ES2120946T3 - Sistemas y medios de cultivo celular. - Google Patents
Sistemas y medios de cultivo celular.Info
- Publication number
- ES2120946T3 ES2120946T3 ES90306084T ES90306084T ES2120946T3 ES 2120946 T3 ES2120946 T3 ES 2120946T3 ES 90306084 T ES90306084 T ES 90306084T ES 90306084 T ES90306084 T ES 90306084T ES 2120946 T3 ES2120946 T3 ES 2120946T3
- Authority
- ES
- Spain
- Prior art keywords
- cells
- population
- present
- culture systems
- cellular culture
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0625—Epidermal cells, skin cells; Cells of the oral mucosa
- C12N5/0629—Keratinocytes; Whole skin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
- A61L27/60—Materials for use in artificial skin
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/0068—General culture methods using substrates
- C12N5/0075—General culture methods using substrates using microcarriers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/05—Inorganic components
- C12N2500/10—Metals; Metal chelators
- C12N2500/12—Light metals, i.e. alkali, alkaline earth, Be, Al, Mg
- C12N2500/14—Calcium; Ca chelators; Calcitonin
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/05—Inorganic components
- C12N2500/10—Metals; Metal chelators
- C12N2500/20—Transition metals
- C12N2500/24—Iron; Fe chelators; Transferrin
- C12N2500/25—Insulin-transferrin; Insulin-transferrin-selenium
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/90—Serum-free medium, which may still contain naturally-sourced components
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/01—Modulators of cAMP or cGMP, e.g. non-hydrolysable analogs, phosphodiesterase inhibitors, cholera toxin
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/10—Growth factors
- C12N2501/11—Epidermal growth factor [EGF]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/30—Hormones
- C12N2501/38—Hormones with nuclear receptors
- C12N2501/39—Steroid hormones
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/30—Hormones
- C12N2501/38—Hormones with nuclear receptors
- C12N2501/39—Steroid hormones
- C12N2501/392—Sexual steroids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/30—Hormones
- C12N2501/38—Hormones with nuclear receptors
- C12N2501/395—Thyroid hormones
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2533/00—Supports or coatings for cell culture, characterised by material
- C12N2533/50—Proteins
- C12N2533/54—Collagen; Gelatin
Landscapes
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Biomedical Technology (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- General Health & Medical Sciences (AREA)
- Cell Biology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Dermatology (AREA)
- Transplantation (AREA)
- Oral & Maxillofacial Surgery (AREA)
- Medicinal Chemistry (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
EL PRESENTE INVENTO PROVEE SISTEMAS, METODOS Y MEDIOS DEFINIDOS QUIMICAMENTE PRA EL CULTIVO DE CELULAS, PARTICULARMENTE CELULAS EPITELIALES. LAS CELULAS DEBEN SER CULTIVADAS CON UNA CONCENTRACION DE CALCIO VARIADA. ADEMAS, UNA CONCENTRACION DE CALCIO EN EXCESO DE 1.00 MM DEBE SER USADA EN LA PRACTICA DEL PRESENTE INVENTO, SIN PERDIDA DE UNA POBLACION DE CELULAS PROLIFERATIVAS, Y MANTENIENDO ALTAS EFICIENCIAS DE FORMACION DE COLONIAS. LOS TIEMPOS DE DUPLICACION DE POBLACION SON DEL RANGO DE 16 A 33 HORAS. LAS CELULAS DEBEN SER CULTIVADAS EN SERIE PARA REALIZAR DE 20 A 50 DUPLICACIONES DE POBLACION.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US36104189A | 1989-06-05 | 1989-06-05 | |
US53225790A | 1990-06-01 | 1990-06-01 |
Publications (1)
Publication Number | Publication Date |
---|---|
ES2120946T3 true ES2120946T3 (es) | 1998-11-16 |
Family
ID=27001138
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES90306084T Expired - Lifetime ES2120946T3 (es) | 1989-06-05 | 1990-06-05 | Sistemas y medios de cultivo celular. |
Country Status (9)
Country | Link |
---|---|
US (1) | US5712163A (es) |
EP (1) | EP0403139B1 (es) |
JP (1) | JP2577114B2 (es) |
AT (1) | ATE169670T1 (es) |
CA (1) | CA2018228C (es) |
DE (1) | DE69032549T2 (es) |
DK (1) | DK0403139T3 (es) |
ES (1) | ES2120946T3 (es) |
IL (1) | IL94611A (es) |
Families Citing this family (71)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6585969B1 (en) | 1991-11-20 | 2003-07-01 | N. V. Innogenetics S.A. | Non-viable keratinocyte cell composition or lysate for promoting wound healing |
ES2149779T3 (es) * | 1991-11-20 | 2000-11-16 | Innogenetics Nv | Lisados derivados de queratinocitos para utilizacion como substancias cicatrizantes. |
US5610007A (en) * | 1993-01-21 | 1997-03-11 | Universite Laval | Chimeric sheets of epithelial cells |
US6326194B1 (en) | 1993-03-11 | 2001-12-04 | Geoffrey Michael Curtin | Method for providing cell growth |
CA2119064A1 (en) * | 1993-03-17 | 1994-09-18 | Richard A. Berg | Dermal-epidermal in vitro test system |
EP0754017B1 (en) * | 1994-04-29 | 2002-06-19 | SciMed Life Systems, Inc. | Stent with collagen |
DE4438015A1 (de) * | 1994-10-25 | 1996-05-02 | Boehringer Mannheim Gmbh | Epithelzellenhaltiges Biomaterial und dessen Verwendung als Transplantat |
JP3660026B2 (ja) * | 1995-09-04 | 2005-06-15 | 扶桑薬品工業株式会社 | 体外受精用培地組成物 |
FR2747395B1 (fr) * | 1996-04-16 | 1998-06-26 | Univ Nantes | Procede de primoculture in vitro de cellules epitheliales gastro-intestinales, composition cellulaire obtenue notamment par la mise en oeuvre du procede et applications de ladite composition en tant que systeme de production |
FR2757536B1 (fr) * | 1996-12-24 | 1999-03-05 | Univ Nantes | Procede et milieu de culture de cellules, composition cellul aire obtenue et ses applications en tant que systeme de production et de modele d'etude |
AU2642897A (en) * | 1996-04-16 | 1997-11-07 | Universite De Nantes | Cell culture process and medium, cellular composition obtained and its application as production system and study model |
EP0953041A4 (en) | 1996-08-30 | 2003-01-29 | Life Technologies Inc | SERUM-FREE CULTURAL MEDIUM FOR MAMMAL CELLS AND THEIR USE |
US6692961B1 (en) * | 1996-10-11 | 2004-02-17 | Invitrogen Corporation | Defined systems for epithelial cell culture and use thereof |
US5972332A (en) * | 1997-04-16 | 1999-10-26 | The Regents Of The University Of Michigan | Wound treatment with keratinocytes on a solid support enclosed in a porous material |
US6498138B1 (en) | 1998-03-11 | 2002-12-24 | University Of Southern California | Method of promoting production of living tissue equivalents |
ES2402739T3 (es) * | 1998-11-19 | 2013-05-08 | Organogenesis Inc. | Constructos tisulares modificados por bioingeniería y métodos para producirlos y utilizarlos |
US6248725B1 (en) * | 1999-02-23 | 2001-06-19 | Amgen, Inc. | Combinations and methods for promoting in vivo liver cell proliferation and enhancing in vivo liver-directed gene transduction |
US7056503B2 (en) * | 1999-08-19 | 2006-06-06 | Regents Of The University Of Michigan | Enclosures housing cell-coated supports for treating tumors |
AU7067200A (en) * | 1999-08-23 | 2001-03-19 | Organogenesis Inc. | Skin care compositions and treatments |
FR2812004B1 (fr) * | 2000-07-24 | 2002-12-27 | Ccd Lab | Milieux de culture pour fecondation in vitro, ou pour la culture de follicules, cellules germinales males ou embryons |
NZ528166A (en) * | 2001-03-02 | 2007-05-31 | Stratatech Corp | A method for preparing a seeded dermal equivalent |
US6506576B2 (en) | 2001-03-14 | 2003-01-14 | Board Of Trustees Of The University Of Arkansas | Serum-and steroid-free culture media for cerebellar granule neurons |
JP3728750B2 (ja) * | 2001-11-22 | 2005-12-21 | ニプロ株式会社 | 培養皮膚及びその製造方法 |
WO2003048336A2 (en) | 2001-12-04 | 2003-06-12 | Organogenesis Inc. | Cultured cells from pancreatic islets |
WO2003072128A2 (en) * | 2002-02-22 | 2003-09-04 | Ebi, L.P. | Methods and compositions for treating bone or cartilage defects |
US7741116B2 (en) | 2002-03-06 | 2010-06-22 | University Of Cincinnati | Surgical device for skin therapy or testing |
JP2005521401A (ja) * | 2002-03-27 | 2005-07-21 | イミュネックス・コーポレーション | ポリペプチド産生を増加させる方法 |
AU2003224785B2 (en) * | 2002-03-29 | 2008-12-11 | Singapore Eye Research Institute | Method for growth of human conjunctival tissue equivalents for research, clinical ocular surface transplantation and tissue engineering |
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JP4756816B2 (ja) * | 2003-03-10 | 2011-08-24 | 株式会社アドバンス | 骨再生補綴物の製造方法 |
EP1606395A4 (en) * | 2003-03-17 | 2006-06-14 | Shire Lab Inc | ACCELERATED CULTURE SYSTEM FOR INTESTINAL EPITHELIAL CELL MONOLAYERS |
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KR100567655B1 (ko) * | 2004-04-26 | 2006-04-04 | 사회복지법인 삼성생명공익재단 | 섬유아세포 배양용 배지, 상기 배지를 이용하여 진피대체물을 제조하는 방법 및 그에 의하여 제조되는 진피대체물, 상기 진피 대체물을 이용하여 피부 대체물을제조하는 방법 및 그에 의하여 제조되는 피부 대체물 |
US8252591B2 (en) * | 2004-05-07 | 2012-08-28 | Whitehead Institute For Biomedical Research | Hormone responsive tissue culture system and uses thereof |
US20060121512A1 (en) * | 2004-12-02 | 2006-06-08 | Organ Recovery Systems, Inc. | Biological pathways in progenitor cells |
US20070004036A1 (en) * | 2005-07-01 | 2007-01-04 | Rodolfo Faudoa | Methods and compositions for keratinocyte culture |
US20070003541A1 (en) * | 2005-07-01 | 2007-01-04 | Rodolfo Faudoa | Methods and compositions for therapeutics |
US20070128685A1 (en) * | 2005-07-01 | 2007-06-07 | Rodolfo Faudoa | Methods and compositions for cell culture |
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PT2195023T (pt) | 2007-08-29 | 2018-06-08 | Sanofi Sa | Anticorpos anti-cxcr5 humanizados, seus derivados e suas utilizações |
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BRPI0823049A2 (pt) | 2008-09-07 | 2015-06-16 | Glyconex Inc | Anticorpos para glicoesfingolipídeos tipo 1 anti-estendidos, derivados dos mesmos e uso. |
US20110207175A1 (en) * | 2008-10-06 | 2011-08-25 | Mc2 Cell Aps | Multi-culture bioreactor system |
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US10344260B2 (en) | 2011-09-30 | 2019-07-09 | Amorepacific Corporation | Melanocyte or progenitor cell thereof adapted to keratinocyte, and preparation method thereof |
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US11834680B2 (en) | 2017-06-28 | 2023-12-05 | Rutgers, The State University Of New Jersey | Single kidney cell-derived organoids |
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US4940666A (en) * | 1983-07-15 | 1990-07-10 | University Patents, Inc. | Process and defined medium for growth of human epidermal keratinocyte cells |
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EP0449950A4 (en) * | 1988-12-14 | 1991-10-16 | Us Health | Cell culture medium for human liver epithelial cell line |
US5292655A (en) * | 1990-01-29 | 1994-03-08 | Wille Jr John J | Method for the formation of a histologically-complete skin substitute |
-
1990
- 1990-06-04 CA CA002018228A patent/CA2018228C/en not_active Expired - Lifetime
- 1990-06-04 IL IL9461190A patent/IL94611A/en not_active IP Right Cessation
- 1990-06-05 ES ES90306084T patent/ES2120946T3/es not_active Expired - Lifetime
- 1990-06-05 DK DK90306084T patent/DK0403139T3/da active
- 1990-06-05 DE DE69032549T patent/DE69032549T2/de not_active Expired - Fee Related
- 1990-06-05 JP JP2147191A patent/JP2577114B2/ja not_active Expired - Fee Related
- 1990-06-05 AT AT90306084T patent/ATE169670T1/de not_active IP Right Cessation
- 1990-06-05 EP EP90306084A patent/EP0403139B1/en not_active Expired - Lifetime
-
1995
- 1995-03-28 US US08/412,982 patent/US5712163A/en not_active Expired - Lifetime
Also Published As
Publication number | Publication date |
---|---|
DK0403139T3 (da) | 1999-05-10 |
IL94611A (en) | 1994-12-29 |
DE69032549D1 (de) | 1998-09-17 |
CA2018228A1 (en) | 1990-12-05 |
JPH0387174A (ja) | 1991-04-11 |
ATE169670T1 (de) | 1998-08-15 |
EP0403139A1 (en) | 1990-12-19 |
EP0403139B1 (en) | 1998-08-12 |
DE69032549T2 (de) | 1999-02-04 |
CA2018228C (en) | 1996-02-27 |
US5712163A (en) | 1998-01-27 |
JP2577114B2 (ja) | 1997-01-29 |
IL94611A0 (en) | 1991-04-15 |
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