EP3607064A4 - Verfahren und kit zur gezielten anreicherung von nukleinsäuren - Google Patents
Verfahren und kit zur gezielten anreicherung von nukleinsäuren Download PDFInfo
- Publication number
- EP3607064A4 EP3607064A4 EP18781045.2A EP18781045A EP3607064A4 EP 3607064 A4 EP3607064 A4 EP 3607064A4 EP 18781045 A EP18781045 A EP 18781045A EP 3607064 A4 EP3607064 A4 EP 3607064A4
- Authority
- EP
- European Patent Office
- Prior art keywords
- kit
- nucleic acids
- targeted enrichment
- enrichment
- targeted
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1065—Preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis, tagged polynucleotides, gene tags
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Crystallography & Structural Chemistry (AREA)
- Plant Pathology (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Bioinformatics & Computational Biology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US201762482189P | 2017-04-06 | 2017-04-06 | |
PCT/US2018/016778 WO2018186930A1 (en) | 2017-04-06 | 2018-02-04 | Method and kit for constructing nucleic acid library |
PCT/US2018/019788 WO2018186947A1 (en) | 2017-04-06 | 2018-02-26 | Method and kit for targeted enrichment of nucleic acids |
Publications (2)
Publication Number | Publication Date |
---|---|
EP3607064A1 EP3607064A1 (de) | 2020-02-12 |
EP3607064A4 true EP3607064A4 (de) | 2020-06-03 |
Family
ID=63713550
Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
EP18781272.2A Active EP3607065B1 (de) | 2017-04-06 | 2018-02-04 | Verfahren und kit zum aufbau einer nukleinsäurebibliothek |
EP18781045.2A Pending EP3607064A4 (de) | 2017-04-06 | 2018-02-26 | Verfahren und kit zur gezielten anreicherung von nukleinsäuren |
Family Applications Before (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
EP18781272.2A Active EP3607065B1 (de) | 2017-04-06 | 2018-02-04 | Verfahren und kit zum aufbau einer nukleinsäurebibliothek |
Country Status (5)
Country | Link |
---|---|
EP (2) | EP3607065B1 (de) |
CN (2) | CN110719958B (de) |
DK (1) | DK3607065T3 (de) |
FI (1) | FI3607065T3 (de) |
WO (1) | WO2018186930A1 (de) |
Families Citing this family (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN109957562B (zh) * | 2019-03-06 | 2019-12-06 | 南京诺唯赞生物科技有限公司 | 一种快速构建转录组测序文库的方法及试剂盒 |
CN116171198A (zh) * | 2019-09-03 | 2023-05-26 | 科罗拉多大学董事会,法人团体 | 用于快速早期检测感染的宿主rna生物标志物和早期鉴定人的covid-19冠状病毒感染的系统、方法和组合物 |
CN111455469B (zh) * | 2020-04-07 | 2023-08-18 | 深圳易倍科华生物科技有限公司 | 一种单链快速建库方法及建库仪器 |
CN111979583B (zh) * | 2020-09-10 | 2023-09-12 | 杭州求臻医学检验实验室有限公司 | 一种单链核酸分子高通量测序文库的构建方法及其应用 |
CN112649599B (zh) * | 2020-12-18 | 2022-08-16 | 郑州安图生物工程股份有限公司 | 一种胶体金间接标记、封闭的方法 |
CN112941147B (zh) * | 2021-03-02 | 2024-06-04 | 深圳市睿法生物科技有限公司 | 一种高保真靶标基因建库方法及其试剂盒 |
CN113921089B (zh) * | 2021-11-22 | 2022-04-08 | 北京安智因生物技术有限公司 | 一种用于确认ivd基因注释数据库更新频率的方法及系统 |
CN115083516B (zh) * | 2022-07-13 | 2023-03-21 | 北京先声医学检验实验室有限公司 | 一种基于靶向RNA测序技术检测基因融合的Panel设计和评估方法 |
CN117327785B (zh) * | 2023-12-01 | 2024-03-12 | 天津金域医学检验实验室有限公司 | 一种用于sbds基因突变检测的引物组、方法及应用 |
Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2001006012A1 (en) * | 1999-07-14 | 2001-01-25 | Packard Bioscience Company | Derivative nucleic acids and uses thereof |
WO2010117817A2 (en) * | 2009-03-30 | 2010-10-14 | Life Technologies Corporation | Methods for generating target specific probes for solution based capture |
US20110171740A1 (en) * | 2010-01-08 | 2011-07-14 | Yong Chen | Bioassays based on polymeric sequence probe |
Family Cites Families (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5219726A (en) * | 1989-06-02 | 1993-06-15 | The Salk Institute For Biological Studies | Physical mapping of complex genomes |
EP0636186B1 (de) * | 1992-04-03 | 1998-11-25 | The Perkin-Elmer Corporation | Proben zusammensetzung und verfahren |
US6458530B1 (en) * | 1996-04-04 | 2002-10-01 | Affymetrix Inc. | Selecting tag nucleic acids |
CA2513541A1 (en) * | 2003-01-29 | 2004-08-19 | 454 Corporation | Method for preparing single-stranded dna libraries |
US7833716B2 (en) * | 2006-06-06 | 2010-11-16 | Gen-Probe Incorporated | Tagged oligonucleotides and their use in nucleic acid amplification methods |
EP2221387A1 (de) * | 2009-02-19 | 2010-08-25 | Université de la Méditerranée | Fibroseanfälligkeitsgen und Verwendungen davon |
US20160040218A1 (en) * | 2013-03-14 | 2016-02-11 | The Broad Institute, Inc. | Selective Purification of RNA and RNA-Bound Molecular Complexes |
US9708658B2 (en) * | 2013-03-19 | 2017-07-18 | New England Biolabs, Inc. | Enrichment of target sequences |
WO2016065192A1 (en) * | 2014-10-22 | 2016-04-28 | Roka Bioscience, Inc. | Compositions and methods for the detection of nucleic acids |
WO2016195382A1 (ko) * | 2015-06-01 | 2016-12-08 | 연세대학교 산학협력단 | 바코드 서열을 포함하는 어댑터를 이용한 차세대 염기서열 분석 방법 |
-
2018
- 2018-02-04 DK DK18781272.2T patent/DK3607065T3/da active
- 2018-02-04 WO PCT/US2018/016778 patent/WO2018186930A1/en unknown
- 2018-02-04 FI FIEP18781272.2T patent/FI3607065T3/fi active
- 2018-02-04 CN CN201880037683.4A patent/CN110719958B/zh active Active
- 2018-02-04 EP EP18781272.2A patent/EP3607065B1/de active Active
- 2018-02-26 CN CN201880037641.0A patent/CN110719957B/zh active Active
- 2018-02-26 EP EP18781045.2A patent/EP3607064A4/de active Pending
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2001006012A1 (en) * | 1999-07-14 | 2001-01-25 | Packard Bioscience Company | Derivative nucleic acids and uses thereof |
WO2010117817A2 (en) * | 2009-03-30 | 2010-10-14 | Life Technologies Corporation | Methods for generating target specific probes for solution based capture |
US20110171740A1 (en) * | 2010-01-08 | 2011-07-14 | Yong Chen | Bioassays based on polymeric sequence probe |
Non-Patent Citations (2)
Title |
---|
LAMB ET AL: "A PRACTICAL APPROACH", GENE PROBES, XX, XX, 1 April 1995 (1995-04-01), pages 1 - 18, XP002929707 * |
QING WANG ET AL: "Targeted sequencing of both DNA strands barcoded and captured individually by RNA probes to identify genome-wide ultra-rare mutations", SCIENTIFIC REPORTS, vol. 7, no. 1, 13 June 2017 (2017-06-13), XP055551336, DOI: 10.1038/s41598-017-03448-8 * |
Also Published As
Publication number | Publication date |
---|---|
FI3607065T3 (fi) | 2023-06-16 |
EP3607065A4 (de) | 2020-04-15 |
EP3607065A1 (de) | 2020-02-12 |
CN110719957A (zh) | 2020-01-21 |
EP3607065B1 (de) | 2023-03-15 |
CN110719957B (zh) | 2023-07-28 |
CN110719958B (zh) | 2023-07-18 |
CN110719958A (zh) | 2020-01-21 |
WO2018186930A1 (en) | 2018-10-11 |
DK3607065T3 (da) | 2023-06-19 |
EP3607064A1 (de) | 2020-02-12 |
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Extension state: BA ME |
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A4 | Supplementary search report drawn up and despatched |
Effective date: 20200507 |
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RIC1 | Information provided on ipc code assigned before grant |
Ipc: C12N 15/10 20060101AFI20200429BHEP Ipc: C12Q 1/68 20180101ALI20200429BHEP |
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17Q | First examination report despatched |
Effective date: 20220630 |
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RAP1 | Party data changed (applicant data changed or rights of an application transferred) |
Owner name: COMPLETE OMICS INC. |
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RAP1 | Party data changed (applicant data changed or rights of an application transferred) |
Owner name: COMPLETE OMICS INTERNATIONAL INC. |