ES2168112T3 - Metodo de amplificacion de repeticiones terminales. - Google Patents
Metodo de amplificacion de repeticiones terminales.Info
- Publication number
- ES2168112T3 ES2168112T3 ES95940256T ES95940256T ES2168112T3 ES 2168112 T3 ES2168112 T3 ES 2168112T3 ES 95940256 T ES95940256 T ES 95940256T ES 95940256 T ES95940256 T ES 95940256T ES 2168112 T3 ES2168112 T3 ES 2168112T3
- Authority
- ES
- Spain
- Prior art keywords
- rna
- dna
- template
- sequence
- promoter
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6865—Promoter-based amplification, e.g. nucleic acid sequence amplification [NASBA], self-sustained sequence replication [3SR] or transcription-based amplification system [TAS]
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Amplifiers (AREA)
- Steroid Compounds (AREA)
- Seal Device For Vehicle (AREA)
- Polysaccharides And Polysaccharide Derivatives (AREA)
- Peptides Or Proteins (AREA)
- Electrotherapy Devices (AREA)
- Channel Selection Circuits, Automatic Tuning Circuits (AREA)
- Oscillators With Electromechanical Resonators (AREA)
Abstract
LA INVENCION SE REFIERE A UN PROCESO PARA AMPLIFICAR UNA SECUENCIA ESPECIFICA DE ACIDO NUCLEICO O SU COMPLEMENTO A UNA TEMPERATURA RELATIVAMENTE CONSTANTE Y SIN ADICION EN SERIE DE REACTIVOS. EL PROCESO REQUIERE AÑADIR A UN MEDIO DE REACCION UNICO UNA ARN POLIMERASA, UNA ADN POLIMERASA, UNA RIBONUCLEASA QUE HIDROLICE EL ARN DE UN HIBRIDO ARN-ADN SIN HIDROLIZAR ARN O ADN DE UNA O DOS HEBRAS, RIBONUCLEOSIDOS Y DESOXIRRIBONUCLEOSIDOS TRIFOSFATOS. EL PROCESO PROPORCIONA UNA PRIMERA PLANTILLA DE ARN EN EL MEDIO DE REACCION. ESTA PRIMERA PLANTILLA DE ARN CONTIENE UNA SECUENCIA COMPLEMENTARIA DE UNA SECUENCIA ESPECIFICA DE ACIDO NUCLEICO, LAS SECUENCIAS INVERTIDAS CORRESPONDIENTES AL PROMOTOR Y EL LUGAR DE INICIACION QUE SON REORGANIZADAS POR LA ARN POLIMERASA, Y UNA SECUENCIA 5 - TERMINAL QUE ES COMPLEMENTARIA DE AL MENOS LA SECUENCIA INVERTIDA DEL LUGAR DE INICIACION. ASI, LA PRIMERA PLANTILLA DE ARN TIENE UNA SECUENCIA REPETIDA INVERTIDA QUE PUEDE PLEGARSE PARA FORMAR UNA ESTRUCTURA DE BUCLE ENEL TERMINAL 5 . LA ADN POLIMERASA UTILIZA LA PRIMERA PLANTILLA DE ARN PARA SINTETIZAR UNA SEGUNDA PLANTILLA DE ADN QUE EN CONJUNTO FORMAN UN HIDRIDO ARN-ADN. LA SEGUNDA PLANTILLA DE ADN CONTIENE UNA SECUENCIA NORMAL DEL PROMOTOR Y DEL LUGAR DE INICIACION. LA RIBONUCLEASA HIDROLIZA EL ARN QUE CONTIENE EL HIBRIDO DE ARN-ADN, PERMITIENDO QUE LA SECUENCIA DE CEBADO EN EL TERMINAL 3 PUEDA HIBRIDARSE CON LA SECUENCIA NORMAL DEL LUGAR DE INICIACION EN LA SEGUNDA PLANTILLA DEL ADN. SEGUIDAMENTE, LA ADN POLIMERASA UTILIZA LA SEGUNDA PLANTILLA DE ADN PARA SINTETIZAR EL PROMOTOR AMPLIANDO LA SECUENCIA DE CEBADO DEL TERMINAL 3 ARCIALMENTE DE DOBLE HEBRA, TIENE UN PROMOTOR ORIENTADO HACIA EL EXTREMO DE LA ESTRUCTURA EN BUCLE. LA ARN POLIMERASA RECONOCE ENTONCES AL PROMOTOR Y TRANSCRIBE LA SEGUNDA PLANTILLA DE ADN, CREANDO ASI COPIAS DE LA PRIMERA PLANTILLA DE ARN. EL PROCESO A PARTIR DE AQUI MANTIENE LAS CONDICIONES DE REACCION DURANTE UN TIEMPO SUFICIENTE PARA CONSEGUIR LA AMPLIFICACION DESEADA DE LASECUENCIA ESPECIFICA DE ACIDO NUCLEICO O SU COMPLEMENTO. ESTA INVENCION INCLUYE UN JUEGO DE REACTIVOS.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US08/345,505 US5665545A (en) | 1994-11-28 | 1994-11-28 | Terminal repeat amplification method |
Publications (1)
Publication Number | Publication Date |
---|---|
ES2168112T3 true ES2168112T3 (es) | 2002-06-01 |
Family
ID=23355318
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES95940256T Expired - Lifetime ES2168112T3 (es) | 1994-11-28 | 1995-11-28 | Metodo de amplificacion de repeticiones terminales. |
Country Status (10)
Country | Link |
---|---|
US (1) | US5665545A (es) |
EP (1) | EP0796343B1 (es) |
JP (1) | JP3745774B2 (es) |
AT (1) | ATE206467T1 (es) |
AU (1) | AU711589B2 (es) |
CA (1) | CA2205563A1 (es) |
DE (1) | DE69523068T2 (es) |
ES (1) | ES2168112T3 (es) |
FI (1) | FI972238A (es) |
WO (1) | WO1996017079A1 (es) |
Families Citing this family (46)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB9620209D0 (en) * | 1996-09-27 | 1996-11-13 | Cemu Bioteknik Ab | Method of sequencing DNA |
GB9626815D0 (en) | 1996-12-23 | 1997-02-12 | Cemu Bioteknik Ab | Method of sequencing DNA |
CA2222769C (en) * | 1997-01-17 | 2001-06-12 | F. Hoffmann-La Roche Ag | Primers for the detection of hiv-1 |
US5928869A (en) * | 1997-05-30 | 1999-07-27 | Becton, Dickinson And Company | Detection of nucleic acids by fluorescence quenching |
US6743605B1 (en) * | 1998-06-24 | 2004-06-01 | Enzo Life Sciences, Inc. | Linear amplification of specific nucleic acid sequences |
ATE374833T1 (de) | 1998-11-09 | 2007-10-15 | Eiken Chemical | Verfahren zur synthese von nukleinsäuren |
US6864050B2 (en) * | 1999-07-30 | 2005-03-08 | Affymetrix, Inc. | Single-phase amplification of nucleic acids |
US6692918B2 (en) * | 1999-09-13 | 2004-02-17 | Nugen Technologies, Inc. | Methods and compositions for linear isothermal amplification of polynucleotide sequences |
NZ517121A (en) * | 1999-09-13 | 2004-05-28 | Nugen Technologies Inc | Methods and compositions for linear isothermal amplification of polynucleotide sequences |
US6686156B2 (en) * | 2000-06-26 | 2004-02-03 | Nugen Technologies, Inc. | Methods and compositions for transcription-based nucleic acid amplification |
US7846733B2 (en) | 2000-06-26 | 2010-12-07 | Nugen Technologies, Inc. | Methods and compositions for transcription-based nucleic acid amplification |
ATE475720T1 (de) | 2000-12-13 | 2010-08-15 | Nugen Technologies Inc | Methoden und zusammensetzungen zur generierung einer vielzahl von kopien von nukleinsäuresequenzen und methoden zur detektion derselben |
US7094536B2 (en) * | 2001-03-09 | 2006-08-22 | Nugen Technologies, Inc. | Methods and compositions for amplification of RNA sequences |
AU2002252279B2 (en) | 2001-03-09 | 2005-05-12 | Nugen Technologies, Inc. | Methods and compositions for amplification of RNA sequences |
US7838270B2 (en) * | 2001-05-22 | 2010-11-23 | The University Of Chicago | Target-dependent transcription using deletion mutants of N4 RNA polymerase |
US8137911B2 (en) * | 2001-05-22 | 2012-03-20 | Cellscript, Inc. | Preparation and use of single-stranded transcription substrates for synthesis of transcription products corresponding to target sequences |
US7432084B2 (en) * | 2001-08-31 | 2008-10-07 | Rosetta Inpharmatics Llc | Methods for preparing nucleic acid samples |
CA2478875A1 (en) * | 2002-03-11 | 2003-09-25 | Nugen Technologies, Inc. | Methods for generating double stranded dna comprising a 3' single stranded portion and uses of these complexes for recombination |
SE0202867D0 (sv) * | 2002-09-27 | 2002-09-27 | Pyrosequencing Ab | New sequencing method |
CN102268428A (zh) * | 2002-11-21 | 2011-12-07 | 震源技术公司 | 使用编码双链启动子一条链的引物的方法 |
EP1613778B1 (en) * | 2003-04-14 | 2014-11-26 | Nugen Technologies, Inc. | Global amplification using a randomly primed composite primer |
ATE494392T1 (de) * | 2004-09-21 | 2011-01-15 | Life Technologies Corp | Zweifarbige echtzeit/endpunkt-quantifizierung von mikro-rnas (mirnas) |
WO2006081222A2 (en) * | 2005-01-25 | 2006-08-03 | Compass Genetics, Llc. | Isothermal dna amplification |
US7939258B2 (en) | 2005-09-07 | 2011-05-10 | Nugen Technologies, Inc. | Nucleic acid amplification procedure using RNA and DNA composite primers |
US7501254B2 (en) | 2006-07-20 | 2009-03-10 | Ghc Technologies, Inc. | Methods and compositions for amplification and capture of nucleic acid sequences |
EP2173872B1 (en) | 2007-06-29 | 2014-04-02 | CellScript, Inc. | Copy dna and sense rna |
US8034568B2 (en) | 2008-02-12 | 2011-10-11 | Nugen Technologies, Inc. | Isothermal nucleic acid amplification methods and compositions |
WO2009117698A2 (en) | 2008-03-21 | 2009-09-24 | Nugen Technologies, Inc. | Methods of rna amplification in the presence of dna |
JP5481841B2 (ja) * | 2008-11-28 | 2014-04-23 | 東ソー株式会社 | サイトケラチン19mRNAの測定方法 |
EP3578205A1 (en) | 2010-08-06 | 2019-12-11 | ModernaTX, Inc. | A pharmaceutical formulation comprising engineered nucleic acids and medical use thereof |
CN104531812A (zh) | 2010-10-01 | 2015-04-22 | 现代治疗公司 | 设计核酸及其使用方法 |
DK2665833T3 (en) | 2011-01-17 | 2017-07-24 | Life Technologies Corp | WORKING PROCEDURE FOR DETECTING LIGANDS USING NUCLEIC ACIDS |
JP2014511687A (ja) | 2011-03-31 | 2014-05-19 | モデルナ セラピューティクス インコーポレイテッド | 工学操作された核酸の送達および製剤 |
US9464124B2 (en) | 2011-09-12 | 2016-10-11 | Moderna Therapeutics, Inc. | Engineered nucleic acids and methods of use thereof |
ES2911677T3 (es) | 2011-10-03 | 2022-05-20 | Modernatx Inc | Nucleósidos, nucleótidos y ácidos nucleicos modificados, y sus usos |
RS63244B1 (sr) | 2011-12-16 | 2022-06-30 | Modernatx Inc | Kompozicije modifikovane mrna |
US9572897B2 (en) | 2012-04-02 | 2017-02-21 | Modernatx, Inc. | Modified polynucleotides for the production of cytoplasmic and cytoskeletal proteins |
US9878056B2 (en) | 2012-04-02 | 2018-01-30 | Modernatx, Inc. | Modified polynucleotides for the production of cosmetic proteins and peptides |
US9283287B2 (en) | 2012-04-02 | 2016-03-15 | Moderna Therapeutics, Inc. | Modified polynucleotides for the production of nuclear proteins |
US20140010861A1 (en) | 2012-04-02 | 2014-01-09 | modeRNA Therapeutics | Modified polynucleotides for the production of proteins associated with human disease |
CN102943100B (zh) * | 2012-11-23 | 2014-04-16 | 郑州安图生物工程股份有限公司 | 高效RNA逆转录合成cDNA的方法 |
LT2922554T (lt) | 2012-11-26 | 2022-06-27 | Modernatx, Inc. | Terminaliai modifikuota rnr |
US8980864B2 (en) | 2013-03-15 | 2015-03-17 | Moderna Therapeutics, Inc. | Compositions and methods of altering cholesterol levels |
KR20160067219A (ko) | 2013-10-03 | 2016-06-13 | 모더나 세라퓨틱스, 인코포레이티드 | 저밀도 지단백질 수용체를 암호화하는 폴리뉴클레오타이드 |
CN107208162B (zh) * | 2015-02-26 | 2020-12-29 | 株式会社日立高新技术 | 核酸分子的构建方法 |
WO2021007233A1 (en) * | 2019-07-10 | 2021-01-14 | The Board Of Trustees Of The Leland Stanford Junior University | Rna replication using transcription polymerases |
Family Cites Families (21)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4683202A (en) * | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
US4683195A (en) * | 1986-01-30 | 1987-07-28 | Cetus Corporation | Process for amplifying, detecting, and/or-cloning nucleic acid sequences |
CA1340807C (en) * | 1988-02-24 | 1999-11-02 | Lawrence T. Malek | Nucleic acid amplification process |
US5130238A (en) * | 1988-06-24 | 1992-07-14 | Cangene Corporation | Enhanced nucleic acid amplification process |
JP2939485B2 (ja) * | 1988-09-08 | 1999-08-25 | ザ・ソーク・インスチュート・フォーバイオロジカル・スタディーズ | 複製rnaに基づく増幅/検出系 |
CA2020958C (en) * | 1989-07-11 | 2005-01-11 | Daniel L. Kacian | Nucleic acid sequence amplification methods |
NO904633L (no) * | 1989-11-09 | 1991-05-10 | Molecular Diagnostics Inc | Amplifikasjon av nukleinsyrer ved transkriberbar haarnaalsprobe. |
US5215899A (en) * | 1989-11-09 | 1993-06-01 | Miles Inc. | Nucleic acid amplification employing ligatable hairpin probe and transcription |
US5194370A (en) * | 1990-05-16 | 1993-03-16 | Life Technologies, Inc. | Promoter ligation activated transcription amplification of nucleic acid sequences |
NO174693C (no) * | 1990-05-22 | 1994-06-22 | Jacobsen Clas T | Fremgangsmåte ved anbringelse av brannbeskyttende belegg, såsom matter, på konstruksjoner |
WO1992018521A1 (en) * | 1991-04-10 | 1992-10-29 | Life Technologies, Inc. | Method for amplifying and altering an rna sequence |
US5169766A (en) * | 1991-06-14 | 1992-12-08 | Life Technologies, Inc. | Amplification of nucleic acid molecules |
ES2211878T3 (es) * | 1991-08-30 | 2004-07-16 | The University Of Medicine And Dentistry Of New Jersey | Sobre-expresion de moleculas de adnn monocatenario. |
DE4213029A1 (de) * | 1991-09-26 | 1993-04-01 | Boehringer Mannheim Gmbh | Verfahren zur spezifischen vervielfaeltigung von nukleins aeuresequenzen |
DE4132133A1 (de) * | 1991-09-26 | 1993-04-01 | Boehringer Mannheim Gmbh | Verfahren zur spezifischen herstellung von ribonukleinsaeuren |
US5411875A (en) * | 1991-11-01 | 1995-05-02 | University Of Iowa Research Foundation | Method for retrieval of unknown flanking DNA sequence |
US5278051A (en) * | 1991-12-12 | 1994-01-11 | New York University | Construction of geometrical objects from polynucleotides |
AU3729393A (en) * | 1992-02-20 | 1993-09-13 | State Of Oregon Acting By And Through The Oregon State Board Of Higher Education On Behalf Of Oregon State University, The | Boomerand DNA amplification |
WO1994003624A1 (en) * | 1992-08-04 | 1994-02-17 | Auerbach Jeffrey I | Methods for the isothermal amplification of nucleic acid molecules |
CA2101119A1 (en) * | 1992-08-17 | 1994-02-18 | George T. Walker | Exonuclease decontamination method |
IL105894A (en) * | 1993-06-03 | 1997-02-18 | Asher Nathan | Method for the detection of nucleic acid sequence by using a detection ensemble and an amplification ensemble |
-
1994
- 1994-11-28 US US08/345,505 patent/US5665545A/en not_active Expired - Fee Related
-
1995
- 1995-11-28 AU AU41767/96A patent/AU711589B2/en not_active Ceased
- 1995-11-28 JP JP51818496A patent/JP3745774B2/ja not_active Expired - Fee Related
- 1995-11-28 ES ES95940256T patent/ES2168112T3/es not_active Expired - Lifetime
- 1995-11-28 CA CA002205563A patent/CA2205563A1/en not_active Abandoned
- 1995-11-28 AT AT95940256T patent/ATE206467T1/de not_active IP Right Cessation
- 1995-11-28 DE DE69523068T patent/DE69523068T2/de not_active Expired - Fee Related
- 1995-11-28 WO PCT/EP1995/004694 patent/WO1996017079A1/en active IP Right Grant
- 1995-11-28 EP EP95940256A patent/EP0796343B1/en not_active Expired - Lifetime
-
1997
- 1997-05-27 FI FI972238A patent/FI972238A/fi unknown
Also Published As
Publication number | Publication date |
---|---|
JP3745774B2 (ja) | 2006-02-15 |
CA2205563A1 (en) | 1996-06-06 |
DE69523068T2 (de) | 2002-06-06 |
WO1996017079A1 (en) | 1996-06-06 |
EP0796343A1 (en) | 1997-09-24 |
FI972238A0 (fi) | 1997-05-27 |
EP0796343B1 (en) | 2001-10-04 |
AU711589B2 (en) | 1999-10-14 |
JPH10510161A (ja) | 1998-10-06 |
US5665545A (en) | 1997-09-09 |
ATE206467T1 (de) | 2001-10-15 |
FI972238A (fi) | 1997-05-27 |
DE69523068D1 (en) | 2001-11-08 |
AU4176796A (en) | 1996-06-19 |
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