ES2152864A1 - Procedimiento para la fabricacion de l-carnitina a partir de crotonobetaina. - Google Patents

Procedimiento para la fabricacion de l-carnitina a partir de crotonobetaina.

Info

Publication number
ES2152864A1
ES2152864A1 ES009802324A ES9802324A ES2152864A1 ES 2152864 A1 ES2152864 A1 ES 2152864A1 ES 009802324 A ES009802324 A ES 009802324A ES 9802324 A ES9802324 A ES 9802324A ES 2152864 A1 ES2152864 A1 ES 2152864A1
Authority
ES
Spain
Prior art keywords
crotonobetaine
carnitine
producing
continuously operating
operating cell
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
ES009802324A
Other languages
English (en)
Other versions
ES2152864B1 (es
Inventor
Pastor Jose Luis Iborra
Diaz Manuel Canovas
De Castro Jose Maria Obon
Hans Peter Kleber
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Universidad de Murcia
Original Assignee
Universidad de Murcia
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Universidad de Murcia filed Critical Universidad de Murcia
Publication of ES2152864A1 publication Critical patent/ES2152864A1/es
Application granted granted Critical
Publication of ES2152864B1 publication Critical patent/ES2152864B1/es
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/007—Carnitine; Butyrobetaine; Crotonobetaine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P1/00—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/822—Microorganisms using bacteria or actinomycetales
    • Y10S435/848—Escherichia
    • Y10S435/849—Escherichia coli

Landscapes

  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Mycology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
  • Separation Using Semi-Permeable Membranes (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

Procedimiento para la fabricación de L-carnitina a partir de crotonobetaína. Consiste en la obtención de una manera económicamente ventajosa, de L-carnitina a partir de crotonobetaína, sales de crotonobetaína o derivados a través de células inmovilizadas de Escherichia coli 044 K74 en un reactor de reciclado de células que funciona de forma continua. Las células en crecimiento o en reposo de E. coli son retenidas en un reactor de reciclado de células que funciona de forma continua, por medio de membranas de micro o ultrafiltración, que están dispuestas como módulo de membrana plana o de fibras huecas. La L-carnitina tiene numerosas aplicaciones clínicas, entre las que destacan la profilaxis y tratamiento de las enfermedades cardíacas.
ES009802324A 1997-11-08 1998-11-05 Procedimiento para la fabricacion de l-carnitina a partir de crotonobetaina. Expired - Fee Related ES2152864B1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE19749480A DE19749480A1 (de) 1997-11-08 1997-11-08 Verfahren zur Herstellung von L-Carnitin aus Crotonobetain

Publications (2)

Publication Number Publication Date
ES2152864A1 true ES2152864A1 (es) 2001-02-01
ES2152864B1 ES2152864B1 (es) 2001-09-01

Family

ID=7848083

Family Applications (1)

Application Number Title Priority Date Filing Date
ES009802324A Expired - Fee Related ES2152864B1 (es) 1997-11-08 1998-11-05 Procedimiento para la fabricacion de l-carnitina a partir de crotonobetaina.

Country Status (15)

Country Link
US (1) US6653112B2 (es)
EP (1) EP1049794B1 (es)
JP (1) JP2001521760A (es)
KR (1) KR100575297B1 (es)
CN (1) CN1163615C (es)
AT (1) ATE236989T1 (es)
AU (1) AU743520B2 (es)
BR (1) BR9813990A (es)
CA (1) CA2314328A1 (es)
DE (2) DE19749480A1 (es)
DK (1) DK1049794T3 (es)
ES (1) ES2152864B1 (es)
HK (1) HK1031237A1 (es)
PT (1) PT1049794E (es)
WO (1) WO1999024597A1 (es)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2220219A1 (es) * 2003-05-23 2004-12-01 Universidad De Murcia Procedimiento para la produccion de l-carnitina a partir de crotonobetaina, d-carnitina, sus sales y derivados, mediante celulas permeabilizadas de proteus sp. o escherichia coli.

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7407778B2 (en) * 2002-02-07 2008-08-05 Pettegrew Jay W Compounds, compositions and methods for treating neuropsychiatric disorders
US7700074B2 (en) * 2002-02-07 2010-04-20 Pettegrew Jay W Method and system for diagnosis of neuropsychiatric disorders including chronic alcoholism
EP1641806B1 (en) * 2003-05-29 2009-07-29 Jay W. Pettegrew Glycerophosphocholine and its derivatives for medical imaging of neuropsychiatric disorders
US20060257842A1 (en) * 2003-05-29 2006-11-16 Pettegrew Jay W Cryopreservation media and molecules
FR3025213A1 (fr) * 2014-08-28 2016-03-04 Algobiotech Procede d'induction de la synthese des phycobiliproteines
CN104928225B (zh) * 2015-05-27 2018-06-12 武汉中科光谷绿色生物技术有限公司 一种产l-肉碱的大肠杆菌基因工程菌及构建方法和应用

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0320460A2 (en) * 1987-10-26 1989-06-14 Sigma-Tau Industrie Farmaceutiche Riunite S.p.A. Process for isolating the enzyme carnitinehydrolyase from strains belonging to the family enterobacteriaceae and use of the immobilized enzyme for producing L(-)-carnitine

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DD221905B1 (de) * 1983-11-03 1987-03-18 Univ Leipzig Verfahren zur herstellung von l(-)-carnitin und seinen derivaten
FI86889C (fi) * 1984-03-29 1992-10-26 Lonza Ag Foerfarande foer framstaellning av av l-karnitin pao mikrobiologiskt saett
CH664374A5 (de) * 1985-02-27 1988-02-29 Lonza Ag Verfahren zur herstellung von l-carnitin auf mikrobiologischem weg.
US4978616A (en) * 1985-02-28 1990-12-18 Verax Corporation Fluidized cell cultivation process
DD281735A7 (de) * 1987-10-26 1990-08-22 Univ Leipzig Verfahren zur enzymatischen synthese von l(-)-carnitin

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0320460A2 (en) * 1987-10-26 1989-06-14 Sigma-Tau Industrie Farmaceutiche Riunite S.p.A. Process for isolating the enzyme carnitinehydrolyase from strains belonging to the family enterobacteriaceae and use of the immobilized enzyme for producing L(-)-carnitine

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
CHEMICAL ABSTRACTS, Vol. 115, no 1, 08.07.1991 (Columbus, OHIO, US) Resumen no 6956. JUNG, H. et al.: "Production of L(-)-carnitine by biotransformation" XP002096268 DECHEMA BIOTECHNOL. CONF. (1990), 4 (PT. B, LECT. DECHEMA ANNU. MEET. BIOTECHNOL. 8th, 1990), 1041-4 CODEN: DBCOEU *
CHEMICAL ABSTRACTS, Vol. 117, no 19, 08.11.1992 (Columbus, OHIO, US) Resumen no 187989. JUNG, H. et al.: "L-(-)-Carnitine synthesis by stereoselective hydratation of crotonobetaine" XP002096269. PROC. -EUR. CONGR. BIOTECHNOL., 5TH (1990), Volumen 1, 251-4. EDITOR(S): CHRISTIANSEN C.; MUNCK, L.; VILLADSEN, J., PUBLISHER: MUNKSGAARD, COPENHAGEN, DEN. CODEN: 57RVAO *
CHEMICAL ABSTRACTS, Vol. 128, no 2, 12.01.1998 (Columbus, OHIO, US) Resumen no 12603. OBON, JOSE MARIA et al.: L(-)-Carnitine production with immobilized Escherichi coli cells in continuous reactors" XP002096270. ENZYME MICROB. TECHNOL. (1997), 21 (7), 531-536. CODEN: EMTED2; ISSN: 0141-0229 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2220219A1 (es) * 2003-05-23 2004-12-01 Universidad De Murcia Procedimiento para la produccion de l-carnitina a partir de crotonobetaina, d-carnitina, sus sales y derivados, mediante celulas permeabilizadas de proteus sp. o escherichia coli.
WO2004104207A1 (es) * 2003-05-23 2004-12-02 Universidad De Murcia Procedimiento para la producción de l-carnitina a partir de crotonobetaína, d-carnitina, sus sales y derivados, mediante células permeabilizadas de proteus sp. o escherichia coli

Also Published As

Publication number Publication date
JP2001521760A (ja) 2001-11-13
US6653112B2 (en) 2003-11-25
CN1278303A (zh) 2000-12-27
BR9813990A (pt) 2000-09-26
WO1999024597A1 (de) 1999-05-20
EP1049794B1 (de) 2003-04-09
EP1049794A1 (de) 2000-11-08
DE19749480A1 (de) 1999-05-20
US20030073203A1 (en) 2003-04-17
AU2243699A (en) 1999-05-31
DE59807901D1 (de) 2003-05-15
AU743520B2 (en) 2002-01-31
DK1049794T3 (da) 2003-07-28
HK1031237A1 (en) 2001-06-08
ES2152864B1 (es) 2001-09-01
CN1163615C (zh) 2004-08-25
CA2314328A1 (en) 1999-05-20
KR20010031894A (ko) 2001-04-16
PT1049794E (pt) 2003-08-29
ATE236989T1 (de) 2003-04-15
KR100575297B1 (ko) 2006-05-02

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