KR850003735A - 인터페론의 제조방법 - Google Patents

인터페론의 제조방법 Download PDF

Info

Publication number
KR850003735A
KR850003735A KR1019840007522A KR840007522A KR850003735A KR 850003735 A KR850003735 A KR 850003735A KR 1019840007522 A KR1019840007522 A KR 1019840007522A KR 840007522 A KR840007522 A KR 840007522A KR 850003735 A KR850003735 A KR 850003735A
Authority
KR
South Korea
Prior art keywords
interferon
concentration
medium
ion source
structural gene
Prior art date
Application number
KR1019840007522A
Other languages
English (en)
Other versions
KR920006868B1 (ko
Inventor
가즈아끼 기따노 (외 1)
Original Assignee
구라바야시 이꾸시로
다께다 야꾸힝 고오교 가부시끼 가이샤
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 구라바야시 이꾸시로, 다께다 야꾸힝 고오교 가부시끼 가이샤 filed Critical 구라바야시 이꾸시로
Publication of KR850003735A publication Critical patent/KR850003735A/ko
Application granted granted Critical
Publication of KR920006868B1 publication Critical patent/KR920006868B1/ko

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/555—Interferons [IFN]
    • C07K14/57—IFN-gamma
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/555—Interferons [IFN]
    • C07K14/56—IFN-alpha
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

내용 없음

Description

인터페론의 제조방법
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음

Claims (13)

  1. 인터페론의 구조적 유전자가 삽입된 형질 발현 벡타를 운반하는 미생물을 L-글루탐산 및 철이온원이 함유된 합성 배지에서 배양하고, 배양액으로부터 인터페론을 회수함을 특징으로 하는 인터페론의 제조방법.
  2. 제1항에 있어서, 배지에 아연이온원이 더 함유된 방법.
  3. 제1항에 있어서, 배지에 구리이온원이 더 함유된 방법.
  4. 제2항에 있어서, 배지에 구리이온원이 더 함유된 방법.
  5. 제1항에 있어서, 인터페론의 구조적 유전자가 인체 백혈구 인터페론의 구조적 유전자인 방법.
  6. 제1항에 있어서, 인터페론의 구조적 유전자가 인체 면역 인터페론의 구조적 유전자인 방법.
  7. 제1항에 있어서, 배지에 약 0.1∼10g/ℓ 농도의 L-글루탐산 및 약 10-5∼10-3몰/ℓ 농도의 철이온이 함유된 방법.
  8. 제2항에 있어서, 배지에 약 10-5∼10-3몰/ℓ 농도의 아연 이온원이 함유된 방법.
  9. 제3항에 있어서, 배지에 약 10-5∼10-3몰/ℓ 농도의 구리 이온원이 함유된 방법.
  10. 제4항에 있어서, 배지에 약 10-5∼10-3몰/ℓ 농도의 구리 이온원이 함유된 방법.
  11. 제1항에 있어서, 배지의 탄소원 농도를 약 0.1∼5％로 유지시키면서 배양을 수행하는 방법.
  12. 제1항에 있어서, 배지의 산소 농도를 용해된 산소 포화 농도의 약 5％ 이상에서 유지시키면서 배양을 수행하는 방법.
  13. 제1항에 있어서, 37℃±5℃의 온도에서 배양을 시작하여 세포가 성장함에 따라 온도를 단계적으로 또는 선상으로 하강시켜 최종적으로 23℃±5℃가 되도록 배양을 수행하는 방법.
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR1019840007522A 1983-11-30 1984-11-29 인터페론의 제조방법 KR920006868B1 (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP227750 1983-11-30
JP227750/1983 1983-11-30
JP58227750A JPS60118196A (ja) 1983-11-30 1983-11-30 インタ−フェロンの製造法

Publications (2)

Publication Number Publication Date
KR850003735A true KR850003735A (ko) 1985-06-26
KR920006868B1 KR920006868B1 (ko) 1992-08-21

Family

ID=16865782

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1019840007522A KR920006868B1 (ko) 1983-11-30 1984-11-29 인터페론의 제조방법

Country Status (7)

Country Link
US (1) US4656131A (ko)
EP (1) EP0144064B1 (ko)
JP (1) JPS60118196A (ko)
KR (1) KR920006868B1 (ko)
AT (1) ATE44047T1 (ko)
CA (1) CA1256814A (ko)
DE (1) DE3478699D1 (ko)

Families Citing this family (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4855238A (en) * 1983-12-16 1989-08-08 Genentech, Inc. Recombinant gamma interferons having enhanced stability and methods therefor
FI90990C (fi) * 1984-12-18 1994-04-25 Boehringer Ingelheim Int Rekombinantti-DNA-molekyyli, transformoitu isäntäorganismi ja menetelmä interferonin valmistamiseksi
US5523215A (en) * 1985-03-28 1996-06-04 Chiron Corporation Enhanced purification and expression of insoluble recombinant proteins
EP0200417B1 (en) * 1985-04-30 1992-10-21 Takeda Chemical Industries, Ltd. Production of protein
DE3532134A1 (de) * 1985-09-10 1987-03-12 Basf Ag Verfahren zur herstellung von proteinen bestimmter n-terminaler struktur mit hilfe von prokaryonten
US5210029A (en) * 1985-10-03 1993-05-11 Takeda Chemical Industries, Ltd. Method of producing interleukin-2
DE3642096A1 (de) * 1986-12-10 1988-06-16 Boehringer Ingelheim Int Pferde-(gamma)-interferon
EP0429538B1 (en) * 1988-08-18 2001-11-07 Calgene LLC Plant elongation factor, promoters, coding sequences and uses
US5177011A (en) * 1988-08-18 1993-01-05 Calgene, Inc. Plant elongation factor promoters, coding sequences and uses
US5264365A (en) * 1990-11-09 1993-11-23 Board Of Regents, The University Of Texas System Protease-deficient bacterial strains for production of proteolytically sensitive polypeptides
US5508192A (en) * 1990-11-09 1996-04-16 Board Of Regents, The University Of Texas System Bacterial host strains for producing proteolytically sensitive polypeptides
EP0501692B1 (en) * 1991-02-26 2000-08-02 Zeneca Limited Fermentation processes for the production of peptides by a host
PL176408B1 (pl) * 1992-02-10 1999-05-31 Interferon Sciences Inc Kompozycja naturalnego alfa interferonu z ludzkich leukocytów krwi obwodowej i sposób wytwarzania kompozycji naturalnego alfa interferonu z ludzkich leukocytów krwi obwodowej
US5676942A (en) * 1992-02-10 1997-10-14 Interferon Sciences, Inc. Composition containing human alpha interferon species proteins and method for use thereof
US5487986A (en) * 1994-03-10 1996-01-30 Genzyme Corporation Method for increasing plasmid yield
US5814485A (en) * 1995-06-06 1998-09-29 Chiron Corporation Production of interferon-β (IFN-β) in E. coli
US20030108997A1 (en) * 2001-09-20 2003-06-12 Mahr Klaus Di. Optimizing temperature shifting in high cell density fermentations
JP4149253B2 (ja) * 2002-12-17 2008-09-10 忠行 今中 微生物の低温培養方法
DK2115126T3 (en) * 2007-03-02 2015-05-04 Wyeth Llc Use of copper and glutamate in cell culture for the preparation of polypeptides
ES2558751T3 (es) * 2010-08-30 2016-02-08 F. Hoffmann-La Roche Ag Alimento alcalino

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3065156A (en) * 1961-08-29 1962-11-20 Organon Electrolytic ph regulator
JPS57194787A (en) * 1981-05-28 1982-11-30 Ajinomoto Co Inc Culture medium for animal cell
GB2108510B (en) * 1981-10-03 1984-12-12 Ciba Geigy Ag Dnas, recombinant dnas, hosts containing them, polypeptides and processes for the production thereof
US4450103A (en) * 1982-03-01 1984-05-22 Cetus Corporation Process for recovering human IFN-β from a transformed microorganism
JPS59181224A (ja) * 1983-03-29 1984-10-15 Sumitomo Chem Co Ltd 安定なインタ−フエロン製剤の製法
US4518584A (en) * 1983-04-15 1985-05-21 Cetus Corporation Human recombinant interleukin-2 muteins

Also Published As

Publication number Publication date
CA1256814A (en) 1989-07-04
US4656131A (en) 1987-04-07
JPH0515435B2 (ko) 1993-03-01
EP0144064A2 (en) 1985-06-12
ATE44047T1 (de) 1989-06-15
DE3478699D1 (en) 1989-07-20
EP0144064B1 (en) 1989-06-14
KR920006868B1 (ko) 1992-08-21
JPS60118196A (ja) 1985-06-25
EP0144064A3 (en) 1986-10-08

Similar Documents

Publication Publication Date Title
KR850003735A (ko) 인터페론의 제조방법
MD431C2 (ro) Procedeu de obţinere a interferonului leucocitar
UA8335A1 (uk) СПОСІБ ФЕРМЕНТАЦІЇ МІКРООРГАНІЗМІВ ALKALIGENES ENTROPHUS ДЛЯ ОТРИМАННЯ КЛІТИН, УМІЩУЮЧИХ ПОЛІ-beta-ОКСІМАСЛЯНУ КИСЛОТУ
GB9215834D0 (en) Cell culture
ES471721A1 (es) Un procedimiento para producir interferon
ES8504255A1 (es) Procedimiento para la produccion de una hormona de crecimiento de porcino.
HU9303417D0 (en) Trace element-rich additive, method for preparing same, preparation in which the additive is included and use thereof
EP0210846A3 (en) Method for extracting protein with organic acid
GB1464939A (en) Process for the production of interferon
ES2001862A6 (es) Un metodo para producir el producto de expresion del gen inv de yersinia
ES8507180A1 (es) Un procedimiento para la produccion de glutation
ATE20761T1 (de) Verfahren zur herstellung saurer protease.
ES449890A1 (es) Procedimiento para el tratamiento de proteina de celula sim-ple.
KR880009044A (ko) 묽은 수용액으로부터 소마토트로핀의 회수방법
ATE200913T1 (de) Herstellung von l - ascorbinsäure in mikroorganismen
GB1208225A (en) Process for purifying l-asparaginase
GB1129670A (en) Method of producing alanine by enzyme reaction
ES386831A1 (es) Perfeccionamientos en un procedimiento para producir acido l-glutamico por cultivo.
KR890006821A (ko) 발효에 의한 피루브산의 제조방법
JPS5783283A (en) Novel yeast
GB1244717A (en) Fermentative production of l-lysine
JPS55114291A (en) Prodution of d-alpha-amino acid
JPS5495795A (en) Production of interferon
JPS5682096A (en) Production of l-glutamic acid through fermentation process
JPS5558092A (en) Method of culturing tubercle bacillus

Legal Events

Date Code Title Description
A201 Request for examination
G160 Decision to publish patent application
E701 Decision to grant or registration of patent right
GRNT Written decision to grant
FPAY Annual fee payment

Payment date: 19980811

Year of fee payment: 7

LAPS Lapse due to unpaid annual fee