ES2396151T3 - Potenciamiento de la producción de etanol microbiana - Google Patents
Potenciamiento de la producción de etanol microbiana Download PDFInfo
- Publication number
- ES2396151T3 ES2396151T3 ES07732122T ES07732122T ES2396151T3 ES 2396151 T3 ES2396151 T3 ES 2396151T3 ES 07732122 T ES07732122 T ES 07732122T ES 07732122 T ES07732122 T ES 07732122T ES 2396151 T3 ES2396151 T3 ES 2396151T3
- Authority
- ES
- Spain
- Prior art keywords
- dehydrogenase
- linked
- nad
- gene
- formate
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Links
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 title claims description 108
- 238000004519 manufacturing process Methods 0.000 title claims description 23
- 230000000813 microbial effect Effects 0.000 title description 5
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 100
- 241000894006 Bacteria Species 0.000 claims abstract description 49
- 108090000698 Formate Dehydrogenases Proteins 0.000 claims abstract description 39
- 108700023483 L-lactate dehydrogenases Proteins 0.000 claims abstract description 39
- 102000003855 L-lactate dehydrogenase Human genes 0.000 claims abstract description 26
- 241000193830 Bacillus <bacterium> Species 0.000 claims abstract description 25
- 230000000694 effects Effects 0.000 claims abstract description 19
- 108010008221 formate C-acetyltransferase Proteins 0.000 claims abstract description 14
- 101710088194 Dehydrogenase Proteins 0.000 claims description 46
- BDAGIHXWWSANSR-UHFFFAOYSA-M Formate Chemical compound [O-]C=O BDAGIHXWWSANSR-UHFFFAOYSA-M 0.000 claims description 43
- 235000000346 sugar Nutrition 0.000 claims description 29
- 239000002773 nucleotide Substances 0.000 claims description 22
- 125000003729 nucleotide group Chemical group 0.000 claims description 22
- 150000008163 sugars Chemical class 0.000 claims description 21
- 238000010276 construction Methods 0.000 claims description 19
- 230000010354 integration Effects 0.000 claims description 18
- 230000001105 regulatory effect Effects 0.000 claims description 16
- 230000004151 fermentation Effects 0.000 claims description 15
- 238000000855 fermentation Methods 0.000 claims description 15
- 238000005215 recombination Methods 0.000 claims description 8
- 230000006798 recombination Effects 0.000 claims description 8
- 230000001580 bacterial effect Effects 0.000 claims description 2
- 230000000415 inactivating effect Effects 0.000 claims description 2
- 239000013612 plasmid Substances 0.000 description 52
- 108020004414 DNA Proteins 0.000 description 45
- 244000005700 microbiome Species 0.000 description 40
- 101150086278 fdh gene Proteins 0.000 description 25
- 101150104734 ldh gene Proteins 0.000 description 18
- 238000003780 insertion Methods 0.000 description 16
- 230000037431 insertion Effects 0.000 description 16
- 230000014509 gene expression Effects 0.000 description 15
- 241000193385 Geobacillus stearothermophilus Species 0.000 description 14
- 210000004027 cell Anatomy 0.000 description 14
- 108020004705 Codon Proteins 0.000 description 13
- LCTONWCANYUPML-UHFFFAOYSA-M Pyruvate Chemical compound CC(=O)C([O-])=O LCTONWCANYUPML-UHFFFAOYSA-M 0.000 description 12
- 101100446293 Schizosaccharomyces pombe (strain 972 / ATCC 24843) fbh1 gene Proteins 0.000 description 11
- 239000000047 product Substances 0.000 description 9
- 241000588724 Escherichia coli Species 0.000 description 8
- 238000012217 deletion Methods 0.000 description 8
- 230000012010 growth Effects 0.000 description 8
- 229930027917 kanamycin Natural products 0.000 description 8
- 229960000318 kanamycin Drugs 0.000 description 8
- SBUJHOSQTJFQJX-NOAMYHISSA-N kanamycin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CN)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](N)[C@H](O)[C@@H](CO)O2)O)[C@H](N)C[C@@H]1N SBUJHOSQTJFQJX-NOAMYHISSA-N 0.000 description 8
- 229930182823 kanamycin A Natural products 0.000 description 8
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 7
- 102000004190 Enzymes Human genes 0.000 description 7
- 108090000790 Enzymes Proteins 0.000 description 7
- 238000010367 cloning Methods 0.000 description 7
- 230000037430 deletion Effects 0.000 description 7
- 230000037353 metabolic pathway Effects 0.000 description 7
- 230000037361 pathway Effects 0.000 description 7
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 6
- 239000012634 fragment Substances 0.000 description 6
- BOPGDPNILDQYTO-NNYOXOHSSA-N nicotinamide-adenine dinucleotide Chemical compound C1=CCC(C(=O)N)=CN1[C@H]1[C@H](O)[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OC[C@@H]2[C@H]([C@@H](O)[C@@H](O2)N2C3=NC=NC(N)=C3N=C2)O)O1 BOPGDPNILDQYTO-NNYOXOHSSA-N 0.000 description 6
- 108091026890 Coding region Proteins 0.000 description 5
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 5
- 238000004458 analytical method Methods 0.000 description 5
- 244000052616 bacterial pathogen Species 0.000 description 5
- 238000000034 method Methods 0.000 description 5
- 229930027945 nicotinamide-adenine dinucleotide Natural products 0.000 description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 5
- 239000002028 Biomass Substances 0.000 description 4
- JVTAAEKCZFNVCJ-UHFFFAOYSA-M Lactate Chemical compound CC(O)C([O-])=O JVTAAEKCZFNVCJ-UHFFFAOYSA-M 0.000 description 4
- 238000012408 PCR amplification Methods 0.000 description 4
- 241000193390 Parageobacillus thermoglucosidasius Species 0.000 description 4
- ZSLZBFCDCINBPY-ZSJPKINUSA-N acetyl-CoA Chemical compound O[C@@H]1[C@H](OP(O)(O)=O)[C@@H](COP(O)(=O)OP(O)(=O)OCC(C)(C)[C@@H](O)C(=O)NCCC(=O)NCCSC(=O)C)O[C@H]1N1C2=NC=NC(N)=C2N=C1 ZSLZBFCDCINBPY-ZSJPKINUSA-N 0.000 description 4
- 125000003275 alpha amino acid group Chemical group 0.000 description 4
- 230000034659 glycolysis Effects 0.000 description 4
- 108091008146 restriction endonucleases Proteins 0.000 description 4
- 239000013598 vector Substances 0.000 description 4
- 101150084229 ATXN1 gene Proteins 0.000 description 3
- 235000014469 Bacillus subtilis Nutrition 0.000 description 3
- 102000004163 DNA-directed RNA polymerases Human genes 0.000 description 3
- 108090000626 DNA-directed RNA polymerases Proteins 0.000 description 3
- 241000620209 Escherichia coli DH5[alpha] Species 0.000 description 3
- 108700039691 Genetic Promoter Regions Proteins 0.000 description 3
- 108700007698 Genetic Terminator Regions Proteins 0.000 description 3
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 3
- 229920002488 Hemicellulose Polymers 0.000 description 3
- 108091028043 Nucleic acid sequence Proteins 0.000 description 3
- 241000589774 Pseudomonas sp. Species 0.000 description 3
- 229920002678 cellulose Polymers 0.000 description 3
- 235000010980 cellulose Nutrition 0.000 description 3
- 238000001704 evaporation Methods 0.000 description 3
- 230000008020 evaporation Effects 0.000 description 3
- 238000000338 in vitro Methods 0.000 description 3
- 230000001939 inductive effect Effects 0.000 description 3
- 230000007935 neutral effect Effects 0.000 description 3
- -1 pentose sugars Chemical class 0.000 description 3
- 239000011780 sodium chloride Substances 0.000 description 3
- 201000003624 spinocerebellar ataxia type 1 Diseases 0.000 description 3
- 230000009466 transformation Effects 0.000 description 3
- 239000012137 tryptone Substances 0.000 description 3
- 229910001868 water Inorganic materials 0.000 description 3
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 description 2
- QTBSBXVTEAMEQO-UHFFFAOYSA-M Acetate Chemical compound CC([O-])=O QTBSBXVTEAMEQO-UHFFFAOYSA-M 0.000 description 2
- 229920001817 Agar Polymers 0.000 description 2
- 244000063299 Bacillus subtilis Species 0.000 description 2
- 108010005512 Cytosine 5-methyltransferase Proteins 0.000 description 2
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 2
- 108010043121 Green Fluorescent Proteins Proteins 0.000 description 2
- 102000004144 Green Fluorescent Proteins Human genes 0.000 description 2
- TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 description 2
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 2
- 108700026244 Open Reading Frames Proteins 0.000 description 2
- 108700005078 Synthetic Genes Proteins 0.000 description 2
- 239000008272 agar Substances 0.000 description 2
- 229940060587 alpha e Drugs 0.000 description 2
- 229960000723 ampicillin Drugs 0.000 description 2
- AVKUERGKIZMTKX-NJBDSQKTSA-N ampicillin Chemical compound C1([C@@H](N)C(=O)N[C@H]2[C@H]3SC([C@@H](N3C2=O)C(O)=O)(C)C)=CC=CC=C1 AVKUERGKIZMTKX-NJBDSQKTSA-N 0.000 description 2
- 230000003321 amplification Effects 0.000 description 2
- 230000036983 biotransformation Effects 0.000 description 2
- 239000000872 buffer Substances 0.000 description 2
- 229940041514 candida albicans extract Drugs 0.000 description 2
- 239000001913 cellulose Substances 0.000 description 2
- 230000034994 death Effects 0.000 description 2
- 230000007423 decrease Effects 0.000 description 2
- 239000008367 deionised water Substances 0.000 description 2
- 229910021641 deionized water Inorganic materials 0.000 description 2
- 239000013604 expression vector Substances 0.000 description 2
- 101150014582 fdhF gene Proteins 0.000 description 2
- 238000001914 filtration Methods 0.000 description 2
- 238000012224 gene deletion Methods 0.000 description 2
- 239000008103 glucose Substances 0.000 description 2
- 239000005090 green fluorescent protein Substances 0.000 description 2
- 101150066555 lacZ gene Proteins 0.000 description 2
- 239000012528 membrane Substances 0.000 description 2
- 230000002503 metabolic effect Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000003199 nucleic acid amplification method Methods 0.000 description 2
- 108020004707 nucleic acids Proteins 0.000 description 2
- 102000039446 nucleic acids Human genes 0.000 description 2
- 150000007523 nucleic acids Chemical class 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- KDYFGRWQOYBRFD-UHFFFAOYSA-L succinate(2-) Chemical compound [O-]C(=O)CCC([O-])=O KDYFGRWQOYBRFD-UHFFFAOYSA-L 0.000 description 2
- 238000013518 transcription Methods 0.000 description 2
- 230000035897 transcription Effects 0.000 description 2
- 239000012138 yeast extract Substances 0.000 description 2
- HDTRYLNUVZCQOY-UHFFFAOYSA-N α-D-glucopyranosyl-α-D-glucopyranoside Natural products OC1C(O)C(O)C(CO)OC1OC1C(O)C(O)C(O)C(CO)O1 HDTRYLNUVZCQOY-UHFFFAOYSA-N 0.000 description 1
- OWEGMIWEEQEYGQ-UHFFFAOYSA-N 100676-05-9 Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC2C(OC(O)C(O)C2O)CO)O1 OWEGMIWEEQEYGQ-UHFFFAOYSA-N 0.000 description 1
- JKMHFZQWWAIEOD-UHFFFAOYSA-N 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid Chemical compound OCC[NH+]1CCN(CCS([O-])(=O)=O)CC1 JKMHFZQWWAIEOD-UHFFFAOYSA-N 0.000 description 1
- 230000002407 ATP formation Effects 0.000 description 1
- 235000005744 Bacillus subtilis subsp subtilis Nutrition 0.000 description 1
- 241000948854 Bacillus subtilis subsp. subtilis Species 0.000 description 1
- 241000219310 Beta vulgaris subsp. vulgaris Species 0.000 description 1
- 244000025254 Cannabis sativa Species 0.000 description 1
- 108091035707 Consensus sequence Proteins 0.000 description 1
- 241000573484 Copsychus Species 0.000 description 1
- 102000012410 DNA Ligases Human genes 0.000 description 1
- 108010061982 DNA Ligases Proteins 0.000 description 1
- 108010069993 DNA modification methylase HaeII Proteins 0.000 description 1
- 108020005199 Dehydrogenases Proteins 0.000 description 1
- 101100080807 Drosophila melanogaster mt:ND2 gene Proteins 0.000 description 1
- 241000305071 Enterobacterales Species 0.000 description 1
- 244000068988 Glycine max Species 0.000 description 1
- 235000010469 Glycine max Nutrition 0.000 description 1
- 239000007995 HEPES buffer Substances 0.000 description 1
- 101150016680 MT-ND2 gene Proteins 0.000 description 1
- GUBGYTABKSRVRQ-PICCSMPSSA-N Maltose Natural products O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-PICCSMPSSA-N 0.000 description 1
- 102100028488 NADH-ubiquinone oxidoreductase chain 2 Human genes 0.000 description 1
- 101150102231 ND2 gene Proteins 0.000 description 1
- 238000010222 PCR analysis Methods 0.000 description 1
- 239000001888 Peptone Substances 0.000 description 1
- 108010080698 Peptones Proteins 0.000 description 1
- 240000000111 Saccharum officinarum Species 0.000 description 1
- 235000007201 Saccharum officinarum Nutrition 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
- 235000021536 Sugar beet Nutrition 0.000 description 1
- HDTRYLNUVZCQOY-WSWWMNSNSA-N Trehalose Natural products O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-WSWWMNSNSA-N 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 230000002378 acidificating effect Effects 0.000 description 1
- 239000002154 agricultural waste Substances 0.000 description 1
- HDTRYLNUVZCQOY-LIZSDCNHSA-N alpha,alpha-trehalose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-LIZSDCNHSA-N 0.000 description 1
- 230000006229 amino acid addition Effects 0.000 description 1
- 230000009604 anaerobic growth Effects 0.000 description 1
- 239000003242 anti bacterial agent Substances 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- 102000005936 beta-Galactosidase Human genes 0.000 description 1
- 108010005774 beta-Galactosidase Proteins 0.000 description 1
- GUBGYTABKSRVRQ-QUYVBRFLSA-N beta-maltose Chemical compound OC[C@H]1O[C@H](O[C@H]2[C@H](O)[C@@H](O)[C@H](O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@@H]1O GUBGYTABKSRVRQ-QUYVBRFLSA-N 0.000 description 1
- 230000003115 biocidal effect Effects 0.000 description 1
- 239000006227 byproduct Substances 0.000 description 1
- 230000003197 catalytic effect Effects 0.000 description 1
- 230000030833 cell death Effects 0.000 description 1
- 230000010261 cell growth Effects 0.000 description 1
- 235000013339 cereals Nutrition 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 210000000349 chromosome Anatomy 0.000 description 1
- 239000013599 cloning vector Substances 0.000 description 1
- 239000002361 compost Substances 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 description 1
- 229910000396 dipotassium phosphate Inorganic materials 0.000 description 1
- 235000019797 dipotassium phosphate Nutrition 0.000 description 1
- 238000004821 distillation Methods 0.000 description 1
- 230000027721 electron transport chain Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000013613 expression plasmid Substances 0.000 description 1
- 150000004675 formic acid derivatives Chemical class 0.000 description 1
- CNNRFBWVTKFGRE-UHFFFAOYSA-N formic acid;2-oxopropanoic acid Chemical compound OC=O.CC(=O)C(O)=O CNNRFBWVTKFGRE-UHFFFAOYSA-N 0.000 description 1
- 150000002402 hexoses Chemical class 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 230000002779 inactivation Effects 0.000 description 1
- 238000010348 incorporation Methods 0.000 description 1
- 230000003834 intracellular effect Effects 0.000 description 1
- 239000003446 ligand Substances 0.000 description 1
- 229910001629 magnesium chloride Inorganic materials 0.000 description 1
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 1
- 235000019341 magnesium sulphate Nutrition 0.000 description 1
- 239000003550 marker Substances 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000012269 metabolic engineering Methods 0.000 description 1
- 230000006609 metabolic stress Effects 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 230000035772 mutation Effects 0.000 description 1
- VIKNJXKGJWUCNN-XGXHKTLJSA-N norethisterone Chemical compound O=C1CC[C@@H]2[C@H]3CC[C@](C)([C@](CC4)(O)C#C)[C@@H]4[C@@H]3CCC2=C1 VIKNJXKGJWUCNN-XGXHKTLJSA-N 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 238000005457 optimization Methods 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 150000002972 pentoses Chemical class 0.000 description 1
- 235000019319 peptone Nutrition 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 229920006395 saturated elastomer Polymers 0.000 description 1
- 238000012163 sequencing technique Methods 0.000 description 1
- 230000010473 stable expression Effects 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- 238000013519 translation Methods 0.000 description 1
- 230000004102 tricarboxylic acid cycle Effects 0.000 description 1
- 108700026220 vif Genes Proteins 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
- C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
- C12P7/06—Ethanol, i.e. non-beverage
- C12P7/065—Ethanol, i.e. non-beverage with microorganisms other than yeasts
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0008—Oxidoreductases (1.) acting on the aldehyde or oxo group of donors (1.2)
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GBGB0605890.3A GB0605890D0 (en) | 2006-03-24 | 2006-03-24 | Enhancemeny Of Microbial Ethanol Production |
| GB0605890 | 2006-03-24 | ||
| PCT/GB2007/001060 WO2007110606A1 (en) | 2006-03-24 | 2007-03-26 | Enhancement of microbial ethanol production |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ES2396151T3 true ES2396151T3 (es) | 2013-02-19 |
Family
ID=36384080
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES07732122T Active ES2396151T3 (es) | 2006-03-24 | 2007-03-26 | Potenciamiento de la producción de etanol microbiana |
Country Status (18)
| Country | Link |
|---|---|
| US (1) | US20090226992A1 (enExample) |
| EP (1) | EP2007897B1 (enExample) |
| JP (1) | JP2009529905A (enExample) |
| CN (1) | CN101448948A (enExample) |
| AP (1) | AP2458A (enExample) |
| AU (1) | AU2007231208B2 (enExample) |
| BR (1) | BRPI0709357A2 (enExample) |
| CA (1) | CA2681380A1 (enExample) |
| CU (1) | CU23771A3 (enExample) |
| DK (1) | DK2007897T3 (enExample) |
| ES (1) | ES2396151T3 (enExample) |
| GB (1) | GB0605890D0 (enExample) |
| MX (1) | MX2008012128A (enExample) |
| NO (1) | NO20084451L (enExample) |
| NZ (1) | NZ571490A (enExample) |
| PL (1) | PL2007897T3 (enExample) |
| WO (1) | WO2007110606A1 (enExample) |
| ZA (1) | ZA200808380B (enExample) |
Families Citing this family (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB2461495A (en) * | 2008-02-13 | 2010-01-06 | Bioconversion Technologies Ltd | Ethanol production by lactate dehydrogenase-deleted thermophilic microorganisms |
| WO2009106835A2 (en) | 2008-02-28 | 2009-09-03 | Green Biologics Limited | Production process |
| US8715977B2 (en) | 2009-08-28 | 2014-05-06 | Kyoto University | Ethanol production from ocean biomass |
| JP2011167096A (ja) * | 2010-02-17 | 2011-09-01 | Kobe Univ | バイオマスからのエタノールの生産方法 |
| GB2478791A (en) * | 2010-03-19 | 2011-09-21 | Qteros Inc | Ethanol production by genetically-modified bacteria |
| CN102296079A (zh) * | 2011-01-18 | 2011-12-28 | 浙江大学 | 耐热型甲酸脱氢酶基因及其编码的多肽 |
| GB2489967A (en) * | 2011-04-13 | 2012-10-17 | Ensus Ltd | Method of producing an animal feed by hydrolysis and fermentation |
| CA2850480A1 (en) | 2011-09-30 | 2013-06-20 | Mascoma Corporation | Engineering microorganisms to increase ethanol production by metabolic redirection |
| GB201417362D0 (en) | 2014-10-01 | 2014-11-12 | Hartley Brian S | Devices and methods for selection of microbial strains |
| KR101976691B1 (ko) * | 2017-11-15 | 2019-05-09 | 울산과학기술원 | 재조합 미생물 및 이를 이용한 개미산 제조 방법 |
| CN114540404B (zh) * | 2022-02-28 | 2023-11-07 | 大连理工大学 | 一种乙醇发酵过程中二氧化碳原位固定的方法 |
| CN118685438B (zh) * | 2024-08-09 | 2025-03-04 | 微元合成生物技术(北京)有限公司 | 一种基于nad(h)辅因子循环系统合成d-木糖醇的方法及其专用工程菌 |
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|---|---|---|---|---|
| GB8516057D0 (en) * | 1985-06-25 | 1985-07-31 | Ciba Geigy Ag | Turning device |
| US5182199A (en) * | 1987-05-27 | 1993-01-26 | Hartley Brian S | Thermophilic ethanol production in a two-stage closed system |
| JP3396224B2 (ja) * | 1995-09-12 | 2003-04-14 | 麒麟麦酒株式会社 | カンジダ・ボイジニイのギ酸脱水素酵素遺伝子のプロモーター／ターミネーター |
| GB0000185D0 (en) * | 2000-01-06 | 2000-03-01 | Agrol Limited | Ethanol production |
| GB0011186D0 (en) * | 2000-05-09 | 2000-06-28 | Agrol Limited | Modification of bacteria |
| CA2424890C (en) * | 2000-10-06 | 2014-06-03 | Elsworth Biotechnology Limited | Ethanol production in gram-positive bacteria with a stabilized mutation in lactate dehydrogenase |
| CA2466133A1 (en) * | 2001-11-02 | 2003-05-15 | Rice University | Recycling system for manipulation of intracellular nadh availability |
| US7927859B2 (en) * | 2003-08-22 | 2011-04-19 | Rice University | High molar succinate yield bacteria by increasing the intracellular NADH availability |
| WO2007110592A2 (en) * | 2006-03-24 | 2007-10-04 | Elsworth Ethanol Company Limited | Fermentation process for the production of ethanol |
| CA2735883C (en) * | 2008-09-10 | 2020-05-05 | Genomatica, Inc. | Microorganisms for the production of 1,4-butanediol |
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2006
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| CU23771A3 (es) | 2012-02-15 |
| AU2007231208B2 (en) | 2012-12-13 |
| US20090226992A1 (en) | 2009-09-10 |
| DK2007897T3 (da) | 2013-01-14 |
| AP2458A (en) | 2012-09-12 |
| CN101448948A (zh) | 2009-06-03 |
| BRPI0709357A2 (pt) | 2011-07-12 |
| JP2009529905A (ja) | 2009-08-27 |
| GB0605890D0 (en) | 2006-05-03 |
| WO2007110606A1 (en) | 2007-10-04 |
| PL2007897T3 (pl) | 2013-04-30 |
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| CA2681380A1 (en) | 2007-10-04 |
| ZA200808380B (en) | 2009-11-25 |
| EP2007897A1 (en) | 2008-12-31 |
| AP2008004642A0 (en) | 2008-10-31 |
| AU2007231208A1 (en) | 2007-10-04 |
| NZ571490A (en) | 2011-09-30 |
| EP2007897B1 (en) | 2012-09-19 |
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