EP3607064A4 - Method and kit for targeted enrichment of nucleic acids - Google Patents

Method and kit for targeted enrichment of nucleic acids Download PDF

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Publication number
EP3607064A4
EP3607064A4 EP18781045.2A EP18781045A EP3607064A4 EP 3607064 A4 EP3607064 A4 EP 3607064A4 EP 18781045 A EP18781045 A EP 18781045A EP 3607064 A4 EP3607064 A4 EP 3607064A4
Authority
EP
European Patent Office
Prior art keywords
kit
nucleic acids
targeted enrichment
enrichment
targeted
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
EP18781045.2A
Other languages
German (de)
French (fr)
Other versions
EP3607064A1 (en
Inventor
Qing Wang
Ming Zhang
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Complete Omics International Inc
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Priority claimed from PCT/US2018/019788 external-priority patent/WO2018186947A1/en
Publication of EP3607064A1 publication Critical patent/EP3607064A1/en
Publication of EP3607064A4 publication Critical patent/EP3607064A4/en
Pending legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1065—Preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis, tagged polynucleotides, gene tags
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR

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  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
EP18781045.2A 2017-04-06 2018-02-26 Method and kit for targeted enrichment of nucleic acids Pending EP3607064A4 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201762482189P 2017-04-06 2017-04-06
PCT/US2018/016778 WO2018186930A1 (en) 2017-04-06 2018-02-04 Method and kit for constructing nucleic acid library
PCT/US2018/019788 WO2018186947A1 (en) 2017-04-06 2018-02-26 Method and kit for targeted enrichment of nucleic acids

Publications (2)

Publication Number Publication Date
EP3607064A1 EP3607064A1 (en) 2020-02-12
EP3607064A4 true EP3607064A4 (en) 2020-06-03

Family

ID=63713550

Family Applications (2)

Application Number Title Priority Date Filing Date
EP18781272.2A Active EP3607065B1 (en) 2017-04-06 2018-02-04 Method and kit for constructing nucleic acid library
EP18781045.2A Pending EP3607064A4 (en) 2017-04-06 2018-02-26 Method and kit for targeted enrichment of nucleic acids

Family Applications Before (1)

Application Number Title Priority Date Filing Date
EP18781272.2A Active EP3607065B1 (en) 2017-04-06 2018-02-04 Method and kit for constructing nucleic acid library

Country Status (5)

Country Link
EP (2) EP3607065B1 (en)
CN (2) CN110719958B (en)
DK (1) DK3607065T3 (en)
FI (1) FI3607065T3 (en)
WO (1) WO2018186930A1 (en)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109957562B (en) * 2019-03-06 2019-12-06 南京诺唯赞生物科技有限公司 Method and kit for quickly constructing transcriptome sequencing library
AU2020343336A1 (en) * 2019-09-03 2022-03-24 Sara L. SAWYER Systems, methods, and compositions for the rapid early-detection of host RNA biomarkers of infection and early identification of COVID-19 coronavirus infection in humans
CN111455469B (en) * 2020-04-07 2023-08-18 深圳易倍科华生物科技有限公司 Single-chain rapid library construction method and library construction instrument
CN111979583B (en) * 2020-09-10 2023-09-12 杭州求臻医学检验实验室有限公司 Construction method and application of single-stranded nucleic acid molecule high-throughput sequencing library
CN112649599B (en) * 2020-12-18 2022-08-16 郑州安图生物工程股份有限公司 Method for indirectly marking and sealing colloidal gold
CN112941147B (en) * 2021-03-02 2024-06-04 深圳市睿法生物科技有限公司 High-fidelity target gene library construction method and kit thereof
CN113921089B (en) * 2021-11-22 2022-04-08 北京安智因生物技术有限公司 Method and system for confirming updating frequency of IVD gene annotation database
CN115083516B (en) * 2022-07-13 2023-03-21 北京先声医学检验实验室有限公司 Panel design and evaluation method for detecting gene fusion based on targeted RNA sequencing technology
CN117327785B (en) * 2023-12-01 2024-03-12 天津金域医学检验实验室有限公司 Primer set, method and application for SBDS gene mutation detection

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001006012A1 (en) * 1999-07-14 2001-01-25 Packard Bioscience Company Derivative nucleic acids and uses thereof
WO2010117817A2 (en) * 2009-03-30 2010-10-14 Life Technologies Corporation Methods for generating target specific probes for solution based capture
US20110171740A1 (en) * 2010-01-08 2011-07-14 Yong Chen Bioassays based on polymeric sequence probe

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5219726A (en) * 1989-06-02 1993-06-15 The Salk Institute For Biological Studies Physical mapping of complex genomes
JP2775346B2 (en) * 1992-04-03 1998-07-16 アプライド バイオシステムズ，インコーポレイテッド Probe composition and method
US6458530B1 (en) * 1996-04-04 2002-10-01 Affymetrix Inc. Selecting tag nucleic acids
EP1590477B1 (en) * 2003-01-29 2009-07-29 454 Corporation Methods of amplifying and sequencing nucleic acids
US7833716B2 (en) * 2006-06-06 2010-11-16 Gen-Probe Incorporated Tagged oligonucleotides and their use in nucleic acid amplification methods
EP2221387A1 (en) * 2009-02-19 2010-08-25 Université de la Méditerranée Fibrosis susceptibility gene and uses thereof
US20160040218A1 (en) * 2013-03-14 2016-02-11 The Broad Institute, Inc. Selective Purification of RNA and RNA-Bound Molecular Complexes
EP2976435B1 (en) * 2013-03-19 2017-10-25 Directed Genomics, LLC Enrichment of target sequences
EP3209675A4 (en) * 2014-10-22 2018-04-18 Roka Bioscience, Inc. Compositions and methods for the detection of nucleic acids
KR101858344B1 (en) * 2015-06-01 2018-05-16 연세대학교 산학협력단 Method of next generation sequencing using adapter comprising barcode sequence

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001006012A1 (en) * 1999-07-14 2001-01-25 Packard Bioscience Company Derivative nucleic acids and uses thereof
WO2010117817A2 (en) * 2009-03-30 2010-10-14 Life Technologies Corporation Methods for generating target specific probes for solution based capture
US20110171740A1 (en) * 2010-01-08 2011-07-14 Yong Chen Bioassays based on polymeric sequence probe

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
LAMB ET AL: "A PRACTICAL APPROACH", GENE PROBES, XX, XX, 1 April 1995 (1995-04-01), pages 1 - 18, XP002929707 *
QING WANG ET AL: "Targeted sequencing of both DNA strands barcoded and captured individually by RNA probes to identify genome-wide ultra-rare mutations", SCIENTIFIC REPORTS, vol. 7, no. 1, 13 June 2017 (2017-06-13), XP055551336, DOI: 10.1038/s41598-017-03448-8 *

Also Published As

Publication number Publication date
CN110719957B (en) 2023-07-28
EP3607065B1 (en) 2023-03-15
CN110719957A (en) 2020-01-21
EP3607065A4 (en) 2020-04-15
EP3607064A1 (en) 2020-02-12
CN110719958B (en) 2023-07-18
DK3607065T3 (en) 2023-06-19
WO2018186930A1 (en) 2018-10-11
FI3607065T3 (en) 2023-06-16
EP3607065A1 (en) 2020-02-12
CN110719958A (en) 2020-01-21

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