ES2604329T3 - Enzimas que tienen actividad de alfa amilasa y métodos de uso de las mismas - Google Patents
Enzimas que tienen actividad de alfa amilasa y métodos de uso de las mismas Download PDFInfo
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- ES2604329T3 ES2604329T3 ES10184415.7T ES10184415T ES2604329T3 ES 2604329 T3 ES2604329 T3 ES 2604329T3 ES 10184415 T ES10184415 T ES 10184415T ES 2604329 T3 ES2604329 T3 ES 2604329T3
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
- C12N9/2411—Amylases
- C12N9/2414—Alpha-amylase (3.2.1.1.)
- C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source
-
- A—HUMAN NECESSITIES
- A21—BAKING; EDIBLE DOUGHS
- A21D—TREATMENT OF FLOUR OR DOUGH FOR BAKING, e.g. BY ADDITION OF MATERIALS; BAKING; BAKERY PRODUCTS
- A21D8/00—Methods for preparing or baking dough
- A21D8/02—Methods for preparing dough; Treating dough prior to baking
- A21D8/04—Methods for preparing dough; Treating dough prior to baking treating dough with microorganisms or enzymes
- A21D8/042—Methods for preparing dough; Treating dough prior to baking treating dough with microorganisms or enzymes with enzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
- C12N9/2411—Amylases
- C12N9/2414—Alpha-amylase (3.2.1.1.)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01001—Alpha-amylase (3.2.1.1)
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Food Science & Technology (AREA)
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Bakery Products And Manufacturing Methods Therefor (AREA)
- Cereal-Derived Products (AREA)
- Fodder In General (AREA)
- Paper (AREA)
- Alcoholic Beverages (AREA)
- Detergent Compositions (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Chemical Or Physical Treatment Of Fibers (AREA)
- Jellies, Jams, And Syrups (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Applications Claiming Priority (6)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US27049501P | 2001-02-21 | 2001-02-21 | |
| US27049601P | 2001-02-21 | 2001-02-21 | |
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Families Citing this family (100)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20040005673A1 (en) * | 2001-06-29 | 2004-01-08 | Kevin Jarrell | System for manipulating nucleic acids |
| AU774306B2 (en) * | 1999-01-05 | 2004-06-24 | Trustees Of Boston University | Improved nucleic acid cloning |
| AU2001277014A1 (en) * | 2000-07-21 | 2002-02-05 | Trustees Of Boston University | Modular vector systems |
| US7435562B2 (en) | 2000-07-21 | 2008-10-14 | Modular Genetics, Inc. | Modular vector systems |
| MXPA03000793A (es) | 2000-07-28 | 2003-06-04 | Henkel Kgaa | Enzima amilolitica novedosa, extraida de bacilo sp a 7-7 (dsm 12368) y agentes de lavado y limpiadores que contienen esta enzima amilolitica novedosa. |
| US7659102B2 (en) | 2001-02-21 | 2010-02-09 | Verenium Corporation | Amylases, nucleic acids encoding them and methods for making and using them |
| CA2438205C (en) | 2001-02-21 | 2015-11-03 | Diversa Corporation | Enzymes having alpha amylase activity and methods of use thereof |
| US7560126B2 (en) | 2001-02-21 | 2009-07-14 | Verenium Corporation | Amylases, nucleic acids encoding them and methods for making and using them |
| DE10138753B4 (de) * | 2001-08-07 | 2017-07-20 | Henkel Ag & Co. Kgaa | Wasch- und Reinigungsmittel mit Hybrid-Alpha-Amylasen |
| RU2312144C2 (ru) | 2001-08-27 | 2007-12-10 | Синджента Партисипейшнс Аг | Аутопроцессирующиеся растения и части растений |
| WO2004042006A2 (en) | 2002-10-31 | 2004-05-21 | Diversa Corporation | Amylases, nucleic acids encoding them and methods for making and using them |
| ATE347591T1 (de) | 2002-12-17 | 2006-12-15 | Novozymes As | Thermostabile alpha-amylase |
| EP3508578A1 (en) * | 2003-03-06 | 2019-07-10 | BASF Enzymes, LLC | Amylases, nucleic acids encoding them and methods for making and using them |
| AU2015218541A1 (en) * | 2003-03-06 | 2015-10-29 | Basf Enzymes Llc | Amylases, nucleic acids encoding them and methods for making and using them |
| JP4778418B2 (ja) | 2003-03-06 | 2011-09-21 | ヴェレニウム コーポレイション | アミラーゼ、それをコードする核酸並びにその製造および使用方法 |
| WO2004081022A2 (en) * | 2003-03-10 | 2004-09-23 | Genencor International Inc. | Grain compositions containing pre-biotic isomalto-oligosaccharides and methods of making and using same |
| DE602004026733D1 (de) | 2003-06-13 | 2010-06-02 | Danisco | Pseudomonas-polypeptidvarianten mit einer nicht maltogenen exoamylaseaktivität und ihre verwendung bei der herstellung von lebensmittelprodukten |
| CA2538349C (en) | 2003-06-25 | 2014-08-12 | Novozymes A/S | Polypeptides having alpha-amylase activity and polynucleotides encoding same |
| US7618795B2 (en) | 2003-06-25 | 2009-11-17 | Novozymes A/S | Starch process |
| US8105801B2 (en) | 2003-06-25 | 2012-01-31 | Novozymes A/S | Starch process |
| US8143048B2 (en) | 2003-07-07 | 2012-03-27 | Danisco A/S | Exo-specific amylase polypeptides, nucleic acids encoding those polypeptides and uses thereof |
| EP1644514A4 (en) * | 2003-07-07 | 2009-05-27 | Genencor Int | TEMPERATURE STABILIZED AMYLASE POLYPEPTIDES, NUCLEIC ACIDS CODING THEREOF, AND USES THEREOF |
| US7169257B2 (en) * | 2003-11-12 | 2007-01-30 | Kemira Chemicals, Inc. | Method of deinking waste paper using a reduced alkali system |
| BRPI0418622B1 (pt) * | 2004-03-08 | 2020-01-28 | Syngenta Participations Ag | polinucleotídeo isolado, cassete de expressão e método para preparar açúcar fermentável, monossacarídeo ou oligossacarídeo |
| US20050257932A1 (en) * | 2004-05-19 | 2005-11-24 | Davidson Eric A | Filter cake degradation compositions and associated methods |
| AU2011203198B2 (en) * | 2004-07-07 | 2013-01-10 | Dupont Nutrition Biosciences Aps | Polypeptide |
| MX2007000334A (es) * | 2004-07-07 | 2007-06-25 | Danisco | Polipeptido. |
| US20060084150A1 (en) * | 2004-09-29 | 2006-04-20 | Qunyu Gao | Method for manufacturing maltose-rich products |
| JP2008519602A (ja) * | 2004-11-11 | 2008-06-12 | モジュラー ジェネティクス, インコーポレイテッド | 多様性を生じるためのラダーアセンブリおよびシステム |
| DE602005025038D1 (de) | 2004-12-22 | 2011-01-05 | Novozymes As | Seaminosäuresequenz und einem kohlenhydratbindenden modul als zweiter aminosäuresequenz |
| US20060147581A1 (en) | 2004-12-22 | 2006-07-06 | Novozymes A/S | Hybrid enzymes |
| CA2599381C (en) * | 2005-03-16 | 2013-10-22 | Syngenta Participations Ag | Corn event 3272 and methods for detection thereof |
| US20070184472A1 (en) * | 2005-06-08 | 2007-08-09 | Toagosei Co., Ltd | Method of purifying environmental dna and method of efficiently screening for protein-encoding gene from environmental dna |
| US8030050B2 (en) | 2005-07-07 | 2011-10-04 | Danisco A/S | Modified amylases from Pseudomonas species |
| CN105296450A (zh) | 2006-06-19 | 2016-02-03 | 杜邦营养生物科学有限公司 | 多肽 |
| US20080014616A1 (en) * | 2006-07-11 | 2008-01-17 | Kevin Jarrell | Methods of introducing targeted diversity into nucleic acid molecules |
| MX369001B (es) | 2006-08-04 | 2019-10-24 | Basf Enzymes Llc | Glucanasas, acidos nucleicos que las codifican, y metodos para hacerlas y usarlas. |
| US7808342B2 (en) * | 2006-10-02 | 2010-10-05 | Skyworks Solutions, Inc. | Harmonic phase tuning filter for RF switches |
| AU2007336732B2 (en) | 2006-12-21 | 2013-05-02 | Syngenta Participations Ag | Amylases and glucoamylases, nucleic acids encoding them and methods for making and using them |
| US7727726B2 (en) * | 2007-06-01 | 2010-06-01 | Syngenta Participations Ag | Process for starch liquefaction and fermentation |
| WO2008150880A1 (en) * | 2007-06-01 | 2008-12-11 | Syngenta Participations Ag | Process for starch liquefaction and fermentation |
| US7915020B2 (en) * | 2007-06-01 | 2011-03-29 | Syngenta Participations Ag | Process for starch liquefaction and fermentation |
| CN102943098A (zh) | 2008-01-02 | 2013-02-27 | 丹尼斯科美国公司 | 应用嗜糖假单胞菌g4-淀粉酶和其变体获得乙醇的无葡糖淀粉酶的方法 |
| US9816119B2 (en) | 2008-02-29 | 2017-11-14 | Syngenta Participations Ag | Methods for starch hydrolysis |
| US9040278B2 (en) | 2008-06-06 | 2015-05-26 | Danisco Us Inc. | Production of glucose from starch using alpha-amylases from Bacillus subtilis |
| MX2010013123A (es) | 2008-06-06 | 2011-01-14 | Danisco Inc | Alfa-amilasas variantes de bacillus subtilis y metodos de uso de las mismas. |
| BRPI0915531A2 (pt) | 2008-06-06 | 2017-08-29 | Danisco Us Inc | Composição de enzima de sacarificação e método de sacarificação da mesma |
| US7666457B1 (en) | 2008-08-19 | 2010-02-23 | Delavau Llc | Dry mixes comprising glycerine |
| US20120172275A1 (en) * | 2009-03-10 | 2012-07-05 | Danisco Us Inc. | Bacillus Megaterium Strain DSM90-Related Alpha-Amylases, and Methods of Use, Thereof |
| MX2011010040A (es) * | 2009-04-01 | 2011-11-18 | Danisco Us Inc | Sistema de limpieza que comprende una alfa-amilasa y una proteasa. |
| DK3412771T3 (da) * | 2009-05-19 | 2021-01-18 | Dupont Nutrition Biosci Aps | Amylasepolypeptider |
| MX2012001243A (es) | 2009-08-07 | 2012-03-26 | Danisco Us Inc | Combinacion de alfa-amilasa para procesamiento de almidon y metodo de uso de la misma. |
| BR112012009659A2 (pt) * | 2009-10-23 | 2015-09-15 | Danisco Us Inc | métodos para redução de sacarídeo azul. |
| EP2510092A1 (en) | 2009-12-09 | 2012-10-17 | The Procter & Gamble Company | Fabric and home care products |
| EP2529012A2 (en) * | 2010-01-25 | 2012-12-05 | Syngenta Participations AG | Compositions and methods relating to dual activity enzymes having xylanase and cellulase activity |
| MX351850B (es) * | 2011-06-30 | 2017-10-31 | Novozymes As | Metodo para el cribado de alfa-amilasas. |
| US20130159253A1 (en) * | 2011-12-15 | 2013-06-20 | Sybase, Inc. | Directing a data replication environment through policy declaration |
| US9672126B2 (en) | 2011-12-15 | 2017-06-06 | Sybase, Inc. | Hybrid data replication |
| GB2499463B (en) * | 2012-01-31 | 2014-04-02 | Verenium Corp | Reduced sugar syrups and methods of making reduced sugar syrups |
| WO2014007921A1 (en) | 2012-06-08 | 2014-01-09 | Danisco Us Inc. | Variant alpha amylases with enhanced activity on starch polymers |
| WO2014150022A1 (en) | 2013-03-15 | 2014-09-25 | Grain Processing Corporation | Preparation of malto-oligosaccharides |
| US20160186102A1 (en) * | 2013-10-03 | 2016-06-30 | Danisco Us Inc. | Alpha-amylases from exiguobacterium, and methods of use, thereof |
| CN107529787A (zh) | 2015-04-10 | 2018-01-02 | 先正达参股股份有限公司 | 动物饲料组合物及使用方法 |
| CN108138447A (zh) | 2015-08-14 | 2018-06-08 | 巴斯夫欧洲公司 | 用于纸和纸板的含水表面处理组合物 |
| FR3045055B1 (fr) | 2015-12-10 | 2020-02-21 | Roquette Freres | Hydrolysat d'amidon de basse viscosite presentant un comportement a la retrogradation ameliore |
| US20190100738A1 (en) * | 2015-12-18 | 2019-04-04 | Basf Enzymes Llc | A Liquid Formulation of Alpha-Amylase |
| DE102016208466A1 (de) | 2016-05-18 | 2017-11-23 | Henkel Ag & Co. Kgaa | Verbesserte Waschleistung durch eine neue alpha-Amylase aus Rhizoctonia solani |
| DE102016209880A1 (de) | 2016-06-06 | 2017-12-07 | Henkel Ag & Co. Kgaa | Neue Amylasen |
| WO2017220787A1 (en) * | 2016-06-24 | 2017-12-28 | Evaxion Biotech Aps | Vaccines against aearomonas salmonicida infection |
| CN106011107B (zh) * | 2016-06-30 | 2019-09-10 | 安徽工程大学 | 一种耐碱性α-淀粉酶及其基因工程菌的构建方法和应用 |
| US20190203413A1 (en) | 2016-09-16 | 2019-07-04 | Basf Se | Methods of Modifying Pulp Comprising Cellulase Enzymes and Products Thereof |
| DE102016221851A1 (de) | 2016-11-08 | 2018-05-09 | Henkel Ag & Co. Kgaa | Amylase für Wasch- und Reinigungsmittelanwendungen |
| US12110531B2 (en) | 2017-06-16 | 2024-10-08 | Danstar Ferment Ag | Method for increasing oil yield during ethanol production |
| WO2019075028A1 (en) | 2017-10-12 | 2019-04-18 | Syngenta Participations Ag | ENHANCED ANIMAL FEEDING COMPOSITIONS AND METHODS OF USE |
| CA3076578A1 (en) | 2017-10-25 | 2019-05-02 | Basf Se | Beta-amylase enzymes |
| DE102017220757A1 (de) | 2017-11-21 | 2019-05-23 | Henkel Ag & Co. Kgaa | Amylase und eine solche enthaltendes Wasch- oder Reinigungsmittel |
| US20230091532A1 (en) * | 2018-03-13 | 2023-03-23 | Lallemand Hungary Liquidity Management Llc | Inactivated yeast and yeast product for improving fermentation yield |
| WO2019175807A1 (en) * | 2018-03-13 | 2019-09-19 | Lallemand Hungary Liquidity Management Llc | Yeast expressing thermostable alpha-amylases for hydrolysis of starch |
| BR112020021692A2 (pt) | 2018-04-26 | 2021-01-26 | Basf Se | polipeptídeo tendo atividade de lipase, polipeptídeo híbrido, composição, polinucleotídeo variante, método para fabricar a variante de polipeptídeo, e, uso do polipeptídeo |
| EP3788145A1 (en) | 2018-05-03 | 2021-03-10 | Basf Se | Amylase enzymes |
| EP3802844A1 (en) * | 2018-05-25 | 2021-04-14 | Basf Se | Uses of surfactants in starch processing |
| US12351841B2 (en) | 2018-05-29 | 2025-07-08 | Basf Se | Amylase enzymes |
| DE102018208443A1 (de) | 2018-05-29 | 2019-12-05 | Henkel Ag & Co. Kgaa | Verbesserte Waschleistung durch eine neue alpha-Amylase Irpex lacteus (IIa) |
| DE102018208445A1 (de) | 2018-05-29 | 2019-12-05 | Henkel Ag & Co. Kgaa | Verbesserte Waschleistung durch eine neue alpha-Amylase aus Fomitopsis pinicola (Fpi) |
| DE102018208444A1 (de) | 2018-05-29 | 2019-12-05 | Henkel Ag & Co. Kgaa | Verbesserte Waschleistung durch eine neue alpha-Amylase aus Trametes hirsuta (Thi) |
| DE102018208446A1 (de) | 2018-05-29 | 2019-12-05 | Henkel Ag & Co. Kgaa | Verbesserte Waschleistung durch eine neue alpha-Amylase aus Fomes fomentarius (Ffo) |
| US20220186234A1 (en) | 2019-02-20 | 2022-06-16 | Basf Se | Industrial Fermentation Process for Bacillus Using Defined Medium and Trace Element Feed |
| MX2021010110A (es) | 2019-02-20 | 2021-09-21 | Basf Se | Proceso de fermentacion industrial para bacillus mediante el uso de medio definido y alimentacion de magnesio. |
| EP3938504A1 (en) | 2019-03-11 | 2022-01-19 | Basf Se | Amylases and methods for making and using them |
| EP3947664A2 (en) | 2019-03-25 | 2022-02-09 | Basf Se | Amylase enzymes |
| WO2020193532A1 (en) | 2019-03-25 | 2020-10-01 | Basf Se | Cleaning composition having amylase enzymes |
| EP3947665A2 (en) | 2019-03-25 | 2022-02-09 | Basf Se | Amylase enzymes |
| CN118086254A (zh) | 2019-04-23 | 2024-05-28 | 巴斯夫欧洲公司 | β-淀粉酶变体 |
| GB2595998B8 (en) | 2019-05-13 | 2023-03-08 | Halliburton Energy Services Inc | Injectivity and production improvement in oil and gas fields |
| US12232512B2 (en) * | 2019-06-05 | 2025-02-25 | Danisco Us Inc. | Methods for improving the amino acid content of animal feed products |
| MX2021015364A (es) | 2019-06-13 | 2022-01-24 | Basf Se | Metodo de recuperacion de una proteina de un caldo de fermentacion mediante el uso de un cation divalente. |
| WO2021004830A1 (en) | 2019-07-05 | 2021-01-14 | Basf Se | Industrial fermentation process for microbial cells using a fed-batch pre-culture |
| CN114364795A (zh) | 2019-08-22 | 2022-04-15 | 巴斯夫欧洲公司 | 淀粉酶变体 |
| EP4525615A2 (en) | 2022-05-14 | 2025-03-26 | Novozymes A/S | Compositions and methods for preventing, treating, supressing and/or eliminating phytopathogenic infestations and infections |
| CN119060988B (zh) * | 2024-09-30 | 2025-08-22 | 南京大学 | 一种耐盐的α-淀粉酶突变体及其应用 |
Family Cites Families (50)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3847740A (en) * | 1972-10-02 | 1974-11-12 | Cpc International Inc | Process for the production of levulose-bearing syrups |
| JPS5823799A (ja) | 1981-08-03 | 1983-02-12 | 株式会社林原生物化学研究所 | 高純度マルト−スの製造方法 |
| JPS5872598A (ja) * | 1981-10-26 | 1983-04-30 | Hayashibara Biochem Lab Inc | 高純度イソマルト−スの製造方法 |
| US4557927A (en) * | 1983-03-10 | 1985-12-10 | Kabushiki Kaisha Hoyashibara | Food products and process for producing same |
| DE3688920D1 (de) | 1985-07-03 | 1993-09-30 | Genencor Int | Hybride Polypeptide und Verfahren zu deren Herstellung. |
| JPS62104580A (ja) | 1985-10-30 | 1987-05-15 | Kunio Yamane | 高耐熱性酵素発現dna |
| FI93859C (fi) * | 1985-12-03 | 1995-06-12 | Gist Brocades Nv | Menetelmä glukoosisiirappien ja puhdistettujen tärkkelysten tuottamiseksi vehnän ja muiden viljakasvien pentosaaneja sisältävistä tärkkelyksistä |
| GB8609288D0 (en) * | 1986-04-16 | 1986-05-21 | Ici Plc | Debranching enzyme |
| DK311186D0 (da) | 1986-06-30 | 1986-06-30 | Novo Industri As | Enzymer |
| US4946778A (en) | 1987-09-21 | 1990-08-07 | Genex Corporation | Single polypeptide chain binding molecules |
| JP2696537B2 (ja) | 1988-10-28 | 1998-01-14 | 東和化成工業株式会社 | 高純度マルトースの製造方法 |
| WO1991016427A1 (en) | 1990-04-24 | 1991-10-31 | Stratagene | Methods for phenotype creation from multiple gene populations |
| US6582908B2 (en) * | 1990-12-06 | 2003-06-24 | Affymetrix, Inc. | Oligonucleotides |
| EP0599859A1 (en) | 1991-06-25 | 1994-06-08 | Novo Nordisk A/S | Mammalian pancreatic lipase and variant thereof |
| DK0691344T3 (da) | 1992-12-28 | 2003-07-14 | Hayashibara Biochem Lab | Oprensning af trehalose |
| US6297037B1 (en) | 1993-02-11 | 2001-10-02 | Christopher C. Barnett | Oxidatively stable alpha-amylase |
| JP3559585B2 (ja) | 1993-06-03 | 2004-09-02 | 株式会社林原生物化学研究所 | トレハロース遊離酵素とその製造方法並びに用途 |
| EP0648843A1 (en) | 1993-10-01 | 1995-04-19 | Takara Shuzo Co. Ltd. | DNA encoding a hyperthermostable alpha-amylase |
| JPH07143880A (ja) * | 1993-10-01 | 1995-06-06 | Takara Shuzo Co Ltd | 超耐熱性α−アミラーゼ遺伝子 |
| EP0722490B2 (en) * | 1993-10-08 | 2013-10-23 | Novozymes A/S | Amylase variants |
| JPH11511118A (ja) | 1995-06-02 | 1999-09-28 | ノボ ノルディスク アクティーゼルスカブ | タンパク質溶液のA1/Fe処理とその後の膜濃縮 |
| US5736499A (en) | 1995-06-06 | 1998-04-07 | Genencor International, Inc. | Mutant A-amylase |
| JPH09173077A (ja) * | 1995-07-20 | 1997-07-08 | Tadayuki Imanaka | 超耐熱性酸性α−アミラーゼおよび該α−アミラーゼ産生遺伝子を含むDNA断片 |
| US5965408A (en) | 1996-07-09 | 1999-10-12 | Diversa Corporation | Method of DNA reassembly by interrupting synthesis |
| US6479258B1 (en) * | 1995-12-07 | 2002-11-12 | Diversa Corporation | Non-stochastic generation of genetic vaccines |
| US5939250A (en) | 1995-12-07 | 1999-08-17 | Diversa Corporation | Production of enzymes having desired activities by mutagenesis |
| CN1222938A (zh) * | 1996-05-14 | 1999-07-14 | 金克克国际有限公司 | 经修饰具有改变了的钙离子吉合性质的α-淀粉酶 |
| US5789228A (en) * | 1996-05-22 | 1998-08-04 | Diversa Corporation | Endoglucanases |
| CN1233286A (zh) * | 1996-10-11 | 1999-10-27 | 诺沃挪第克公司 | a-淀粉酶与纤维素结合结构域融合以用于淀粉降解 |
| WO1998045417A1 (en) * | 1997-04-09 | 1998-10-15 | Michigan State University | HYPERTHERMOSTABLE α-AMYLASE |
| EP2386568B1 (en) * | 1997-10-30 | 2014-08-06 | Novozymes A/S | Alpha-amylase mutants |
| AUPP209298A0 (en) * | 1998-03-02 | 1998-03-26 | Uniphase Fibre Components Pty Limited | Grating writing techniques |
| FR2778412B1 (fr) * | 1998-05-05 | 2002-08-09 | Univ Reims Champagne Ardennes | Procede pour preparer une enzyme alpha-amylase thermophile et enzyme ainsi obtenue |
| RU2221870C2 (ru) | 1998-06-24 | 2004-01-20 | Авентис Фарма Дойчланд Гмбх | Мумбайстатин, способ его получения и его применение в качестве фармацевтического препарата |
| AU774004B2 (en) | 1998-06-25 | 2004-06-10 | Ventria Bioscience | Plant selectable marker and plant transformation method |
| CA2363551A1 (en) | 1999-03-25 | 2000-10-05 | Genset S.A. | Biallelic markers related to genes involved in drug metabolism |
| BRPI0009362B8 (pt) * | 1999-03-30 | 2019-08-20 | Novozymes As | variante de uma alfa-amilase precursora, e, uso de uma variante de alfa-amilase |
| TWI315741B (en) * | 2000-05-22 | 2009-10-11 | Hayashibara Biochem Lab | A-isomaltosyltransferase, process for producing the same and use thereof |
| US20020137153A1 (en) | 2000-10-04 | 2002-09-26 | Ramer Sandra W. | Enantioselective production of amino carboxylic acids |
| CA2438205C (en) | 2001-02-21 | 2015-11-03 | Diversa Corporation | Enzymes having alpha amylase activity and methods of use thereof |
| US7560126B2 (en) * | 2001-02-21 | 2009-07-14 | Verenium Corporation | Amylases, nucleic acids encoding them and methods for making and using them |
| US7659102B2 (en) * | 2001-02-21 | 2010-02-09 | Verenium Corporation | Amylases, nucleic acids encoding them and methods for making and using them |
| DE10131441A1 (de) | 2001-06-29 | 2003-01-30 | Henkel Kgaa | Eine neue Gruppe von alpha-Amylasen sowie ein Verfahren zur Identifizierung und Gewinnung neuer alpha-Amylasen |
| WO2003012071A2 (en) | 2001-08-03 | 2003-02-13 | Elitra Pharmaceuticals, Inc. | Nucleic acids of aspergillus fumigatus encoding industrial enzymes and methods of use |
| RU2312144C2 (ru) | 2001-08-27 | 2007-12-10 | Синджента Партисипейшнс Аг | Аутопроцессирующиеся растения и части растений |
| US20040025925A1 (en) * | 2002-08-06 | 2004-02-12 | Jeremy Gin | Adjustable mobility-aid apparatus that avoids triggering alarms |
| JP4778418B2 (ja) | 2003-03-06 | 2011-09-21 | ヴェレニウム コーポレイション | アミラーゼ、それをコードする核酸並びにその製造および使用方法 |
| EP2358873B2 (en) | 2008-12-15 | 2018-02-21 | Danisco US Inc. | Hybrid alpha-amylases |
| US8857799B2 (en) | 2011-05-12 | 2014-10-14 | Sealy Technology, Llc | Advanced conformance encased coil spring units |
| JP6449266B2 (ja) | 2013-06-21 | 2019-01-09 | バイオ−ラッド・ラボラトリーズ・インコーポレーテッド | 流体ピックアップを備えるマイクロ流体システム |
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