ES2231883T3 - Procedimiento para desarrollar mamiferos con propiedades geneticas definidas. - Google Patents
Procedimiento para desarrollar mamiferos con propiedades geneticas definidas.Info
- Publication number
- ES2231883T3 ES2231883T3 ES97936698T ES97936698T ES2231883T3 ES 2231883 T3 ES2231883 T3 ES 2231883T3 ES 97936698 T ES97936698 T ES 97936698T ES 97936698 T ES97936698 T ES 97936698T ES 2231883 T3 ES2231883 T3 ES 2231883T3
- Authority
- ES
- Spain
- Prior art keywords
- cells
- mice
- blastocysts
- embryos
- animals
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 238000000034 method Methods 0.000 title claims abstract description 59
- 230000002068 genetic effect Effects 0.000 title claims abstract description 12
- 210000004027 cell Anatomy 0.000 claims abstract description 210
- 210000002459 blastocyst Anatomy 0.000 claims abstract description 59
- 241001465754 Metazoa Species 0.000 claims abstract description 51
- 208000035199 Tetraploidy Diseases 0.000 claims abstract description 41
- 230000009261 transgenic effect Effects 0.000 claims abstract description 18
- 210000001671 embryonic stem cell Anatomy 0.000 claims abstract description 11
- 210000001161 mammalian embryo Anatomy 0.000 claims abstract description 11
- 241000699670 Mus sp. Species 0.000 claims description 61
- 210000002257 embryonic structure Anatomy 0.000 claims description 46
- 238000004519 manufacturing process Methods 0.000 claims description 24
- 238000012239 gene modification Methods 0.000 claims description 12
- 230000005017 genetic modification Effects 0.000 claims description 12
- 235000013617 genetically modified food Nutrition 0.000 claims description 12
- 239000013612 plasmid Substances 0.000 claims description 9
- 230000004927 fusion Effects 0.000 claims description 8
- 239000000969 carrier Substances 0.000 claims description 4
- 238000000520 microinjection Methods 0.000 claims description 3
- 241000894007 species Species 0.000 claims description 2
- 210000004602 germ cell Anatomy 0.000 abstract description 19
- 230000008569 process Effects 0.000 abstract description 9
- 238000000338 in vitro Methods 0.000 abstract description 7
- 241000124008 Mammalia Species 0.000 abstract description 4
- 108090000623 proteins and genes Proteins 0.000 description 33
- 210000003754 fetus Anatomy 0.000 description 29
- 238000002347 injection Methods 0.000 description 26
- 239000007924 injection Substances 0.000 description 26
- 241000699666 Mus <mouse, genus> Species 0.000 description 22
- 108010070600 Glucose-6-phosphate isomerase Proteins 0.000 description 14
- 230000014509 gene expression Effects 0.000 description 14
- 210000001519 tissue Anatomy 0.000 description 14
- 108090000123 Fos-related antigen 1 Proteins 0.000 description 13
- 238000002474 experimental method Methods 0.000 description 13
- 238000011161 development Methods 0.000 description 12
- 230000018109 developmental process Effects 0.000 description 12
- 238000004458 analytical method Methods 0.000 description 11
- 210000000472 morula Anatomy 0.000 description 11
- 239000013598 vector Substances 0.000 description 11
- 108700028369 Alleles Proteins 0.000 description 9
- 102000003817 Fos-related antigen 1 Human genes 0.000 description 8
- 108700019146 Transgenes Proteins 0.000 description 7
- 230000000694 effects Effects 0.000 description 7
- 230000015572 biosynthetic process Effects 0.000 description 6
- 101150066555 lacZ gene Proteins 0.000 description 6
- 102000007568 Proto-Oncogene Proteins c-fos Human genes 0.000 description 5
- 108010071563 Proto-Oncogene Proteins c-fos Proteins 0.000 description 5
- 102000005936 beta-Galactosidase Human genes 0.000 description 5
- 108010005774 beta-Galactosidase Proteins 0.000 description 5
- 238000010186 staining Methods 0.000 description 5
- TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 description 4
- 230000008901 benefit Effects 0.000 description 4
- 238000002744 homologous recombination Methods 0.000 description 4
- 230000006801 homologous recombination Effects 0.000 description 4
- 239000003550 marker Substances 0.000 description 4
- 102000004169 proteins and genes Human genes 0.000 description 4
- 206010068051 Chimerism Diseases 0.000 description 3
- 108010044467 Isoenzymes Proteins 0.000 description 3
- 101100351020 Mus musculus Pax5 gene Proteins 0.000 description 3
- 229910019142 PO4 Inorganic materials 0.000 description 3
- 238000002105 Southern blotting Methods 0.000 description 3
- 238000010363 gene targeting Methods 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 3
- 239000010452 phosphate Substances 0.000 description 3
- 230000029058 respiratory gaseous exchange Effects 0.000 description 3
- 238000012546 transfer Methods 0.000 description 3
- FBPFZTCFMRRESA-KVTDHHQDSA-N D-Mannitol Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-KVTDHHQDSA-N 0.000 description 2
- 108020004414 DNA Proteins 0.000 description 2
- 108091092195 Intron Proteins 0.000 description 2
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- 229930195725 Mannitol Natural products 0.000 description 2
- 229930193140 Neomycin Natural products 0.000 description 2
- 108700008625 Reporter Genes Proteins 0.000 description 2
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- 230000037396 body weight Effects 0.000 description 2
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- 230000009849 deactivation Effects 0.000 description 2
- 238000012217 deletion Methods 0.000 description 2
- 230000037430 deletion Effects 0.000 description 2
- 229940009976 deoxycholate Drugs 0.000 description 2
- KXGVEGMKQFWNSR-LLQZFEROSA-N deoxycholic acid Chemical compound C([C@H]1CC2)[C@H](O)CC[C@]1(C)[C@@H]1[C@@H]2[C@@H]2CC[C@H]([C@@H](CCC(O)=O)C)[C@@]2(C)[C@@H](O)C1 KXGVEGMKQFWNSR-LLQZFEROSA-N 0.000 description 2
- 230000004069 differentiation Effects 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 238000004520 electroporation Methods 0.000 description 2
- 230000001605 fetal effect Effects 0.000 description 2
- 230000001771 impaired effect Effects 0.000 description 2
- 238000002513 implantation Methods 0.000 description 2
- 230000010354 integration Effects 0.000 description 2
- 238000011835 investigation Methods 0.000 description 2
- 238000002955 isolation Methods 0.000 description 2
- 229910001629 magnesium chloride Inorganic materials 0.000 description 2
- 239000000594 mannitol Substances 0.000 description 2
- 235000010355 mannitol Nutrition 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000035772 mutation Effects 0.000 description 2
- 229960004927 neomycin Drugs 0.000 description 2
- 230000002018 overexpression Effects 0.000 description 2
- BASFCYQUMIYNBI-UHFFFAOYSA-N platinum Chemical compound [Pt] BASFCYQUMIYNBI-UHFFFAOYSA-N 0.000 description 2
- 230000035935 pregnancy Effects 0.000 description 2
- RXWNCPJZOCPEPQ-NVWDDTSBSA-N puromycin Chemical compound C1=CC(OC)=CC=C1C[C@H](N)C(=O)N[C@H]1[C@@H](O)[C@H](N2C3=NC=NC(=C3N=C2)N(C)C)O[C@@H]1CO RXWNCPJZOCPEPQ-NVWDDTSBSA-N 0.000 description 2
- 230000001177 retroviral effect Effects 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 238000011830 transgenic mouse model Methods 0.000 description 2
- OPIFSICVWOWJMJ-AEOCFKNESA-N 5-bromo-4-chloro-3-indolyl beta-D-galactoside Chemical compound O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@H]1OC1=CNC2=CC=C(Br)C(Cl)=C12 OPIFSICVWOWJMJ-AEOCFKNESA-N 0.000 description 1
- 102000006822 Agouti Signaling Protein Human genes 0.000 description 1
- 108010072151 Agouti Signaling Protein Proteins 0.000 description 1
- 238000011721 B6CBAF1 mouse Methods 0.000 description 1
- 108700003860 Bacterial Genes Proteins 0.000 description 1
- 240000000244 Crotalaria pallida Species 0.000 description 1
- 241000484025 Cuniculus Species 0.000 description 1
- XFXPMWWXUTWYJX-UHFFFAOYSA-N Cyanide Chemical compound N#[C-] XFXPMWWXUTWYJX-UHFFFAOYSA-N 0.000 description 1
- 208000034423 Delivery Diseases 0.000 description 1
- YQYJSBFKSSDGFO-UHFFFAOYSA-N Epihygromycin Natural products OC1C(O)C(C(=O)C)OC1OC(C(=C1)O)=CC=C1C=C(C)C(=O)NC1C(O)C(O)C2OCOC2C1O YQYJSBFKSSDGFO-UHFFFAOYSA-N 0.000 description 1
- 108700024394 Exon Proteins 0.000 description 1
- 102000005731 Glucose-6-phosphate isomerase Human genes 0.000 description 1
- SXRSQZLOMIGNAQ-UHFFFAOYSA-N Glutaraldehyde Chemical compound O=CCCCC=O SXRSQZLOMIGNAQ-UHFFFAOYSA-N 0.000 description 1
- 241000282412 Homo Species 0.000 description 1
- 101150002416 Igf2 gene Proteins 0.000 description 1
- 108090000769 Isomerases Proteins 0.000 description 1
- 102000004195 Isomerases Human genes 0.000 description 1
- 241000699660 Mus musculus Species 0.000 description 1
- 101100447536 Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) pgi-1 gene Proteins 0.000 description 1
- 108020004511 Recombinant DNA Proteins 0.000 description 1
- 101100351021 Xenopus laevis pax5 gene Proteins 0.000 description 1
- 230000006978 adaptation Effects 0.000 description 1
- 210000001691 amnion Anatomy 0.000 description 1
- 210000004102 animal cell Anatomy 0.000 description 1
- 238000010171 animal model Methods 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 230000033228 biological regulation Effects 0.000 description 1
- 229960000074 biopharmaceutical Drugs 0.000 description 1
- 210000001109 blastomere Anatomy 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000001506 calcium phosphate Substances 0.000 description 1
- 229910000389 calcium phosphate Inorganic materials 0.000 description 1
- 235000011010 calcium phosphates Nutrition 0.000 description 1
- 239000006285 cell suspension Substances 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 239000013256 coordination polymer Substances 0.000 description 1
- 230000034994 death Effects 0.000 description 1
- 230000002950 deficient Effects 0.000 description 1
- 238000006471 dimerization reaction Methods 0.000 description 1
- 210000001840 diploid cell Anatomy 0.000 description 1
- 235000013601 eggs Nutrition 0.000 description 1
- 210000001900 endoderm Anatomy 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000001973 epigenetic effect Effects 0.000 description 1
- DEFVIWRASFVYLL-UHFFFAOYSA-N ethylene glycol bis(2-aminoethyl)tetraacetic acid Chemical compound OC(=O)CN(CC(O)=O)CCOCCOCCN(CC(O)=O)CC(O)=O DEFVIWRASFVYLL-UHFFFAOYSA-N 0.000 description 1
- 230000007717 exclusion Effects 0.000 description 1
- 239000013604 expression vector Substances 0.000 description 1
- 230000035558 fertility Effects 0.000 description 1
- 230000008014 freezing Effects 0.000 description 1
- 238000007710 freezing Methods 0.000 description 1
- 230000006543 gametophyte development Effects 0.000 description 1
- 238000010353 genetic engineering Methods 0.000 description 1
- 230000012010 growth Effects 0.000 description 1
- 239000003102 growth factor Substances 0.000 description 1
- 230000002650 habitual effect Effects 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- OXNSNGFUWQVOKD-UHFFFAOYSA-N iron(2+);dicyanide Chemical compound [Fe+2].N#[C-].N#[C-] OXNSNGFUWQVOKD-UHFFFAOYSA-N 0.000 description 1
- 108700025907 jun Genes Proteins 0.000 description 1
- 238000011813 knockout mouse model Methods 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 210000004072 lung Anatomy 0.000 description 1
- 230000014759 maintenance of location Effects 0.000 description 1
- 210000004379 membrane Anatomy 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 210000001259 mesencephalon Anatomy 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 230000008520 organization Effects 0.000 description 1
- 210000003101 oviduct Anatomy 0.000 description 1
- 108010079892 phosphoglycerol kinase Proteins 0.000 description 1
- 210000002826 placenta Anatomy 0.000 description 1
- 229910052697 platinum Inorganic materials 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 238000000751 protein extraction Methods 0.000 description 1
- 229950010131 puromycin Drugs 0.000 description 1
- 239000011541 reaction mixture Substances 0.000 description 1
- 230000010837 receptor-mediated endocytosis Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 210000001082 somatic cell Anatomy 0.000 description 1
- 210000000130 stem cell Anatomy 0.000 description 1
- 208000027223 tetraploidy syndrome Diseases 0.000 description 1
- 238000010257 thawing Methods 0.000 description 1
- 230000009772 tissue formation Effects 0.000 description 1
- QORWJWZARLRLPR-UHFFFAOYSA-H tricalcium bis(phosphate) Chemical compound [Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O QORWJWZARLRLPR-UHFFFAOYSA-H 0.000 description 1
- 210000004291 uterus Anatomy 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
- 238000011816 wild-type C57Bl6 mouse Methods 0.000 description 1
- 239000002023 wood Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/027—New or modified breeds of vertebrates
- A01K67/0275—Genetically modified vertebrates, e.g. transgenic
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2217/00—Genetically modified animals
- A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Veterinary Medicine (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Engineering & Computer Science (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Saccharide Compounds (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE19632532A DE19632532A1 (de) | 1996-08-13 | 1996-08-13 | Verfahren zur Herstellung von Säugetieren mit definierten genetischen Eigenschaften |
| DE19632532 | 1996-08-13 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ES2231883T3 true ES2231883T3 (es) | 2005-05-16 |
Family
ID=7802466
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES97936698T Expired - Lifetime ES2231883T3 (es) | 1996-08-13 | 1997-08-09 | Procedimiento para desarrollar mamiferos con propiedades geneticas definidas. |
Country Status (9)
| Country | Link |
|---|---|
| US (1) | US6492575B1 (enExample) |
| EP (1) | EP0928332B1 (enExample) |
| JP (2) | JP2000516463A (enExample) |
| AT (1) | ATE281516T1 (enExample) |
| CA (1) | CA2263729C (enExample) |
| DE (2) | DE19632532A1 (enExample) |
| ES (1) | ES2231883T3 (enExample) |
| PT (1) | PT928332E (enExample) |
| WO (1) | WO1998006834A1 (enExample) |
Families Citing this family (25)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5523226A (en) * | 1993-05-14 | 1996-06-04 | Biotechnology Research And Development Corp. | Transgenic swine compositions and methods |
| CN1083687C (zh) * | 1999-02-15 | 2002-05-01 | 湖南师范大学 | 四倍体鱼的培育方法 |
| CN1419413A (zh) * | 2000-03-28 | 2003-05-21 | 路德维格癌症研究所 | 表达血小板衍生生长因子c(pdgf－c)的非人转基因动物及其应用 |
| US6784336B2 (en) * | 2000-09-20 | 2004-08-31 | Whitehead Institute For Biomedical Research | Method of producing mutant mice |
| JP4087338B2 (ja) * | 2001-11-15 | 2008-05-21 | キリンファーマ株式会社 | キメラ非ヒト動物 |
| JP4364474B2 (ja) | 2002-02-15 | 2009-11-18 | 大学共同利用機関法人情報・システム研究機構 | 哺乳動物において機能的なトランスポゾン |
| US7365182B2 (en) * | 2002-05-01 | 2008-04-29 | Michigan State University | Plant vernalization independence (VIP) genes, proteins, and methods of use |
| GB0210539D0 (en) * | 2002-05-08 | 2002-06-19 | Univ Edinburgh | Control of es cell self renewal and lineage specification, and medium therefor |
| US7723565B2 (en) * | 2004-01-07 | 2010-05-25 | Wisconsin Alumni Research Foundation | Vernalization-related molecules and methods for inducibly-conferring epigenetic changes |
| EP2064325B1 (en) | 2006-09-01 | 2011-12-07 | Therapeutic Human Polyclonals, Inc. | Enhanced expression of human or humanized immunoglobulin in non-human transgenic animals |
| JP5624891B2 (ja) * | 2007-12-31 | 2014-11-12 | バクスター・インターナショナル・インコーポレイテッドＢａｘｔｅｒ Ｉｎｔｅｒｎａｔｉｏｎａｌ Ｉｎｃｏｒｐ０Ｒａｔｅｄ | ヒト血液凝固因子を発現するトランスジェニック非ヒト動物 |
| US9587249B2 (en) | 2008-10-27 | 2017-03-07 | Baxalta GmbH | Models of thrombotic thrombocytopenic purpura and methods of use thereof |
| US20120178158A1 (en) * | 2009-06-23 | 2012-07-12 | The Scripps Research Institute | Adult animals generated from induced pluripotent cells |
| JP2013500743A (ja) | 2009-08-04 | 2013-01-10 | バクスター・インターナショナル・インコーポレイテッド | Ｆｖｉｉｉおよびｖｗｆがノックアウトされた遺伝子導入マウス−血友病ａモデル |
| US10662256B2 (en) | 2010-07-26 | 2020-05-26 | Trianni, Inc. | Transgenic mammals and methods of use thereof |
| US10793829B2 (en) | 2010-07-26 | 2020-10-06 | Trianni, Inc. | Transgenic mammals and methods of use thereof |
| AU2011286185B2 (en) | 2010-07-26 | 2014-08-14 | Trianni, Inc. | Transgenic animals and methods of use |
| WO2015013575A2 (en) * | 2013-07-25 | 2015-01-29 | Ingenious Targeting Laboratory, Inc. | Knockout mice derived from site specific recombinase |
| EP3384030A4 (en) | 2015-12-03 | 2019-07-03 | Trianni, Inc. | IMPROVED IMMUNOGLULINIVITY |
| JP6929877B2 (ja) | 2016-02-04 | 2021-09-01 | トリアニ・インコーポレイテッドＴｒｉａｎｎｉ， Ｉｎｃ． | 免疫グロブリン産生の増強 |
| MA52431A (fr) * | 2018-03-02 | 2021-01-06 | Generation Bio Co | Identification et caractérisation de zones de sécurité du génome (gsh) chez les êtres humains et les génomes murins, et compositions de vecteurs viraux et non viraux pour une intégration ciblée au niveau d'un locus gsh identifié |
| JP7578306B2 (ja) * | 2020-03-06 | 2024-11-06 | 広州明訊生物科技有限公司 | 動物の製造方法 |
| WO2022235929A1 (en) | 2021-05-05 | 2022-11-10 | Radius Pharmaceuticals, Inc. | Animal model having homologous recombination of mouse pth1 receptor |
| CN115466718B (zh) * | 2022-08-09 | 2023-07-14 | 广州明迅生物科技有限责任公司 | 雌性胚胎干细胞及其制备方法和应用 |
| CN118185862B (zh) * | 2024-05-16 | 2024-08-09 | 中国科学院昆明动物研究所 | 一种小鼠四倍体器官的制备方法 |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| NZ244306A (en) * | 1991-09-30 | 1995-07-26 | Boehringer Ingelheim Int | Composition for introducing nucleic acid complexes into eucaryotic cells, complex containing nucleic acid and endosomolytic agent, peptide with endosomolytic domain and nucleic acid binding domain and preparation |
| DE4228162C1 (de) * | 1992-08-25 | 1994-01-13 | Rajewsky Klaus Dr | Verfahren zum Ersetzen homologer Genabschnitte aus Säugern in der Keimbahn von nicht-menschlichen Säugern |
| US5523226A (en) | 1993-05-14 | 1996-06-04 | Biotechnology Research And Development Corp. | Transgenic swine compositions and methods |
| US5907080A (en) * | 1995-11-30 | 1999-05-25 | Nexia Biotechnologies, Inc. | Method for development of transgenic dwarf goats |
| US6331406B1 (en) | 1997-03-31 | 2001-12-18 | The John Hopkins University School Of Medicine | Human enbryonic germ cell and methods of use |
-
1996
- 1996-08-13 DE DE19632532A patent/DE19632532A1/de not_active Withdrawn
-
1997
- 1997-08-09 AT AT97936698T patent/ATE281516T1/de active
- 1997-08-09 JP JP10509392A patent/JP2000516463A/ja not_active Withdrawn
- 1997-08-09 WO PCT/EP1997/004352 patent/WO1998006834A1/de not_active Ceased
- 1997-08-09 DE DE59712068T patent/DE59712068D1/de not_active Expired - Lifetime
- 1997-08-09 US US09/242,406 patent/US6492575B1/en not_active Expired - Lifetime
- 1997-08-09 PT PT97936698T patent/PT928332E/pt unknown
- 1997-08-09 EP EP97936698A patent/EP0928332B1/de not_active Expired - Lifetime
- 1997-08-09 CA CA002263729A patent/CA2263729C/en not_active Expired - Lifetime
- 1997-08-09 ES ES97936698T patent/ES2231883T3/es not_active Expired - Lifetime
-
2006
- 2006-08-10 JP JP2006218386A patent/JP4129472B2/ja not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| US6492575B1 (en) | 2002-12-10 |
| ATE281516T1 (de) | 2004-11-15 |
| DE59712068D1 (de) | 2004-12-09 |
| CA2263729C (en) | 2006-06-27 |
| CA2263729A1 (en) | 1998-02-19 |
| JP4129472B2 (ja) | 2008-08-06 |
| EP0928332A1 (de) | 1999-07-14 |
| EP0928332B1 (de) | 2004-11-03 |
| PT928332E (pt) | 2005-01-31 |
| JP2000516463A (ja) | 2000-12-12 |
| WO1998006834A1 (de) | 1998-02-19 |
| US20020178457A1 (en) | 2002-11-28 |
| JP2006296440A (ja) | 2006-11-02 |
| DE19632532A1 (de) | 1998-02-19 |
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