EP3696275A4 - Method for improving loading and stability of nucleic acid on solid support - Google Patents
Method for improving loading and stability of nucleic acid on solid support Download PDFInfo
- Publication number
- EP3696275A4 EP3696275A4 EP17928398.1A EP17928398A EP3696275A4 EP 3696275 A4 EP3696275 A4 EP 3696275A4 EP 17928398 A EP17928398 A EP 17928398A EP 3696275 A4 EP3696275 A4 EP 3696275A4
- Authority
- EP
- European Patent Office
- Prior art keywords
- stability
- nucleic acid
- solid support
- improving loading
- loading
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Plant Pathology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP21213650.1A EP3995590A1 (en) | 2017-10-11 | 2017-10-11 | Method for improving loading and stability of nucleic acid |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
PCT/CN2017/105726 WO2019071471A1 (en) | 2017-10-11 | 2017-10-11 | Method for improving loading and stability of nucleic acid on solid support |
Related Child Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
EP21213650.1A Division EP3995590A1 (en) | 2017-10-11 | 2017-10-11 | Method for improving loading and stability of nucleic acid |
Publications (2)
Publication Number | Publication Date |
---|---|
EP3696275A1 EP3696275A1 (en) | 2020-08-19 |
EP3696275A4 true EP3696275A4 (en) | 2021-05-26 |
Family
ID=66100319
Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
EP17928398.1A Pending EP3696275A4 (en) | 2017-10-11 | 2017-10-11 | Method for improving loading and stability of nucleic acid on solid support |
EP21213650.1A Pending EP3995590A1 (en) | 2017-10-11 | 2017-10-11 | Method for improving loading and stability of nucleic acid |
Family Applications After (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
EP21213650.1A Pending EP3995590A1 (en) | 2017-10-11 | 2017-10-11 | Method for improving loading and stability of nucleic acid |
Country Status (4)
Country | Link |
---|---|
US (1) | US11485966B2 (en) |
EP (2) | EP3696275A4 (en) |
CN (1) | CN111148847B (en) |
WO (1) | WO2019071471A1 (en) |
Families Citing this family (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP3696275A4 (en) * | 2017-10-11 | 2021-05-26 | MGI Tech Co., Ltd. | Method for improving loading and stability of nucleic acid on solid support |
WO2019205135A1 (en) * | 2018-04-28 | 2019-10-31 | 深圳华大基因股份有限公司 | Splint nucleic acid molecule used for performing cyclisation processing on single-chain nucleic acid molecules, and application therefor |
AU2021230282A1 (en) | 2020-03-03 | 2022-09-22 | Pacific Biosciences Of California, Inc. | Methods and compositions for sequencing double stranded nucleic acids |
Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20040248161A1 (en) * | 1999-09-16 | 2004-12-09 | Rothberg Jonathan M. | Method of sequencing a nucleic acid |
WO2013066975A1 (en) * | 2011-11-02 | 2013-05-10 | Complete Genomics, Inc. | Treatment for stabilizing nucleic acid arrays |
WO2016058517A1 (en) * | 2014-10-14 | 2016-04-21 | Bgi Shenzhen Co., Limited | Mate pair library construction |
Family Cites Families (31)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5143854A (en) | 1989-06-07 | 1992-09-01 | Affymax Technologies N.V. | Large scale photolithographic solid phase synthesis of polypeptides and receptor binding screening thereof |
CA2039517C (en) * | 1990-04-03 | 2006-11-07 | David Segev | Dna probe signal amplification |
US5426180A (en) | 1991-03-27 | 1995-06-20 | Research Corporation Technologies, Inc. | Methods of making single-stranded circular oligonucleotides |
US5639603A (en) | 1991-09-18 | 1997-06-17 | Affymax Technologies N.V. | Synthesizing and screening molecular diversity |
US5424413A (en) * | 1992-01-22 | 1995-06-13 | Gen-Probe Incorporated | Branched nucleic acid probes |
SE9400522D0 (en) | 1994-02-16 | 1994-02-16 | Ulf Landegren | Method and reagent for detecting specific nucleotide sequences |
US5641658A (en) | 1994-08-03 | 1997-06-24 | Mosaic Technologies, Inc. | Method for performing amplification of nucleic acid with two primers bound to a single solid support |
US5774305A (en) | 1995-06-07 | 1998-06-30 | Seagate Technology, Inc. | Head gimbal assembly to reduce slider distortion due to thermal stress |
US5854033A (en) | 1995-11-21 | 1998-12-29 | Yale University | Rolling circle replication reporter systems |
AU714486B2 (en) | 1995-11-21 | 2000-01-06 | Yale University | Unimolecular segment amplification and detection |
IL141148A0 (en) | 1998-07-30 | 2002-02-10 | Solexa Ltd | Arrayed biomolecules and their use in sequencing |
EP1218545B1 (en) | 1999-08-18 | 2012-01-25 | Illumina, Inc. | Methods for preparing oligonucleotide solutions |
US6383783B1 (en) * | 1999-09-21 | 2002-05-07 | 3M Innovative Properties Company | Nucleic acid isolation by adhering to hydrophobic solid phase and removing with nonionic surfactant |
AU2001241723A1 (en) | 2000-02-25 | 2001-09-03 | Affymetrix, Inc. | Methods for multi-stage solid phase amplification of nucleic acids |
WO2006073504A2 (en) | 2004-08-04 | 2006-07-13 | President And Fellows Of Harvard College | Wobble sequencing |
US20070048759A1 (en) * | 2005-06-10 | 2007-03-01 | Dan Luo | Detection of target molecules with labeled nucleic acid detection molecules |
US7709197B2 (en) | 2005-06-15 | 2010-05-04 | Callida Genomics, Inc. | Nucleic acid analysis by random mixtures of non-overlapping fragments |
WO2007120208A2 (en) | 2005-11-14 | 2007-10-25 | President And Fellows Of Harvard College | Nanogrid rolling circle dna sequencing |
JP5180845B2 (en) | 2006-02-24 | 2013-04-10 | カリダ・ジェノミックス・インコーポレイテッド | High-throughput genomic sequencing on DNA arrays |
WO2007111937A1 (en) | 2006-03-23 | 2007-10-04 | Applera Corporation | Directed enrichment of genomic dna for high-throughput sequencing |
WO2008076414A2 (en) * | 2006-12-15 | 2008-06-26 | New York University | Self-replicating materials |
US9347092B2 (en) | 2009-02-25 | 2016-05-24 | Roche Molecular System, Inc. | Solid support for high-throughput nucleic acid analysis |
US9796749B2 (en) * | 2011-08-05 | 2017-10-24 | President And Fellows Of Harvard College | Compositions and methods relating to nucleic acid nano- and micro-technology |
EP4026912A1 (en) | 2012-05-10 | 2022-07-13 | The General Hospital Corporation | Methods for determining a nucleotide sequence |
WO2014071388A1 (en) * | 2012-11-05 | 2014-05-08 | Gartner Zev Jordan | Methods of patterning cells on a surface of a substrate and programmed assembly of three-dimensional living tissues |
GB2546833B (en) * | 2013-08-28 | 2018-04-18 | Cellular Res Inc | Microwell for single cell analysis comprising single cell and single bead oligonucleotide capture labels |
US11124846B2 (en) * | 2014-07-14 | 2021-09-21 | President And Fellows Of Harvard College | Compositions comprising riboregulators and methods of use thereof |
FI3901281T3 (en) * | 2015-04-10 | 2023-01-31 | Spatially distinguished, multiplex nucleic acid analysis of biological specimens | |
US10948484B2 (en) * | 2016-04-11 | 2021-03-16 | Veravas, Inc. | Sample depletion and enrichment to improve the quality of diagnostic test results |
SG11201909697TA (en) * | 2017-05-01 | 2019-11-28 | Illumina Inc | Optimal index sequences for multiplex massively parallel sequencing |
EP3696275A4 (en) * | 2017-10-11 | 2021-05-26 | MGI Tech Co., Ltd. | Method for improving loading and stability of nucleic acid on solid support |
-
2017
- 2017-10-11 EP EP17928398.1A patent/EP3696275A4/en active Pending
- 2017-10-11 EP EP21213650.1A patent/EP3995590A1/en active Pending
- 2017-10-11 US US16/754,713 patent/US11485966B2/en active Active
- 2017-10-11 WO PCT/CN2017/105726 patent/WO2019071471A1/en unknown
- 2017-10-11 CN CN201780094656.6A patent/CN111148847B/en active Active
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20040248161A1 (en) * | 1999-09-16 | 2004-12-09 | Rothberg Jonathan M. | Method of sequencing a nucleic acid |
WO2013066975A1 (en) * | 2011-11-02 | 2013-05-10 | Complete Genomics, Inc. | Treatment for stabilizing nucleic acid arrays |
WO2016058517A1 (en) * | 2014-10-14 | 2016-04-21 | Bgi Shenzhen Co., Limited | Mate pair library construction |
Non-Patent Citations (2)
Title |
---|
MAK SARAH SIU TZE ET AL: "Comparative performance of the BGISEQ-500 vs Illumina HiSeq2500 sequencing platforms for palaeogenomic sequencing", GIGASCIENCE, vol. 6, no. 8, 26 June 2017 (2017-06-26), XP055795958, Retrieved from the Internet <URL:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5570000/pdf/gix049.pdf> DOI: 10.1093/gigascience/gix049 * |
See also references of WO2019071471A1 * |
Also Published As
Publication number | Publication date |
---|---|
US11485966B2 (en) | 2022-11-01 |
US20200255821A1 (en) | 2020-08-13 |
CN111148847A (en) | 2020-05-12 |
CN111148847B (en) | 2024-05-07 |
EP3995590A1 (en) | 2022-05-11 |
EP3696275A1 (en) | 2020-08-19 |
WO2019071471A1 (en) | 2019-04-18 |
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Legal Events
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STAA | Information on the status of an ep patent application or granted ep patent |
Free format text: STATUS: THE INTERNATIONAL PUBLICATION HAS BEEN MADE |
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PUAI | Public reference made under article 153(3) epc to a published international application that has entered the european phase |
Free format text: ORIGINAL CODE: 0009012 |
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STAA | Information on the status of an ep patent application or granted ep patent |
Free format text: STATUS: REQUEST FOR EXAMINATION WAS MADE |
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17P | Request for examination filed |
Effective date: 20200323 |
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AK | Designated contracting states |
Kind code of ref document: A1 Designated state(s): AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR |
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AX | Request for extension of the european patent |
Extension state: BA ME |
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DAV | Request for validation of the european patent (deleted) | ||
DAX | Request for extension of the european patent (deleted) | ||
A4 | Supplementary search report drawn up and despatched |
Effective date: 20210426 |
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RIC1 | Information provided on ipc code assigned before grant |
Ipc: C12Q 1/68 20180101AFI20210421BHEP Ipc: C12N 15/10 20060101ALI20210421BHEP |