 2007-05-04 | 2008-11-04 | Stab Vida Investigacao E Servi | Estojo e processo para a detecção e identificação de leveduras de interesse clínico, através de uma amplificação isotérmica de adn seguida de hibridação com oligonucleótidos específicos, e respectivas utilizações. |
| JP5196847B2 (ja) * | 2007-05-14 | 2013-05-15 | キヤノン株式会社 | プローブセット、プローブ担体及び検査方法 |
| JP5196850B2 (ja) * | 2007-05-14 | 2013-05-15 | キヤノン株式会社 | プローブセット、プローブ担体及び検査方法 |
| JP5196843B2 (ja) | 2007-05-14 | 2013-05-15 | キヤノン株式会社 | プローブセット、プローブ担体及び検査方法 |
| JP5317430B2 (ja) * | 2007-05-14 | 2013-10-16 | キヤノン株式会社 | プローブセット、プローブ担体、及び真菌の判別同定方法 |
| JP5196866B2 (ja) * | 2007-05-14 | 2013-05-15 | キヤノン株式会社 | プローブセット、プローブ担体及び検査方法 |
| JP5196845B2 (ja) * | 2007-05-14 | 2013-05-15 | キヤノン株式会社 | プローブセット、プローブ担体及び検査方法 |
| JP5196846B2 (ja) * | 2007-05-14 | 2013-05-15 | キヤノン株式会社 | プローブセット、プローブ担体、検査方法及びdna検出用キット |
| JP5196856B2 (ja) * | 2007-05-14 | 2013-05-15 | キヤノン株式会社 | プローブセット、プローブ担体及び検査方法 |
| JP5196861B2 (ja) | 2007-05-14 | 2013-05-15 | キヤノン株式会社 | プローブセット、プローブ担体及び検査方法 |
| JP5196853B2 (ja) * | 2007-05-14 | 2013-05-15 | キヤノン株式会社 | プローブセット、プローブ担体及び検査方法 |
| JP5196859B2 (ja) * | 2007-05-14 | 2013-05-15 | キヤノン株式会社 | プローブセット、プローブ担体、検査方法及びdna検出用キット |
| JP5196865B2 (ja) * | 2007-05-14 | 2013-05-15 | キヤノン株式会社 | プローブセット、プローブ担体及び検査方法 |
| JP5196860B2 (ja) * | 2007-05-14 | 2013-05-15 | キヤノン株式会社 | プローブセット、プローブ担体及び検査方法 |
| JP5196858B2 (ja) * | 2007-05-14 | 2013-05-15 | キヤノン株式会社 | プローブセット、プローブ担体、検査方法及びdna検出用キット |
| JP5196854B2 (ja) * | 2007-05-14 | 2013-05-15 | キヤノン株式会社 | プローブセット、プローブ担体及び検査方法 |
| US7829735B2 (en) * | 2007-10-26 | 2010-11-09 | Northwestern University | Universal phosphoramidite for preparation of modified biomolecules and surfaces |
| CA2715991A1 (en) | 2007-12-26 | 2009-07-09 | Gen-Probe Incorporated | Amplification oligomers and methods to detect candida albicans 26s rrna or encoding dna |
| CA2720292A1 (en) * | 2008-04-01 | 2009-10-08 | Metametrix Clinical Laboratory | Process and method for monitoring gastrointestinal microbiota |
| ES2335179B1 (es) * | 2008-09-19 | 2011-09-14 | Instituto De Salud Carlos Iii | Metodo para la deteccion simultanea de histoplasma capsulatum y paracoccidioides brasiliensis. |
| MX352246B (es) | 2009-03-18 | 2017-10-25 | Instituto Potosino De Investig Cientifica Y Tecnologica A C | Metodo in vitro para la deteccion de candida glabrata, kit de diagnostico y usos de los mismos. |
| PL2536850T3 (pl) | 2010-02-15 | 2017-09-29 | Council Of Scientific & Industrial Research | Sposób wykrywania patogenów grzybiczych |
| US10214780B2 (en) | 2012-03-09 | 2019-02-26 | City University Of Hong Kong | Method and means for identification of animal species |
| EP2636753B1 (en) * | 2012-03-09 | 2018-07-25 | City University of Hong Kong | Method and means for identification of animal species |
| US10253353B2 (en) * | 2013-12-06 | 2019-04-09 | The Broad Institute, Inc. | Enhanced methods of ribonucleic acid hybridization |
| CN105420372A (zh) * | 2015-12-17 | 2016-03-23 | 安徽出入境检验检疫局检验检疫技术中心 | 检测梨黑斑病菌的pcr引物以及使用该引物的检测方法 |
| CN107130015B (zh) * | 2016-02-29 | 2023-07-11 | 上海翔琼生物技术有限公司 | 一种检测烟曲霉菌的荧光定量pcr试剂盒 |
| CN106497796A (zh) * | 2016-07-20 | 2017-03-15 | 辽宁省微生物科学研究院 | 一种食用菌菌种的单克隆高效选育方法 |
| CN112626263B (zh) * | 2021-01-20 | 2022-08-05 | 中国人民解放军总医院 | 酶切探针恒温检测呼吸道感染真菌病原体的试剂盒 |
| CN112941231A (zh) * | 2021-04-26 | 2021-06-11 | 南方海洋科学与工程广东省实验室(湛江) | 一种用于白色念珠菌检测的探针、方法及应用 |
| EP4577673A1 (en) * | 2022-08-26 | 2025-07-02 | Denka Company Limited | Methods for the detection and identification of fungal species |
Family Cites Families (21)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US546027A (en) * | 1895-09-10 | Edgar charles parker | ||
| US4617102A (en) * | 1985-03-21 | 1986-10-14 | Lifecodes Corp. | Process and apparatus for purifying and concentrating DNA from crude mixtures containing DNA |
| US5541308A (en) * | 1986-11-24 | 1996-07-30 | Gen-Probe Incorporated | Nucleic acid probes for detection and/or quantitation of non-viral organisms |
| AU7429591A (en) * | 1990-04-18 | 1991-10-24 | Gene-Trak Systems | Nucleic acid probes for the detection of giardia lamblia |
| DE69332202T2 (de) | 1992-05-18 | 2003-05-28 | Richard David Cannon | Methoden zur diagnose von pilzinfektionen |
| US5426027A (en) | 1993-05-20 | 1995-06-20 | The Government Of The United States Of America As Represented By The Secretary | Nucleic acid probes and methods for detecting Candida DNA cells in blood |
| US6017699A (en) * | 1993-09-15 | 2000-01-25 | The University Of Pittsburgh | PCR identification and quantification of important Candida species |
| US5585238A (en) * | 1994-04-25 | 1996-12-17 | Ciba-Geigy Corporation | Detection of fungal pathogens using the polymerase chain reaction |
| US5763169A (en) * | 1995-01-13 | 1998-06-09 | Chiron Diagnostics Corporation | Nucleic acid probes for the detection and identification of fungi |
| US5693501A (en) | 1995-03-08 | 1997-12-02 | Indiana University Advanced Research & Technology Institute | Compounds and methods to determine presence of Histoplasma capsulatum |
| EP0846186B1 (de) | 1995-08-17 | 2008-02-20 | Myconostica Limited | Extraktion, amplifikation und sequentielle hybridisierung von pilzzellen-dna sowie verfahren zum nachweisen von pilzzellen in klinischem material |
| FR2749011B1 (fr) | 1996-03-22 | 1998-08-28 | Univ Claude Bernard Lyon | Sonde, sequence nucleiques et procede notamment, de detection et d'identification de levures, de meme que les outils genetiques correspondants |
| US5874221A (en) | 1996-08-28 | 1999-02-23 | The United States Of America As Represented By The Secretary Of Agriculture | Species specific method for the PCR detection of phythophthora |
| US6235890B1 (en) * | 1996-09-16 | 2001-05-22 | The United States Of America As Represented By The Department Of Health And Human Services | Methods and compositions for the detection of Candida spp. |
| US5814453A (en) | 1996-10-15 | 1998-09-29 | Novartis Finance Corporation | Detection of fungal pathogens using the polymerase chain reaction |
| US5800997A (en) | 1996-11-01 | 1998-09-01 | Novartis Finance Corporation | Detection of maize fungal pathogens using the polymerase chain reaction |
| ATE320506T1 (de) * | 1997-05-02 | 2006-04-15 | Us Gov Health & Human Serv | Nukleinsäuren zur erkennung von aspergillus spezien |
| US6242178B1 (en) * | 1997-07-30 | 2001-06-05 | Centers For Disease Control And Prevention | Nucleic acid probes for detecting Candida species |
| US5827695A (en) | 1997-08-04 | 1998-10-27 | Novartis Finance Corporation | Detection of wheat fungal pathogens using the polymerase chain reaction |
| US6080543A (en) | 1997-12-08 | 2000-06-27 | E. & J. Gallo Winery | Detection of fungal pathogens |
| JP4963765B2 (ja) * | 1999-05-28 | 2012-06-27 | イノジェネティックス・ナムローゼ・フェンノートシャップ | 臨床的に重要な真菌病原体を検出するための核酸プローブおよび方法 |
-
2000
- 2000-05-24 JP JP2001500809A patent/JP4963765B2/ja not_active Expired - Lifetime
- 2000-05-24 WO PCT/EP2000/004714 patent/WO2000073499A2/en not_active Ceased
- 2000-05-24 AU AU55255/00A patent/AU782305B2/en not_active Ceased
- 2000-05-24 CA CA2374878A patent/CA2374878C/en not_active Expired - Lifetime
- 2000-05-24 EP EP00940266.0A patent/EP1185702B1/en not_active Expired - Lifetime
- 2000-05-24 ES ES00940266.0T patent/ES2621547T3/es not_active Expired - Lifetime
- 2000-09-15 US US09/662,462 patent/US6858387B1/en not_active Expired - Lifetime
-
2004
- 2004-10-19 US US10/967,254 patent/US7741461B2/en not_active Expired - Fee Related
-
2012
- 2012-01-26 JP JP2012014151A patent/JP2012120535A/ja active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| EP1185702B1 (en) | 2017-02-08 |
| ES2621547T3 (es) | 2017-07-04 |
| US20050164243A1 (en) | 2005-07-28 |
| JP4963765B2 (ja) | 2012-06-27 |
| JP2012120535A (ja) | 2012-06-28 |
| WO2000073499A2 (en) | 2000-12-07 |
| WO2000073499A3 (en) | 2001-08-09 |
| US6858387B1 (en) | 2005-02-22 |
| EP1185702A2 (en) | 2002-03-13 |
| JP2003501049A (ja) | 2003-01-14 |
| AU5525500A (en) | 2000-12-18 |
| AU782305B2 (en) | 2005-07-21 |
| CA2374878A1 (en) | 2000-12-07 |
| US7741461B2 (en) | 2010-06-22 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CA2374878C (en) | Nucleic acid probes and methods for detecting clinically important fungal pathogens | |
| JP2003501049A5 (enExample) | ||
| Kurtzman et al. | Identification of clinically important ascomycetous yeasts based on nucleotide divergence in the 5'end of the large-subunit (26S) ribosomal DNA gene | |
| US5635353A (en) | Nucleic acid probes and methods for detecting Candida krusei cells in blood | |
| AU720037B2 (en) | Methods and compositions for the detection of Candida spp. | |
| Leaw et al. | Identification of medically important Candida and non-Candida yeast species by an oligonucleotide array | |
| WO1998055649A1 (en) | Nucleic acid probes for the detection and identification of fungi | |
| EP1062365A1 (en) | Detection of fermentation-related microorganisms | |
| Aguilera et al. | A molecular approach to the characterization of the eukaryotic communities of an extreme acidic environment: methods for DNA extraction and denaturing gradient gel electrophoresis analysis | |
| AU2006294692B2 (en) | Compositions and methods for the detection of candida species | |
| WO2008103015A1 (en) | Methods, compositions and kits for increasing efficiency or specificity of hybridization between pna probes immobilized on support and target nucleic acids | |
| US5693501A (en) | Compounds and methods to determine presence of Histoplasma capsulatum | |
| JP4499836B2 (ja) | 食品を汚染する可能性のある耐熱性微生物の検出方法 | |
| US6046006A (en) | Sequential hybridization of fungal cell DNA and method for the detection of fungal cells in clinical material | |
| WO2001053316A1 (en) | Detection of fermentation-related microorganisms | |
| EP0860503A1 (en) | Material and method for detecting fungi | |
| Gupta et al. | Exploratory study of single-copy genes and ribosomal intergenic spacers for rapid distinction of dermatophytes | |
| US7465543B1 (en) | Multiplex DNA identification of clinical yeasts using flow cytometry | |
| EP1682674A2 (en) | Methods for monitoring gene expression in filamentous fungal cells | |
| Lott et al. | Nucleic acid probes and methods for detecting Candida DNA cells in blood | |
| US20020098487A1 (en) | Materials and methods for detecting fungi | |
| JP2002142774A (ja) | 真菌検出用核酸及びそれを用いた真菌の検出方法 | |
| US20080096198A1 (en) | Primer Having Promoter Region Added Thereto | |
| Engelke | Genome-wide search for RNase P substrates reveals nuclear RNase P is involved in maturation of intronencoded box C/D small nucleolar RNAs | |
| JPWO1998010073A1 (ja) | 真菌類の検出用材料及び検出法 |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EEER | Examination request | ||
| MKEX | Expiry |
Effective date: 20200524 |