ES2100882T3 - Microscopio con camara bicolor y metodologia para la tincion y analisis de celulas. - Google Patents

Microscopio con camara bicolor y metodologia para la tincion y analisis de celulas.

Info

Publication number
ES2100882T3
ES2100882T3 ES90905094T ES90905094T ES2100882T3 ES 2100882 T3 ES2100882 T3 ES 2100882T3 ES 90905094 T ES90905094 T ES 90905094T ES 90905094 T ES90905094 T ES 90905094T ES 2100882 T3 ES2100882 T3 ES 2100882T3
Authority
ES
Spain
Prior art keywords
analysis
cells
methodology
staining
microscope
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES90905094T
Other languages
English (en)
Inventor
James William Bacus
Ralph S Hernicz
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Cell Analysis Systems Inc
Original Assignee
Cell Analysis Systems Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cell Analysis Systems Inc filed Critical Cell Analysis Systems Inc
Application granted granted Critical
Publication of ES2100882T3 publication Critical patent/ES2100882T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56966Animal cells
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10Investigating individual particles
    • G01N15/14Optical investigation techniques, e.g. flow cytometry
    • G01N15/1468Optical investigation techniques, e.g. flow cytometry with spatial resolution of the texture or inner structure of the particle
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56966Animal cells
    • G01N33/56972White blood cells
    • GPHYSICS
    • G06COMPUTING; CALCULATING OR COUNTING
    • G06TIMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T7/00Image analysis
    • G06T7/80Analysis of captured images to determine intrinsic or extrinsic camera parameters, i.e. camera calibration
    • G06T7/85Stereo camera calibration
    • GPHYSICS
    • G06COMPUTING; CALCULATING OR COUNTING
    • G06VIMAGE OR VIDEO RECOGNITION OR UNDERSTANDING
    • G06V20/00Scenes; Scene-specific elements
    • G06V20/60Type of objects
    • G06V20/69Microscopic objects, e.g. biological cells or cellular parts
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10Investigating individual particles
    • G01N15/14Optical investigation techniques, e.g. flow cytometry
    • G01N15/1429Signal processing
    • G01N15/1433Signal processing using image recognition
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10Investigating individual particles
    • G01N2015/1006Investigating individual particles for cytology
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10Investigating individual particles
    • G01N15/14Optical investigation techniques, e.g. flow cytometry
    • G01N15/1468Optical investigation techniques, e.g. flow cytometry with spatial resolution of the texture or inner structure of the particle
    • G01N2015/1472Optical investigation techniques, e.g. flow cytometry with spatial resolution of the texture or inner structure of the particle with colour
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N2021/1765Method using an image detector and processing of image signal
    • G01N2021/177Detector of the video camera type
    • G01N2021/1776Colour camera

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Cell Biology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Urology & Nephrology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Pathology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Microbiology (AREA)
  • Theoretical Computer Science (AREA)
  • Dispersion Chemistry (AREA)
  • Multimedia (AREA)
  • Computer Vision & Pattern Recognition (AREA)
  • Investigating Or Analysing Materials By Optical Means (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Solid State Image Pick-Up Elements (AREA)
  • Transforming Light Signals Into Electric Signals (AREA)
  • Analysing Materials By The Use Of Radiation (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)

Abstract

SE PRESENTA UN METODO Y UN APARATO PARA SU USO EN LA REALIZACION DE LA CLASIFICACION AUTOMATIZADA DE CELULAS Y OTROS ESPECIMENES MICROSCOPICOS (52). EL APARATO SUMINISTRA UNA DISPOSICION COMPACTA Y AJUSTABLE QUE FUNCIONA PARA SUMINISTRAR: UN SISTEMA INTERACTIVO DE CLASIFICACION ENTRE EL OPERADOR Y EL APARATO PARA EL ANALISIS DE LAS CELULAS; TECNICAS ALTERNATIVAS PARA CELULAS DIFERENTES, CITOPLASMAS Y POBLACIONES CELULARES; Y UNA IMAGEN O UNA SEPARACION DEL COLOR Y UN ANALISIS MEJORADOS.
ES90905094T 1989-02-24 1990-02-23 Microscopio con camara bicolor y metodologia para la tincion y analisis de celulas. Expired - Lifetime ES2100882T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US07/315,443 US4998284A (en) 1987-11-17 1989-02-24 Dual color camera microscope and methodology for cell staining and analysis

Publications (1)

Publication Number Publication Date
ES2100882T3 true ES2100882T3 (es) 1997-07-01

Family

ID=23224454

Family Applications (1)

Application Number Title Priority Date Filing Date
ES90905094T Expired - Lifetime ES2100882T3 (es) 1989-02-24 1990-02-23 Microscopio con camara bicolor y metodologia para la tincion y analisis de celulas.

Country Status (8)

Country Link
US (1) US4998284A (es)
EP (2) EP0709667A3 (es)
JP (1) JPH05501151A (es)
AT (1) ATE153154T1 (es)
CA (1) CA2045172C (es)
DE (1) DE69030733T2 (es)
ES (1) ES2100882T3 (es)
WO (1) WO1990010276A1 (es)

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Publication number Publication date
EP0534948B1 (en) 1997-05-14
DE69030733T2 (de) 1998-01-22
EP0709667A2 (en) 1996-05-01
EP0534948A4 (en) 1992-09-14
DE69030733D1 (de) 1997-06-19
JPH05501151A (ja) 1993-03-04
CA2045172C (en) 1998-11-24
WO1990010276A1 (en) 1990-09-07
EP0709667A3 (en) 1997-10-22
ATE153154T1 (de) 1997-05-15
EP0534948A1 (en) 1993-04-07
CA2045172A1 (en) 1990-08-25
US4998284A (en) 1991-03-05

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