ES2162630T3 - Metodos para el apantallamiento en solucion de sondas de oligonucleotidos marcadas por fluorescencia. - Google Patents

Metodos para el apantallamiento en solucion de sondas de oligonucleotidos marcadas por fluorescencia.

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Publication number
ES2162630T3
ES2162630T3 ES95117981T ES95117981T ES2162630T3 ES 2162630 T3 ES2162630 T3 ES 2162630T3 ES 95117981 T ES95117981 T ES 95117981T ES 95117981 T ES95117981 T ES 95117981T ES 2162630 T3 ES2162630 T3 ES 2162630T3
Authority
ES
Spain
Prior art keywords
methods
label
oligonucleotide
oligonucleotid
fluorescence
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES95117981T
Other languages
English (en)
Inventor
Teresa Kim Huff Picone
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
F Hoffmann La Roche AG
Original Assignee
F Hoffmann La Roche AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by F Hoffmann La Roche AG filed Critical F Hoffmann La Roche AG
Application granted granted Critical
Publication of ES2162630T3 publication Critical patent/ES2162630T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6823—Release of bound markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification

Abstract

LA PRESENTE INVENCION PROPORCIONA METODOS PARA LA DETECCION DE UN CAMBIO EN LA LONGITUD DE UN OLIGONUCLEOTIDO ETIQUETADO CON UNA ETIQUETA DE EMISION DE LUZ MEDIANTE LA MEDICION DE LA EMISION DE LUZ EN LA PRESENCIA DE UN COMPUESTO DE ENLACE DEL ADN QUE INTERACTUA CON LA ETIQUETA PARA MODIFICAR LA EMISION DE LUZ DE LA ETIQUETA, EN DONDE EL GRADO DE INTERACCION DEPENDE DE LA LONGITUD DEL OLIGONUCLEOTIDO. LOS METODOS SON APLICABLES EN GENERAL A ENSAYOS DE DETECCION DE LA SECUENCIA DE ACIDO NUCLEICO QUE UTILIZA UNA REACCION QUE DA COMO RESULTADO LA DESCOMPOSICION SELECTIVA DE SONDAS DE OLIGONUCLEOTIDOS HIBRIDOS, Y, EN PARTICULAR A ENSAYOS DE AMPLIFICACION/DETECCION EN DONDE LAS SONDAS HIBRIDAS SON DESCOMPUESTAS CONCOMITANTES CON LA EXTENSION DEL CEBADOR.
ES95117981T 1994-11-23 1995-11-15 Metodos para el apantallamiento en solucion de sondas de oligonucleotidos marcadas por fluorescencia. Expired - Lifetime ES2162630T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US08/347,657 US5571673A (en) 1994-11-23 1994-11-23 Methods for in-solution quenching of fluorescently labeled oligonucleotide probes

Publications (1)

Publication Number Publication Date
ES2162630T3 true ES2162630T3 (es) 2002-01-01

Family

ID=23364666

Family Applications (1)

Application Number Title Priority Date Filing Date
ES95117981T Expired - Lifetime ES2162630T3 (es) 1994-11-23 1995-11-15 Metodos para el apantallamiento en solucion de sondas de oligonucleotidos marcadas por fluorescencia.

Country Status (14)

Country Link
US (1) US5571673A (es)
EP (1) EP0713921B1 (es)
JP (1) JP3600333B2 (es)
KR (1) KR100431421B1 (es)
CN (1) CN1131320C (es)
AT (1) ATE204333T1 (es)
AU (1) AU700885B2 (es)
CA (1) CA2163388C (es)
DE (1) DE69522176T2 (es)
DK (1) DK0713921T3 (es)
ES (1) ES2162630T3 (es)
NZ (1) NZ280506A (es)
PT (1) PT713921E (es)
RU (1) RU2149186C1 (es)

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Also Published As

Publication number Publication date
AU3901095A (en) 1996-05-30
CN1133888A (zh) 1996-10-23
CA2163388C (en) 2008-01-15
DE69522176T2 (de) 2002-05-29
JPH08220092A (ja) 1996-08-30
CA2163388A1 (en) 1996-05-24
EP0713921A2 (en) 1996-05-29
KR960017858A (ko) 1996-06-17
PT713921E (pt) 2002-02-28
NZ280506A (en) 1997-07-27
ATE204333T1 (de) 2001-09-15
DE69522176D1 (de) 2001-09-20
KR100431421B1 (ko) 2004-12-03
CN1131320C (zh) 2003-12-17
AU700885B2 (en) 1999-01-14
JP3600333B2 (ja) 2004-12-15
US5571673A (en) 1996-11-05
EP0713921B1 (en) 2001-08-16
EP0713921A3 (en) 1996-09-11
RU2149186C1 (ru) 2000-05-20
DK0713921T3 (da) 2001-11-12

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