NZ506510A - A permanent skin replacement comprising allogenic epithelial cells cultured in vitro and autologous epithelial cells - Google Patents
A permanent skin replacement comprising allogenic epithelial cells cultured in vitro and autologous epithelial cellsInfo
- Publication number
- NZ506510A NZ506510A NZ506510A NZ50651099A NZ506510A NZ 506510 A NZ506510 A NZ 506510A NZ 506510 A NZ506510 A NZ 506510A NZ 50651099 A NZ50651099 A NZ 50651099A NZ 506510 A NZ506510 A NZ 506510A
- Authority
- NZ
- New Zealand
- Prior art keywords
- epithelial cells
- vitro
- dermal
- replacement
- skin
- Prior art date
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0697—Artificial constructs associating cells of different lineages, e.g. tissue equivalents
- C12N5/0698—Skin equivalents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
- A61L27/60—Materials for use in artificial skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P17/00—Drugs for dermatological disorders
- A61P17/02—Drugs for dermatological disorders for treating wounds, ulcers, burns, scars, keloids, or the like
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2502/00—Coculture with; Conditioned medium produced by
- C12N2502/09—Coculture with; Conditioned medium produced by epidermal cells, skin cells, oral mucosa cells
- C12N2502/094—Coculture with; Conditioned medium produced by epidermal cells, skin cells, oral mucosa cells keratinocytes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2502/00—Coculture with; Conditioned medium produced by
- C12N2502/13—Coculture with; Conditioned medium produced by connective tissue cells; generic mesenchyme cells, e.g. so-called "embryonic fibroblasts"
- C12N2502/1323—Adult fibroblasts
Landscapes
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Biomedical Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Veterinary Medicine (AREA)
- Medicinal Chemistry (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- General Chemical & Material Sciences (AREA)
- Biochemistry (AREA)
- Cell Biology (AREA)
- Pharmacology & Pharmacy (AREA)
- Microbiology (AREA)
- Dermatology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- General Engineering & Computer Science (AREA)
- Oral & Maxillofacial Surgery (AREA)
- Transplantation (AREA)
- Epidemiology (AREA)
- Materials For Medical Uses (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Skin replacements to aid wound healing are described. In one embodiment, the living skin replacement is chimeric and is produced by seeding autologous epithelial cells onto a biocompatible substrate containing allogeneic epithelial cells cultured in vitro. The living chimeric skin replacement may be implanted over a permanent dermal replacement previously implanted at the wound site. In another embodiment the skin replacement is a composite comprising an inner, middle and outer component, comprising (a) an inner component comprising a biocompatible dermal construct having a biodegradable or removable scaffold as a base; (b) a middle component comprising epithelial cells; and (c) an outer component comprising epithelial cells cultured in vitro on a dermal construct comprising a dermal portion having a biodegradable or removable scaffold as a base, the dermal portion being combined with a transitional covering and facing inward toward the middle component of epithelial cells. Further described are methods of making the various skin replacements.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US7570498P | 1998-02-24 | 1998-02-24 | |
PCT/US1999/003859 WO1999043787A2 (en) | 1998-02-24 | 1999-02-23 | A living chimeric skin replacement |
Publications (1)
Publication Number | Publication Date |
---|---|
NZ506510A true NZ506510A (en) | 2003-03-28 |
Family
ID=22127475
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
NZ506510A NZ506510A (en) | 1998-02-24 | 1999-02-23 | A permanent skin replacement comprising allogenic epithelial cells cultured in vitro and autologous epithelial cells |
Country Status (7)
Country | Link |
---|---|
EP (1) | EP1062322A2 (en) |
JP (1) | JP2002504412A (en) |
KR (1) | KR20010072553A (en) |
AU (1) | AU760470B2 (en) |
CA (1) | CA2324452A1 (en) |
NZ (1) | NZ506510A (en) |
WO (1) | WO1999043787A2 (en) |
Families Citing this family (15)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6964869B2 (en) | 1998-07-13 | 2005-11-15 | Wisconsin Alumni Research Foundation | Method and composition for skin grafts |
DE19949290A1 (en) * | 1999-10-12 | 2001-04-26 | Albrecht Bettermann | Particulate construct for use in transplantation medicine |
DE10109136A1 (en) * | 2001-02-26 | 2002-09-12 | Cytotools Gmbh | Using inhibitor of thrombospondin-1 interaction for promoting wound healing, also for treating living skin replacements, inhibits apoptosis of wound-repair cells |
EP1390754B1 (en) * | 2001-04-24 | 2007-08-01 | Wisconsin Alumni Research Foundation | Method and composition for skin grafts |
US9597395B2 (en) | 2001-12-07 | 2017-03-21 | Cytori Therapeutics, Inc. | Methods of using adipose tissue-derived cells in the treatment of cardiovascular conditions |
US20050048035A1 (en) | 2001-12-07 | 2005-03-03 | Fraser John K. | Methods of using regenerative cells in the treatment of stroke and related diseases and disorders |
US20050095228A1 (en) | 2001-12-07 | 2005-05-05 | Fraser John K. | Methods of using regenerative cells in the treatment of peripheral vascular disease and related disorders |
US7771716B2 (en) | 2001-12-07 | 2010-08-10 | Cytori Therapeutics, Inc. | Methods of using regenerative cells in the treatment of musculoskeletal disorders |
US7741116B2 (en) * | 2002-03-06 | 2010-06-22 | University Of Cincinnati | Surgical device for skin therapy or testing |
GB0305976D0 (en) * | 2003-03-14 | 2003-04-23 | Wild Andrew M | Surgical clip |
WO2007061058A1 (en) * | 2005-11-24 | 2007-05-31 | Osaka University | Stimuli-responsive degradable gel |
EP2222159B1 (en) * | 2007-11-20 | 2018-02-21 | Pioneer Surgical Orthobiologics, Inc. | Cryopreservation of cells using cross-linked bioactive hydrogel matrix particles |
WO2010021993A1 (en) | 2008-08-19 | 2010-02-25 | Cytori Therapeutics, Inc. | Methods of using adipose tissue-derived cells in the treatment of the lymphatic system and malignant disease |
WO2016054592A1 (en) * | 2014-10-03 | 2016-04-07 | Cytori Therapeutics, Inc. | Use of regenerative cells in mitigating burn progression and improving skin graft incorporation and healing |
BR112018008361A2 (en) * | 2015-10-29 | 2018-10-23 | Glaxosmithkline Consumer Healthcare Holdings | composition, and method for moistening, and protecting, repairing or restoring the skin's lipid barrier. |
Family Cites Families (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5460939A (en) * | 1986-04-18 | 1995-10-24 | Advanced Tissue Sciences, Inc. | Temporary living skin replacement |
US5610007A (en) * | 1993-01-21 | 1997-03-11 | Universite Laval | Chimeric sheets of epithelial cells |
US5693332C1 (en) * | 1995-08-11 | 2001-01-09 | Univ California | Human keratinocytes supported on a hydrophilic membrane and methods of using same to effect wound closure |
WO1997008295A1 (en) * | 1995-08-25 | 1997-03-06 | Lifecell Corporation | Reconstituted skin |
-
1999
- 1999-02-23 JP JP2000533527A patent/JP2002504412A/en active Pending
- 1999-02-23 AU AU33077/99A patent/AU760470B2/en not_active Ceased
- 1999-02-23 EP EP99936092A patent/EP1062322A2/en not_active Withdrawn
- 1999-02-23 CA CA002324452A patent/CA2324452A1/en not_active Abandoned
- 1999-02-23 NZ NZ506510A patent/NZ506510A/en unknown
- 1999-02-23 KR KR1020007009299A patent/KR20010072553A/en not_active Application Discontinuation
- 1999-02-23 WO PCT/US1999/003859 patent/WO1999043787A2/en not_active Application Discontinuation
Also Published As
Publication number | Publication date |
---|---|
CA2324452A1 (en) | 1999-09-02 |
JP2002504412A (en) | 2002-02-12 |
AU3307799A (en) | 1999-09-15 |
WO1999043787A3 (en) | 1999-11-25 |
EP1062322A2 (en) | 2000-12-27 |
KR20010072553A (en) | 2001-07-31 |
AU760470B2 (en) | 2003-05-15 |
WO1999043787A2 (en) | 1999-09-02 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
RENW | Renewal (renewal fees accepted) | ||
PSEA | Patent sealed | ||
ASS | Change of ownership |
Owner name: T. J. SMITH AND NEPHEW LIMITED, GB Free format text: OLD OWNER(S): ADVANCED TISSUE SCIENCES, INC. |