ES2070924T3 - Mutantes del factor beta-1 de crecimiento transformante de simio. - Google Patents

Mutantes del factor beta-1 de crecimiento transformante de simio.

Info

Publication number
ES2070924T3
ES2070924T3 ES89403294T ES89403294T ES2070924T3 ES 2070924 T3 ES2070924 T3 ES 2070924T3 ES 89403294 T ES89403294 T ES 89403294T ES 89403294 T ES89403294 T ES 89403294T ES 2070924 T3 ES2070924 T3 ES 2070924T3
Authority
ES
Spain
Prior art keywords
beta
tgf
cells
precursor
cdnas
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES89403294T
Other languages
English (en)
Inventor
Anthony F Purchio
Larry Gentry
Daniel Twardzik
Amy M Brunner
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Oncogen LP
Original Assignee
Oncogen LP
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Oncogen LP filed Critical Oncogen LP
Application granted granted Critical
Publication of ES2070924T3 publication Critical patent/ES2070924T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/475—Growth factors; Growth regulators
    • C07K14/495—Transforming growth factor [TGF]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Toxicology (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Microbiology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Control Of Eletrric Generators (AREA)
  • Transmission And Conversion Of Sensor Element Output (AREA)

Abstract

LA TRANSFORMACION RECOMBINANTE DEL FACTOR (BETA)-1 (TGF-(BETA)1) DE CRECIMIENTO, ES EXPRESADA A ALTOS NIVELES EN CELULAS (CHO) DE OVARIOS DE HAMSTERS CHINOS USANDO UNA AMPLIFICACION DEL GENE (DHFR) REDUCTOR DIHIDROFOLATO. LA EXPRESION LASMIDA ES DERIVADA DE LOS VECTORES PSV2 Y CONTENIDA EN TANDEM, AL DHFR CDNAS DE LOS RATONES. LA TRANSCRIPCION DE AMBOS CDNAS ES CONTROLADA POR UN PROMOTOR SV40. LOS PASOS DE SELECCION DE LA CELULA CHO TRANSFORMADAS EN CONCENTRACIONES AUMENTADAS DE LINEAS DE CELULAS RELACIONADAS EXPRESAN LAS SECUENCIAS DE ACIDO NUCLEICO TGF-(BETA)1 AMPLIFICADAS. LA EXPRESION PLASMIDA DEL ADN ES AMPLIADA A MAS DE 35 EN UNO DE LOS TRANSFECTANTES METROTEXATOS SELECCIONADOS. LA MAYORIA DE LAS PROTEINAS PROTEGIDAS EN ESTAS CELULAS DE POLIPEPTIDOS PRECURSORES TGF-(BETA)1. LOS NIVELES DE PROTEINA TGF-(BETA)1 RECOMBINANTE PROTEGIDA POR ESTAS CELULAS SON DE 30 MG/24 HORAS/10X7. LA ENCODIFICACION DE LA EXPRESION DE VECTOR DE PRECURSOR TGF-(BETA)1 DIRIGEN LA SINTESIS Y LA SECRECCIONDE TGF-(BETA)1 EN CELULAS MAMIFERAS TRANSFECTADAS.
ES89403294T 1988-12-16 1989-11-28 Mutantes del factor beta-1 de crecimiento transformante de simio. Expired - Lifetime ES2070924T3 (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US28591788A 1988-12-16 1988-12-16
US35017189A 1989-04-17 1989-04-17
US35372889A 1989-05-17 1989-05-17

Publications (1)

Publication Number Publication Date
ES2070924T3 true ES2070924T3 (es) 1995-06-16

Family

ID=27403570

Family Applications (1)

Application Number Title Priority Date Filing Date
ES89403294T Expired - Lifetime ES2070924T3 (es) 1988-12-16 1989-11-28 Mutantes del factor beta-1 de crecimiento transformante de simio.

Country Status (16)

Country Link
EP (1) EP0373994B1 (es)
JP (1) JPH03130089A (es)
KR (1) KR900009985A (es)
CN (1) CN1044125A (es)
AT (1) ATE118546T1 (es)
AU (1) AU638952B2 (es)
CA (1) CA2003886A1 (es)
DE (1) DE68921167T2 (es)
DK (1) DK638989A (es)
ES (1) ES2070924T3 (es)
FI (1) FI895730A0 (es)
IE (1) IE67811B1 (es)
IL (1) IL92489A0 (es)
NO (1) NO179676C (es)
NZ (1) NZ231581A (es)
PT (1) PT92454B (es)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0293785A3 (en) * 1987-05-29 1990-05-02 Oncogen Limited Partnership Cloning and expression of simian transforming growth factor-ss1
US5304541A (en) * 1988-12-15 1994-04-19 Bristol-Myers Squibb Company Methods using novel chimeric transforming growth factor-β1/β2
US7238711B1 (en) 1999-03-17 2007-07-03 Cambridge University Technical Services Ltd. Compounds and methods to inhibit or augment an inflammatory response
US6942853B2 (en) 2001-01-09 2005-09-13 Queen Mary And Westfield College Latent fusion protein
US7141392B2 (en) 2001-01-09 2006-11-28 Queen Mary And Westfield College Latent fusion protein
EP1279740A1 (en) * 2001-07-26 2003-01-29 Vrije Universiteit Brussel Recombinant vector derived from adeno-associated virus for gene therapy
WO2005068499A1 (en) * 2004-01-06 2005-07-28 The Government Of The United States As Representedby The Secretary, Department Of Health & Human Services Compositions and methods for the high efficiency expression of the transforming growth factor-beta supergene family
WO2012055541A1 (en) * 2010-10-25 2012-05-03 Bavarian Nordic A/S Tgf-beta in the development of conventional dendritic cells and uses thereof
CN104311631A (zh) * 2014-09-28 2015-01-28 苏州普罗达生物科技有限公司 转化生长因子β1激动剂多肽及其制备方法、应用
CN104211778A (zh) * 2014-09-28 2014-12-17 苏州普罗达生物科技有限公司 一种转化生长因子β1激动剂多肽及其制备方法、应用
RU2652312C2 (ru) * 2016-01-20 2018-04-25 Селл энд Джин Терапи Лтд Линейка биологически активных генно-терапевтических субстанций на основе гена tgfb1 для коррекции патологических состояний клеток органов и тканей и органов и тканей человека, способ получения и использования

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FI82266C (fi) * 1982-10-19 1991-02-11 Cetus Corp Foerfarande foer framstaellning av il-2 -mutein.
EP0293785A3 (en) * 1987-05-29 1990-05-02 Oncogen Limited Partnership Cloning and expression of simian transforming growth factor-ss1
CA2005120A1 (en) * 1988-12-15 1990-06-15 Anthony F. Purchio Tgf-beta 1/beta 2: a novel chimeric transforming growth factor-beta
CA2014699A1 (en) * 1989-04-20 1990-10-20 The Lubrizol Corporation Methods for reducing friction between relatively slideable components using metal overbased colloidal disperse systems

Also Published As

Publication number Publication date
IE893779L (en) 1990-06-16
CN1044125A (zh) 1990-07-25
ATE118546T1 (de) 1995-03-15
NO894759L (no) 1990-06-18
AU638952B2 (en) 1993-07-15
NO894759D0 (no) 1989-11-29
NO179676B (no) 1996-08-19
IL92489A0 (en) 1990-08-31
NZ231581A (en) 1993-01-27
CA2003886A1 (en) 1990-06-16
DE68921167T2 (de) 1995-06-01
DE68921167D1 (de) 1995-03-23
AU4588289A (en) 1990-06-21
IE67811B1 (en) 1996-05-01
FI895730A0 (fi) 1989-11-29
EP0373994A1 (en) 1990-06-20
EP0373994B1 (en) 1995-02-15
PT92454B (pt) 1995-07-18
DK638989D0 (da) 1989-12-15
KR900009985A (ko) 1990-07-06
DK638989A (da) 1990-06-17
PT92454A (pt) 1990-06-29
NO179676C (no) 1996-11-27
JPH03130089A (ja) 1991-06-03

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