ES2097768T3 - Amplificacion de la señal de una sonda de adn. - Google Patents

Amplificacion de la señal de una sonda de adn.

Info

Publication number
ES2097768T3
ES2097768T3 ES91105249T ES91105249T ES2097768T3 ES 2097768 T3 ES2097768 T3 ES 2097768T3 ES 91105249 T ES91105249 T ES 91105249T ES 91105249 T ES91105249 T ES 91105249T ES 2097768 T3 ES2097768 T3 ES 2097768T3
Authority
ES
Spain
Prior art keywords
molecule
developer
sequence
branch
nucleic acid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES91105249T
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English (en)
Inventor
David Segev
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Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Application granted granted Critical
Publication of ES2097768T3 publication Critical patent/ES2097768T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/682—Signal amplification

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

LA INVENCION SE REFIERE A UN METODO PARA AMPLIFICAR UN SERIAL DURANTE LA DETECCION DE MOLECULAS DE ACIDO NUCLEICO DE BLANCO QUE CONSISTE EN LOS PASOS SIGUIENTES: A) HIBRIDIZAR UNA PRIMERA SECUENCIA DE UNA SONDA OLIGONUCLEOTIDA PRIMARIA A LAS MOLECULAS DE ACIDO NUCLEICO DE BLANCO EN DONDE LA SONDA PRIMARIA TIENE UN MEDIO PARA ENLAZARSE A UNA MOLECULA DE ACIDO NUCLEICO PUENTE, SIENDO LA MOLECULA PUENTE CAPAZ DE HIBRIDIZAR A POR LO MENOS UNA MOLECULA DE ACIDO NUCLEICO DESARROLLADORA; B) EXPONER LA SONDA PRIMARIA A LA MOLECULA PUENTE Y A UNA PRIMERA MOLECULA DESARROLLADORA Y A UNA SEGUNDA MOLECULA DESARROLLADORA, CONSTANDO CADA MOLECULA DESARROLLADORA DE: I) UNA PRIMERA RAMA QUE CONSTA DE UNA SECUENCIA DE POR LO MENOS DOS NUCLEOTIDOS DIFERENTES Y POR LO MENOS SEIS NUCLEOTIDOS TOTALES COMPLEMENTARIOS A UNA SECUENCIA DE UNA PRIMERA RAMA DE LA OTRA MOLECULA DESARROLLADORA; II) UNA SEGUNDA RAMA QUE CONSTA DE UNA SECUENCIA DE POR LO MENOS DOS NUCLEOTIDOS DIFERENTES Y POR LO MENOS SEIS NUCLEOTIDOS TOTALES COMPLEMENTARIOS A UNA SECUENCIA DE UNA SEGUNDA RAMA DE LA OTRA MOLECULA DESARROLLADORA; Y III) UNA ETIQUETA DETECTABLE O UN MEDIO DE SER CONVERTIDA EN UNA MOLECULA DESARROLLADORA QUE CONTIENE UNA ETIQUETA DETECTABLE; EN DONDE LA PRIMERA MOLECULA DESARROLLADORA Y LA SEGUNDA MOLECULA DESARROLLADORA TIENEN ESTRUCTURAS QUE SON IGUALES O DIFERENTES; Y LLEVAR A CABO LOS PASOS A Y B BAJO CONDICIONES TALES QUE: I) LA MOLECULA PUENTE SE ENLACE A LA SONDA PRIMARIA Y SE HIBRIDICE A LA PRIMERA MOLECULA DESARROLLADORA; Y II) LA PRIMERA MOLECULA DESARROLLADORA ENLAZADA SE HIBRIDICE A UNA SEGUNDA MOLECULA DESARROLLADORA PARA FORMAR UNA CADENA DESARROLLADORA; Y C) DETECTAR LA MOLECULA DESARROLLADORA ETIQUETADA EN LA CADENA DESARROLLADORA.
ES91105249T 1990-04-03 1991-04-03 Amplificacion de la señal de una sonda de adn. Expired - Lifetime ES2097768T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US50362190A 1990-04-03 1990-04-03

Publications (1)

Publication Number Publication Date
ES2097768T3 true ES2097768T3 (es) 1997-04-16

Family

ID=24002849

Family Applications (1)

Application Number Title Priority Date Filing Date
ES91105249T Expired - Lifetime ES2097768T3 (es) 1990-04-03 1991-04-03 Amplificacion de la señal de una sonda de adn.

Country Status (7)

Country Link
US (1) US5437977A (es)
EP (1) EP0450594B1 (es)
JP (2) JP3142888B2 (es)
AT (1) ATE146530T1 (es)
CA (1) CA2039517C (es)
DE (1) DE69123621T2 (es)
ES (1) ES2097768T3 (es)

Families Citing this family (83)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0648281A4 (en) * 1991-09-10 1997-06-04 Jack D Love TARGETED AMPLIFICATION OF DNA AND RNA AND SIGNAL AMPLIFICATION.
US5424413A (en) * 1992-01-22 1995-06-13 Gen-Probe Incorporated Branched nucleic acid probes
US6207368B1 (en) 1992-08-04 2001-03-27 Beckman Coulter, Inc. Methods and reagents for controlling chain extension and ligation chain reactions
US6180338B1 (en) 1992-08-04 2001-01-30 Beckman Coulter, Inc. Method, reagent and kit for the detection and amplification of nucleic acid sequences
FR2710075B1 (fr) * 1993-09-15 1995-10-27 Bio Merieux Réactif et procédé pour la détection d'une séquence nucléotidique avec amplification de signal.
JPH09506629A (ja) * 1993-12-17 1997-06-30 キュビッチョッティ，ロジャー・エス ヌクレオチドに支配された生体分子および多分子薬物の集合並びに装置
CA2140081C (en) 1994-01-13 2008-04-01 Dean L. Engelhardt Process, construct and conjugate for producing multiple nucleic acid copies
US6986985B1 (en) 1994-01-13 2006-01-17 Enzo Life Sciences, Inc. Process for producing multiple nucleic acid copies in vivo using a protein-nucleic acid construct
US5561043A (en) * 1994-01-31 1996-10-01 Trustees Of Boston University Self-assembling multimeric nucleic acid constructs
US7569341B2 (en) * 1994-01-31 2009-08-04 Trustees Of Boston University Nucleic acid directed immobilization arrays and methods of assembly
US6306657B1 (en) * 1994-06-28 2001-10-23 Kalyx Biosciences, Inc. Polynucleotide probe and kit for amplifying signal detection in hybridization assays
CA2126952C (en) * 1994-06-28 2010-12-14 Sithian Pandian Probe, kit, and method of amplification for increasing the sensitivity of nucleic acid hybridization assays
US5871902A (en) * 1994-12-09 1999-02-16 The Gene Pool, Inc. Sequence-specific detection of nucleic acid hybrids using a DNA-binding molecule or assembly capable of discriminating perfect hybrids from non-perfect hybrids
US20030104361A1 (en) 1997-09-29 2003-06-05 Susan Weininger Method of detection of nucleic acids with a specific sequence composition
US5843650A (en) * 1995-05-01 1998-12-01 Segev; David Nucleic acid detection and amplification by chemical linkage of oligonucleotides
DE19537952A1 (de) * 1995-10-12 1997-04-17 Boehringer Mannheim Gmbh Verfahren zum Nachweis eines Analyten
EP0862651A2 (en) * 1995-10-16 1998-09-09 Chiron Corporation Method of screening for factors that modulate gene expression
ATE232559T1 (de) 1996-07-12 2003-02-15 Tm Technologies Inc Verfahren zur signalverstärkung
US5955590A (en) * 1996-07-15 1999-09-21 Worcester Foundation For Biomedical Research Conjugates of minor groove DNA binders with antisense oligonucleotides
US5853993A (en) * 1996-10-21 1998-12-29 Hewlett-Packard Company Signal enhancement method and kit
CA2218440A1 (en) * 1996-12-21 1998-06-21 Boehringer Mannheim Gmbh Method of analysis using signal amplification
AU6659298A (en) 1997-02-18 1998-09-08 Thomas Jefferson University Compositions that bind to pancreatic cancer cells and methods of using the same
AU8601898A (en) * 1997-07-29 1999-02-22 Polyprobe, Inc. Dendritic nucleic acids exhibiting maximal self-assembly
US7135333B1 (en) 1997-08-07 2006-11-14 Thomas Jefferson University Compositions that specifically bind to colorectal cancer cells and methods of using the same
US6120995A (en) * 1997-08-07 2000-09-19 Thomas Jefferson University Compositions that specifically bind to colorectal cancer cells and methods of using the same
JP2002505846A (ja) * 1997-11-06 2002-02-26 モザイク テクノロジーズ シグナル増幅およびプロセッシングのための多重逐次的ポリヌクレオチド置換反応
BR9814272A (pt) * 1997-12-12 2000-10-03 Digene Corp Meio de coleta universal
US20100105572A1 (en) * 1997-12-19 2010-04-29 Kris Richard M High throughput assay system
US6238927B1 (en) 1998-10-05 2001-05-29 Mosaic Technologies, Incorporated Reverse displacement assay for detection of nucleic acid sequences
JP3267576B2 (ja) * 1998-11-09 2002-03-18 三光純薬株式会社 プローブ高分子の形成方法
US6432642B1 (en) 1999-01-15 2002-08-13 Pe Corporation (Ny) Binary probe and clamp composition and methods for a target hybridization detection
AU5103300A (en) * 1999-06-07 2000-12-28 Kenshi Obaru Primers for polymerase reactions
ATE469242T1 (de) * 2000-03-27 2010-06-15 Univ Jefferson Zusammensetzungen und methoden zur identifizierung und zum targeting von krebszellen aus dem verdauungskanal
DE60137104D1 (de) * 2000-03-31 2009-02-05 Sanko Junyaku Kk Sonde zur herstellung einer polymersonde, verfahren zur herstellung einer polymersonde sowie verwendung derselben
US7601497B2 (en) 2000-06-15 2009-10-13 Qiagen Gaithersburg, Inc. Detection of nucleic acids by target-specific hybrid capture method
US7439016B1 (en) 2000-06-15 2008-10-21 Digene Corporation Detection of nucleic acids by type-specific hybrid capture method
AU2001292728A1 (en) * 2000-09-18 2002-03-26 Thomas Jefferson University Compositions and methods for identifying and targeting stomach and esophageal cancer cells
EP1304386B1 (en) * 2000-10-11 2006-10-04 Sanko Junyaku Co., Ltd. Method of constructing self-assembly probes and method of detecting the same
US7727713B2 (en) 2001-06-20 2010-06-01 Nuevolution A/S Templated molecules and methods for using such molecules
US8512953B2 (en) 2001-09-24 2013-08-20 One Lambda Diagnostic probe detection system
JPWO2003029441A1 (ja) * 2001-09-28 2005-01-13 三光純薬株式会社 Ｄｎａチップのシグナル増幅方法
US20040029142A1 (en) * 2002-02-11 2004-02-12 Schon Eric A. Concatenation-based nucleic acid detection compositions and methods
CA2433473A1 (en) * 2002-07-23 2004-01-23 F. Hoffmann-La Roche Ag Fluorescent hybridization probes with reduced background
US10730906B2 (en) 2002-08-01 2020-08-04 Nuevolutions A/S Multi-step synthesis of templated molecules
CN106337046B (zh) * 2002-10-30 2021-03-23 纽韦卢森公司 合成双功能复合物的方法
WO2004056994A2 (en) * 2002-12-19 2004-07-08 Nuevolution A/S Quasirandom structure and function guided synthesis methods
EP1597395A2 (en) 2003-02-21 2005-11-23 Nuevolution A/S Method for producing second-generation library
KR100890885B1 (ko) 2003-03-31 2009-03-31 에프. 호프만-라 로슈 아게 일본 뇌염 바이러스 혈청군의 구성원을 포함하는, 특정플라비바이러스 검출용 조성물 및 방법
US9696298B2 (en) 2003-04-03 2017-07-04 Enzo Life Sciences, Inc. Multisignal reagents for labeling analytes
US7514551B2 (en) 2003-04-03 2009-04-07 Enzo Life Sciences, Inc. Multisignal labeling reagents, and processes and uses therefor
US9156986B2 (en) 2003-04-03 2015-10-13 Enzo Life Sciences, Inc. Multisignal labeling reagents and processes and uses therefor
DK1670939T3 (da) 2003-09-18 2010-03-01 Nuevolution As Fremgangsmåde til opnåelse af strukturel information om et kodet molekyle og fremgangsmåde til udvælgelse af forbindelser
US7306915B2 (en) * 2004-03-22 2007-12-11 Sysmex Corporation Probe set for detection of target substance and detection method using the same
US7745124B2 (en) * 2004-04-28 2010-06-29 Eisai R&D Management Co., Ltd. Hybridization method
US7939251B2 (en) 2004-05-06 2011-05-10 Roche Molecular Systems, Inc. SENP1 as a marker for cancer
DE602005020319D1 (de) * 2004-09-08 2010-05-12 Eisai R&D Man Co Ltd Verfahren zur bildung eines signalsondenpolymers
US7842794B2 (en) 2004-12-17 2010-11-30 Roche Molecular Systems, Inc. Reagents and methods for detecting Neisseria gonorrhoeae
US20070048759A1 (en) * 2005-06-10 2007-03-01 Dan Luo Detection of target molecules with labeled nucleic acid detection molecules
DK2336315T3 (da) * 2005-12-01 2017-11-06 Nuevolution As Fremgangsmåde til enzymatisk kodning ved effektiv syntese af store biblioteker
US8524453B2 (en) * 2006-02-10 2013-09-03 The Brigham And Woman's Hospital, Inc. Lectin complement pathway assays and related compositions and methods
US20070275388A1 (en) * 2006-05-25 2007-11-29 Daniel Ryan Dendrimers that possess a single target annealing site and uses thereof
US9415111B2 (en) 2006-08-11 2016-08-16 Rutgers, The State University Of New Jersey Dual-sensitizer-containing luminescent compounds, conjugates, and uses thereof
EP2180063B1 (en) * 2007-08-14 2013-01-09 Eisai R&D Management Co., Ltd. Method of detecting target substance
US20100256007A1 (en) * 2007-10-24 2010-10-07 Mitsugu Usui Nucleic acid probe, and method of forming probe-polymer
KR20100042082A (ko) * 2008-10-15 2010-04-23 삼성전자주식회사 신호물질의 밀도가 증가되어 있는 고체 지지체, 그를 포함하는 키트 및 그를 이용한 표적물질 검출 방법
US8288520B2 (en) 2008-10-27 2012-10-16 Qiagen Gaithersburg, Inc. Fast results hybrid capture assay and system
JP6108661B2 (ja) * 2009-01-28 2017-04-05 キアジェン ゲイサーズバーグ インコーポレイテッド 配列特異的な大量試料調製方法およびアッセイ法
CA2760542A1 (en) * 2009-05-01 2010-11-04 Qiagen Gaithersburg, Inc. A non-target amplification method for detection of rna splice-forms in a sample
US20100316998A1 (en) * 2009-06-15 2010-12-16 Massachusetts Institute Of Technology Methods and compositions for empirical selection of drugs or other molecules
EP2478087B1 (en) 2009-09-14 2017-01-18 QIAGEN Gaithersburg, Inc. Compositions and methods for recovery of nucleic acids or proteins from tissue samples fixed in cytology media
US9221759B2 (en) 2010-01-13 2015-12-29 Rutgers, The State University Of New Jersey Fluorophore chelated lanthanide luminescent probes with improved quantum efficiency
BR112012018545A2 (pt) 2010-01-29 2016-05-03 Qiagen Gaithersburg Inc método de determinação e confirmação da presença de um hpv em uma amostra
WO2011094514A1 (en) 2010-01-29 2011-08-04 Qiagen Gaithersburg, Inc. Methods and compositions for sequence-specific purification and multiplex analysis of nucleic acids
JP2013528049A (ja) 2010-05-19 2013-07-08 キアゲン ガイサーズバーグ アイエヌシー． 核酸の配列特異的な精製及び多重分析のための方法及び組成物
US9376727B2 (en) 2010-05-25 2016-06-28 Qiagen Gaithersburg, Inc. Fast results hybrid capture assay and associated strategically truncated probes
US8669374B2 (en) * 2010-08-25 2014-03-11 Gene Shen Functionalized cyanine dyes (PEG)
CA2828224A1 (en) 2011-02-24 2012-08-30 Qiagen Gaithersburg, Inc. Materials and methods for detection of hpv nucleic acids
US10385392B2 (en) 2011-12-30 2019-08-20 Abbott Molecular Inc. Nucleic acid hybridization probes
WO2014145620A2 (en) * 2013-03-15 2014-09-18 The Broad Institute, Inc. Novel hybridization probes and uses thereof
EP3063272A2 (en) 2013-10-30 2016-09-07 Green Life Biotech, LLC Pathogenesis quantification systems and treatment methods for citrus greening blight
CN110300806B (zh) * 2016-12-16 2024-03-19 艾瑞特姆有限责任公司 使用连接扩增的分子检测
EP3696275A4 (en) * 2017-10-11 2021-05-26 MGI Tech Co., Ltd. PROCESS FOR IMPROVING THE LOAD AND STABILITY OF NUCLEIC ACIDS ON A SOLID SUPPORT
CN114544965B (zh) * 2020-11-11 2023-04-28 艾克发(北京)生物技术有限公司 一种多重信号放大系统及其在免疫吸附竞争法检测中的应用

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4302204A (en) * 1979-07-02 1981-11-24 The Board Of Trustees Of Leland Stanford Junior University Transfer and detection of nucleic acids
US4762780A (en) * 1984-04-17 1988-08-09 The Regents Of The University Of California Method and composition for screening and diagnosing "HCMV"
US4882669A (en) * 1983-11-28 1989-11-21 Canon Kabushiki Kaisha Multi computer fail safe control apparatus
US4724202A (en) * 1983-12-12 1988-02-09 Molecular Diagnostics, Inc. Use of non-hybridizable nucleic acids for the detection of nucleic acid hybridization
FI71768C (fi) * 1984-02-17 1987-02-09 Orion Yhtymae Oy Foerbaettrade nykleinsyrareagenser och foerfarande foer deras framstaellning.
GB8405437D0 (en) * 1984-03-01 1984-04-04 Amersham Int Plc Detecting polynucleotide sequences
DE3420925A1 (de) * 1984-06-05 1985-12-05 Boehringer Mannheim Gmbh, 6800 Mannheim Verfahren und reagenz zum nachweis einer nukleinsaeure-sequenz
US4683195A (en) * 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
EP0204510B1 (en) * 1985-05-31 1991-07-10 Amoco Corporation Amplification of hybridization signals by employing complementary dna strands
US4868105A (en) * 1985-12-11 1989-09-19 Chiron Corporation Solution phase nucleic acid sandwich assay
EP0245206A1 (en) * 1986-05-05 1987-11-11 IntraCel Corporation Analytical method for detecting and measuring specifically sequenced nucleic acid
IL86164A0 (en) * 1987-04-28 1988-11-15 Tamir Biotechnology Ltd Improved dna probes
CA1339351C (en) * 1987-10-15 1997-08-26 Michael S. Urdea Nucleic acid multimers and amplified nucleic acid hybridization assays using same

Also Published As

Publication number Publication date
JP3142888B2 (ja) 2001-03-07
EP0450594A2 (en) 1991-10-09
JPH0788000A (ja) 1995-04-04
US5437977A (en) 1995-08-01
ATE146530T1 (de) 1997-01-15
EP0450594B1 (en) 1996-12-18
CA2039517A1 (en) 1991-10-04
CA2039517C (en) 2006-11-07
JP2000279186A (ja) 2000-10-10
DE69123621D1 (de) 1997-01-30
EP0450594A3 (en) 1992-01-22
DE69123621T2 (de) 1997-04-17

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