FR2747395B1 - Procede de primoculture in vitro de cellules epitheliales gastro-intestinales, composition cellulaire obtenue notamment par la mise en oeuvre du procede et applications de ladite composition en tant que systeme de production - Google Patents

Procede de primoculture in vitro de cellules epitheliales gastro-intestinales, composition cellulaire obtenue notamment par la mise en oeuvre du procede et applications de ladite composition en tant que systeme de production

Info

Publication number
FR2747395B1
FR2747395B1 FR9604709A FR9604709A FR2747395B1 FR 2747395 B1 FR2747395 B1 FR 2747395B1 FR 9604709 A FR9604709 A FR 9604709A FR 9604709 A FR9604709 A FR 9604709A FR 2747395 B1 FR2747395 B1 FR 2747395B1
Authority
FR
France
Prior art keywords
primoculture
vitro
implementing
applications
epithelial cells
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
FR9604709A
Other languages
English (en)
Other versions
FR2747395A1 (fr
Inventor
Christian Laboisse
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Universite de Nantes
Original Assignee
Universite de Nantes
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Universite de Nantes filed Critical Universite de Nantes
Priority to FR9604709A priority Critical patent/FR2747395B1/fr
Priority to DE69723264T priority patent/DE69723264T2/de
Priority to EP97918225A priority patent/EP0904353B1/fr
Priority to PCT/FR1997/000668 priority patent/WO1997039109A1/fr
Priority to US09/171,364 priority patent/US6210965B1/en
Priority to AU26428/97A priority patent/AU2642897A/en
Publication of FR2747395A1 publication Critical patent/FR2747395A1/fr
Application granted granted Critical
Publication of FR2747395B1 publication Critical patent/FR2747395B1/fr
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0679—Cells of the gastro-intestinal tract
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/12—Light metals, i.e. alkali, alkaline earth, Be, Al, Mg
    • C12N2500/14—Calcium; Ca chelators; Calcitonin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/90—Serum-free medium, which may still contain naturally-sourced components
    • C12N2500/95—Protein-free medium and culture conditions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2503/00—Use of cells in diagnostics

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
FR9604709A 1996-04-16 1996-04-16 Procede de primoculture in vitro de cellules epitheliales gastro-intestinales, composition cellulaire obtenue notamment par la mise en oeuvre du procede et applications de ladite composition en tant que systeme de production Expired - Fee Related FR2747395B1 (fr)

Priority Applications (6)

Application Number Priority Date Filing Date Title
FR9604709A FR2747395B1 (fr) 1996-04-16 1996-04-16 Procede de primoculture in vitro de cellules epitheliales gastro-intestinales, composition cellulaire obtenue notamment par la mise en oeuvre du procede et applications de ladite composition en tant que systeme de production
DE69723264T DE69723264T2 (de) 1996-04-16 1997-04-15 Verfahren und medium zur zellzüchtung, so erhaltene zellzusammensetzung und ihre anwendungen als produktionssystem und als forschungsmodell
EP97918225A EP0904353B1 (fr) 1996-04-16 1997-04-15 Procede et milieu de culture de cellules, composition cellulaire obtenue et ses applications en tant que systeme de production et de modele d'etude
PCT/FR1997/000668 WO1997039109A1 (fr) 1996-04-16 1997-04-15 Procede et milieu de culture de cellules, composition cellulaire obtenue et ses applications en tant que systeme de production et de modele d'etude
US09/171,364 US6210965B1 (en) 1996-04-16 1997-04-15 Cell culture process and medium, cellular composition obtained and its application as production system and study model
AU26428/97A AU2642897A (en) 1996-04-16 1997-04-15 Cell culture process and medium, cellular composition obtained and its application as production system and study model

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
FR9604709A FR2747395B1 (fr) 1996-04-16 1996-04-16 Procede de primoculture in vitro de cellules epitheliales gastro-intestinales, composition cellulaire obtenue notamment par la mise en oeuvre du procede et applications de ladite composition en tant que systeme de production

Publications (2)

Publication Number Publication Date
FR2747395A1 FR2747395A1 (fr) 1997-10-17
FR2747395B1 true FR2747395B1 (fr) 1998-06-26

Family

ID=9491223

Family Applications (1)

Application Number Title Priority Date Filing Date
FR9604709A Expired - Fee Related FR2747395B1 (fr) 1996-04-16 1996-04-16 Procede de primoculture in vitro de cellules epitheliales gastro-intestinales, composition cellulaire obtenue notamment par la mise en oeuvre du procede et applications de ladite composition en tant que systeme de production

Country Status (1)

Country Link
FR (1) FR2747395B1 (fr)

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4743552A (en) * 1984-09-26 1988-05-10 Sloan-Kettering Institute For Cancer Research Method for growth in tissue culture of normal colonic epithelial cells and method for determination of preneoplastic color cells
FR2634784A1 (fr) * 1988-08-01 1990-02-02 Inst Nat Sante Rech Med Lignees de cellules epitheliales intestinales immortalisees, leurs procedes de preparation et leurs applications en tant que systeme de production de facteurs de croissance et en tant que modele d'etude de la physiopathologie digestive fonctionnelle et cancereuse
CA2018228C (fr) * 1989-06-05 1996-02-27 Nancy L. Parenteau Systemes et milieu de culture cellulaire

Also Published As

Publication number Publication date
FR2747395A1 (fr) 1997-10-17

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ST Notification of lapse

Effective date: 20051230