ES2184623A1 - Piel artificial autologa secretora de leptina y metodo de obtencion. - Google Patents

Piel artificial autologa secretora de leptina y metodo de obtencion.

Info

Publication number
ES2184623A1
ES2184623A1 ES200101526A ES200101526A ES2184623A1 ES 2184623 A1 ES2184623 A1 ES 2184623A1 ES 200101526 A ES200101526 A ES 200101526A ES 200101526 A ES200101526 A ES 200101526A ES 2184623 A1 ES2184623 A1 ES 2184623A1
Authority
ES
Spain
Prior art keywords
keratinocytes
artificial
platelets
plasma
cultivating
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
ES200101526A
Other languages
English (en)
Other versions
ES2184623B1 (es
Inventor
Noval Jose Luis Jorcano
Laguzzi Fernando Larcher
Infiesta Alvaro Meana
Rio Nechaevsky Marcela Del
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
CT COMUNITARIO DE TRANSFUSUIN
Centro de Investigaciones Energeticas Medioambientales y Tecnologicas CIEMAT
Original Assignee
CT COMUNITARIO DE TRANSFUSUIN
Centro de Investigaciones Energeticas Medioambientales y Tecnologicas CIEMAT
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by CT COMUNITARIO DE TRANSFUSUIN, Centro de Investigaciones Energeticas Medioambientales y Tecnologicas CIEMAT filed Critical CT COMUNITARIO DE TRANSFUSUIN
Priority to ES200101526A priority Critical patent/ES2184623B1/es
Priority to PCT/ES2002/000320 priority patent/WO2003002154A1/es
Publication of ES2184623A1 publication Critical patent/ES2184623A1/es
Application granted granted Critical
Publication of ES2184623B1 publication Critical patent/ES2184623B1/es
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0625—Epidermal cells, skin cells; Cells of the oral mucosa
    • C12N5/0629—Keratinocytes; Whole skin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P5/00—Drugs for disorders of the endocrine system
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0697—Artificial constructs associating cells of different lineages, e.g. tissue equivalents
    • C12N5/0698—Skin equivalents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61F—FILTERS IMPLANTABLE INTO BLOOD VESSELS; PROSTHESES; DEVICES PROVIDING PATENCY TO, OR PREVENTING COLLAPSING OF, TUBULAR STRUCTURES OF THE BODY, e.g. STENTS; ORTHOPAEDIC, NURSING OR CONTRACEPTIVE DEVICES; FOMENTATION; TREATMENT OR PROTECTION OF EYES OR EARS; BANDAGES, DRESSINGS OR ABSORBENT PADS; FIRST-AID KITS
    • A61F2/00—Filters implantable into blood vessels; Prostheses, i.e. artificial substitutes or replacements for parts of the body; Appliances for connecting them with the body; Devices providing patency to, or preventing collapsing of, tubular structures of the body, e.g. stents
    • A61F2/02—Prostheses implantable into the body
    • A61F2/10—Hair or skin implants
    • A61F2/105—Skin implants, e.g. artificial skin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/09—Coculture with; Conditioned medium produced by epidermal cells, skin cells, oral mucosa cells
    • C12N2502/094—Coculture with; Conditioned medium produced by epidermal cells, skin cells, oral mucosa cells keratinocytes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/13—Coculture with; Conditioned medium produced by connective tissue cells; generic mesenchyme cells, e.g. so-called "embryonic fibroblasts"
    • C12N2502/1323—Adult fibroblasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Animal Behavior & Ethology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Diabetes (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Endocrinology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Dermatology (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Materials For Medical Uses (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Piel artificial autóloga secretora de leptina y método de obtención. Incorpora queratinocitos modificados genéticamente mediante su infección con un vector retroviral portador del gen codificante de la leptina humana sobre una dermis artificial autóloga obtenida, básicamente, por la gelificación con Ca{sup,++} de plasma con plaquetas. El método de obtención comprende las etapas de: - Obtención del plasma con plaquetas mediante centrifugación ligera. - Cultivo de células dérmicas a partir de una biopsia. - Gelificación del plasma con plaquetas mediante sales de calcio, sembrando en el interior de la matriz de fibrina las células dérmicas cultivadas. - Cultivo de queratinocitos a partir de una biopsia. - Modificación genética de los queratinocitos mediante infección con un vector retroviral portador del gen codificante de la leptina humana. - Cultivo de los queratinocitos modificados genéticamente hasta confluencia. - Selección de los queratinocitos modificados genéticamente mediante un gen marcador. - Sembrado de los queratinocitos seleccionados sobre la dermis artificial obtenida tras las tres primeras etapas.
ES200101526A 2001-06-29 2001-06-29 Piel artificial autologa secretora de leptina y metodo de obtencion. Expired - Fee Related ES2184623B1 (es)

Priority Applications (2)

Application Number Priority Date Filing Date Title
ES200101526A ES2184623B1 (es) 2001-06-29 2001-06-29 Piel artificial autologa secretora de leptina y metodo de obtencion.
PCT/ES2002/000320 WO2003002154A1 (es) 2001-06-29 2002-06-28 Piel artificial autologa secretora de leptina y metodo de obtencion

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ES200101526A ES2184623B1 (es) 2001-06-29 2001-06-29 Piel artificial autologa secretora de leptina y metodo de obtencion.

Publications (2)

Publication Number Publication Date
ES2184623A1 true ES2184623A1 (es) 2003-04-01
ES2184623B1 ES2184623B1 (es) 2004-09-16

Family

ID=8498249

Family Applications (1)

Application Number Title Priority Date Filing Date
ES200101526A Expired - Fee Related ES2184623B1 (es) 2001-06-29 2001-06-29 Piel artificial autologa secretora de leptina y metodo de obtencion.

Country Status (2)

Country Link
ES (1) ES2184623B1 (es)
WO (1) WO2003002154A1 (es)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2551143A1 (es) * 2014-05-13 2015-11-16 Fundación Tekniker Dermis artificial, piel artificial, métodos para su preparación y sus usos

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2173812B1 (es) * 2001-03-01 2003-12-16 Ct Investig Energeticas Ciemat Dermis artificial y metodo de obtencion.
US7468242B2 (en) 2001-11-05 2008-12-23 Medgenics, Inc. Dermal micro organs, methods and apparatuses for producing and using the same
US8088568B2 (en) 2001-11-05 2012-01-03 Medgentics, Inc. Dermal micro-organs, methods and apparatuses for producing and using the same
US8501396B2 (en) 2001-11-05 2013-08-06 Medgenics Medical Israel Ltd. Dermal micro-organs, methods and apparatuses for producing and using the same
WO2004099363A2 (en) 2003-05-01 2004-11-18 Medgenics Inc. Dermal micro organs, methods and apparatuses for producing and using the same
ATE529125T1 (de) 2003-08-07 2011-11-15 Healor Ltd Pharmazeutische zusammensetzungen und verfahren zur beschleunigung der wundheilung
DE10341533A1 (de) 2003-09-09 2005-03-31 Bayer Chemicals Ag Verfahren zur Herstellung von halogenhaltigen 4-Aminophenolen
ES2263382B1 (es) 2005-05-16 2007-11-16 Fundacion Para La Investigacion Biomedica Del Hospital Gregorio Marañon Matriz artificial de gel de fibrina endotelizada superproductora de factores proangiogenicos.
CA2625674C (en) 2005-08-29 2015-04-14 Healor Ltd. Methods and compositions for prevention and treatment of diabetic and aged skin
KR101449587B1 (ko) 2006-09-14 2014-10-10 메드제닉스 메디칼 이스라엘 리미티드 장기 지속형 약물 제형
US8454948B2 (en) 2006-09-14 2013-06-04 Medgenics Medical Israel Ltd. Long lasting drug formulations
CA2789972A1 (en) 2010-01-11 2011-07-14 Healor Ltd. Method for treatment of inflammatory disease and disorder
CN103260649A (zh) 2010-06-15 2013-08-21 迈德詹尼克斯医疗以色列有限公司 长效药物制剂

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1987000201A1 (en) * 1985-07-05 1987-01-15 Whitehead Institute For Biomedical Research Epithelial cells expressing foreign genetic material
ES2132027A1 (es) * 1997-07-04 1999-08-01 Comunitario De Transfusion Del Desarrollo de una piel artificial mediante cultivo de queratinocitos sobre una base de fibrina y fibroblastos humanos y metodo de preparacion de esta piel para trasplante.
US6001816A (en) * 1996-06-20 1999-12-14 Merck & Co., Inc. Gene therapy for leptin deficiency

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1987000201A1 (en) * 1985-07-05 1987-01-15 Whitehead Institute For Biomedical Research Epithelial cells expressing foreign genetic material
US6001816A (en) * 1996-06-20 1999-12-14 Merck & Co., Inc. Gene therapy for leptin deficiency
ES2132027A1 (es) * 1997-07-04 1999-08-01 Comunitario De Transfusion Del Desarrollo de una piel artificial mediante cultivo de queratinocitos sobre una base de fibrina y fibroblastos humanos y metodo de preparacion de esta piel para trasplante.

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
LARCHER, F. et al.: "A cutaneous gene therapy approach to human leptin deficiencies: correction of the murine ob/ob phenotype using leptin targeted keratinocyte grafts", julio 2001, FASEB Journal, Vol. 15 (9), pßginas 1529-1538, ISSN 0892-6638, todo el documento. *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2551143A1 (es) * 2014-05-13 2015-11-16 Fundación Tekniker Dermis artificial, piel artificial, métodos para su preparación y sus usos

Also Published As

Publication number Publication date
ES2184623B1 (es) 2004-09-16
WO2003002154A1 (es) 2003-01-09

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