ES2161885T3 - Sintesis de proteinas mediante ligacion quimica natural. - Google Patents

Sintesis de proteinas mediante ligacion quimica natural.

Info

Publication number
ES2161885T3
ES2161885T3 ES95919029T ES95919029T ES2161885T3 ES 2161885 T3 ES2161885 T3 ES 2161885T3 ES 95919029 T ES95919029 T ES 95919029T ES 95919029 T ES95919029 T ES 95919029T ES 2161885 T3 ES2161885 T3 ES 2161885T3
Authority
ES
Spain
Prior art keywords
ligation
proteins
native
full length
chemical ligation
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES95919029T
Other languages
English (en)
Inventor
Stephen B H Kent
Tom W Muir
Philip E Dawson
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Scripps Research Institute
Original Assignee
Scripps Research Institute
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Scripps Research Institute filed Critical Scripps Research Institute
Application granted granted Critical
Publication of ES2161885T3 publication Critical patent/ES2161885T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/04—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length on carriers
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/02—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length in solution
    • C07K1/026—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length in solution by fragment condensation in solution

Abstract

SE PRODUCEN PROTEINAS DE TAMAÑO MODERADO, CON ESQUELETOS PEPTIDICOS NATIVOS, MEDIANTE UN METODO DE UNION QUIMICA NATIVA. LA UNION QUIMICA NATIVA EMPLEA UNA REACCION QUIMIOSELECTIVA DE DOS SEGMENTOS PEPTIDICOS NO PROTEGIDOS, PARA PRODUCIR UN INTERMEDIARIO TRANSITORIO UNIDO A TIOESTER. DICHO INTERMEDIARIO SUFRE, A CONTINUACION, ESPONTANEAMENTE UNA REORGANIZACION, PARA PROPORCIONAR EL PRODUCTO DE UNION DE LONGITUD COMPLETA, CON UN ENLACE PEPTIDICO NATIVO EN EL SITIO DE UNION. LOS PRODUCTOS DE UNION DE LONGITUD COMPLETA SON QUIMICAMENTE IDENTICOS A LAS PROTEINAS PRODUCIDAS MEDIANTE SINTESIS EN CELULAS LIBRES. DICHOS PRODUCTOS SE PUEDEN REPLEGAR Y/O OXIDAR, COMO LES SEA PERMITIDO, PARA FORMAR MOLECULAS PROTEINAS QUE CONTENGAN PUENTES DISULFURO NATIVOS. LA TECNICA DE UNION QUIMICA NATIVA SE PUEDE EMPLEAR PARA SINTETIZAR PROTEINAS EN SU LONGITUD TOTAL.
ES95919029T 1995-05-04 1995-05-04 Sintesis de proteinas mediante ligacion quimica natural. Expired - Lifetime ES2161885T3 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CA002218620A CA2218620C (en) 1995-05-04 1995-05-04 Synthesis of proteins by native chemical ligation
PCT/US1995/005668 WO1996034878A1 (en) 1995-05-04 1995-05-04 Synthesis of proteins by native chemical ligation

Publications (1)

Publication Number Publication Date
ES2161885T3 true ES2161885T3 (es) 2001-12-16

Family

ID=25679740

Family Applications (1)

Application Number Title Priority Date Filing Date
ES95919029T Expired - Lifetime ES2161885T3 (es) 1995-05-04 1995-05-04 Sintesis de proteinas mediante ligacion quimica natural.

Country Status (13)

Country Link
EP (1) EP0832096B1 (es)
JP (1) JP3599346B2 (es)
AT (1) ATE203248T1 (es)
AU (1) AU711451B2 (es)
CA (1) CA2218620C (es)
DE (1) DE69521815T2 (es)
DK (1) DK0832096T3 (es)
ES (1) ES2161885T3 (es)
GR (1) GR3036895T3 (es)
MX (1) MX9708500A (es)
PT (1) PT832096E (es)
SI (1) SI0832096T1 (es)
WO (1) WO1996034878A1 (es)

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US6168784B1 (en) 1997-09-03 2001-01-02 Gryphon Sciences N-terminal modifications of RANTES and methods of use
US6844161B2 (en) 1997-09-04 2005-01-18 Gryphon Therapeutics, Inc. Modular protein libraries and methods of preparation
US6875594B2 (en) * 1997-11-13 2005-04-05 The Rockefeller University Methods of ligating expressed proteins
WO2000012536A2 (en) 1998-08-31 2000-03-09 Gryphon Sciences Lipid matrix-assisted chemical ligation and synthesis of membrane polypeptides
AUPP589598A0 (en) 1998-09-14 1998-10-08 University Of Queensland, The Novel peptides
EP1165757A1 (en) 1999-03-05 2002-01-02 Maxygen, Inc. Encryption of traits using split gene sequences
US7192713B1 (en) 1999-05-18 2007-03-20 President And Fellows Of Harvard College Stabilized compounds having secondary structure motifs
US7482425B2 (en) 1999-08-26 2009-01-27 Amylin Pharmaceuticals, Inc. Compositions for lipid matrix-assisted chemical ligation
US6277957B1 (en) * 2000-03-23 2001-08-21 Derek Hudson Method for production of acylthio derivatives
WO2001098458A2 (en) * 2000-06-19 2001-12-27 Zyomyx, Inc. Methods for immobilizing polypeptides
JP2004507557A (ja) 2000-09-01 2004-03-11 グリフォン　セラピューティクス，インコーポレーテッド 求核物質に対して安定なチオエステル生成化合物、製造および使用の方法
JP2004515471A (ja) * 2000-09-08 2004-05-27 グリフォン セラピューティクス，インコーポレーテッド 合成赤血球産生刺激タンパク質
GB0113657D0 (en) * 2001-06-05 2001-07-25 Geneprot Inc Improved native chemical ligation with three or more components
GB0123262D0 (en) * 2001-09-27 2001-11-21 Adprotech Ltd Polymeric compounds
US6908963B2 (en) * 2001-10-09 2005-06-21 Nektar Therapeutics Al, Corporation Thioester polymer derivatives and method of modifying the N-terminus of a polypeptide therewith
WO2003070764A1 (en) * 2002-02-19 2003-08-28 Rmf Dictagene S.A. Method for producing interferon
AU2003303595A1 (en) 2002-12-30 2004-07-29 Gryphon Therapeutics, Inc. Water-soluble thioester and selenoester compounds and methods for making and using the same
DE10335584B4 (de) 2003-07-31 2006-06-29 Philipps-Universität Marburg Verfahren zur Herstellung zyklischer Moleküle
CA2538005A1 (en) 2003-09-05 2005-05-19 Sloan-Kettering Institute For Cancer Research Method for preparing polyfunctionalized peptides and/or proteins via native chemical ligation
CA2544223C (en) 2003-11-05 2017-03-07 Dana-Farber Cancer Institute, Inc. Stabilized alpha helical peptides and uses thereof
WO2007120614A2 (en) 2006-04-11 2007-10-25 Sloan-Kettering Institute For Cancer Research Homogeneous erythropoietin and other peptides and proteins, methods and intermediates for their preparation
US20100113342A1 (en) * 2006-06-20 2010-05-06 Yount Nannette I Antimicrobial kinocidin compositions and methods of use
CN105061577A (zh) 2006-12-14 2015-11-18 爱勒让治疗公司 双巯基大环化系统
PT2118123E (pt) 2007-01-31 2016-02-10 Harvard College Péptidos de p53 estabilizados e suas utilizações
EP2564862B1 (en) 2007-02-23 2017-08-02 Aileron Therapeutics, Inc. Substituted amino acids for preparing triazole linked macrocyclic peptides
KR20160061439A (ko) 2007-03-28 2016-05-31 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 스티칭된 폴리펩티드
US8809258B2 (en) 2007-07-31 2014-08-19 Glytech, Inc. Method for producing peptide
WO2010011313A2 (en) * 2008-07-23 2010-01-28 President And Fellows Of Harvard College Ligation of stapled polypeptides
PT2330114T (pt) 2008-08-19 2017-11-17 Glytech Inc Método de produção de glicoproteína e método de triagem
EP3492492A1 (en) 2008-09-22 2019-06-05 Aileron Therapeutics, Inc. Methods for preparing purified polypeptide compositions
EP2376100B1 (en) 2009-01-14 2017-10-25 Aileron Therapeutics, Inc. Peptidomimetic macrocycles
EP2391714B2 (en) * 2009-01-30 2019-07-24 Whitehead Institute for Biomedical Research Methods for ligation and uses thereof
TW201031749A (en) 2009-02-10 2010-09-01 Otsuka Chemical Co Ltd IgG-Fc fragment and method for manufacturing the same
SG176300A1 (en) 2009-06-26 2012-01-30 Otsuka Chemical Co Ltd Process for production of peptide thioester
JP2012532929A (ja) 2009-07-13 2012-12-20 プレジデント アンド フェロウズ オブ ハーバード カレッジ 二機能性のステープリングされたポリペプチドおよびそれらの使用
WO2011007747A1 (ja) 2009-07-16 2011-01-20 大塚化学株式会社 糖鎖付加ａｉｌｉｍ細胞外ドメイン及びその製造方法
JP2013505300A (ja) 2009-09-22 2013-02-14 エルロン・セラピューティクス・インコーポレイテッド ペプチド模倣大環状分子
FR2952058B1 (fr) 2009-10-29 2013-10-04 Centre Nat Rech Scient Procede de ligation native de polypeptides
CN108570097A (zh) 2010-08-13 2018-09-25 爱勒让治疗公司 拟肽大环化合物
WO2012040459A2 (en) 2010-09-22 2012-03-29 President And Fellows Of Harvard College Beta-catenin targeting peptides and uses thereof
FR2971509B1 (fr) 2011-02-16 2013-02-22 Centre Nat Rech Scient Procede de preparation de peptides par assemblage de multiples fragments peptidiques
CN103534276B (zh) 2011-03-10 2016-03-02 株式会社糖锁工学研究所 具有唾液酸糖链的糖肽的制造方法、用于该制造方法的唾液酸糖链加成氨基酸衍生物及该糖肽
WO2012174423A1 (en) 2011-06-17 2012-12-20 President And Fellows Of Harvard College Stabilized polypeptides as regulators of rab gtpase function
EP2762485B1 (en) 2011-09-26 2018-08-01 Glytech, Inc. Method for producing polypeptide fragment with high efficiency, which is suitable for ncl method
FR2981352B1 (fr) 2011-10-17 2015-07-03 Centre Nat Rech Scient Procede de synthese de proteines
AU2012326026B2 (en) 2011-10-18 2017-04-13 Aileron Therapeutics, Inc. Peptidomimetic macrocyles
NZ627528A (en) 2012-02-15 2016-05-27 Aileron Therapeutics Inc Peptidomimetic macrocycles
US8987414B2 (en) 2012-02-15 2015-03-24 Aileron Therapeutics, Inc. Triazole-crosslinked and thioether-crosslinked peptidomimetic macrocycles
FR2988392B1 (fr) 2012-03-21 2014-04-11 Centre Nat Rech Scient Procede de ligation native
PT2920197T (pt) 2012-09-26 2021-06-11 Harvard College Péptidos agrafados com bloqueio de prolina e suas utilizações
WO2014071241A1 (en) 2012-11-01 2014-05-08 Aileron Therapeutics, Inc. Disubstituted amino acids and methods of preparation and use thereof
SG10201700952XA (en) 2013-03-13 2017-04-27 Harvard College Stapled and stitched polypeptides and uses thereof
BR112015031277A2 (pt) 2013-06-14 2017-09-19 Harvard College Moduladores do receptor de insulina de polipeptídeos estabilizados, análogos de insulina, composições farmacêuticas compreendendo os referidos polipeptídeos e métodos de preparação e usos dos mesmos
CN111620940A (zh) 2014-05-21 2020-09-04 哈佛大学的校长及成员们 Ras抑制肽和其用途
WO2016049355A1 (en) 2014-09-24 2016-03-31 Aileron Therapeutics, Inc. Peptidomimetic macrocycles and formulations thereof
CA2961258A1 (en) 2014-09-24 2016-03-31 Aileron Therapeutics, Inc. Peptidomimetic macrocycles and uses thereof
JP2018516844A (ja) 2015-03-20 2018-06-28 エルロン・セラピューティクス・インコーポレイテッドＡｉｌｅｒｏｎ Ｔｈｅｒａｐｅｕｔｉｃｓ，Ｉｎｃ． ペプチド模倣大環状分子およびその使用
US10059741B2 (en) 2015-07-01 2018-08-28 Aileron Therapeutics, Inc. Peptidomimetic macrocycles
JP2018528217A (ja) 2015-09-10 2018-09-27 エルロン・セラピューティクス・インコーポレイテッドＡｉｌｅｒｏｎ Ｔｈｅｒａｐｅｕｔｉｃｓ，Ｉｎｃ． Ｍｃｌ−１のモジュレーターとしてのペプチド模倣大環状分子
JP7067798B2 (ja) * 2017-03-02 2022-05-16 株式会社糖鎖工学研究所 アミノ酸ポリマーの製造方法

Family Cites Families (2)

* Cited by examiner, † Cited by third party
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IT1228537B (it) * 1987-07-30 1991-06-20 Magis Farmaceutici Derivati della cisteina ad azione espettorante
ES2099699T3 (es) * 1989-05-02 1997-06-01 Abbott Lab Fijacion covalente de los miembros de union especificos a las fases solidas.

Also Published As

Publication number Publication date
EP0832096B1 (en) 2001-07-18
PT832096E (pt) 2002-01-30
GR3036895T3 (en) 2002-01-31
JPH11508874A (ja) 1999-08-03
DK0832096T3 (da) 2001-10-01
CA2218620A1 (en) 1996-11-07
JP3599346B2 (ja) 2004-12-08
EP0832096A1 (en) 1998-04-01
WO1996034878A1 (en) 1996-11-07
DE69521815D1 (de) 2001-08-23
AU711451B2 (en) 1999-10-14
AU2474095A (en) 1996-11-21
DE69521815T2 (de) 2002-04-04
MX9708500A (es) 1998-02-28
CA2218620C (en) 2007-02-27
EP0832096A4 (en) 1998-08-05
ATE203248T1 (de) 2001-08-15
SI0832096T1 (en) 2001-12-31

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