ES2084052T3 - Secrecion de derivados de hirudina. - Google Patents
Secrecion de derivados de hirudina.Info
- Publication number
- ES2084052T3 ES2084052T3 ES91104422T ES91104422T ES2084052T3 ES 2084052 T3 ES2084052 T3 ES 2084052T3 ES 91104422 T ES91104422 T ES 91104422T ES 91104422 T ES91104422 T ES 91104422T ES 2084052 T3 ES2084052 T3 ES 2084052T3
- Authority
- ES
- Spain
- Prior art keywords
- hirudin
- recombinant vector
- hirudin derivative
- derivatives
- tyr
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1048—Glycosyltransferases (2.4)
- C12N9/1051—Hexosyltransferases (2.4.1)
- C12N9/1074—Cyclomaltodextrin glucanotransferase (2.4.1.19)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P7/00—Drugs for disorders of the blood or the extracellular fluid
- A61P7/02—Antithrombotic agents; Anticoagulants; Platelet aggregation inhibitors
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/81—Protease inhibitors
- C07K14/815—Protease inhibitors from leeches, e.g. hirudin, eglin
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- Medicinal Chemistry (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Gastroenterology & Hepatology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Animal Behavior & Ethology (AREA)
- Pharmacology & Pharmacy (AREA)
- Diabetes (AREA)
- Hematology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Materials For Medical Uses (AREA)
- Solid-Sorbent Or Filter-Aiding Compositions (AREA)
- Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
Abstract
UN METODO PARA LA OBTENCION DE DERIVADOS DE HIRUDINA SE OBTIENE MEDIANTE MUTACIONES DE E. DE SECRECION COLI, EN DONDE 1) SE CONSTRUYE UN VECTOR RECOMBINADO, EN DONDE SE ENCUENTRA EL GEN CODIFICADO PARA EL DERIVADO DE HURIDINA, DIRECTAMENTE DETRAS DE LA SECCION DE ADN, QUE CODIFICA LA SEÑAL PEPTIDA BACTEREOLOGICA, 2) SE TRANSFORMA CON UN VECTOR RECOMBINADO Y CONSTRUIDO EN PASO 1 UN MUTANTE DE SECRETO DE COLI E., 3) SE CULTIVAN LAS CELULAS TRANSFORMADAS EN UN MEDIO Y 4) EL DERIVADO DE HIRUDINA OBTENIDO DEL MEDIO, ASI COMO EL VECTOR RECOMBINADO, EL CUAL CONTIENE UNA O VARIAS COPIAS DE UN CONSTRUCTOR DE GEN, QUE PARA UNA PROTEINA, CONSTA DE UNA SEÑAL DE PEPTIDO BACTERIOLOGICA Y DE UN DERIVADO DE HIRUDINA, CODIFICADO Y UN DERIVADO DE HIRUDINA CON UNA FRECUENCIA DE ACIDO DE AMINO DE UN TERMINAL N Y QUE REPRESENTA A-THR-TYRTHR-ASP, EN DONDE A REPRESENTA ALA, GIN, HIS, PHE, TYR, GLU, SER, ASP, O ASN.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE4009268A DE4009268A1 (de) | 1990-03-22 | 1990-03-22 | Sekretion von hirudinderivaten |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ES2084052T3 true ES2084052T3 (es) | 1996-05-01 |
Family
ID=6402843
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES91104422T Expired - Lifetime ES2084052T3 (es) | 1990-03-22 | 1991-03-21 | Secrecion de derivados de hirudina. |
Country Status (23)
| Country | Link |
|---|---|
| US (1) | US5919895A (es) |
| EP (1) | EP0448093B1 (es) |
| JP (1) | JPH07106157B2 (es) |
| KR (1) | KR950007510B1 (es) |
| CN (1) | CN1067104C (es) |
| AT (1) | ATE135042T1 (es) |
| AU (1) | AU640212B2 (es) |
| BR (1) | BR9101104A (es) |
| CA (1) | CA2038888C (es) |
| DE (2) | DE4009268A1 (es) |
| DK (1) | DK0448093T3 (es) |
| ES (1) | ES2084052T3 (es) |
| FI (1) | FI100250B (es) |
| GR (1) | GR3019946T3 (es) |
| HU (1) | HU214368B (es) |
| IE (1) | IE71174B1 (es) |
| IL (1) | IL97323A (es) |
| NO (1) | NO308543B1 (es) |
| NZ (2) | NZ237437A (es) |
| PH (1) | PH30485A (es) |
| PT (1) | PT97093B (es) |
| RU (1) | RU2118365C1 (es) |
| ZA (1) | ZA912013B (es) |
Families Citing this family (23)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE3835815A1 (de) * | 1988-10-21 | 1990-04-26 | Hoechst Ag | Neue isohirudine |
| DE4140381A1 (de) * | 1991-12-07 | 1993-06-09 | Hoechst Ag, 6230 Frankfurt, De | Neue synthetische isohirudine mit verbesserter stabilitaet |
| SE9203753D0 (sv) * | 1992-12-11 | 1992-12-11 | Kabi Pharmacia Ab | Expression system for producing apolipoprotein ai-m |
| AU3673495A (en) | 1995-04-12 | 1996-10-30 | Kyowa Hakko Kogyo Co. Ltd. | Plant cell growth factor |
| JP3746847B2 (ja) * | 1996-08-02 | 2006-02-15 | 協和醗酵工業株式会社 | 植物細胞増殖因子 |
| DE19915938A1 (de) | 1999-04-09 | 2000-10-19 | Aventis Pharma Gmbh | Herstellung von pankreatischer Procarboxypeptidase B, Isoformen und Muteinen davon und ihre Verwendung |
| DE19944870A1 (de) * | 1999-09-18 | 2001-03-29 | Aventis Pharma Gmbh | Signalsequenzen zur Herstellung von Leu-Hirudin über Sekretion durch E. coli in das Kulturmedium |
| DE10033195A1 (de) * | 2000-07-07 | 2002-03-21 | Aventis Pharma Gmbh | Bifunktionale Fusionsproteine aus Hirudin und TAP |
| US7202059B2 (en) | 2001-02-20 | 2007-04-10 | Sanofi-Aventis Deutschland Gmbh | Fusion proteins capable of being secreted into a fermentation medium |
| US7638618B2 (en) | 2001-02-20 | 2009-12-29 | Sanofi-Aventis Deutschland Gmbh | Nucleic acids encoding a hirudin and pro-insulin as superscretable peptides and for parallel improvement of the exported forms of one or more polypeptides of interest |
| DE10108211A1 (de) * | 2001-02-20 | 2002-08-22 | Aventis Pharma Gmbh | Verwendung von Fusionsproteinen, deren N-terminaler Anteil aus einem Hirudinderivat besteht, zur Herstellung rekombinanter Proteine über Sekretion durch Hefen |
| DE102006004871A1 (de) | 2006-02-02 | 2007-08-09 | Wacker Chemie Ag | Mikroorganismenstamm zur Produktion von rekombinanten Proteinen |
| DE502006006800D1 (de) * | 2006-09-22 | 2010-06-02 | Wacker Chemie Ag | Verfahren zur fermentativen Herstellung von Proteinen |
| DE102006044841A1 (de) | 2006-09-22 | 2008-04-03 | Wacker Chemie Ag | Signalpeptid zur Produktion von rekombinanten Proteinen |
| DK1903115T3 (da) | 2006-09-22 | 2011-05-23 | Wacker Chemie Ag | Fremgangsmåde til fermentativ fremstilling af antistoffer |
| EP1905839B2 (de) * | 2006-09-22 | 2019-07-10 | Wacker Chemie AG | Verfahren zur fermentativen Herstellung von Proteinen |
| DE102006050332A1 (de) * | 2006-10-25 | 2008-04-30 | Wacker Chemie Ag | DNS-Konstrukt und Verfahren zur fermentativen Herstellung von Fusionsproteinen |
| DE102008063900A1 (de) | 2008-12-19 | 2010-06-24 | Wacker Chemie Ag | Verfahren zur fermentativen Herstellung von heterologen Proteinen mittels Escherichia coli |
| CN103848914B (zh) * | 2012-11-29 | 2018-08-28 | 河北以岭医药研究院有限公司 | 一种具抗凝活性的菲牛蛭素多肽及其制备方法与用途 |
| CN103059130A (zh) * | 2012-12-22 | 2013-04-24 | 浙江工业大学 | 一种水蛭素突变体及重组基因工程菌 |
| CN108220369B (zh) * | 2017-12-04 | 2021-03-12 | 广东双骏生物科技有限公司 | 一种生产重组水蛭素的方法 |
| US12084664B2 (en) | 2018-07-24 | 2024-09-10 | Wacker Chemie Ag | Bacterial lpp mutants and the use thereof for the secretory production of recombinant proteins |
| CN115572329B (zh) * | 2021-06-21 | 2024-02-06 | 王大勇 | 一组活性增强代谢较慢的菲牛蛭基因重组水蛭素及其制备方法 |
Family Cites Families (21)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE2936543A1 (de) * | 1979-09-10 | 1981-04-09 | Behringwerke Ag, 3550 Marburg | Chromogene verbindungen |
| AU561343B2 (en) * | 1981-10-19 | 1987-05-07 | Genentech Inc. | Human immune interferon by recombinant dna |
| CA1341414C (fr) * | 1984-03-27 | 2002-12-31 | Paul Tolstoshev | Vecteurs d'expression de l'hirudine, cellules transformees et procede de preparation de l'hirudine |
| DE3438296A1 (de) * | 1984-04-18 | 1985-11-07 | Hoechst Ag, 6230 Frankfurt | Neue polypeptide mit blutgerinnungshemmender wirkung, verfahren zu deren herstellung bzw. gewinnung, deren verwendung und diese enthaltende mittel |
| GB8412517D0 (en) * | 1984-05-16 | 1984-06-20 | Nagai K | Recombinant fusion proteins |
| EP0168342B1 (de) * | 1984-06-14 | 1991-07-03 | Ciba-Geigy Ag | Verfahren zur Herstellung von Thrombin-Inhibitoren |
| DE3429430A1 (de) * | 1984-08-10 | 1986-02-20 | Hoechst Ag, 6230 Frankfurt | Gentechnologisches verfahren zur herstellung von hirudin und mittel zur durchfuehrung dieses verfahrens |
| DE3445517C2 (de) * | 1984-12-13 | 1993-11-18 | Ciba Geigy | Für ein Hirudin-ähnliches Protein codierende DNA-Sequenz und Verfahren zur Herstellung eines Hirudin-ähnlichen Proteins |
| US4923808A (en) * | 1985-03-12 | 1990-05-08 | Genentech, Inc. | Method for identifying mutants secreting high levels of heterologous proteins |
| DE3900626A1 (de) * | 1985-04-11 | 1989-07-27 | Hoechst Ag | Hirudin-derivat |
| FR2593518B1 (fr) * | 1985-05-02 | 1989-09-08 | Transgene Sa | Vecteurs d'expression et de secretion de l'hirudine par les levures transformees |
| MY101203A (en) * | 1985-12-12 | 1991-08-17 | Ucp Gen Pharma Ag | Production of thrombin iinhibitors. |
| MC1834A1 (fr) * | 1986-07-10 | 1988-06-03 | Transgene Sa | Bloc fonctionnel d'adn et plasmide codant pour l'hirudine,levure transformee,procede de preparation de l'hirudine,hirudine obtenue et son utilisation |
| AU604925B2 (en) * | 1988-02-23 | 1991-01-03 | Schering Aktiengesellschaft | A hirudin derivative |
| DE3813107A1 (de) * | 1988-04-19 | 1989-11-02 | Consortium Elektrochem Ind | Sekretormutante von escherichia coli |
| GB8817160D0 (en) * | 1988-07-19 | 1988-08-24 | Ciba Geigy Ag | Novel proteins |
| JPH0648988B2 (ja) * | 1988-07-26 | 1994-06-29 | 工業技術院長 | トロンビン阻害物質の製造法 |
| FR2636643B1 (fr) * | 1988-08-24 | 1990-12-28 | Sanofi Sa | Peptide-signal, sequences d'adn codant pour celui-ci, vecteurs d'expression portant l'une de ces sequences et procede de production periplasmique d'un polypeptide |
| GB8826428D0 (en) * | 1988-11-11 | 1988-12-14 | Biopharm Ltd | Antithrombin |
| US5164304A (en) * | 1989-05-04 | 1992-11-17 | Sri International | Method and vectors for stabilizing hirudin and human laminin b1 expression |
| ES2129749T3 (es) * | 1990-11-08 | 1999-06-16 | Japan Energy Corp | Vector de secrecion, microorganismos transformados que contienen el indicado vector y produccion de un producto a partir de dicho microorganismo. |
-
1990
- 1990-03-22 DE DE4009268A patent/DE4009268A1/de not_active Withdrawn
-
1991
- 1991-02-21 IL IL9732391A patent/IL97323A/xx not_active IP Right Cessation
- 1991-02-22 PH PH42047A patent/PH30485A/en unknown
- 1991-02-28 FI FI911009A patent/FI100250B/fi not_active IP Right Cessation
- 1991-03-05 IE IE73891A patent/IE71174B1/en not_active IP Right Cessation
- 1991-03-14 NZ NZ237437A patent/NZ237437A/xx unknown
- 1991-03-14 NZ NZ245885A patent/NZ245885A/xx unknown
- 1991-03-18 KR KR1019910004246A patent/KR950007510B1/ko not_active IP Right Cessation
- 1991-03-19 ZA ZA912013A patent/ZA912013B/xx unknown
- 1991-03-20 JP JP3056714A patent/JPH07106157B2/ja not_active Expired - Fee Related
- 1991-03-21 AT AT91104422T patent/ATE135042T1/de not_active IP Right Cessation
- 1991-03-21 AU AU73680/91A patent/AU640212B2/en not_active Ceased
- 1991-03-21 CN CN91101687A patent/CN1067104C/zh not_active Expired - Fee Related
- 1991-03-21 HU HU91953A patent/HU214368B/hu not_active IP Right Cessation
- 1991-03-21 DE DE59107495T patent/DE59107495D1/de not_active Expired - Fee Related
- 1991-03-21 NO NO911137A patent/NO308543B1/no unknown
- 1991-03-21 ES ES91104422T patent/ES2084052T3/es not_active Expired - Lifetime
- 1991-03-21 DK DK91104422.0T patent/DK0448093T3/da active
- 1991-03-21 PT PT97093A patent/PT97093B/pt not_active IP Right Cessation
- 1991-03-21 BR BR919101104A patent/BR9101104A/pt not_active Application Discontinuation
- 1991-03-21 EP EP91104422A patent/EP0448093B1/de not_active Expired - Lifetime
- 1991-03-21 RU SU4894968A patent/RU2118365C1/ru not_active IP Right Cessation
- 1991-03-22 CA CA002038888A patent/CA2038888C/en not_active Expired - Fee Related
-
1992
- 1992-11-25 US US07/982,064 patent/US5919895A/en not_active Expired - Fee Related
-
1996
- 1996-05-17 GR GR960401313T patent/GR3019946T3/el unknown
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FG2A | Definitive protection |
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