ES2530466T3 - Recuperación mejorada de ácidos nucleicos de partículas magnéticas de vidrio - Google Patents

Recuperación mejorada de ácidos nucleicos de partículas magnéticas de vidrio Download PDF

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Publication number
ES2530466T3
ES2530466T3 ES11701607T ES11701607T ES2530466T3 ES 2530466 T3 ES2530466 T3 ES 2530466T3 ES 11701607 T ES11701607 T ES 11701607T ES 11701607 T ES11701607 T ES 11701607T ES 2530466 T3 ES2530466 T3 ES 2530466T3
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Prior art keywords
nucleic acids
solid phase
magnetic particles
sample solution
enhanced recovery
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ES11701607T
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English (en)
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Jenny Johnson
Erich Kyger
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F Hoffmann La Roche AG
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F Hoffmann La Roche AG
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/1013—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
  • Indole Compounds (AREA)

Abstract

Un método para separar los ácidos nucleicos a partir de una solución de muestra que contiene ácidos nucleicos, que comprende a. poner en contacto la solución de la muestra con una fase sólida capaz de unirse a ácidos nucleicos y una mezcla de unión que comprende un compuesto de etileno-amina; b. incubar la solución de muestra que contiene la fase sólida bajo las condiciones en las que los ácidos nucleicos puede unirse a la fase sólida; y c. separar la fase sólida de la solución, en el que dicha fase sólida capaz de unirse a ácidos nucleicos comprende partículas que tienen un núcleo magnético y una capa exterior que contiene sílice y dicho compuesto de etileno-amina que tiene una fórmula general NH2-CH2-CH2-(NHCH2-CH2)n-NH2, en el que n es 0 - 10 o más.
ES11701607T 2010-01-08 2011-01-05 Recuperación mejorada de ácidos nucleicos de partículas magnéticas de vidrio Active ES2530466T3 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US12/684,762 US8420801B2 (en) 2010-01-08 2010-01-08 Recovery of nucleic acids from magnetic glass particles
PCT/EP2011/000013 WO2011083076A1 (en) 2010-01-08 2011-01-05 Improved recovery of nucleic acids from magnetic glass particles

Publications (1)

Publication Number Publication Date
ES2530466T3 true ES2530466T3 (es) 2015-03-02

Family

ID=43639930

Family Applications (1)

Application Number Title Priority Date Filing Date
ES11701607T Active ES2530466T3 (es) 2010-01-08 2011-01-05 Recuperación mejorada de ácidos nucleicos de partículas magnéticas de vidrio

Country Status (7)

Country Link
US (1) US8420801B2 (es)
EP (1) EP2521779B1 (es)
JP (1) JP5715158B2 (es)
CN (1) CN102834518B (es)
CA (1) CA2786644C (es)
ES (1) ES2530466T3 (es)
WO (1) WO2011083076A1 (es)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2015528313A (ja) * 2012-09-19 2015-09-28 ベックマン コールター， インコーポレイテッド 核酸の抽出における二価イオン、プロテアーゼ、洗剤、および低いｐｈの使用
WO2016065218A1 (en) 2014-10-23 2016-04-28 Corning Incorporated Polymer-encapsulated magnetic nanoparticles
WO2018061877A1 (ja) * 2016-09-30 2018-04-05 栄研化学株式会社 核酸を抽出する方法及びそれに用いるキット
WO2019055331A1 (en) 2017-09-13 2019-03-21 Becton, Dickinson And Company METHODS AND COMPOSITIONS FOR EXTRACTING NUCLEIC ACIDS USING FERRIQUE OXIDE PARTICLES
CN107955810A (zh) * 2017-12-25 2018-04-24 宁波卡尔新材料科技有限公司 一种新型动物肝脏核酸提取试剂盒及提取方法
EP3814496B1 (en) 2018-06-28 2023-11-22 Gen-Probe Incorporated Sample preparation method and system

Family Cites Families (20)

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US5185243A (en) * 1988-08-25 1993-02-09 Syntex (U.S.A.) Inc. Method for detection of specific nucleic acid sequences
US5573907A (en) * 1990-01-26 1996-11-12 Abbott Laboratories Detecting and amplifying target nucleic acids using exonucleolytic activity
AU694187B2 (en) * 1994-02-07 1998-07-16 Beckman Coulter, Inc. Ligase/polymerase-mediated genetic bit analysis TM of single nucleotide polymorphisms and its use in genetic analysis
EP1154443A1 (en) 2000-05-12 2001-11-14 Boehringer Mannheim Gmbh Magnetic glass particles, method for their preparation and uses thereof
DE19520398B4 (de) 1995-06-08 2009-04-16 Roche Diagnostics Gmbh Magnetisches Pigment
WO1999003883A1 (en) * 1997-07-18 1999-01-28 Brigham And Women's Hospital Compositions and methods based upon the tuberous sclerosis-1 (tsc1) gene and gene product
WO1999058664A1 (en) * 1998-05-14 1999-11-18 Whitehead Institute For Biomedical Research Solid phase technique for selectively isolating nucleic acids
AU3902799A (en) 1998-05-14 1999-11-29 University Of Hawaii Compositions and methods for genetic transformation of pineapple
AU780319B2 (en) * 1999-08-20 2005-03-17 Promega Corporation Simultaneous isolation and quantitation of DNA
US6548256B2 (en) 2000-07-14 2003-04-15 Eppendorf 5 Prime, Inc. DNA isolation method and kit
JP4148900B2 (ja) * 2002-01-08 2008-09-10 エフ．ホフマン−ラ ロシュ アーゲー 増幅反応におけるシリカ物質の使用
GB0217963D0 (en) * 2002-08-02 2002-09-11 Cyclops Genome Sciences Ltd Purification of nucleic acids
AU2003291931A1 (en) * 2002-11-08 2004-06-07 Invitek Gesellschaft Fur Biotechnik And Biodesign Mbh Novel buffer formulations for isolating, purifying and recovering long-chain and short-chain nucleic acids
EP1567671A1 (en) * 2002-12-04 2005-08-31 PamGene B.V. Method for probe hybridisation of immobilized genomic dna
CN1970752A (zh) * 2006-11-29 2007-05-30 吉林大学 用磁性纳米复合材料回收dna的方法及试剂盒
JP2010522544A (ja) 2007-03-30 2010-07-08 エフ．ホフマン−ラ ロシュ アーゲー ホルムアルデヒド−固定生物学的試料の成分のアルキルアミン改良検出
CA2629589C (en) * 2007-04-20 2016-03-29 F.Hoffmann-La Roche Ag Isolation and purification of nucleic acid molecules with a solid phase
JP5292722B2 (ja) * 2007-05-09 2013-09-18 凸版印刷株式会社 未精製血液をサンプルとする核酸増幅法
EP2157181A1 (de) 2008-08-13 2010-02-24 AGOWA Gesellschaft für molekularbiologische Technologie mbH Verfahren zur Isolation von Nukleinsäuren und Testkit
AT508355B1 (de) 2009-06-29 2011-01-15 Trumpf Maschinen Austria Gmbh Verfahren und vorrichtung zum biegen eines werkstücks

Also Published As

Publication number Publication date
CA2786644A1 (en) 2011-07-14
EP2521779B1 (en) 2014-12-10
CA2786644C (en) 2015-08-11
EP2521779A1 (en) 2012-11-14
CN102834518A (zh) 2012-12-19
WO2011083076A1 (en) 2011-07-14
WO2011083076A8 (en) 2014-08-07
JP2013516185A (ja) 2013-05-13
US20110172408A1 (en) 2011-07-14
JP5715158B2 (ja) 2015-05-07
US8420801B2 (en) 2013-04-16
CN102834518B (zh) 2015-04-01

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