IT201700052399A1 - Nuova metodica di separazione e crioconservazione della polpa dentaria per ottenere cellule staminali mesenchimali multipotenti - Google Patents

Nuova metodica di separazione e crioconservazione della polpa dentaria per ottenere cellule staminali mesenchimali multipotenti

Info

Publication number
IT201700052399A1
IT201700052399A1 IT102017000052399A IT201700052399A IT201700052399A1 IT 201700052399 A1 IT201700052399 A1 IT 201700052399A1 IT 102017000052399 A IT102017000052399 A IT 102017000052399A IT 201700052399 A IT201700052399 A IT 201700052399A IT 201700052399 A1 IT201700052399 A1 IT 201700052399A1
Authority
IT
Italy
Prior art keywords
cryopreservation
separation
stem cells
mesenchymal stem
new method
Prior art date
Application number
IT102017000052399A
Other languages
English (en)
Inventor
Costantino Santacroce
Vincenzo Mattei
Stefano Martellucci
Original Assignee
Costantino Santacroce
Vincenzo Mattei
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Costantino Santacroce, Vincenzo Mattei filed Critical Costantino Santacroce
Priority to IT102017000052399A priority Critical patent/IT201700052399A1/it
Publication of IT201700052399A1 publication Critical patent/IT201700052399A1/it

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
    • C12N5/0664—Dental pulp stem cells, Dental follicle stem cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/60—Buffer, e.g. pH regulation, osmotic pressure
    • C12N2500/62—DMSO
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2509/00—Methods for the dissociation of cells, e.g. specific use of enzymes
    • C12N2509/10—Mechanical dissociation

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Developmental Biology & Embryology (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Rheumatology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
IT102017000052399A 2017-06-06 2017-06-06 Nuova metodica di separazione e crioconservazione della polpa dentaria per ottenere cellule staminali mesenchimali multipotenti IT201700052399A1 (it)

Priority Applications (1)

Application Number Priority Date Filing Date Title
IT102017000052399A IT201700052399A1 (it) 2017-06-06 2017-06-06 Nuova metodica di separazione e crioconservazione della polpa dentaria per ottenere cellule staminali mesenchimali multipotenti

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
IT102017000052399A IT201700052399A1 (it) 2017-06-06 2017-06-06 Nuova metodica di separazione e crioconservazione della polpa dentaria per ottenere cellule staminali mesenchimali multipotenti

Publications (1)

Publication Number Publication Date
IT201700052399A1 true IT201700052399A1 (it) 2018-12-06

Family

ID=59974762

Family Applications (1)

Application Number Title Priority Date Filing Date
IT102017000052399A IT201700052399A1 (it) 2017-06-06 2017-06-06 Nuova metodica di separazione e crioconservazione della polpa dentaria per ottenere cellule staminali mesenchimali multipotenti

Country Status (1)

Country Link
IT (1) IT201700052399A1 (it)

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2006010600A2 (en) * 2004-07-28 2006-02-02 Francesco Carinci Stem cells obtained from pulp of deciduous or permanent teeth and of dental germ, able to produce human bone tissue
WO2014107784A1 (pt) * 2013-01-14 2014-07-17 Muniz Ferreira Jose Ricardo Processo de coleta para congelamento e armazenamento de células tronco a partir da polpa de dentes decíduos

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2006010600A2 (en) * 2004-07-28 2006-02-02 Francesco Carinci Stem cells obtained from pulp of deciduous or permanent teeth and of dental germ, able to produce human bone tissue
WO2014107784A1 (pt) * 2013-01-14 2014-07-17 Muniz Ferreira Jose Ricardo Processo de coleta para congelamento e armazenamento de células tronco a partir da polpa de dentes decíduos

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
EDGAR LEDESMA-MARTÍNEZ ET AL: "Mesenchymal Stem Cells Derived from Dental Pulp: A Review", STEM CELLS INTERNATIONAL, vol. 2016, 1 January 2016 (2016-01-01), US, pages 1 - 12, XP055427990, ISSN: 1687-966X, DOI: 10.1155/2016/4709572 *
JEON MIJEONG ET AL: "In vitro and in vivo characteristics of stem cells from human exfoliated deciduous teeth obtained by enzymatic disaggregation and outgrowth", ARCHIVES OF ORAL BIOLOGY, PERGAMON PRESS, OXFORD, GB, vol. 59, no. 10, 11 June 2014 (2014-06-11), pages 1013 - 1023, XP029007451, ISSN: 0003-9969, DOI: 10.1016/J.ARCHORALBIO.2014.06.002 *
LINDEMANN DANIELE ET AL: "Effects of cryopreservation on the characteristics of dental pulp stem cells of intact deciduous teeth", ARCHIVES OF ORAL BIOLOGY, vol. 59, no. 9, 1 April 2014 (2014-04-01), pages 970 - 976, XP028861576, ISSN: 0003-9969, DOI: 10.1016/J.ARCHORALBIO.2014.04.008 *
NAN ZHANG ET AL: "Isolation, characterization and multi-lineage differentiation of stem cells from human exfoliated deciduous teeth", MOLECULAR MEDICINE REPORTS, vol. 14, no. 1, 5 May 2016 (2016-05-05), GR, pages 95 - 102, XP055427989, ISSN: 1791-2997, DOI: 10.3892/mmr.2016.5214 *
WEIBO ZHANG ET AL: "Multilineage Differentiation Potential of Stem Cells Derived from Human Dental Pulp after Cryopreservation", TISSUE ENGINEERING, vol. 12, no. 10, 1 October 2006 (2006-10-01), US, pages 2813 - 2823, XP055428047, ISSN: 1076-3279, DOI: 10.1089/ten.2006.12.2813 *

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