ES2530466T3 - Recuperación mejorada de ácidos nucleicos de partículas magnéticas de vidrio - Google Patents
Recuperación mejorada de ácidos nucleicos de partículas magnéticas de vidrio Download PDFInfo
- Publication number
- ES2530466T3 ES2530466T3 ES11701607T ES11701607T ES2530466T3 ES 2530466 T3 ES2530466 T3 ES 2530466T3 ES 11701607 T ES11701607 T ES 11701607T ES 11701607 T ES11701607 T ES 11701607T ES 2530466 T3 ES2530466 T3 ES 2530466T3
- Authority
- ES
- Spain
- Prior art keywords
- nucleic acids
- solid phase
- magnetic particles
- sample solution
- enhanced recovery
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
- C12N15/1013—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Biomedical Technology (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Plant Pathology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Immunology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
- Solid-Sorbent Or Filter-Aiding Compositions (AREA)
- Indole Compounds (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US12/684,762 US8420801B2 (en) | 2010-01-08 | 2010-01-08 | Recovery of nucleic acids from magnetic glass particles |
| PCT/EP2011/000013 WO2011083076A1 (en) | 2010-01-08 | 2011-01-05 | Improved recovery of nucleic acids from magnetic glass particles |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ES2530466T3 true ES2530466T3 (es) | 2015-03-02 |
Family
ID=43639930
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES11701607T Active ES2530466T3 (es) | 2010-01-08 | 2011-01-05 | Recuperación mejorada de ácidos nucleicos de partículas magnéticas de vidrio |
Country Status (7)
| Country | Link |
|---|---|
| US (1) | US8420801B2 (enExample) |
| EP (1) | EP2521779B1 (enExample) |
| JP (1) | JP5715158B2 (enExample) |
| CN (1) | CN102834518B (enExample) |
| CA (1) | CA2786644C (enExample) |
| ES (1) | ES2530466T3 (enExample) |
| WO (1) | WO2011083076A1 (enExample) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20150054844A (ko) * | 2012-09-19 | 2015-05-20 | 베크만 컬터, 인코포레이티드 | 핵산 추출에서의 2가 이온, 프로테아제, 계면활성제, 및 낮은 pH의 사용 |
| US10319502B2 (en) | 2014-10-23 | 2019-06-11 | Corning Incorporated | Polymer-encapsulated magnetic nanoparticles |
| US20200032241A1 (en) * | 2016-09-30 | 2020-01-30 | Eiken Kagaku Kabushiki Kaisha | Nucleic acid extraction method and kit using same |
| CN111278844B (zh) * | 2017-09-13 | 2023-12-19 | 贝克顿·迪金森公司 | 用于使用氧化铁颗粒提取核酸的方法和组合物 |
| CN107955810A (zh) * | 2017-12-25 | 2018-04-24 | 宁波卡尔新材料科技有限公司 | 一种新型动物肝脏核酸提取试剂盒及提取方法 |
| WO2020005584A1 (en) * | 2018-06-28 | 2020-01-02 | Gen-Probe Incorporated | Sample preparation method and system |
Family Cites Families (20)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5185243A (en) * | 1988-08-25 | 1993-02-09 | Syntex (U.S.A.) Inc. | Method for detection of specific nucleic acid sequences |
| US5573907A (en) * | 1990-01-26 | 1996-11-12 | Abbott Laboratories | Detecting and amplifying target nucleic acids using exonucleolytic activity |
| WO1995021271A1 (en) * | 1994-02-07 | 1995-08-10 | Molecular Tool, Inc. | Ligase/polymerase-mediated genetic bit analysistm of single nucleotide polymorphisms and its use in genetic analysis |
| DE19520398B4 (de) * | 1995-06-08 | 2009-04-16 | Roche Diagnostics Gmbh | Magnetisches Pigment |
| EP1154443A1 (en) | 2000-05-12 | 2001-11-14 | Boehringer Mannheim Gmbh | Magnetic glass particles, method for their preparation and uses thereof |
| WO1999003883A1 (en) * | 1997-07-18 | 1999-01-28 | Brigham And Women's Hospital | Compositions and methods based upon the tuberous sclerosis-1 (tsc1) gene and gene product |
| WO1999058644A1 (en) | 1998-05-14 | 1999-11-18 | University Of Hawaii | Compositions and methods for genetic transformation of pineapple |
| WO1999058664A1 (en) * | 1998-05-14 | 1999-11-18 | Whitehead Institute For Biomedical Research | Solid phase technique for selectively isolating nucleic acids |
| EP1204741B1 (en) * | 1999-08-20 | 2004-10-20 | Promega Corporation | Simultaneous isolation and quantitation of dna |
| US6548256B2 (en) | 2000-07-14 | 2003-04-15 | Eppendorf 5 Prime, Inc. | DNA isolation method and kit |
| EP1466018B2 (en) * | 2002-01-08 | 2015-08-12 | Roche Diagnostics GmbH | Use of silica material in an amplification reaction |
| GB0217963D0 (en) | 2002-08-02 | 2002-09-11 | Cyclops Genome Sciences Ltd | Purification of nucleic acids |
| EP1560926B2 (de) | 2002-11-08 | 2013-08-21 | STRATEC Molecular GmbH | Neuartige pufferformulierungen zur isolierung, reinigung und rückgewinnung lang- und kurzkettiger nukleinsäuren |
| EP1567671A1 (en) * | 2002-12-04 | 2005-08-31 | PamGene B.V. | Method for probe hybridisation of immobilized genomic dna |
| CN1970752A (zh) * | 2006-11-29 | 2007-05-30 | 吉林大学 | 用磁性纳米复合材料回收dna的方法及试剂盒 |
| WO2008119488A1 (en) | 2007-03-30 | 2008-10-09 | Roche Diagnostics Gmbh | Alkyl amines improve detection of components of formaldehyde-fixed biological samples |
| CA2629589C (en) | 2007-04-20 | 2016-03-29 | F.Hoffmann-La Roche Ag | Isolation and purification of nucleic acid molecules with a solid phase |
| JP5292722B2 (ja) * | 2007-05-09 | 2013-09-18 | 凸版印刷株式会社 | 未精製血液をサンプルとする核酸増幅法 |
| EP2157181A1 (de) | 2008-08-13 | 2010-02-24 | AGOWA Gesellschaft für molekularbiologische Technologie mbH | Verfahren zur Isolation von Nukleinsäuren und Testkit |
| AT508355B1 (de) | 2009-06-29 | 2011-01-15 | Trumpf Maschinen Austria Gmbh | Verfahren und vorrichtung zum biegen eines werkstücks |
-
2010
- 2010-01-08 US US12/684,762 patent/US8420801B2/en active Active
-
2011
- 2011-01-05 JP JP2012547490A patent/JP5715158B2/ja active Active
- 2011-01-05 EP EP11701607.1A patent/EP2521779B1/en active Active
- 2011-01-05 ES ES11701607T patent/ES2530466T3/es active Active
- 2011-01-05 WO PCT/EP2011/000013 patent/WO2011083076A1/en not_active Ceased
- 2011-01-05 CA CA2786644A patent/CA2786644C/en active Active
- 2011-01-05 CN CN201180005525.9A patent/CN102834518B/zh active Active
Also Published As
| Publication number | Publication date |
|---|---|
| US8420801B2 (en) | 2013-04-16 |
| JP5715158B2 (ja) | 2015-05-07 |
| CN102834518A (zh) | 2012-12-19 |
| WO2011083076A1 (en) | 2011-07-14 |
| JP2013516185A (ja) | 2013-05-13 |
| WO2011083076A8 (en) | 2014-08-07 |
| US20110172408A1 (en) | 2011-07-14 |
| CA2786644A1 (en) | 2011-07-14 |
| CA2786644C (en) | 2015-08-11 |
| EP2521779A1 (en) | 2012-11-14 |
| CN102834518B (zh) | 2015-04-01 |
| EP2521779B1 (en) | 2014-12-10 |
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