IT1251680B - Clonaggio e sequenziamento dell'operone srfa di bacillus subtilis che codifica il complesso multienzimatico surfattina sintetasi - Google Patents
Clonaggio e sequenziamento dell'operone srfa di bacillus subtilis che codifica il complesso multienzimatico surfattina sintetasiInfo
- Publication number
- IT1251680B IT1251680B ITMI912683A ITMI912683A IT1251680B IT 1251680 B IT1251680 B IT 1251680B IT MI912683 A ITMI912683 A IT MI912683A IT MI912683 A ITMI912683 A IT MI912683A IT 1251680 B IT1251680 B IT 1251680B
- Authority
- IT
- Italy
- Prior art keywords
- bacillus subtilis
- surfactin
- operon
- multienzyme
- synthetase complex
- Prior art date
Links
- 101100257702 Bacillus subtilis (strain 168) srfAA gene Proteins 0.000 title abstract 4
- 101100257706 Dictyostelium discoideum srfA gene Proteins 0.000 title abstract 4
- 244000063299 Bacillus subtilis Species 0.000 title abstract 3
- 235000014469 Bacillus subtilis Nutrition 0.000 title abstract 3
- 108010034149 surfactin synthetase Proteins 0.000 title abstract 3
- 238000010367 cloning Methods 0.000 title 1
- 238000012163 sequencing technique Methods 0.000 title 1
- AFWTZXXDGQBIKW-UHFFFAOYSA-N C14 surfactin Natural products CCCCCCCCCCCC1CC(=O)NC(CCC(O)=O)C(=O)NC(CC(C)C)C(=O)NC(CC(C)C)C(=O)NC(C(C)C)C(=O)NC(CC(O)=O)C(=O)NC(CC(C)C)C(=O)NC(CC(C)C)C(=O)O1 AFWTZXXDGQBIKW-UHFFFAOYSA-N 0.000 abstract 2
- 108090000790 Enzymes Proteins 0.000 abstract 2
- 102000004190 Enzymes Human genes 0.000 abstract 2
- NJGWOFRZMQRKHT-UHFFFAOYSA-N surfactin Natural products CC(C)CCCCCCCCCC1CC(=O)NC(CCC(O)=O)C(=O)NC(CC(C)C)C(=O)NC(CC(C)C)C(=O)NC(C(C)C)C(=O)NC(CC(O)=O)C(=O)NC(CC(C)C)C(=O)NC(CC(C)C)C(=O)O1 NJGWOFRZMQRKHT-UHFFFAOYSA-N 0.000 abstract 2
- NJGWOFRZMQRKHT-WGVNQGGSSA-N surfactin C Chemical compound CC(C)CCCCCCCCC[C@@H]1CC(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@H](CC(C)C)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@H](CC(C)C)C(=O)N[C@@H](CC(C)C)C(=O)O1 NJGWOFRZMQRKHT-WGVNQGGSSA-N 0.000 abstract 2
- 108700039691 Genetic Promoter Regions Proteins 0.000 abstract 1
- 108700026244 Open Reading Frames Proteins 0.000 abstract 1
- 108020004511 Recombinant DNA Proteins 0.000 abstract 1
- 239000012634 fragment Substances 0.000 abstract 1
- 230000006698 induction Effects 0.000 abstract 1
Landscapes
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
L'operone srfA di Bacillus subtilis che codifica il complesso multienzimatico surfattina sintetasi responsabile della produzione della surfattina e dell'induzione dello stato di competenza di Bacillus subtilis è stato clonato e sequenziato.L'operone srfA è costituito da circa 27 kilobasi e comprende una regione promotore ed almeno quattro sequenze codificanti (Open Reading Frame-ORF) che sono tradotte separatamente e che codificano per quattro proteine enzimatiche differenti.L'operone srfA od almeno uno degli ORF di detto operone o regioni di questi sono utili per la produzione via DNA ricombinante di surfattina o di almeno una proteina enzimatica del complesso surfattina sintetasi o di frammenti di questa.
Priority Applications (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| ITMI912683A IT1251680B (it) | 1991-10-09 | 1991-10-09 | Clonaggio e sequenziamento dell'operone srfa di bacillus subtilis che codifica il complesso multienzimatico surfattina sintetasi |
| EP92203037A EP0540074A1 (en) | 1991-10-09 | 1992-10-03 | Cloning and sequencing of that chromosomal DNA region of bacillus subtilis comprising the SRFA operon which encodes the multienzymatic complex surfactin synthetase |
| JP4298004A JPH05292977A (ja) | 1991-10-09 | 1992-10-09 | バシラス・サチリス染色体ｄｎａ領域 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| ITMI912683A IT1251680B (it) | 1991-10-09 | 1991-10-09 | Clonaggio e sequenziamento dell'operone srfa di bacillus subtilis che codifica il complesso multienzimatico surfattina sintetasi |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| ITMI912683A0 ITMI912683A0 (it) | 1991-10-09 |
| ITMI912683A1 ITMI912683A1 (it) | 1993-04-09 |
| IT1251680B true IT1251680B (it) | 1995-05-19 |
Family
ID=11360840
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ITMI912683A IT1251680B (it) | 1991-10-09 | 1991-10-09 | Clonaggio e sequenziamento dell'operone srfa di bacillus subtilis che codifica il complesso multienzimatico surfattina sintetasi |
Country Status (1)
| Country | Link |
|---|---|
| IT (1) | IT1251680B (it) |
-
1991
- 1991-10-09 IT ITMI912683A patent/IT1251680B/it active IP Right Grant
Also Published As
| Publication number | Publication date |
|---|---|
| ITMI912683A1 (it) | 1993-04-09 |
| ITMI912683A0 (it) | 1991-10-09 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| 0001 | Granted | ||
| TA | Fee payment date (situation as of event date), data collected since 19931001 |
Effective date: 19971014 |