KR890002387A - 고농도의 히아론산을 얻기 위한 미생물 배양방법 - Google Patents

고농도의 히아론산을 얻기 위한 미생물 배양방법 Download PDF

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Publication number
KR890002387A
KR890002387A KR1019870008243A KR870008243A KR890002387A KR 890002387 A KR890002387 A KR 890002387A KR 1019870008243 A KR1019870008243 A KR 1019870008243A KR 870008243 A KR870008243 A KR 870008243A KR 890002387 A KR890002387 A KR 890002387A
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South Korea
Prior art keywords
culture method
hyaluronic acid
high concentration
culture
cell
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KR1019870008243A
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English (en)
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KR890003677B1 (ko
Inventor
정교민
장이섭
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황영규
태평양화학 주식회사
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Priority to KR1019870008243A priority Critical patent/KR890003677B1/ko
Publication of KR890002387A publication Critical patent/KR890002387A/ko
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Publication of KR890003677B1 publication Critical patent/KR890003677B1/ko

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20Bacteria; Culture media therefor

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Virology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

내용 없음

Description

고농도의 히아론산을 얻기 위한 미생물 배양방법
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
제 1 도는 본 발명에 이용되는 반복 유가 배양의 모식도.

Claims (4)

  1. 스트랩토코커스이퀴 KCTCL873을 배양하여 세포 상태가 후 대수기이고 세포성장 인자가 거의 고갈 되었을 때 배양액을 수거하여 세포만을 선별적으로 재충입하고 배양하는 고농도의 히아론산을 얻기위한 미생물 배양방법.
  2. 제 1 항에서, 세포 재충입후 신선한 배지만을 단계적으로 공급하는 배양방법.
  3. 제 2 항에서, 세포 재충입후 신선한 배지와 포도당을 간헐적 혹은 연속적으로 더불어 첨가하는 배양방법.
  4. 제 3 항에서, 배양액의 포도당 농도를 3-6%로 유지하는 배양방법.
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR1019870008243A 1987-07-29 1987-07-29 고농도의 히아론산을 얻기 위한 미생물 배양방법 KR890003677B1 (ko)

Priority Applications (1)

Application Number Priority Date Filing Date Title
KR1019870008243A KR890003677B1 (ko) 1987-07-29 1987-07-29 고농도의 히아론산을 얻기 위한 미생물 배양방법

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
KR1019870008243A KR890003677B1 (ko) 1987-07-29 1987-07-29 고농도의 히아론산을 얻기 위한 미생물 배양방법

Publications (2)

Publication Number Publication Date
KR890002387A true KR890002387A (ko) 1989-04-10
KR890003677B1 KR890003677B1 (ko) 1989-09-30

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KR1019870008243A KR890003677B1 (ko) 1987-07-29 1987-07-29 고농도의 히아론산을 얻기 위한 미생물 배양방법

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KR (1) KR890003677B1 (ko)

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Publication number Publication date
KR890003677B1 (ko) 1989-09-30

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