ES2132027A1 - Desarrollo de una piel artificial mediante cultivo de queratinocitos sobre una base de fibrina y fibroblastos humanos y metodo de preparacion de esta piel para trasplante. - Google Patents
Desarrollo de una piel artificial mediante cultivo de queratinocitos sobre una base de fibrina y fibroblastos humanos y metodo de preparacion de esta piel para trasplante.Info
- Publication number
- ES2132027A1 ES2132027A1 ES09701533A ES9701533A ES2132027A1 ES 2132027 A1 ES2132027 A1 ES 2132027A1 ES 09701533 A ES09701533 A ES 09701533A ES 9701533 A ES9701533 A ES 9701533A ES 2132027 A1 ES2132027 A1 ES 2132027A1
- Authority
- ES
- Spain
- Prior art keywords
- skin
- artificial skin
- transplantation
- base
- fibrin
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
- A61L27/60—Materials for use in artificial skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/14—Macromolecular materials
- A61L27/22—Polypeptides or derivatives thereof, e.g. degradation products
- A61L27/225—Fibrin; Fibrinogen
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Dermatology (AREA)
- Medicinal Chemistry (AREA)
- Oral & Maxillofacial Surgery (AREA)
- Transplantation (AREA)
- Epidemiology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Materials For Medical Uses (AREA)
Abstract
Desarrollo de una piel artificial mediante cultivo de queratinocitos sobre una base de fibrina y fibroblastos humanos y método de preparación de esta piel para trasplante. Piel artificial obtenida mediante cultivo celular de queratinocitos sobre una base constituida por un gel de fibrina humana poblado de fibroblastos humanos. Esta base permite un desarrollo muy rápido de los queratinocitos, detectándose en esta piel artificial proteínas de la unión dermoepidérmica que facilitan el posterior éxito de trasplante. Esta piel artificial precisa, para ser utilizada en la clínica, una preparación previa que consiste en la fijación a un soporte sólido y un sistema que posibilite el transporte hasta el lugar de trasplante sin rotura de la misma. La piel artificial aquí descrita puede servir para el tratamiento de grandes quemados, úlceras cutáneas crónicas o utilizarse, mediante el empleo de células genéticamente modificadas, como base para terapia génica.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
ES9701533A ES2132027B1 (es) | 1997-07-04 | 1997-07-04 | Desarrollo de una piel artificial mediante cultivo de queratinocitos sobre una base de fibrina y fibroblastos humanos y metodo de preparacion de esta piel para trasplante. |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
ES9701533A ES2132027B1 (es) | 1997-07-04 | 1997-07-04 | Desarrollo de una piel artificial mediante cultivo de queratinocitos sobre una base de fibrina y fibroblastos humanos y metodo de preparacion de esta piel para trasplante. |
Publications (2)
Publication Number | Publication Date |
---|---|
ES2132027A1 true ES2132027A1 (es) | 1999-08-01 |
ES2132027B1 ES2132027B1 (es) | 2000-04-01 |
Family
ID=8300013
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES9701533A Expired - Fee Related ES2132027B1 (es) | 1997-07-04 | 1997-07-04 | Desarrollo de una piel artificial mediante cultivo de queratinocitos sobre una base de fibrina y fibroblastos humanos y metodo de preparacion de esta piel para trasplante. |
Country Status (1)
Country | Link |
---|---|
ES (1) | ES2132027B1 (es) |
Cited By (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2002072800A1 (es) * | 2001-03-01 | 2002-09-19 | Centro De Investigaciones Energeticas Medioambientales Y Tecnologicas (C.I.E.M.A.T.) | Dermis artificial y metodo de obtencion |
WO2003002154A1 (es) * | 2001-06-29 | 2003-01-09 | Centro De Investigaciones Energeticas Medioambientales Y Tecnologicas (C.I.E.M.A.T.) | Piel artificial autologa secretora de leptina y metodo de obtencion |
WO2003035115A3 (en) * | 2001-10-26 | 2003-07-17 | Baxter Int | Fibrin membrane and methods for its preparation- application to artificial skin |
EP1545565A1 (en) * | 2002-09-06 | 2005-06-29 | DBF Pharmaceuticals, Inc. | Methods and compositions for tissue regeneration |
WO2006123004A1 (es) | 2005-05-16 | 2006-11-23 | Fundación Para La Investigación Biomédica Del Hospital Gregorio Marañon | Matriz artificial de gel de fibrina endotelizada superproductora de factores proangiogénicos |
WO2013016836A1 (es) * | 2011-08-03 | 2013-02-07 | Manuel Eduardo Young Anze | Preparación de gel de fibrina útil como sistema de implante. |
Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0485210A2 (en) * | 1990-11-08 | 1992-05-13 | Otogen Corporation | Fibrin/collagen membrane material for biomedical use |
EP0526550A1 (en) * | 1990-04-24 | 1993-02-10 | EISENBERG, Mark | Composite living skin equivalents |
US5326356A (en) * | 1990-06-01 | 1994-07-05 | Fidia S.P.A. | Biocompatible perforated membranes, processes for their preparation, their use as a support in the in vitro growth of epithelial cells, the artificial skin obtained in this manner, and its use in skin grafts |
WO1996033750A1 (en) * | 1995-04-27 | 1996-10-31 | Fidia Advanced Biopolymers S.R.L. | Artificial skin containing as support biocompatible materials based on hyaluronic acid derivatives |
-
1997
- 1997-07-04 ES ES9701533A patent/ES2132027B1/es not_active Expired - Fee Related
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0526550A1 (en) * | 1990-04-24 | 1993-02-10 | EISENBERG, Mark | Composite living skin equivalents |
US5326356A (en) * | 1990-06-01 | 1994-07-05 | Fidia S.P.A. | Biocompatible perforated membranes, processes for their preparation, their use as a support in the in vitro growth of epithelial cells, the artificial skin obtained in this manner, and its use in skin grafts |
EP0485210A2 (en) * | 1990-11-08 | 1992-05-13 | Otogen Corporation | Fibrin/collagen membrane material for biomedical use |
WO1996033750A1 (en) * | 1995-04-27 | 1996-10-31 | Fidia Advanced Biopolymers S.R.L. | Artificial skin containing as support biocompatible materials based on hyaluronic acid derivatives |
Non-Patent Citations (1)
Title |
---|
YOUNAI, S. et al.: "Modulation of collagen synthesis by transforming growth factor-beta in keloid and hypertrophic scar fibroblasts", Annals of Plastic Surgery (1994), Vol. 33 (2), paginas 148-155 * |
Cited By (20)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US7449333B2 (en) | 2000-09-01 | 2008-11-11 | Dfb Pharmaceuticals, Inc. | Methods and compositions for tissue regeneration |
ES2173812A1 (es) * | 2001-03-01 | 2002-10-16 | Ct Investig Energeticas Ciemat | Dermis artificial y metodo de obtencion. |
WO2002072800A1 (es) * | 2001-03-01 | 2002-09-19 | Centro De Investigaciones Energeticas Medioambientales Y Tecnologicas (C.I.E.M.A.T.) | Dermis artificial y metodo de obtencion |
US9168125B2 (en) | 2001-03-01 | 2015-10-27 | Centro De Investigaciones Energeticas Medioambientales Y Tecnologicas | Artificial dermis and method of preparation |
US7244552B2 (en) | 2001-03-01 | 2007-07-17 | Centro De Investigaciones Energeticas Medioambientales Y Tecnologicas (C.I.E.M.A.T.) | Artificial dermis and production method therefor |
EP2165678A1 (en) * | 2001-03-01 | 2010-03-24 | Centro De Investigaciones Energeticas Medioambientales Y Tecnologicas (C.I.E.M.A.T.) | Artificial dermis and production method therefor |
WO2003002154A1 (es) * | 2001-06-29 | 2003-01-09 | Centro De Investigaciones Energeticas Medioambientales Y Tecnologicas (C.I.E.M.A.T.) | Piel artificial autologa secretora de leptina y metodo de obtencion |
ES2184623A1 (es) * | 2001-06-29 | 2003-04-01 | Ct Investig Energeticas Ciemat | Piel artificial autologa secretora de leptina y metodo de obtencion. |
WO2003035115A3 (en) * | 2001-10-26 | 2003-07-17 | Baxter Int | Fibrin membrane and methods for its preparation- application to artificial skin |
EP1545565A1 (en) * | 2002-09-06 | 2005-06-29 | DBF Pharmaceuticals, Inc. | Methods and compositions for tissue regeneration |
EP1545565A4 (en) * | 2002-09-06 | 2008-04-23 | Dbf Pharmaceuticals Inc | METHODS AND COMPOSITIONS FOR TISSUE REGENERATION |
US7700351B2 (en) | 2002-09-06 | 2010-04-20 | Dfb Pharmaceuticals, Inc. | Methods and compositions for tissue regeneration |
US7879605B2 (en) | 2002-09-06 | 2011-02-01 | Dfb Technology Holdings, Llc | Methods and compositions for tissue regeneration |
EP2311469A3 (en) * | 2002-09-06 | 2011-06-08 | DFB Technology Holdings, LLC | Methods and compositions for tissue regeneration |
US8137965B2 (en) | 2002-09-06 | 2012-03-20 | Dfb Technology Holdings, Llc | Methods and compositions for tissue regeneration |
US8323638B2 (en) | 2002-09-06 | 2012-12-04 | Dfb Technology Holdings, Llc | Methods and compositions for tissue regeneration |
US8679475B2 (en) | 2002-09-06 | 2014-03-25 | Smith & Nephew, Inc. | Methods and compositions for tissue regeneration |
US9173906B2 (en) | 2002-09-06 | 2015-11-03 | Smith & Nephew, Inc. | Methods and compositions for tissue regeneration |
WO2006123004A1 (es) | 2005-05-16 | 2006-11-23 | Fundación Para La Investigación Biomédica Del Hospital Gregorio Marañon | Matriz artificial de gel de fibrina endotelizada superproductora de factores proangiogénicos |
WO2013016836A1 (es) * | 2011-08-03 | 2013-02-07 | Manuel Eduardo Young Anze | Preparación de gel de fibrina útil como sistema de implante. |
Also Published As
Publication number | Publication date |
---|---|
ES2132027B1 (es) | 2000-04-01 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
EC2A | Search report published |
Date of ref document: 19990801 Kind code of ref document: A1 Effective date: 19990801 |
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FD2A | Announcement of lapse in spain |
Effective date: 20180807 |