ES2536555T3 - Medio de reacción para la detección y/o la identificación de bacterias del género Legionela - Google Patents
Medio de reacción para la detección y/o la identificación de bacterias del género Legionela Download PDFInfo
- Publication number
- ES2536555T3 ES2536555T3 ES09756026.2T ES09756026T ES2536555T3 ES 2536555 T3 ES2536555 T3 ES 2536555T3 ES 09756026 T ES09756026 T ES 09756026T ES 2536555 T3 ES2536555 T3 ES 2536555T3
- Authority
- ES
- Spain
- Prior art keywords
- legionella
- reaction medium
- medium
- bacteria
- silica
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 241000589248 Legionella Species 0.000 title claims abstract description 44
- 208000007764 Legionnaires' Disease Diseases 0.000 title claims abstract description 39
- 239000012429 reaction media Substances 0.000 title claims abstract description 29
- 241000894006 Bacteria Species 0.000 title claims abstract description 18
- 238000001514 detection method Methods 0.000 title claims abstract description 13
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims abstract description 53
- 239000000377 silicon dioxide Substances 0.000 claims abstract description 32
- 150000003377 silicon compounds Chemical class 0.000 claims abstract description 12
- 239000000758 substrate Substances 0.000 claims description 36
- 239000000203 mixture Substances 0.000 claims description 20
- 102000004190 Enzymes Human genes 0.000 claims description 12
- 108090000790 Enzymes Proteins 0.000 claims description 12
- 238000000034 method Methods 0.000 claims description 10
- 108090000854 Oxidoreductases Proteins 0.000 claims description 9
- 102000004316 Oxidoreductases Human genes 0.000 claims description 9
- 108090000604 Hydrolases Proteins 0.000 claims description 4
- 102000004157 Hydrolases Human genes 0.000 claims description 4
- 239000002609 medium Substances 0.000 description 50
- 241000589242 Legionella pneumophila Species 0.000 description 24
- 238000011534 incubation Methods 0.000 description 16
- 229940115932 legionella pneumophila Drugs 0.000 description 13
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 description 10
- 239000004471 Glycine Substances 0.000 description 10
- 244000005700 microbiome Species 0.000 description 10
- 229920001817 Agar Polymers 0.000 description 9
- 239000008272 agar Substances 0.000 description 9
- -1 octadecyl silica Chemical compound 0.000 description 9
- 239000004113 Sepiolite Substances 0.000 description 8
- YPHMISFOHDHNIV-FSZOTQKASA-N cycloheximide Chemical compound C1[C@@H](C)C[C@H](C)C(=O)[C@@H]1[C@H](O)CC1CC(=O)NC(=O)C1 YPHMISFOHDHNIV-FSZOTQKASA-N 0.000 description 8
- RWSXRVCMGQZWBV-WDSKDSINSA-N glutathione Natural products OC(=O)[C@@H](N)CCC(=O)N[C@@H](CS)C(=O)NCC(O)=O RWSXRVCMGQZWBV-WDSKDSINSA-N 0.000 description 8
- 229910052624 sepiolite Inorganic materials 0.000 description 8
- 235000019355 sepiolite Nutrition 0.000 description 8
- 229940041514 candida albicans extract Drugs 0.000 description 7
- 238000002955 isolation Methods 0.000 description 7
- 239000000523 sample Substances 0.000 description 7
- 239000012138 yeast extract Substances 0.000 description 7
- 239000007991 ACES buffer Substances 0.000 description 6
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 6
- 210000004072 lung Anatomy 0.000 description 6
- VLSOAXRVHARBEQ-UHFFFAOYSA-N [4-fluoro-2-(hydroxymethyl)phenyl]methanol Chemical compound OCC1=CC=C(F)C=C1CO VLSOAXRVHARBEQ-UHFFFAOYSA-N 0.000 description 5
- 229940009533 alpha-ketoglutaric acid Drugs 0.000 description 5
- 239000003593 chromogenic compound Substances 0.000 description 5
- 150000001875 compounds Chemical class 0.000 description 5
- 239000011706 ferric diphosphate Substances 0.000 description 5
- 229940036404 ferric pyrophosphate Drugs 0.000 description 5
- 239000001963 growth medium Substances 0.000 description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 5
- YKJYKKNCCRKFSL-RDBSUJKOSA-N (-)-anisomycin Chemical compound C1=CC(OC)=CC=C1C[C@@H]1[C@H](OC(C)=O)[C@@H](O)CN1 YKJYKKNCCRKFSL-RDBSUJKOSA-N 0.000 description 4
- YKJYKKNCCRKFSL-UHFFFAOYSA-N Anisomycin Natural products C1=CC(OC)=CC=C1CC1C(OC(C)=O)C(O)CN1 YKJYKKNCCRKFSL-UHFFFAOYSA-N 0.000 description 4
- 240000007124 Brassica oleracea Species 0.000 description 4
- 235000003899 Brassica oleracea var acephala Nutrition 0.000 description 4
- 235000011301 Brassica oleracea var capitata Nutrition 0.000 description 4
- 235000001169 Brassica oleracea var oleracea Nutrition 0.000 description 4
- RWSXRVCMGQZWBV-PHDIDXHHSA-N L-Glutathione Natural products OC(=O)[C@H](N)CCC(=O)N[C@H](CS)C(=O)NCC(O)=O RWSXRVCMGQZWBV-PHDIDXHHSA-N 0.000 description 4
- 208000004023 Legionellosis Diseases 0.000 description 4
- 102100024295 Maltase-glucoamylase Human genes 0.000 description 4
- SBKRTALNRRAOJP-BWSIXKJUSA-N N-[(2S)-4-amino-1-[[(2S,3R)-1-[[(2S)-4-amino-1-oxo-1-[[(3S,6S,9S,12S,15R,18R,21S)-6,9,18-tris(2-aminoethyl)-15-benzyl-3-[(1R)-1-hydroxyethyl]-12-(2-methylpropyl)-2,5,8,11,14,17,20-heptaoxo-1,4,7,10,13,16,19-heptazacyclotricos-21-yl]amino]butan-2-yl]amino]-3-hydroxy-1-oxobutan-2-yl]amino]-1-oxobutan-2-yl]-6-methylheptanamide (6S)-N-[(2S)-4-amino-1-[[(2S,3R)-1-[[(2S)-4-amino-1-oxo-1-[[(3S,6S,9S,12S,15R,18R,21S)-6,9,18-tris(2-aminoethyl)-15-benzyl-3-[(1R)-1-hydroxyethyl]-12-(2-methylpropyl)-2,5,8,11,14,17,20-heptaoxo-1,4,7,10,13,16,19-heptazacyclotricos-21-yl]amino]butan-2-yl]amino]-3-hydroxy-1-oxobutan-2-yl]amino]-1-oxobutan-2-yl]-6-methyloctanamide sulfuric acid Polymers OS(O)(=O)=O.CC(C)CCCCC(=O)N[C@@H](CCN)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CCN)C(=O)N[C@H]1CCNC(=O)[C@@H](NC(=O)[C@H](CCN)NC(=O)[C@H](CCN)NC(=O)[C@H](CC(C)C)NC(=O)[C@@H](Cc2ccccc2)NC(=O)[C@@H](CCN)NC1=O)[C@@H](C)O.CC[C@H](C)CCCCC(=O)N[C@@H](CCN)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CCN)C(=O)N[C@H]1CCNC(=O)[C@@H](NC(=O)[C@H](CCN)NC(=O)[C@H](CCN)NC(=O)[C@H](CC(C)C)NC(=O)[C@@H](Cc2ccccc2)NC(=O)[C@@H](CCN)NC1=O)[C@@H](C)O SBKRTALNRRAOJP-BWSIXKJUSA-N 0.000 description 4
- 102000004459 Nitroreductase Human genes 0.000 description 4
- 108010093965 Polymyxin B Proteins 0.000 description 4
- 108010059993 Vancomycin Proteins 0.000 description 4
- 108010028144 alpha-Glucosidases Proteins 0.000 description 4
- 125000004432 carbon atom Chemical group C* 0.000 description 4
- 230000002255 enzymatic effect Effects 0.000 description 4
- 239000007850 fluorescent dye Substances 0.000 description 4
- 229960002449 glycine Drugs 0.000 description 4
- 238000011081 inoculation Methods 0.000 description 4
- 108020001162 nitroreductase Proteins 0.000 description 4
- 229960003548 polymyxin b sulfate Drugs 0.000 description 4
- 239000007787 solid Substances 0.000 description 4
- 238000012360 testing method Methods 0.000 description 4
- 229960003165 vancomycin Drugs 0.000 description 4
- MYPYJXKWCTUITO-LYRMYLQWSA-N vancomycin Chemical compound O([C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1OC1=C2C=C3C=C1OC1=CC=C(C=C1Cl)[C@@H](O)[C@H](C(N[C@@H](CC(N)=O)C(=O)N[C@H]3C(=O)N[C@H]1C(=O)N[C@H](C(N[C@@H](C3=CC(O)=CC(O)=C3C=3C(O)=CC=C1C=3)C(O)=O)=O)[C@H](O)C1=CC=C(C(=C1)Cl)O2)=O)NC(=O)[C@@H](CC(C)C)NC)[C@H]1C[C@](C)(N)[C@H](O)[C@H](C)O1 MYPYJXKWCTUITO-LYRMYLQWSA-N 0.000 description 4
- MYPYJXKWCTUITO-UHFFFAOYSA-N vancomycin Natural products O1C(C(=C2)Cl)=CC=C2C(O)C(C(NC(C2=CC(O)=CC(O)=C2C=2C(O)=CC=C3C=2)C(O)=O)=O)NC(=O)C3NC(=O)C2NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(CC(C)C)NC)C(O)C(C=C3Cl)=CC=C3OC3=CC2=CC1=C3OC1OC(CO)C(O)C(O)C1OC1CC(C)(N)C(O)C(C)O1 MYPYJXKWCTUITO-UHFFFAOYSA-N 0.000 description 4
- ZPLCXHWYPWVJDL-UHFFFAOYSA-N 4-[(4-hydroxyphenyl)methyl]-1,3-oxazolidin-2-one Chemical compound C1=CC(O)=CC=C1CC1NC(=O)OC1 ZPLCXHWYPWVJDL-UHFFFAOYSA-N 0.000 description 3
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 3
- 229920002148 Gellan gum Polymers 0.000 description 3
- 241000589264 Legionella longbeachae Species 0.000 description 3
- 239000008280 blood Substances 0.000 description 3
- 210000004369 blood Anatomy 0.000 description 3
- 239000004927 clay Substances 0.000 description 3
- 238000003745 diagnosis Methods 0.000 description 3
- XJRPTMORGOIMMI-UHFFFAOYSA-N ethyl 2-amino-4-(trifluoromethyl)-1,3-thiazole-5-carboxylate Chemical compound CCOC(=O)C=1SC(N)=NC=1C(F)(F)F XJRPTMORGOIMMI-UHFFFAOYSA-N 0.000 description 3
- 230000004060 metabolic process Effects 0.000 description 3
- UPSFMJHZUCSEHU-JYGUBCOQSA-N n-[(2s,3r,4r,5s,6r)-2-[(2r,3s,4r,5r,6s)-5-acetamido-4-hydroxy-2-(hydroxymethyl)-6-(4-methyl-2-oxochromen-7-yl)oxyoxan-3-yl]oxy-4,5-dihydroxy-6-(hydroxymethyl)oxan-3-yl]acetamide Chemical compound CC(=O)N[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1O[C@H]1[C@H](O)[C@@H](NC(C)=O)[C@H](OC=2C=C3OC(=O)C=C(C)C3=CC=2)O[C@@H]1CO UPSFMJHZUCSEHU-JYGUBCOQSA-N 0.000 description 3
- 239000006152 selective media Substances 0.000 description 3
- 239000011550 stock solution Substances 0.000 description 3
- PUDWACYAXVIXIX-UHFFFAOYSA-N 2-(5-fluoro-2-nitrophenyl)-1,3-benzothiazole Chemical compound [O-][N+](=O)C1=CC=C(F)C=C1C1=NC2=CC=CC=C2S1 PUDWACYAXVIXIX-UHFFFAOYSA-N 0.000 description 2
- 238000002965 ELISA Methods 0.000 description 2
- 241000589239 Legionella erythra Species 0.000 description 2
- 241000589272 Legionella rubrilucens Species 0.000 description 2
- 208000019693 Lung disease Diseases 0.000 description 2
- 238000004378 air conditioning Methods 0.000 description 2
- RGCKGOZRHPZPFP-UHFFFAOYSA-N alizarin Chemical compound C1=CC=C2C(=O)C3=C(O)C(O)=CC=C3C(=O)C2=C1 RGCKGOZRHPZPFP-UHFFFAOYSA-N 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 239000000427 antigen Substances 0.000 description 2
- 239000012472 biological sample Substances 0.000 description 2
- 239000003086 colorant Substances 0.000 description 2
- 238000001816 cooling Methods 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 230000007613 environmental effect Effects 0.000 description 2
- 238000000605 extraction Methods 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 239000003349 gelling agent Substances 0.000 description 2
- 238000010166 immunofluorescence Methods 0.000 description 2
- 208000015181 infectious disease Diseases 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 230000002503 metabolic effect Effects 0.000 description 2
- 238000002156 mixing Methods 0.000 description 2
- 229960003255 natamycin Drugs 0.000 description 2
- NCXMLFZGDNKEPB-FFPOYIOWSA-N natamycin Chemical compound O[C@H]1[C@@H](N)[C@H](O)[C@@H](C)O[C@H]1O[C@H]1/C=C/C=C/C=C/C=C/C[C@@H](C)OC(=O)/C=C/[C@H]2O[C@@H]2C[C@H](O)C[C@](O)(C[C@H](O)[C@H]2C(O)=O)O[C@H]2C1 NCXMLFZGDNKEPB-FFPOYIOWSA-N 0.000 description 2
- 235000010298 natamycin Nutrition 0.000 description 2
- 239000004311 natamycin Substances 0.000 description 2
- 230000003287 optical effect Effects 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 239000002904 solvent Substances 0.000 description 2
- 241000894007 species Species 0.000 description 2
- 239000000725 suspension Substances 0.000 description 2
- PWKSKIMOESPYIA-UHFFFAOYSA-N 2-acetamido-3-sulfanylpropanoic acid Chemical compound CC(=O)NC(CS)C(O)=O PWKSKIMOESPYIA-UHFFFAOYSA-N 0.000 description 1
- GOLORTLGFDVFDW-UHFFFAOYSA-N 3-(1h-benzimidazol-2-yl)-7-(diethylamino)chromen-2-one Chemical compound C1=CC=C2NC(C3=CC4=CC=C(C=C4OC3=O)N(CC)CC)=NC2=C1 GOLORTLGFDVFDW-UHFFFAOYSA-N 0.000 description 1
- 229920000936 Agarose Polymers 0.000 description 1
- 101710099705 Anti-lipopolysaccharide factor Proteins 0.000 description 1
- COXVTLYNGOIATD-HVMBLDELSA-N CC1=C(C=CC(=C1)C1=CC(C)=C(C=C1)\N=N\C1=C(O)C2=C(N)C(=CC(=C2C=C1)S(O)(=O)=O)S(O)(=O)=O)\N=N\C1=CC=C2C(=CC(=C(N)C2=C1O)S(O)(=O)=O)S(O)(=O)=O Chemical compound CC1=C(C=CC(=C1)C1=CC(C)=C(C=C1)\N=N\C1=C(O)C2=C(N)C(=CC(=C2C=C1)S(O)(=O)=O)S(O)(=O)=O)\N=N\C1=CC=C2C(=CC(=C(N)C2=C1O)S(O)(=O)=O)S(O)(=O)=O COXVTLYNGOIATD-HVMBLDELSA-N 0.000 description 1
- 241000606161 Chlamydia Species 0.000 description 1
- 206010011224 Cough Diseases 0.000 description 1
- 101710088194 Dehydrogenase Proteins 0.000 description 1
- 206010012218 Delirium Diseases 0.000 description 1
- 206010012735 Diarrhoea Diseases 0.000 description 1
- 108090000371 Esterases Proteins 0.000 description 1
- 241000192125 Firmicutes Species 0.000 description 1
- 241000589565 Flavobacterium Species 0.000 description 1
- 208000018522 Gastrointestinal disease Diseases 0.000 description 1
- 108010010803 Gelatin Proteins 0.000 description 1
- 241000589276 Legionella jordanis Species 0.000 description 1
- 241000937819 Legionella pneumophila serogroup 1 Species 0.000 description 1
- 241000589246 Legionellaceae Species 0.000 description 1
- 241000204031 Mycoplasma Species 0.000 description 1
- 101710163270 Nuclease Proteins 0.000 description 1
- 108091005804 Peptidases Proteins 0.000 description 1
- 102000035195 Peptidases Human genes 0.000 description 1
- 208000005228 Pericardial Effusion Diseases 0.000 description 1
- 206010035718 Pneumonia legionella Diseases 0.000 description 1
- 206010036790 Productive cough Diseases 0.000 description 1
- 241000589516 Pseudomonas Species 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- XUIMIQQOPSSXEZ-UHFFFAOYSA-N Silicon Chemical compound [Si] XUIMIQQOPSSXEZ-UHFFFAOYSA-N 0.000 description 1
- 206010041349 Somnolence Diseases 0.000 description 1
- 102000019197 Superoxide Dismutase Human genes 0.000 description 1
- 108010012715 Superoxide dismutase Proteins 0.000 description 1
- GWEVSGVZZGPLCZ-UHFFFAOYSA-N Titan oxide Chemical compound O=[Ti]=O GWEVSGVZZGPLCZ-UHFFFAOYSA-N 0.000 description 1
- 206010047700 Vomiting Diseases 0.000 description 1
- 108091000039 acetoacetyl-CoA reductase Proteins 0.000 description 1
- 230000001154 acute effect Effects 0.000 description 1
- 239000000443 aerosol Substances 0.000 description 1
- HWXBTNAVRSUOJR-UHFFFAOYSA-N alpha-hydroxyglutaric acid Natural products OC(=O)C(O)CCC(O)=O HWXBTNAVRSUOJR-UHFFFAOYSA-N 0.000 description 1
- 229940024606 amino acid Drugs 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 239000003242 anti bacterial agent Substances 0.000 description 1
- 229940088710 antibiotic agent Drugs 0.000 description 1
- 102000036639 antigens Human genes 0.000 description 1
- 108091007433 antigens Proteins 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- KPGXRSRHYNQIFN-UHFFFAOYSA-N beta-keto-glutaric acid Natural products OC(=O)CCC(=O)C(O)=O KPGXRSRHYNQIFN-UHFFFAOYSA-N 0.000 description 1
- 238000001574 biopsy Methods 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 125000000484 butyl group Chemical group [H]C([*])([H])C([H])([H])C([H])([H])C([H])([H])[H] 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 235000014633 carbohydrates Nutrition 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 210000001175 cerebrospinal fluid Anatomy 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 230000037029 cross reaction Effects 0.000 description 1
- 238000012136 culture method Methods 0.000 description 1
- 230000014670 detection of bacterium Effects 0.000 description 1
- GUJOJGAPFQRJSV-UHFFFAOYSA-N dialuminum;dioxosilane;oxygen(2-);hydrate Chemical compound O.[O-2].[O-2].[O-2].[Al+3].[Al+3].O=[Si]=O.O=[Si]=O.O=[Si]=O.O=[Si]=O GUJOJGAPFQRJSV-UHFFFAOYSA-N 0.000 description 1
- 230000004069 differentiation Effects 0.000 description 1
- HNPSIPDUKPIQMN-UHFFFAOYSA-N dioxosilane;oxo(oxoalumanyloxy)alumane Chemical class O=[Si]=O.O=[Al]O[Al]=O HNPSIPDUKPIQMN-UHFFFAOYSA-N 0.000 description 1
- 239000003651 drinking water Substances 0.000 description 1
- 235000020188 drinking water Nutrition 0.000 description 1
- 239000000975 dye Substances 0.000 description 1
- 238000013399 early diagnosis Methods 0.000 description 1
- 229960003699 evans blue Drugs 0.000 description 1
- 210000003608 fece Anatomy 0.000 description 1
- 229940124307 fluoroquinolone Drugs 0.000 description 1
- 239000000499 gel Substances 0.000 description 1
- 239000008273 gelatin Substances 0.000 description 1
- 229920000159 gelatin Polymers 0.000 description 1
- 235000019322 gelatine Nutrition 0.000 description 1
- 235000011852 gelatine desserts Nutrition 0.000 description 1
- 229960003180 glutathione Drugs 0.000 description 1
- 239000003102 growth factor Substances 0.000 description 1
- 208000021760 high fever Diseases 0.000 description 1
- 229910052900 illite Inorganic materials 0.000 description 1
- 238000003317 immunochromatography Methods 0.000 description 1
- 238000010348 incorporation Methods 0.000 description 1
- 206010022000 influenza Diseases 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- NPFOYSMITVOQOS-UHFFFAOYSA-K iron(III) citrate Chemical compound [Fe+3].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NPFOYSMITVOQOS-UHFFFAOYSA-K 0.000 description 1
- 238000004020 luminiscence type Methods 0.000 description 1
- FDZZZRQASAIRJF-UHFFFAOYSA-M malachite green Chemical compound [Cl-].C1=CC(N(C)C)=CC=C1C(C=1C=CC=CC=1)=C1C=CC(=[N+](C)C)C=C1 FDZZZRQASAIRJF-UHFFFAOYSA-M 0.000 description 1
- 229940107698 malachite green Drugs 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 229910052901 montmorillonite Inorganic materials 0.000 description 1
- VBEGHXKAFSLLGE-UHFFFAOYSA-N n-phenylnitramide Chemical compound [O-][N+](=O)NC1=CC=CC=C1 VBEGHXKAFSLLGE-UHFFFAOYSA-N 0.000 description 1
- 230000000926 neurological effect Effects 0.000 description 1
- PGSADBUBUOPOJS-UHFFFAOYSA-N neutral red Chemical compound Cl.C1=C(C)C(N)=CC2=NC3=CC(N(C)C)=CC=C3N=C21 PGSADBUBUOPOJS-UHFFFAOYSA-N 0.000 description 1
- VGIBGUSAECPPNB-UHFFFAOYSA-L nonaaluminum;magnesium;tripotassium;1,3-dioxido-2,4,5-trioxa-1,3-disilabicyclo[1.1.1]pentane;iron(2+);oxygen(2-);fluoride;hydroxide Chemical compound [OH-].[O-2].[O-2].[O-2].[O-2].[O-2].[F-].[Mg+2].[Al+3].[Al+3].[Al+3].[Al+3].[Al+3].[Al+3].[Al+3].[Al+3].[Al+3].[K+].[K+].[K+].[Fe+2].O1[Si]2([O-])O[Si]1([O-])O2.O1[Si]2([O-])O[Si]1([O-])O2.O1[Si]2([O-])O[Si]1([O-])O2.O1[Si]2([O-])O[Si]1([O-])O2.O1[Si]2([O-])O[Si]1([O-])O2.O1[Si]2([O-])O[Si]1([O-])O2.O1[Si]2([O-])O[Si]1([O-])O2 VGIBGUSAECPPNB-UHFFFAOYSA-L 0.000 description 1
- 210000001331 nose Anatomy 0.000 description 1
- 239000002773 nucleotide Substances 0.000 description 1
- 125000003729 nucleotide group Chemical group 0.000 description 1
- 125000002347 octyl group Chemical group [H]C([*])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])[H] 0.000 description 1
- 239000003605 opacifier Substances 0.000 description 1
- 210000004912 pericardial fluid Anatomy 0.000 description 1
- 210000003800 pharynx Anatomy 0.000 description 1
- 238000011197 physicochemical method Methods 0.000 description 1
- 210000002381 plasma Anatomy 0.000 description 1
- 210000004910 pleural fluid Anatomy 0.000 description 1
- 208000008423 pleurisy Diseases 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 235000019833 protease Nutrition 0.000 description 1
- 230000002685 pulmonary effect Effects 0.000 description 1
- LISFMEBWQUVKPJ-UHFFFAOYSA-N quinolin-2-ol Chemical compound C1=CC=C2NC(=O)C=CC2=C1 LISFMEBWQUVKPJ-UHFFFAOYSA-N 0.000 description 1
- 208000023504 respiratory system disease Diseases 0.000 description 1
- 239000000741 silica gel Substances 0.000 description 1
- 229910002027 silica gel Inorganic materials 0.000 description 1
- 229910052710 silicon Inorganic materials 0.000 description 1
- 239000010703 silicon Substances 0.000 description 1
- 210000003491 skin Anatomy 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 125000004079 stearyl group Chemical group [H]C([*])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])[H] 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- OGIDPMRJRNCKJF-UHFFFAOYSA-N titanium oxide Inorganic materials [Ti]=O OGIDPMRJRNCKJF-UHFFFAOYSA-N 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 231100000167 toxic agent Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 210000002700 urine Anatomy 0.000 description 1
- 239000006200 vaporizer Substances 0.000 description 1
- 230000000007 visual effect Effects 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 230000008673 vomiting Effects 0.000 description 1
- 238000003809 water extraction Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
- C12Q1/045—Culture media therefor
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Analytical Chemistry (AREA)
- Toxicology (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| FR0857078A FR2937338B1 (fr) | 2008-10-17 | 2008-10-17 | Milieu reactionnel pour la detection et/ou l'identification de bacteries du genre legionella |
| FR0857078 | 2008-10-17 | ||
| PCT/FR2009/051961 WO2010043818A1 (fr) | 2008-10-17 | 2009-10-14 | Milieu reactionnel pour la detection et/ou l'identification de bacteries du genre legionella |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ES2536555T3 true ES2536555T3 (es) | 2015-05-26 |
Family
ID=40578309
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES09756026.2T Active ES2536555T3 (es) | 2008-10-17 | 2009-10-14 | Medio de reacción para la detección y/o la identificación de bacterias del género Legionela |
Country Status (8)
| Country | Link |
|---|---|
| US (1) | US8709746B2 (enExample) |
| EP (1) | EP2352839B1 (enExample) |
| JP (1) | JP5769174B2 (enExample) |
| CN (1) | CN102186986B (enExample) |
| ES (1) | ES2536555T3 (enExample) |
| FR (1) | FR2937338B1 (enExample) |
| PL (1) | PL2352839T3 (enExample) |
| WO (1) | WO2010043818A1 (enExample) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20150132585A (ko) * | 2013-03-22 | 2015-11-25 | 요우이치 미즈타 | 식물 조직의 배양용 배지의 제조 방법 및 식물 조직의 배양 방법 및 멸균 처리제, 살균 처리제, 및 식물 조직 배양용 배지 조성물 |
| US10179927B2 (en) | 2014-08-22 | 2019-01-15 | Nanyang Technological University | Electrochemical detection of microorganisms |
| JP2019201557A (ja) * | 2018-05-21 | 2019-11-28 | Jnc株式会社 | レジオネラ属細菌用培地の製造方法 |
| GB201813884D0 (en) | 2018-08-24 | 2018-10-10 | Sec Dep For Health | Culture media |
Family Cites Families (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| BE388559A (enExample) | 1932-05-18 | 1932-09-30 | Acec | |
| FR755371A (fr) | 1933-05-10 | 1933-11-23 | Paul Dubois Ets | Appareil de frappe à commande électrique |
| US5162229A (en) * | 1988-03-15 | 1992-11-10 | Akzo N.V. | Device and method for enhanced recovery and detection of microbial growth in the presence of antimicrobial substances |
| US5187066A (en) * | 1990-02-14 | 1993-02-16 | Syntex (U.S.A.) Inc. | Methods for detecting amphiphilic antigens |
| US5316906A (en) | 1991-08-23 | 1994-05-31 | Molecular Probes, Inc. | Enzymatic analysis using substrates that yield fluorescent precipitates |
| JPH05322897A (ja) * | 1992-05-21 | 1993-12-07 | Mitsubishi Yuka B C L:Kk | フィルターを用いる細菌の酵素免疫測定法 |
| US5314855A (en) * | 1992-11-09 | 1994-05-24 | Akzo N.V. | Adsorbent compositions and methods of manufacture |
| DE4419234A1 (de) * | 1994-06-01 | 1995-12-07 | Wacker Chemie Gmbh | Verfahren zur Silylierung von anorganischen Oxiden |
| FR2785620B1 (fr) | 1998-11-05 | 2003-02-07 | Bio Merieux | Detection de tous micro-organismes dans un echantillon |
| CU22844A1 (es) * | 2000-09-07 | 2004-02-20 | Ct Nac Biopreparados | Medio de cultivo y método para la identificación de microorgnismos gram-negativos |
| FR2845097B1 (fr) * | 2002-10-01 | 2006-06-16 | Metis Biotechnologies | Procede de detection et de comptage de microorganismes dans un echantillon |
| EP1869469B1 (en) * | 2005-03-17 | 2011-12-07 | Phigenics, LLC | Rapidly detecting and quantifying viable legionella |
| US9212384B2 (en) * | 2006-09-28 | 2015-12-15 | Tsumara & Co. | Microbial culture medium and microbial culture method using acid/activated clay |
-
2008
- 2008-10-17 FR FR0857078A patent/FR2937338B1/fr not_active Expired - Fee Related
-
2009
- 2009-10-14 JP JP2011531540A patent/JP5769174B2/ja not_active Expired - Fee Related
- 2009-10-14 PL PL09756026T patent/PL2352839T3/pl unknown
- 2009-10-14 ES ES09756026.2T patent/ES2536555T3/es active Active
- 2009-10-14 WO PCT/FR2009/051961 patent/WO2010043818A1/fr not_active Ceased
- 2009-10-14 CN CN200980140979.XA patent/CN102186986B/zh not_active Expired - Fee Related
- 2009-10-14 EP EP09756026.2A patent/EP2352839B1/fr active Active
- 2009-10-14 US US13/120,050 patent/US8709746B2/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| CN102186986B (zh) | 2014-05-07 |
| JP2012505648A (ja) | 2012-03-08 |
| FR2937338A1 (fr) | 2010-04-23 |
| EP2352839B1 (fr) | 2015-02-25 |
| EP2352839A1 (fr) | 2011-08-10 |
| WO2010043818A1 (fr) | 2010-04-22 |
| US20110171667A1 (en) | 2011-07-14 |
| CN102186986A (zh) | 2011-09-14 |
| US8709746B2 (en) | 2014-04-29 |
| JP5769174B2 (ja) | 2015-08-26 |
| PL2352839T3 (pl) | 2015-08-31 |
| FR2937338B1 (fr) | 2013-03-22 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| ES2274074T3 (es) | Medio de deteccion especifico de staphylococcus aureus y procedimientos de indentificacion y/o de recuento que usan dichos medios. | |
| JP5390568B2 (ja) | 微生物の検出方法 | |
| ES2261189T3 (es) | Medio cromogeno de deteccion de sataphylococcus aureus. | |
| AU657688B2 (en) | Novel and improved method for determination of E. coli in water | |
| US8741597B2 (en) | Reaction medium for methicillin-resistant Staphylococcus aureus | |
| BR112013016757B1 (pt) | Método para detectar a presença ou ausência de um micro-organismo alvo | |
| AU731884B2 (en) | Culture medium for detecting pathogenic bacteria of the genus listeria and method for identifying said bacteria | |
| ES2536555T3 (es) | Medio de reacción para la detección y/o la identificación de bacterias del género Legionela | |
| ES2449497T3 (es) | Medio de cultivo y de identificación específica de diferentes especies de Candida y procedimientos de análisis | |
| US5411867A (en) | Method for determination of E. coli in water | |
| ES2440782T3 (es) | Procedimiento para la identificación de, al menos, dos grupos de microorganismos | |
| ES2618076T3 (es) | Medio de reacción para las bacterias Staphylococcus aureus resistentes a la meticilina (MRSA) | |
| ES2428076T3 (es) | Procedimiento de identificación de bacterias del grupo Bacillus cereus | |
| ES2663235T3 (es) | Procedimiento de detección de bacterias productoras de carbapenemasas de tipo OXA-48 | |
| ES2275338T3 (es) | Nuevos substratos cromogenos para la deteccion de hidrolasas bacterianas. | |
| ES2377658T3 (es) | Medios de ensayo y procedimiento de identificación y diferenciación de bacterias | |
| ES2647451T3 (es) | Medio de reacción para las bacterias Vibrio | |
| ES2666132T3 (es) | Medio de detección de microorganismos que comprende al menos un alquil(tio)glucósido | |
| US8846336B2 (en) | Test mixtures for detecting the presence or absence of target microbes | |
| ES2653243T3 (es) | Medio de reacción para las bacterias Staphylococcus aureus resistentes a la meticilina (MRSA) | |
| CN101035907B (zh) | 用酯酶活性来检测无乳链球菌的方法 | |
| ES2645455T3 (es) | Medio cromogénico para detección e identificación de enterococos resistentes a vancomicina y procedimiento asociado | |
| JP7503384B2 (ja) | レジオネラ属菌鑑別用発色培地 | |
| JP5354564B2 (ja) | 大腸菌ｏ２６およびｏ１５７の同時検出培地ならびにその検出法 | |
| JP6124912B2 (ja) | 病原性エルシニア・エンテロコリチカ細菌を検出するための培地および方法 |