CN102834518B - 改进的从磁性玻璃颗粒中的核酸回收 - Google Patents

改进的从磁性玻璃颗粒中的核酸回收 Download PDF

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Publication number
CN102834518B
CN102834518B CN201180005525.9A CN201180005525A CN102834518B CN 102834518 B CN102834518 B CN 102834518B CN 201180005525 A CN201180005525 A CN 201180005525A CN 102834518 B CN102834518 B CN 102834518B
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nucleic acid
solid phase
solution
nucleic acids
sample
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CN102834518A (zh
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J.A.约翰逊
E.凯格
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F Hoffmann La Roche AG
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F Hoffmann La Roche AG
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/1013—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
  • Indole Compounds (AREA)
CN201180005525.9A 2010-01-08 2011-01-05 改进的从磁性玻璃颗粒中的核酸回收 Active CN102834518B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US12/684,762 US8420801B2 (en) 2010-01-08 2010-01-08 Recovery of nucleic acids from magnetic glass particles
US12/684,762 2010-01-08
PCT/EP2011/000013 WO2011083076A1 (en) 2010-01-08 2011-01-05 Improved recovery of nucleic acids from magnetic glass particles

Publications (2)

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CN102834518A CN102834518A (zh) 2012-12-19
CN102834518B true CN102834518B (zh) 2015-04-01

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Country Link
US (1) US8420801B2 (enExample)
EP (1) EP2521779B1 (enExample)
JP (1) JP5715158B2 (enExample)
CN (1) CN102834518B (enExample)
CA (1) CA2786644C (enExample)
ES (1) ES2530466T3 (enExample)
WO (1) WO2011083076A1 (enExample)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20150054844A (ko) * 2012-09-19 2015-05-20 베크만 컬터, 인코포레이티드 핵산 추출에서의 2가 이온, 프로테아제, 계면활성제, 및 낮은 pH의 사용
US10319502B2 (en) 2014-10-23 2019-06-11 Corning Incorporated Polymer-encapsulated magnetic nanoparticles
US20200032241A1 (en) * 2016-09-30 2020-01-30 Eiken Kagaku Kabushiki Kaisha Nucleic acid extraction method and kit using same
CN111278844B (zh) * 2017-09-13 2023-12-19 贝克顿·迪金森公司 用于使用氧化铁颗粒提取核酸的方法和组合物
CN107955810A (zh) * 2017-12-25 2018-04-24 宁波卡尔新材料科技有限公司 一种新型动物肝脏核酸提取试剂盒及提取方法
WO2020005584A1 (en) * 2018-06-28 2020-01-02 Gen-Probe Incorporated Sample preparation method and system

Citations (8)

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CN1192217A (zh) * 1995-06-08 1998-09-02 曼海姆泊灵格股份公司 磁性色素
WO1999058664A1 (en) * 1998-05-14 1999-11-18 Whitehead Institute For Biomedical Research Solid phase technique for selectively isolating nucleic acids
CN1370230A (zh) * 1999-08-20 2002-09-18 普罗梅加公司 Dna的同时分离和定量
WO2004013155A2 (en) * 2002-08-02 2004-02-12 Cyclops Genome Sciences Limited Stabilisation of nucleic acids
WO2004042058A2 (de) * 2002-11-08 2004-05-21 InViTek Gesellschaft für Biotechnik & Biodesign mbH Neuartige pufferformulierungen zur isolierung, reinigung und rückgewinnung lang- und kurzkettiger nukleinsäuren
CN1970752A (zh) * 2006-11-29 2007-05-30 吉林大学 用磁性纳米复合材料回收dna的方法及试剂盒
WO2008119488A1 (en) * 2007-03-30 2008-10-09 Roche Diagnostics Gmbh Alkyl amines improve detection of components of formaldehyde-fixed biological samples
CN101289661A (zh) * 2007-04-20 2008-10-22 霍夫曼-拉罗奇有限公司 用固相分离和纯化核酸分子

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US5185243A (en) * 1988-08-25 1993-02-09 Syntex (U.S.A.) Inc. Method for detection of specific nucleic acid sequences
US5573907A (en) * 1990-01-26 1996-11-12 Abbott Laboratories Detecting and amplifying target nucleic acids using exonucleolytic activity
WO1995021271A1 (en) * 1994-02-07 1995-08-10 Molecular Tool, Inc. Ligase/polymerase-mediated genetic bit analysistm of single nucleotide polymorphisms and its use in genetic analysis
EP1154443A1 (en) 2000-05-12 2001-11-14 Boehringer Mannheim Gmbh Magnetic glass particles, method for their preparation and uses thereof
WO1999003883A1 (en) * 1997-07-18 1999-01-28 Brigham And Women's Hospital Compositions and methods based upon the tuberous sclerosis-1 (tsc1) gene and gene product
WO1999058644A1 (en) 1998-05-14 1999-11-18 University Of Hawaii Compositions and methods for genetic transformation of pineapple
US6548256B2 (en) 2000-07-14 2003-04-15 Eppendorf 5 Prime, Inc. DNA isolation method and kit
EP1466018B2 (en) * 2002-01-08 2015-08-12 Roche Diagnostics GmbH Use of silica material in an amplification reaction
EP1567671A1 (en) * 2002-12-04 2005-08-31 PamGene B.V. Method for probe hybridisation of immobilized genomic dna
JP5292722B2 (ja) * 2007-05-09 2013-09-18 凸版印刷株式会社 未精製血液をサンプルとする核酸増幅法
EP2157181A1 (de) 2008-08-13 2010-02-24 AGOWA Gesellschaft für molekularbiologische Technologie mbH Verfahren zur Isolation von Nukleinsäuren und Testkit
AT508355B1 (de) 2009-06-29 2011-01-15 Trumpf Maschinen Austria Gmbh Verfahren und vorrichtung zum biegen eines werkstücks

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Publication number Priority date Publication date Assignee Title
CN1192217A (zh) * 1995-06-08 1998-09-02 曼海姆泊灵格股份公司 磁性色素
WO1999058664A1 (en) * 1998-05-14 1999-11-18 Whitehead Institute For Biomedical Research Solid phase technique for selectively isolating nucleic acids
US20060003357A1 (en) * 1998-05-14 2006-01-05 Whitehead Institute For Biomedical Research Solid phase technique for selectively isolating nucleic acids
CN1370230A (zh) * 1999-08-20 2002-09-18 普罗梅加公司 Dna的同时分离和定量
WO2004013155A2 (en) * 2002-08-02 2004-02-12 Cyclops Genome Sciences Limited Stabilisation of nucleic acids
WO2004042058A2 (de) * 2002-11-08 2004-05-21 InViTek Gesellschaft für Biotechnik & Biodesign mbH Neuartige pufferformulierungen zur isolierung, reinigung und rückgewinnung lang- und kurzkettiger nukleinsäuren
CN1970752A (zh) * 2006-11-29 2007-05-30 吉林大学 用磁性纳米复合材料回收dna的方法及试剂盒
WO2008119488A1 (en) * 2007-03-30 2008-10-09 Roche Diagnostics Gmbh Alkyl amines improve detection of components of formaldehyde-fixed biological samples
CN101289661A (zh) * 2007-04-20 2008-10-22 霍夫曼-拉罗奇有限公司 用固相分离和纯化核酸分子

Non-Patent Citations (3)

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氧化铁磁性纳米粒子的表面修饰及应用;饶通德;《化工文摘》;20091231;59-62 *
磁性二氧化硅微球的表面修饰及其在植物基因组核酸纯化中的应用;张志超 等;《分析化学》;20070131;第35卷(第1期);31-36 *
铁基纳米粒子的制备及其在生物医学中的应用;高倩;《功能材料》;20081231;第39卷(第2期);181-185 *

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Publication number Publication date
US8420801B2 (en) 2013-04-16
JP5715158B2 (ja) 2015-05-07
CN102834518A (zh) 2012-12-19
ES2530466T3 (es) 2015-03-02
WO2011083076A1 (en) 2011-07-14
JP2013516185A (ja) 2013-05-13
WO2011083076A8 (en) 2014-08-07
US20110172408A1 (en) 2011-07-14
CA2786644A1 (en) 2011-07-14
CA2786644C (en) 2015-08-11
EP2521779A1 (en) 2012-11-14
EP2521779B1 (en) 2014-12-10

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