CY1111727T1 - Βλαστοκυτταρα, τα οποια λαμβανονται απο πολφο νεογιλων ή μονιμων οδοντων και οδοντικους βλαστους, ικανα να παραγουν ανθρωπινο οστικο ιστο - Google Patents

Βλαστοκυτταρα, τα οποια λαμβανονται απο πολφο νεογιλων ή μονιμων οδοντων και οδοντικους βλαστους, ικανα να παραγουν ανθρωπινο οστικο ιστο

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Publication number
CY1111727T1
CY1111727T1 CY20111100492T CY111100492T CY1111727T1 CY 1111727 T1 CY1111727 T1 CY 1111727T1 CY 20111100492 T CY20111100492 T CY 20111100492T CY 111100492 T CY111100492 T CY 111100492T CY 1111727 T1 CY1111727 T1 CY 1111727T1
Authority
CY
Cyprus
Prior art keywords
dental
bone tissue
osteoblasts
vitro
isolation
Prior art date
Application number
CY20111100492T
Other languages
English (en)
Original Assignee
Teslab S.R.L.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Teslab S.R.L. filed Critical Teslab S.R.L.
Publication of CY1111727T1 publication Critical patent/CY1111727T1/el

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Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
    • A61L27/3804—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells characterised by specific cells or progenitors thereof, e.g. fibroblasts, connective tissue cells, kidney cells
    • A61L27/3821—Bone-forming cells, e.g. osteoblasts, osteocytes, osteoprogenitor cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P19/00—Drugs for skeletal disorders
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
    • C12N5/0664—Dental pulp stem cells, Dental follicle stem cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
    • A61L27/3839—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells characterised by the site of application in the body
    • A61L27/3843—Connective tissue
    • A61L27/3847—Bones
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2509/00—Methods for the dissociation of cells, e.g. specific use of enzymes

Abstract

Εις την εφεύρεση αυτή περιγράφεται μια μέθοδος, η οποία προβλέπει την απομόνωση ενός νέου υποπληθυσμού βλαστοκυττάρων που παράγονται από οδοντικό πολφό, η διαφοροποίηση των οποίων προς οστεοβλάστες οδηγεί σε μια επακόλουθη παραγωγή και εφαρμογή οστικού ιστού, που καλείται LAB (ζωντανό αυτόλογο οστό). Ειδικά η εφεύρεση περιγράφει: 1. Την απομόνωση βλαστοκυττάρων από τον πολφό νεογιλών και μονίμων οδόντων και οδοντικών βλαστών που λαμβάνονται από ανθρώπινα υποκείμενα. 2. Την ανάπτυξη των κυττάρων αυτών in vitro υπό ειδικές συνθήκες που επιτρέπουν την απομόνωση ενός κυτταρικού υποπληθυσμού, ο οποίος μετά διαφοροποίηση προς οστεοβλάστες είναι ικανός να παράγει in vitro μια εξωκυτταρική θεμέλια ουσία, ταυτόσημη προς αυτήν που ανιχνεύεται στον οστικό ιστό. 3. Την χρήση αυτού του επιλεγμένου και διαφοροποιημένου κυτταρικού πληθυσμού προκειμένου να παράγει αυτόλογο οστικό ιστό in vitro, ο οποίος περιέχει ζωτικούς οστεοβλάστες. 4. Την προφύλαξη των LAB υπό συνθήκες, οι οποίες εξασφαλίζουν κυτταρική ζωτικότητα: 5. Χρήση των LAB σε ασθενείς δότες ώστε να επανακατασκευασθεί οστικός ιστός, όπως απαιτείται εις την καθημερινή πρακτική εις την οδοντιατρική γναθοπροσωπική χειρουργική και ορθοπεδική.
CY20111100492T 2004-07-28 2011-05-20 Βλαστοκυτταρα, τα οποια λαμβανονται απο πολφο νεογιλων ή μονιμων οδοντων και οδοντικους βλαστους, ικανα να παραγουν ανθρωπινο οστικο ιστο CY1111727T1 (el)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
IT000043A ITNA20040043A1 (it) 2004-07-28 2004-07-28 Tecnica di ingegneria tissutale ottenibile mediante isolamento di una nuova sottopopolazione di cellule staminali mbp-shed ed mbp-dpsc, isolate da polpa di denti decidui e permanenti in grado di produrre in vitro tessuto osseo umano.
EP05764069A EP1773987B1 (en) 2004-07-28 2005-07-27 Stem cells obtained from pulp of deciduous or permanent teeth and of dental germ, able to produce human bone tissue

Publications (1)

Publication Number Publication Date
CY1111727T1 true CY1111727T1 (el) 2015-10-07

Family

ID=35786558

Family Applications (1)

Application Number Title Priority Date Filing Date
CY20111100492T CY1111727T1 (el) 2004-07-28 2011-05-20 Βλαστοκυτταρα, τα οποια λαμβανονται απο πολφο νεογιλων ή μονιμων οδοντων και οδοντικους βλαστους, ικανα να παραγουν ανθρωπινο οστικο ιστο

Country Status (14)

Country Link
US (1) US7955850B2 (el)
EP (1) EP1773987B1 (el)
JP (1) JP5179178B2 (el)
AT (1) ATE503006T1 (el)
BR (1) BRPI0513773A8 (el)
CY (1) CY1111727T1 (el)
DE (1) DE602005027076D1 (el)
DK (1) DK1773987T3 (el)
ES (1) ES2361527T3 (el)
IT (1) ITNA20040043A1 (el)
PL (1) PL1773987T3 (el)
PT (1) PT1773987E (el)
SI (1) SI1773987T1 (el)
WO (1) WO2006010600A2 (el)

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DE602005012081D1 (de) * 2005-07-29 2009-02-12 Univ Bonn Verfahren zur Isolierung von Stammzellen aus ein kissenformiges Gewebe der Zähne
DE102007005946A1 (de) * 2007-02-01 2008-08-14 Stiftung Caesar Therapeutische Zusammensetzung und Verwendung einer zellfreien Substanz
EP2155860B1 (en) 2007-05-03 2014-08-27 The Brigham and Women's Hospital, Inc. Multipotent stem cells and uses thereof
US8574567B2 (en) 2007-05-03 2013-11-05 The Brigham And Women's Hospital, Inc. Multipotent stem cells and uses thereof
US20110236977A1 (en) * 2008-04-02 2011-09-29 The Trustees Of Columbia University In The City Of New York Dental stem cell differentiation
ITRM20080342A1 (it) 2008-06-26 2009-12-27 Univ Degli Studi Udine Cellule di polpa dentale midollo-simili, metodi per isolamento ed uso.
WO2010026656A1 (ja) * 2008-09-05 2010-03-11 株式会社乳歯幹細胞バンク 再生医療に活用する良好な乳歯幹細胞を採取し長期間安定して冷凍保存するための総合的管理システム
US10328103B2 (en) 2009-01-03 2019-06-25 Ray C. Wasielewski Medical treatment composition comprising mammalian dental pulp stem cells
US20100172951A1 (en) * 2009-01-03 2010-07-08 Ray Wasielewski Enhanced Medical Implant
US8470308B2 (en) * 2009-01-03 2013-06-25 Ray C. Wasielewski Enhanced medical implant comprising disrupted tooth pulp and tooth particles
US11078248B2 (en) * 2010-03-19 2021-08-03 Lifenet Health BMP peptides and methods of use
JPWO2011118795A1 (ja) * 2010-03-26 2013-07-04 国立大学法人名古屋大学 損傷部治療用組成物
AU2013216173B2 (en) * 2012-02-01 2017-02-16 Organ Technologies, Inc. Dental implant and method for producing same
WO2013146992A1 (ja) 2012-03-29 2013-10-03 日本ケミカルリサーチ株式会社 歯髄由来の多能性幹細胞の製造方法
BR102013000871A2 (pt) * 2013-01-14 2014-08-19 Jose Ricardo Muniz Ferreira Processo de coleta para congelamento e armazenamento de células tronco a partir da polpa de dentes decíduos
WO2017079813A1 (pt) * 2015-11-10 2017-05-18 Muniz Ferreira José Ricardo Processo de tratamento de células tronco provenientes da polpa de dentes decíduos com posterior criopreservação e descongelamento
WO2018038180A1 (ja) 2016-08-24 2018-03-01 国立大学法人名古屋大学 精子活性化剤及びその用途
BR102016023038A2 (pt) * 2016-10-03 2018-05-02 Ricardo Muniz Ferreira José Processo de criopreservação de células-tronco obtidas a partir da polpa de dentes decíduos
IT201700052399A1 (it) * 2017-06-06 2018-12-06 Costantino Santacroce Nuova metodica di separazione e crioconservazione della polpa dentaria per ottenere cellule staminali mesenchimali multipotenti
CN107475189B (zh) * 2017-10-12 2020-12-22 北京唐颐惠康生物医学技术有限公司 一种牙髓干细胞采集套装
CA3103769A1 (en) 2018-07-31 2020-02-06 Jcr Pharmaceuticals Co., Ltd. Method for producing dental pulp-derived cells
US20230086254A1 (en) 2020-01-30 2023-03-23 Jcr Pharmaceuticals Co., Ltd. Medicinal composition comprising dental pulp-derived cells
CN112239744B (zh) * 2020-10-20 2021-08-03 青岛市妇女儿童医院（青岛市妇幼保健院、青岛市残疾儿童医疗康复中心、青岛市新生儿疾病筛查中心） 一种能够促进牙髓干细胞成骨转化的干扰rna及其应用

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US5306305A (en) * 1992-01-31 1994-04-26 Etex Corporation Methods of coating implants with bony structure
AU774581B2 (en) * 1998-08-31 2004-07-01 New York University Stem cells bearing an FGF receptor on the cell surface
CA2416801C (en) * 2000-07-21 2012-01-03 The Government Of The United States Of America, Represented By The Secretary, Department Of Health And Human Services Adult human dental pulp stem cells in vitro and in vivo
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Also Published As

Publication number Publication date
ITNA20040043A1 (it) 2004-10-28
ATE503006T1 (de) 2011-04-15
DE602005027076D1 (de) 2011-05-05
PT1773987E (pt) 2011-05-25
WO2006010600A3 (en) 2006-06-22
PL1773987T3 (pl) 2011-08-31
EP1773987B1 (en) 2011-03-23
US7955850B2 (en) 2011-06-07
EP1773987A2 (en) 2007-04-18
BRPI0513773A8 (pt) 2018-05-08
ES2361527T3 (es) 2011-06-17
JP2008507962A (ja) 2008-03-21
DK1773987T3 (da) 2011-06-06
SI1773987T1 (sl) 2011-07-29
US20090035376A1 (en) 2009-02-05
JP5179178B2 (ja) 2013-04-10
WO2006010600A2 (en) 2006-02-02
BRPI0513773A (pt) 2008-05-13

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