CA2340712A1 - Method and device for carrying out biochemical reactions with a high throughput - Google Patents

Method and device for carrying out biochemical reactions with a high throughput Download PDF

Info

Publication number
CA2340712A1
CA2340712A1 CA002340712A CA2340712A CA2340712A1 CA 2340712 A1 CA2340712 A1 CA 2340712A1 CA 002340712 A CA002340712 A CA 002340712A CA 2340712 A CA2340712 A CA 2340712A CA 2340712 A1 CA2340712 A1 CA 2340712A1
Authority
CA
Canada
Prior art keywords
wells
inner housing
supply chamber
carrying
supply
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002340712A
Other languages
French (fr)
Other versions
CA2340712C (en
Inventor
Thomas Metzler
Hans Schels
Rolf Reichhuber
Jochen Kluge
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Roche Diagnostics GmbH
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2340712A1 publication Critical patent/CA2340712A1/en
Application granted granted Critical
Publication of CA2340712C publication Critical patent/CA2340712C/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J19/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J19/24—Stationary reactors without moving elements inside
    • B01J19/2475—Membrane reactors
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J19/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J19/0046—Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/12—Well or multiwell plates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/34—Internal compartments or partitions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/38—Caps; Covers; Plugs; Pouring means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00049—Controlling or regulating processes
    • B01J2219/00051—Controlling the temperature
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00279—Features relating to reactor vessels
    • B01J2219/00281—Individual reactor vessels
    • B01J2219/00286—Reactor vessels with top and bottom openings
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00279—Features relating to reactor vessels
    • B01J2219/00306—Reactor vessels in a multiple arrangement
    • B01J2219/00313—Reactor vessels in a multiple arrangement the reactor vessels being formed by arrays of wells in blocks
    • B01J2219/00315—Microtiter plates
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00351—Means for dispensing and evacuation of reagents
    • B01J2219/00423—Means for dispensing and evacuation of reagents using filtration, e.g. through porous frits
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00479—Means for mixing reactants or products in the reaction vessels
    • B01J2219/00481—Means for mixing reactants or products in the reaction vessels by the use of moving stirrers within the reaction vessels
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00479—Means for mixing reactants or products in the reaction vessels
    • B01J2219/00484—Means for mixing reactants or products in the reaction vessels by shaking, vibrating or oscillating of the reaction vessels
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00495—Means for heating or cooling the reaction vessels
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00585—Parallel processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00718—Type of compounds synthesised
    • B01J2219/0072—Organic compounds
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B60/00—Apparatus specially adapted for use in combinatorial chemistry or with libraries
    • C40B60/14—Apparatus specially adapted for use in combinatorial chemistry or with libraries for creating libraries

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Sustainable Development (AREA)
  • Biomedical Technology (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Thermal Sciences (AREA)
  • Physics & Mathematics (AREA)
  • General Chemical & Material Sciences (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Physical Or Chemical Processes And Apparatus (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Peptides Or Proteins (AREA)
  • Separation Using Semi-Permeable Membranes (AREA)

Abstract

Device, method and kits for carrying out biochemical reactions, in particular for cell-free polypeptide biosynthesis composed of an external housing which encloses an inner housing with incorporated wells and a supply chamber, wherein the wells of the inner housing each contain a producing system during the biochemical reaction, the supply chamber contains a supply liquid during the biochemical reaction and the wells of the inner housing and the supply chamber are separated by a semipermeable membrane characterized in that the inner housing has at least two wells the lower ends of which are closed by a semipermeable membrane and the upper ends of which protrude from the supply liquid contained in the supply chamber, and is connected to means for moving and incubating the producing systems and to the supply liquid.
CA002340712A 1999-06-18 2000-06-10 Method and device for carrying out biochemical reactions with a high throughput Expired - Fee Related CA2340712C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP99111762A EP1061128B1 (en) 1999-06-18 1999-06-18 Method and device for carrying out high throughput biochemical reactions
EP99111762.3 1999-06-18
PCT/EP2000/005378 WO2000078444A2 (en) 1999-06-18 2000-06-10 Method and device for carrying out biochemical reactions with a high throughput

Publications (2)

Publication Number Publication Date
CA2340712A1 true CA2340712A1 (en) 2000-12-28
CA2340712C CA2340712C (en) 2005-05-10

Family

ID=8238383

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002340712A Expired - Fee Related CA2340712C (en) 1999-06-18 2000-06-10 Method and device for carrying out biochemical reactions with a high throughput

Country Status (7)

Country Link
EP (2) EP1061128B1 (en)
JP (1) JP3650752B2 (en)
AT (2) ATE279511T1 (en)
CA (1) CA2340712C (en)
DE (2) DE59910840D1 (en)
ES (2) ES2229587T3 (en)
WO (1) WO2000078444A2 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2475401A (en) * 2009-11-11 2011-05-18 Ashe Morris Ltd Agitated cell reactors

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001080998A1 (en) * 2000-04-21 2001-11-01 University Of Rochester Biphasic reaction vessel and methods of use
DE102005013608A1 (en) * 2005-03-24 2006-09-28 Westfälische Wilhelms-Universität Münster Apparatus and method for cell-free analytical and preparative protein synthesis
RU2615446C2 (en) * 2012-11-30 2017-04-04 Байонир Корпорейшн Devices for automatic cell-free production of proteins and methods for proteins production using this product
US11421226B2 (en) * 2018-10-17 2022-08-23 Ramot At Tel-Aviv University Ltd. Programmable artificial cell
EP3725880A1 (en) * 2019-04-15 2020-10-21 ETH Zurich Method and kit for the purification of functional risc-associated small rnas
WO2023106291A1 (en) * 2021-12-07 2023-06-15 国立大学法人東京工業大学 Production of protein crystal material

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SE8102316L (en) * 1981-04-10 1982-10-11 Pharmacia Diagnostics Ab DEVICE FOR PERFORMANCE OF ANALYSIS
DE8717464U1 (en) * 1987-07-11 1988-12-29 Boehringer Ingelheim KG, 6507 Ingelheim Device for the simultaneous synthesis of several polypeptides
US5362624A (en) * 1988-05-25 1994-11-08 Boehringer Mannheim Gmbh Process for the determination of an immunologically detectable substance and a suitable reaction vessel therefor
US5478730A (en) * 1988-12-21 1995-12-26 Institute Of Protein Research Method of preparing polypeptides in cell-free translation system
DE4237113B4 (en) * 1992-11-03 2006-10-12 "Iba Gmbh" Peptides and their fusion proteins, expression vector and method of producing a fusion protein
US5462874A (en) * 1993-06-23 1995-10-31 Wolf; Martin L. Dialyzed multiple well tissue culture plate
KR0131166B1 (en) * 1994-05-04 1998-04-11 최차용 Preparation process of proteins in a non-cell system
JP3686917B2 (en) * 1996-06-05 2005-08-24 独立行政法人理化学研究所 Incubator for trace samples
ATE258592T1 (en) * 1997-07-31 2004-02-15 Roche Diagnostics Gmbh METHOD AND DEVICE FOR CARRYING OUT BIOCHEMICAL REACTIONS
RU2148649C1 (en) * 1998-03-31 2000-05-10 Институт белка РАН Method and apparatus for preparing polypeptides in acellular system (versions)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2475401A (en) * 2009-11-11 2011-05-18 Ashe Morris Ltd Agitated cell reactors
GB2475401B (en) * 2009-11-11 2015-03-18 Ashe Morris Ltd Improved agitated cell reactor

Also Published As

Publication number Publication date
WO2000078444A3 (en) 2001-04-26
EP1126912A1 (en) 2001-08-29
ES2260016T3 (en) 2006-11-01
ATE322336T1 (en) 2006-04-15
EP1061128A1 (en) 2000-12-20
JP3650752B2 (en) 2005-05-25
DE59910840D1 (en) 2004-11-18
CA2340712C (en) 2005-05-10
JP2003502146A (en) 2003-01-21
ATE279511T1 (en) 2004-10-15
DE50012526D1 (en) 2006-05-18
EP1126912B1 (en) 2006-04-05
ES2229587T3 (en) 2005-04-16
WO2000078444A2 (en) 2000-12-28
EP1061128B1 (en) 2004-10-13

Similar Documents

Publication Publication Date Title
ATE234930T1 (en) ENZYMATIC PRODUCTION OF HOMOGENTISCIC ACID
EP1333517A3 (en) Fuel cell assembly and fuel cell system thereof
GB2368032A (en) Low volume chemical and biochemical reaction system
AU2195699A (en) Mixing method
EP0985446A3 (en) Two stage reactor for continuous three phase slurry hydrogenation and method of operation
CA2445914A1 (en) Assay system
WO1999030154A3 (en) Continuous format high throughput screening
WO2000013018A3 (en) Support for a method for determining analytes and a method for producing the support
WO2001045869A3 (en) Microporous diffuser
EP0770869A3 (en) Gas chromatographic system with controlled sample transfer
DK0859661T3 (en) Apparatus and method for multiple chemical reactions
AU6421399A (en) Interpretive language architecture for controlling the attributes of a physical,chemical, or thermodynamic process
MX9700818A (en) Bacterial production of hydrophobic polypeptides.
ATE85239T1 (en) APPARATUS FOR CHEMICAL GENERATING AND SAMPLING OF GASES.
WO2002066596A3 (en) Device and method for analyzing ion channels in membranes
CA2340712A1 (en) Method and device for carrying out biochemical reactions with a high throughput
DE50002990D1 (en) DEVICE FOR CELL-FREE SYNTHESIS OF PROTEINS
WO2003012082A3 (en) Method for modulating stem cell differentiation using stem loop rna
WO1999045143A3 (en) A fluorescence polarization screening method
CA2102622A1 (en) Method of Making Alkylaluminoxane
DK0785986T3 (en) Process for producing hydrophobic products and suitable device for this purpose
WO2002102504A3 (en) The production of molecular arrays
AU2001289614A1 (en) Microreactor device for solid-phase supported synthesis and microreactor system comprising individual microreactor devices
DE3873518D1 (en) DEVICE FOR CARRYING OUT MICROBIOLOGICAL TESTS.
ES2152864A1 (en) Method for producing l-carnitine from crotonobetaine

Legal Events

Date Code Title Description
EEER Examination request
MKLA Lapsed

Effective date: 20170612