CA2786644C - Improved recovery of nucleic acids from magnetic glass particles - Google Patents

Improved recovery of nucleic acids from magnetic glass particles Download PDF

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Publication number
CA2786644C
CA2786644C CA2786644A CA2786644A CA2786644C CA 2786644 C CA2786644 C CA 2786644C CA 2786644 A CA2786644 A CA 2786644A CA 2786644 A CA2786644 A CA 2786644A CA 2786644 C CA2786644 C CA 2786644C
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CA
Canada
Prior art keywords
nucleic acids
binding
solid phase
ethylene
sample
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CA2786644A
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English (en)
French (fr)
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CA2786644A1 (en
Inventor
Jenny A. Johnson
Erich Kyger
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
F Hoffmann La Roche AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by F Hoffmann La Roche AG filed Critical F Hoffmann La Roche AG
Publication of CA2786644A1 publication Critical patent/CA2786644A1/en
Application granted granted Critical
Publication of CA2786644C publication Critical patent/CA2786644C/en
Active legal-status Critical Current
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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/1013—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
  • Indole Compounds (AREA)
CA2786644A 2010-01-08 2011-01-05 Improved recovery of nucleic acids from magnetic glass particles Active CA2786644C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US12/684,762 US8420801B2 (en) 2010-01-08 2010-01-08 Recovery of nucleic acids from magnetic glass particles
US12/684,762 2010-01-08
PCT/EP2011/000013 WO2011083076A1 (en) 2010-01-08 2011-01-05 Improved recovery of nucleic acids from magnetic glass particles

Publications (2)

Publication Number Publication Date
CA2786644A1 CA2786644A1 (en) 2011-07-14
CA2786644C true CA2786644C (en) 2015-08-11

Family

ID=43639930

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2786644A Active CA2786644C (en) 2010-01-08 2011-01-05 Improved recovery of nucleic acids from magnetic glass particles

Country Status (7)

Country Link
US (1) US8420801B2 (enExample)
EP (1) EP2521779B1 (enExample)
JP (1) JP5715158B2 (enExample)
CN (1) CN102834518B (enExample)
CA (1) CA2786644C (enExample)
ES (1) ES2530466T3 (enExample)
WO (1) WO2011083076A1 (enExample)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IN2015DN02479A (enExample) * 2012-09-19 2015-09-11 Beckman Coulter Inc
WO2016065218A1 (en) 2014-10-23 2016-04-28 Corning Incorporated Polymer-encapsulated magnetic nanoparticles
JPWO2018061877A1 (ja) * 2016-09-30 2019-06-24 栄研化学株式会社 核酸を抽出する方法及びそれに用いるキット
US11674133B2 (en) 2017-09-13 2023-06-13 Becton, Dickinson And Company Methods and compositions for extracting nucleic acids using ferric oxide particles
CN107955810A (zh) * 2017-12-25 2018-04-24 宁波卡尔新材料科技有限公司 一种新型动物肝脏核酸提取试剂盒及提取方法
US12344884B2 (en) 2018-06-28 2025-07-01 Gen-Probe Incorporated Sample preparation method and system

Family Cites Families (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5185243A (en) * 1988-08-25 1993-02-09 Syntex (U.S.A.) Inc. Method for detection of specific nucleic acid sequences
US5573907A (en) * 1990-01-26 1996-11-12 Abbott Laboratories Detecting and amplifying target nucleic acids using exonucleolytic activity
WO1995021271A1 (en) * 1994-02-07 1995-08-10 Molecular Tool, Inc. Ligase/polymerase-mediated genetic bit analysistm of single nucleotide polymorphisms and its use in genetic analysis
DE19520398B4 (de) 1995-06-08 2009-04-16 Roche Diagnostics Gmbh Magnetisches Pigment
EP1154443A1 (en) 2000-05-12 2001-11-14 Boehringer Mannheim Gmbh Magnetic glass particles, method for their preparation and uses thereof
WO1999003883A1 (en) * 1997-07-18 1999-01-28 Brigham And Women's Hospital Compositions and methods based upon the tuberous sclerosis-1 (tsc1) gene and gene product
US6534262B1 (en) * 1998-05-14 2003-03-18 Whitehead Institute For Biomedical Research Solid phase technique for selectively isolating nucleic acids
AU3902799A (en) 1998-05-14 1999-11-29 University Of Hawaii Compositions and methods for genetic transformation of pineapple
WO2001014590A2 (en) * 1999-08-20 2001-03-01 Promega Corporation Simultaneous isolation and quantitation of dna
US6548256B2 (en) 2000-07-14 2003-04-15 Eppendorf 5 Prime, Inc. DNA isolation method and kit
ATE374837T1 (de) * 2002-01-08 2007-10-15 Hoffmann La Roche Verwendung eines silikamaterials bei der amplifikation
GB0217963D0 (en) 2002-08-02 2002-09-11 Cyclops Genome Sciences Ltd Purification of nucleic acids
EP1560926B2 (de) * 2002-11-08 2013-08-21 STRATEC Molecular GmbH Neuartige pufferformulierungen zur isolierung, reinigung und rückgewinnung lang- und kurzkettiger nukleinsäuren
CA2511829A1 (en) * 2002-12-04 2004-06-17 Pamgene Bv Method for hybridisation of immobilized genomic dna
CN1970752A (zh) * 2006-11-29 2007-05-30 吉林大学 用磁性纳米复合材料回收dna的方法及试剂盒
CN105441549A (zh) 2007-03-30 2016-03-30 霍夫曼-拉罗奇有限公司 烷基胺改进甲醛固定的生物样品组分的检测
CA2629589C (en) 2007-04-20 2016-03-29 F.Hoffmann-La Roche Ag Isolation and purification of nucleic acid molecules with a solid phase
JP5292722B2 (ja) * 2007-05-09 2013-09-18 凸版印刷株式会社 未精製血液をサンプルとする核酸増幅法
EP2157181A1 (de) 2008-08-13 2010-02-24 AGOWA Gesellschaft für molekularbiologische Technologie mbH Verfahren zur Isolation von Nukleinsäuren und Testkit
AT508355B1 (de) 2009-06-29 2011-01-15 Trumpf Maschinen Austria Gmbh Verfahren und vorrichtung zum biegen eines werkstücks

Also Published As

Publication number Publication date
CN102834518B (zh) 2015-04-01
JP5715158B2 (ja) 2015-05-07
EP2521779A1 (en) 2012-11-14
US8420801B2 (en) 2013-04-16
JP2013516185A (ja) 2013-05-13
WO2011083076A1 (en) 2011-07-14
ES2530466T3 (es) 2015-03-02
WO2011083076A8 (en) 2014-08-07
US20110172408A1 (en) 2011-07-14
CA2786644A1 (en) 2011-07-14
EP2521779B1 (en) 2014-12-10
CN102834518A (zh) 2012-12-19

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