ES2490968T3 - Procedimiento para la preparación de 1,3-propanodiol a partir de sacarosa - Google Patents

Procedimiento para la preparación de 1,3-propanodiol a partir de sacarosa Download PDF

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Publication number
ES2490968T3
ES2490968T3 ES11728895.1T ES11728895T ES2490968T3 ES 2490968 T3 ES2490968 T3 ES 2490968T3 ES 11728895 T ES11728895 T ES 11728895T ES 2490968 T3 ES2490968 T3 ES 2490968T3
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sucrose
propanediol
microorganism
enzyme
utilization system
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English (en)
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Philippe Soucaille
Cédric BOISART
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Metabolic Explorer SA
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Metabolic Explorer SA
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/18—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic polyhydric
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1096—Transferases (2.) transferring nitrogenous groups (2.6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01021—Aldehyde reductase (1.1.1.21), i.e. aldose-reductase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y401/00—Carbon-carbon lyases (4.1)
    • C12Y401/01—Carboxy-lyases (4.1.1)
    • C12Y401/01001—Pyruvate decarboxylase (4.1.1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y206/00—Transferases transferring nitrogenous groups (2.6)
    • C12Y206/01—Transaminases (2.6.1)
    • C12Y206/01052—Phosphoserine transaminase (2.6.1.52)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Microorganismo genéticamente modificado para la bioproducción de 1,3-propanodiol a partir de sacarosa, en el que el microorganismo comprende: - una ruta metabólica de dos etapas para la producción de 1,3-propanodiol, que comprende una primera etapa de descarboxilación de 4-hidroxi-2-cetobutirato con una enzima que tiene una actividad de 2-cetoácido descarboxilasa codificada por un gen endógeno, cuya expresión está potenciada o por un gen heterólogo, y una segunda etapa de reducción del 3-hidroxipropionaldehído obtenido con una enzima que tiene actividad de hidroxi aldehído reductasa, y - unos genes funcionales que codifican un sistema de utilización de sacarosa PTS y/o un sistema de utilización de sacarosa no PTS, que permiten al microorganismo utilizar sacarosa como la única fuente de carbono.

Description

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Claims (1)

  1. imagen1
ES11728895.1T 2010-07-05 2011-07-05 Procedimiento para la preparación de 1,3-propanodiol a partir de sacarosa Active ES2490968T3 (es)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US361455P 2002-03-04
US36145510P 2010-07-05 2010-07-05
EP10305729 2010-07-05
EP10305729 2010-07-05
PCT/EP2011/061285 WO2012004247A1 (en) 2010-07-05 2011-07-05 Method for the preparation of 1,3-propanediol from sucrose

Publications (1)

Publication Number Publication Date
ES2490968T3 true ES2490968T3 (es) 2014-09-04

Family

ID=42712776

Family Applications (1)

Application Number Title Priority Date Filing Date
ES11728895.1T Active ES2490968T3 (es) 2010-07-05 2011-07-05 Procedimiento para la preparación de 1,3-propanodiol a partir de sacarosa

Country Status (12)

Country Link
US (1) US8900838B2 (es)
EP (1) EP2591091B1 (es)
JP (1) JP5420798B2 (es)
KR (1) KR101794384B1 (es)
CN (1) CN103097514B (es)
AR (1) AR082114A1 (es)
BR (1) BR112013000237B1 (es)
CA (1) CA2803070C (es)
ES (1) ES2490968T3 (es)
PT (1) PT2591091E (es)
TW (1) TWI500768B (es)
WO (1) WO2012004247A1 (es)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2014009432A2 (en) 2012-07-11 2014-01-16 Institut National Des Sciences Appliquées A microorganism modified for the production of 1,3-propanediol
EP2872640B1 (en) * 2012-07-11 2020-03-25 Adisseo France S.A.S. Method for the preparation of 2,4-dihydroxybutyrate
WO2016050959A2 (en) * 2014-10-03 2016-04-07 Metabolic Explorer Mutant glutamate dehydrogenase for the conversion of homoserine into 4-hydroxy-2-ketobutyrate
EP3050970B1 (en) 2015-01-28 2019-09-18 Metabolic Explorer Modified microorganism for optimized production of 1,4-butanediol
CN107690482B (zh) 2015-04-07 2022-11-29 代谢探索者公司 用于2,4-二羟基丁酸的优化生产的经修饰的微生物
KR102535038B1 (ko) 2015-09-08 2023-05-23 에스케이플래닛 주식회사 모바일 커머스 서비스 장치, 초음파 송수신 장치를 이용한 모바일 커머스 서비스 방법 및 컴퓨터 프로그램이 기록된 기록매체
RU2760290C1 (ru) 2016-07-08 2021-11-23 Метаболик Эксплорер Способ ферментативной продукции целевых молекул микроорганизмами, включающими гены, кодирующие фосфотрансферазную систему (фтс) сахаров
EP3470512A1 (en) 2017-10-10 2019-04-17 Metabolic Explorer Mutant phosphoserine aminotransferase for the conversion of homoserine into 4-hydroxy-2-ketobutyrate
CN108384796B (zh) * 2018-03-05 2021-11-26 北京化工大学 一种利用蔗糖生物合成d-葡萄糖二酸的方法
WO2020090017A1 (ja) * 2018-10-30 2020-05-07 Ｇｒｅｅｎ Ｅａｒｔｈ Ｉｎｓｔｉｔｕｔｅ 株式会社 １，３－プロパンジオールの製造方法
CN111705030B (zh) * 2020-07-07 2023-03-31 浙江工业大学 高产l-高丝氨酸的大肠杆菌基因工程菌、构建方法及菌株

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Also Published As

Publication number Publication date
JP2013529924A (ja) 2013-07-25
KR20130041166A (ko) 2013-04-24
TWI500768B (zh) 2015-09-21
EP2591091B1 (en) 2014-05-14
CN103097514B (zh) 2015-09-09
AR082114A1 (es) 2012-11-14
PT2591091E (pt) 2014-08-29
BR112013000237A2 (pt) 2016-05-17
BR112013000237B1 (pt) 2021-05-04
EP2591091A1 (en) 2013-05-15
TW201213545A (en) 2012-04-01
KR101794384B1 (ko) 2017-11-06
CA2803070A1 (en) 2012-01-12
US8900838B2 (en) 2014-12-02
CN103097514A (zh) 2013-05-08
WO2012004247A1 (en) 2012-01-12
US20130309737A1 (en) 2013-11-21
JP5420798B2 (ja) 2014-02-19
CA2803070C (en) 2019-10-08

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