AU2474095A - Synthesis of proteins by native chemical ligation - Google Patents

Synthesis of proteins by native chemical ligation

Info

Publication number
AU2474095A
AU2474095A AU24740/95A AU2474095A AU2474095A AU 2474095 A AU2474095 A AU 2474095A AU 24740/95 A AU24740/95 A AU 24740/95A AU 2474095 A AU2474095 A AU 2474095A AU 2474095 A AU2474095 A AU 2474095A
Authority
AU
Australia
Prior art keywords
ligation
native
proteins
full length
native chemical
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
AU24740/95A
Other versions
AU711451B2 (en
Inventor
Philip E Dawson
Stephen B.H. Kent
Tom W Muir
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Scripps Research Institute
Original Assignee
Scripps Research Institute
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Scripps Research Institute filed Critical Scripps Research Institute
Publication of AU2474095A publication Critical patent/AU2474095A/en
Application granted granted Critical
Publication of AU711451B2 publication Critical patent/AU711451B2/en
Anticipated expiration legal-status Critical
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/04—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length on carriers
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/02—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length in solution
    • C07K1/026—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length in solution by fragment condensation in solution

Abstract

Proteins of moderate size having native peptide backbones are produced by a method of native chemical ligation. Native chemical ligation employs a chemoselective reaction of two unprotected peptide segments to produce a transient thioester-linked intermediate. The transient thioester-linked intermediate then spontaneously undergoes a rearrangement to provide the full length ligation product having a native peptide bond at the ligation site. Full length ligation products are chemically identical to proteins produced by cell free synthesis. Full length ligation products may be refolded and/or oxidized, as allowed, to form native disulfide-containing protein molecules. The technique of native chemical ligation is employable for chemically synthesizing full length proteins.
AU24740/95A 1995-05-04 1995-05-04 Synthesis of proteins by native chemical ligation Expired AU711451B2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
PCT/US1995/005668 WO1996034878A1 (en) 1995-05-04 1995-05-04 Synthesis of proteins by native chemical ligation
CA002218620A CA2218620C (en) 1995-05-04 1995-05-04 Synthesis of proteins by native chemical ligation

Publications (2)

Publication Number Publication Date
AU2474095A true AU2474095A (en) 1996-11-21
AU711451B2 AU711451B2 (en) 1999-10-14

Family

ID=25679740

Family Applications (1)

Application Number Title Priority Date Filing Date
AU24740/95A Expired AU711451B2 (en) 1995-05-04 1995-05-04 Synthesis of proteins by native chemical ligation

Country Status (13)

Country Link
EP (1) EP0832096B1 (en)
JP (1) JP3599346B2 (en)
AT (1) ATE203248T1 (en)
AU (1) AU711451B2 (en)
CA (1) CA2218620C (en)
DE (1) DE69521815T2 (en)
DK (1) DK0832096T3 (en)
ES (1) ES2161885T3 (en)
GR (1) GR3036895T3 (en)
MX (1) MX9708500A (en)
PT (1) PT832096E (en)
SI (1) SI0832096T1 (en)
WO (1) WO1996034878A1 (en)

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US6184344B1 (en) * 1995-05-04 2001-02-06 The Scripps Research Institute Synthesis of proteins by native chemical ligation
US6307018B1 (en) * 1996-12-24 2001-10-23 The Scripps Research Institute General chemical ligation
JP2001512306A (en) 1997-01-08 2001-08-21 ライフ テクノロジーズ，インコーポレイテッド Methods for producing proteins
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US6168784B1 (en) 1997-09-03 2001-01-02 Gryphon Sciences N-terminal modifications of RANTES and methods of use
AU750244B2 (en) 1997-09-04 2002-07-11 Gryphon Sciences Modular protein libraries and methods of preparation
US6875594B2 (en) * 1997-11-13 2005-04-05 The Rockefeller University Methods of ligating expressed proteins
KR20010085743A (en) 1998-08-31 2001-09-07 추후제출 Lipid matrix-assisted chemical ligation and synthesis of membrane polypeptides
AUPP589598A0 (en) 1998-09-14 1998-10-08 University Of Queensland, The Novel peptides
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AU2001269906A1 (en) * 2000-06-19 2002-01-02 Zyomyx, Inc. Methods for immobilizing polypeptides
JP2004507557A (en) * 2000-09-01 2004-03-11 グリフォン　セラピューティクス，インコーポレーテッド Nucleophilically stable thioester-forming compounds, methods of manufacture and use
RU2003109746A (en) * 2000-09-08 2005-01-27 Грифон Терапьютикс, Инк. (Us) SYNTHETIC PROTEINS STIMULATING ERYTHROPOESIS
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AU2003214068A1 (en) * 2002-02-19 2003-09-09 Rmf Dictagene S.A. Method for producing interferon
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DE10335584B4 (en) 2003-07-31 2006-06-29 Philipps-Universität Marburg Process for the preparation of cyclic molecules
US7635750B2 (en) 2003-09-05 2009-12-22 Sloan-Kettering Institute For Cancer Research Method for preparing polyfunctionalized peptides and/or proteins via native chemical ligation
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EP2010556B1 (en) 2006-04-11 2015-09-23 Sloan-Kettering Institute for Cancer Research Homogeneous erythropoietin and method for their preparation
EP2628749A3 (en) * 2006-06-20 2013-12-04 Los Angeles Biomedical Research Institute at Harbor-UCLA Medical Center Antimicrobial kinocidin compostions and methods of use
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WO2008104000A2 (en) 2007-02-23 2008-08-28 Aileron Therapeutics, Inc. Triazole macrocycle systems
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JP5517620B2 (en) 2007-07-31 2014-06-11 株式会社糖鎖工学研究所 Method for producing peptide
EP2356139A4 (en) 2008-07-23 2013-01-09 Harvard College Ligation of stapled polypeptides
BRPI0917298B8 (en) 2008-08-19 2021-05-25 Glytech Inc glycoprotein production method and glycoprotein tracking method
EP2342221B1 (en) 2008-09-22 2018-11-07 Aileron Therapeutics, Inc. Methods for preparing purified polypeptide compositions
WO2010083347A2 (en) 2009-01-14 2010-07-22 Aileron Therapeutics, Inc. Peptidomimetic macrocycles
WO2010087994A2 (en) 2009-01-30 2010-08-05 Whitehead Institute For Biomedical Research Methods for ligation and uses thereof
TW201031749A (en) 2009-02-10 2010-09-01 Otsuka Chemical Co Ltd IgG-Fc fragment and method for manufacturing the same
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AU2010273220B2 (en) 2009-07-13 2015-10-15 President And Fellows Of Harvard College Bifunctional stapled polypeptides and uses thereof
US20120114595A1 (en) 2009-07-16 2012-05-10 Otsuka Chemical Co., Ltd. Sugar chain-added ailim extracellular domain and method for producing same
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FR2981352B1 (en) 2011-10-17 2015-07-03 Centre Nat Rech Scient PROCESS FOR PROTEIN SYNTHESIS
CN108929375A (en) 2011-10-18 2018-12-04 爱勒让治疗公司 Peptidomimetic macrocyclic compound
US8927500B2 (en) 2012-02-15 2015-01-06 Aileron Therapeutics, Inc. Peptidomimetic macrocycles
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FR2988392B1 (en) 2012-03-21 2014-04-11 Centre Nat Rech Scient NATIVE LIGATION METHOD
HUE054444T2 (en) 2012-09-26 2021-09-28 Harvard College Proline-locked stapled peptides and uses thereof
JP6526563B2 (en) 2012-11-01 2019-06-05 エイルロン セラピューティクス，インコーポレイテッド Disubstituted amino acids and methods for their preparation and use
KR20150131244A (en) 2013-03-13 2015-11-24 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 Stapled and stitched polypeptides and uses thereof
CN105492460A (en) 2013-06-14 2016-04-13 哈佛大学的校长及成员们 Stabilized polypeptide insulin receptor modulators
CN106573032B (en) 2014-05-21 2022-03-18 哈佛大学的校长及成员们 RAS inhibitory peptides and uses thereof
US10905739B2 (en) 2014-09-24 2021-02-02 Aileron Therapeutics, Inc. Peptidomimetic macrocycles and formulations thereof
SG10201902594QA (en) 2014-09-24 2019-04-29 Aileron Therapeutics Inc Peptidomimetic macrocycles and uses thereof
MX2017011834A (en) 2015-03-20 2018-04-11 Aileron Therapeutics Inc Peptidomimetic macrocycles and uses thereof.
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AU2018228182B2 (en) 2017-03-02 2024-04-11 Glytech, Inc. Production method for amino-acid polymer

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IT1228537B (en) * 1987-07-30 1991-06-20 Magis Farmaceutici EXISTING ACTION CISTEIN DERIVATIVES
CA2015938C (en) * 1989-05-02 1999-09-07 Kevin M. Knigge Covalent attachment of specific binding members to a solid phase

Also Published As

Publication number Publication date
DK0832096T3 (en) 2001-10-01
PT832096E (en) 2002-01-30
CA2218620C (en) 2007-02-27
EP0832096A1 (en) 1998-04-01
CA2218620A1 (en) 1996-11-07
AU711451B2 (en) 1999-10-14
GR3036895T3 (en) 2002-01-31
EP0832096A4 (en) 1998-08-05
ES2161885T3 (en) 2001-12-16
EP0832096B1 (en) 2001-07-18
DE69521815T2 (en) 2002-04-04
JPH11508874A (en) 1999-08-03
MX9708500A (en) 1998-02-28
JP3599346B2 (en) 2004-12-08
WO1996034878A1 (en) 1996-11-07
SI0832096T1 (en) 2001-12-31
ATE203248T1 (en) 2001-08-15
DE69521815D1 (en) 2001-08-23

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